Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,292,894 A→C L24R (CTG→CGG)  dsbE ← periplasmic thioredoxin of cytochrome c‑type biogenesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,292,8940AC100.0% 101.8 / NA 31L24R (CTG→CGG) dsbEperiplasmic thioredoxin of cytochrome c‑type biogenesis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/17);  total (14/17)

CGCAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGTTTGTAGTAAA  >  NC_000913/2292680‑2293094
                                                                                                                                                                                                                      |                                                                                                                                                                                                        
cgcAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTAAGCGCCCGCTGCCACAGAAccgcgtcgg                                                                                                                                                                                     >  2:5022/1‑231 (MQ=255)
 gcAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGc                                                                                                                                                                                    <  2:105476/236‑1 (MQ=255)
     aaaGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATc                                                                                                                                                                                >  1:710662/1‑236 (MQ=255)
         gttaatCAGATTGTGATGTTCCGCCAGGCAGTTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGc                                                                                                                                                                              <  2:610887/229‑1 (MQ=255)
             ttCAGATCTTGATGTTCCGCACGGCAGGTCGGACCCCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCa                                                                                                                                                                            <  2:740790/232‑1 (MQ=255)
             ttCAGATATGGATGGTCCGCAGGGCAGGTCGGACCCCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCa                                                                                                                                                                            <  1:268674/232‑1 (MQ=255)
                          gTTCCGGTCGGCAGGCCGGACCCCAGGTCGGCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATAACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGa                                                                                                                                                           <  2:710662/236‑1 (MQ=255)
                          gTTCCGCACGGAAGGTCGGACACCAGGTCGCCCGGACGTTAAGCAGTACTGGTTTGCCCTGAGTCATCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGa                                                                                                                                                           <  2:454763/236‑1 (MQ=255)
                            tCCGCACGGCAGGTCGGACCCCAGTCCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAAt                                                                                                                                                         <  2:352447/236‑1 (MQ=255)
                             ccGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGGGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAAtt                                                                                                                                                        >  1:225353/1‑236 (MQ=255)
                                 aCGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACa                                                                                                                                                    <  2:366427/236‑1 (MQ=255)
                                                           aGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGTGCTTCATACGGCCTcc                                                                                                                           >  2:67934/1‑235 (MQ=255)
                                                                            ggTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCg                                                                                                           <  1:5022/234‑1 (MQ=255)
                                                                                  cccTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGTCTCCGGCGCAGGTTTTTGTGGACTCa                                                                                                     >  2:691856/1‑234 (MQ=255)
                                                                                            gCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGgc                                                                                                                        >  2:148782/1‑205 (MQ=255)
                                                                                            gCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGgc                                                                                                                        <  1:148782/205‑1 (MQ=255)
                                                                                                 tCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGa                                                                                    <  1:67934/236‑1 (MQ=255)
                                                                                                                   cccGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGTTAGCGAGGGTCaaaacgac                                                                       >  2:747110/1‑226 (MQ=255)
                                                                                                                       gATTGTCCAGTTATTCGAGACCAAACTTCGGCACAGGCTTGCCAATGAGCGCCGAGTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTc                                                              <  1:747110/236‑1 (MQ=255)
                                                                                                                                 tGATTCGAAACCAAGCTGCGGCCCAGGCTTGCCAATGAGCCCCGATTCCAGATGGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGc                                                    <  2:663952/236‑1 (MQ=255)
                                                                                                                                      cGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGGGCTTCATACGGCCTCCGGCGAGGTTTTTTGCGGACTCACGCGCTTACGATAACGAGGATCAAACCGCCACAGCAGTCGGCCCAACGccgta                                                >  2:636839/1‑232 (MQ=255)
                                                                                                                                        agaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACAcag                                                                   <  2:548833/214‑1 (MQ=255)
                                                                                                                                        agaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACAcag                                                                   >  1:548833/1‑214 (MQ=255)
                                                                                                                                           cGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGGTTTTTGCGGACTCACTCGCTTACGATAGCGAGGCTCAAACAGACACAGCAGTTCGCCCAACGCCATCATCAg                                           >  2:150548/1‑235 (MQ=255)
                                                                                                                                                           gCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGa                                                                                    <  2:284119/178‑1 (MQ=255)
                                                                                                                                                           gCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGa                                                                                    >  1:284119/1‑178 (MQ=255)
                                                                                                                                                                ccAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGACCGCCCCAATCCAGCgc                     >  2:744169/1‑235 (MQ=255)
                                                                                                                                                                     gAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCAc                                                                                                    >  2:657847/1‑152 (MQ=255)
                                                                                                                                                                     gAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCAc                                                                                                    <  1:657847/152‑1 (MQ=255)
                                                                                                                                                                          ccGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGt            <  1:744169/235‑1 (MQ=255)
                                                                                                                                                                                    aTTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGTTTGTAGTaaa  >  2:375321/1‑235 (MQ=255)
                                                                                                                                                                                                                      |                                                                                                                                                                                                        
CGCAGAAAGCTGATTCAGATATTGATGTTCCGCACGGCAGGTCGGACACCAGGTCGCCCAGACGTTAAGCAGTACTGGTTTGCCCTGAGTCAGCACATCCGCCTGATAAAACTGCCCCGGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCGGACTCACGCGCTTACGATAGCGAGGATCAAACAGACACAGCAGTCCGCCCAACGCCATCATCAGCCCGCCCGCCCAAATCCAGCGAACAAATGGTTTGTAGTAAA  >  NC_000913/2292680‑2293094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A