Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,292,894	A→C	L24R (CTG→CGG)	dsbE ←	periplasmic thioredoxin of cytochrome c‑type biogenesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,292,894	0	A	C	96.4%	753.8 / 13.5	224	L24R (CTG→CGG)	dsbE	periplasmic thioredoxin of cytochrome c‑type biogenesis
Reads supporting (aligned to +/- strand): ref base A (2/6); new base C (108/108); total (110/114)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 224 total aligned reads displayed.

GGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCG  >  NC_000913/2292798‑2292989

ggATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCt                                                                                              >  1:4308587/1‑100 (MQ=255)

ggATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCt                                                                                              <  1:830482/100‑1 (MQ=255)

 gATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTTGTCGGATCATCCCCTTCGGCATTACGCGCCCGCtn                                                                                             <  1:1005717/100‑2 (MQ=255)

 gATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTg                                                                                             >  2:3734412/1‑100 (MQ=255)

   ttGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGcn                                                                                           <  1:4425527/100‑2 (MQ=255)

   ttGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGcc                                                                                           <  1:909662/100‑1 (MQ=255)

     gTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCcac                                                                                         <  2:198132/100‑1 (MQ=255)

     gTCCAGTGATTCGAGACGAAACTTCGGCACAGACTTGCGAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGGATTACGCGCCCGCTGCcac                                                                                         >  1:4956445/1‑100 (MQ=255)

      tCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCcaca                                                                                        >  1:1880474/1‑100 (MQ=255)

      tCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCcaca                                                                                        <  1:5112238/100‑1 (MQ=255)

       ccAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAcag                                                                                       <  2:120570/100‑1 (MQ=255)

       ccAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAcag                                                                                       >  2:2684286/1‑100 (MQ=255)

       ccAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAACGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAcag                                                                                       <  1:616148/100‑1 (MQ=255)

        cAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCcagatc                                                                                      >  2:2378280/1‑96 (MQ=255)

        cAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAcagc                                                                                      >  1:3544893/1‑100 (MQ=255)

        cAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAcagc                                                                                      >  1:3236682/1‑100 (MQ=255)

        cAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCAcagc                                                                                      >  2:1591444/1‑100 (MQ=255)

         aGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAcagca                                                                                     <  1:1772342/100‑1 (MQ=255)

           tGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACagcagc                                                                                   <  1:732455/100‑1 (MQ=255)

            gATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAgcg                                                                                  >  2:3636729/1‑100 (MQ=255)

                 gagaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCGCAGCAGCGCcgcg                                                                             >  1:5330184/1‑100 (MQ=255)

                  agaCGAAACTTCGGCACAGGCGTGCCAATGAGCGCCGATTCCAGATTGGGCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCgg                                                                            <  2:2130413/100‑1 (MQ=255)

                   gaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGc                                                                           >  1:1449593/1‑100 (MQ=255)

                   gaCGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGc                                                                           <  1:921880/100‑1 (MQ=255)

                     cGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCTCCCGCTGGCACAGCAGCGCCGCGGCaa                                                                         >  2:3571125/1‑100 (MQ=255)

                     cGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCaa                                                                         >  2:3045960/1‑100 (MQ=255)

                     cGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCaa                                                                         >  1:3829175/1‑100 (MQ=255)

                       aaaCTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATc                                                                       <  2:4902295/100‑1 (MQ=255)

                          cTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGcc                                                                    >  2:3211524/1‑100 (MQ=255)

                           ttCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCa                                                                   <  2:4406299/100‑1 (MQ=255)

                            tCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAg                                                                  <  1:4010031/100‑1 (MQ=255)

                            tCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAg                                                                  >  2:5300127/1‑100 (MQ=255)

                            tCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAg                                                                  <  2:3574690/100‑1 (MQ=255)

                            tCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAg                                                                  >  1:5028462/1‑100 (MQ=255)

                             cGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAgg                                                                 >  2:3270400/1‑100 (MQ=255)

                             cGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAgg                                                                 <  2:4269013/100‑1 (MQ=255)

                               gCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGaa                                                               >  2:2815611/1‑100 (MQ=255)

                                  caGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGAt                                                            <  1:1995387/100‑1 (MQ=255)

                                  caGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGAt                                                            >  1:4112722/1‑100 (MQ=255)

                                  caGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGAt                                                            >  1:3309199/1‑100 (MQ=255)

                                   aGGCTTGCCAATGAGCGCCGATTCCAGATTGTTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATa                                                           <  2:53444/100‑1 (MQ=255)

                                    ggCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCACCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATaa                                                          <  1:2653622/100‑1 (MQ=255)

                                     gCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAAt                                                         >  1:2497717/1‑100 (MQ=255)

                                          ccAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACg                                                    >  1:2707528/1‑100 (MQ=255)

                                          ccAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATCACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACg                                                    >  2:4176690/1‑100 (MQ=255)

                                           cAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACgg                                                   <  1:480607/100‑1 (MQ=255)

                                            aaTGGGCGCCGTTCCCAGATGGGCCGGATCATCCCCTTCGGCATTACGCGCCCGCGGCCACAGCGGCGCCGCGGCAATCGCCAGGAAGATAACCAACggn                                                  <  2:5420128/100‑2 (MQ=255)

                                              tGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCAGAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAAt                                                <  1:5286787/100‑1 (MQ=255)

                                              tGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAAt                                                >  1:162506/1‑100 (MQ=255)

