Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F406 I3 R1 15 135.2 6912603 92.5% 6394157 99.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,089,222 G→A D212D (GAC→GAT)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,089,2220GA99.1% 332.3 / ‑4.7 106D212D (GAC→GAT) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (54/51);  total (54/52)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 106 total