                                                aGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCGCAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTa                                              <  1:4883367/100‑1 (MQ=255)

                                                aGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTa                                              >  2:647526/1‑100 (MQ=255)

                                                aGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTa                                              <  1:2070134/100‑1 (MQ=255)

                                                aGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAACAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTa                                              >  2:385704/1‑100 (MQ=255)

                                                 gcgcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTaa                                             <  1:2818354/100‑1 (MQ=255)

                                                 gcgcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTaa                                             >  2:2565207/1‑100 (MQ=255)

                                                  cgcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTaan                                            <  1:4410592/100‑2 (MQ=255)

                                                  cgcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAAc                                            >  2:3945350/1‑100 (MQ=255)

                                                  cgcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAAc                                            <  2:1051452/100‑1 (MQ=255)

                                                  cgcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAAc                                            <  1:3199585/100‑1 (MQ=255)

                                                   gcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACa                                           <  1:2254495/100‑1 (MQ=255)

                                                   gcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACa                                           >  2:3353866/1‑100 (MQ=255)

                                                   gcCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACa                                           >  1:2402957/1‑100 (MQ=255)

                                                    ccGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACaa                                          <  2:160935/100‑1 (MQ=255)

                                                       aTTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATAc                                       >  2:3250311/1‑100 (MQ=255)

                                                        ttCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACt                                      <  2:1335337/100‑1 (MQ=255)

                                                        ttCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACt                                      <  2:3461041/100‑1 (MQ=255)

                                                           cAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTg                                   >  1:1312534/1‑100 (MQ=255)

                                                            aGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTgc                                  <  2:4463850/100‑1 (MQ=255)

                                                            aGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTgc                                  >  2:2631622/1‑100 (MQ=255)

                                                             gATTGGTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTgcg                                 >  2:2527665/1‑100 (MQ=255)

                                                                 ggTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTc                             <  2:83739/100‑1 (MQ=255)

                                                                  gTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCa                            >  1:703450/1‑100 (MQ=255)

                                                                  gTCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCa                            >  1:3567867/1‑100 (MQ=255)

                                                                   tCGGATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCAt                           >  1:5175076/1‑100 (MQ=255)

                                                                    cGGATCAGCCCCTGCGGCATTACGCGCCCGCAGCCACCGCAGCGCCGCGGCAACCGCCAGGAAGATAATCAACGGAATTAACATTACTTTGCGCTTCATa                          <  2:3067813/100‑1 (MQ=255)

                                                                     ggATCATCCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATAc                         >  1:2275986/1‑100 (MQ=255)

                                                                      gATCATCCCCTTCGGCATTACGCGCCCGCTGCGACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACg                        >  2:4945767/1‑100 (MQ=255)

                                                                       atcatcCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACgg                       <  2:1749088/100‑1 (MQ=255)

                                                                        tcatcCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGc                      <  2:5242679/100‑1 (MQ=255)

                                                                         catcCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGcc                     <  1:3068880/100‑1 (MQ=255)

                                                                          atcCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCt                    >  2:4875863/1‑100 (MQ=255)

                                                                          atcCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCt                    >  1:465076/1‑100 (MQ=255)

                                                                           tcCCCTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTc                   >  2:5156455/1‑100 (MQ=255)

                                                                            ccccTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTcc                  <  2:2936818/100‑1 (MQ=255)

                                                                             cccTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCg                 <  1:3405366/100‑1 (MQ=255)

                                                                               cTTCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAAACGCCAGGAAGATAATCAACGGAATTAACAAGACTATGCGCTTCATACGGCCTCCGgc               <  2:3322775/100‑1 (MQ=255)

                                                                                ttCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGgcg              >  1:1841195/1‑100 (MQ=255)

                                                                                 tCGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGTCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGgcgc             <  2:1934321/100‑1 (MQ=255)

                                                                                  cGGCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCa            <  1:3117852/100‑1 (MQ=255)

                                                                                  cGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCa            <  1:1610968/100‑1 (MQ=255)

                                                                                   ggCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAg           >  1:3643103/1‑100 (MQ=255)

                                                                                   ggCATTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAg           >  2:1117066/1‑100 (MQ=255)

                                                                                      aTTACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGttt        >  1:2830122/1‑100 (MQ=255)

                                                                                        tACGCGCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGttttt      >  1:1190007/1‑100 (MQ=255)

                                                                                          cgcgCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTg    <  2:3403958/100‑1 (MQ=255)

                                                                                          cgcgCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTg    <  1:4567067/100‑1 (MQ=255)

                                                                                          cgcgCCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTg    >  2:891384/1‑100 (MQ=255)

                                                                                          cgcgCCCGCTGCCACAGCAGCGCCGCGGCAATAGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTg    >  2:4942364/1‑100 (MQ=255)

                                                                                           gcgcCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGc   >  1:2570048/1‑100 (MQ=255)

                                                                                           gcgcCCGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGc   <  1:2548190/100‑1 (MQ=255)

GGATTGTCCAGTGATTCGAGACGAAACTTCGGCACAGGCTTGCCAATGAGCGCCGATTCCAGATTGGTCGGATCATCCCCTTCGGCATTACGCGCCAGCTGCCACAGCAGCGCCGCGGCAATCGCCAGGAAGATAATCAACGGAATTAACAATACTTTGCGCTTCATACGGCCTCCGGCGCAGTTTTTTGCG  >  NC_000913/2292798‑2292989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A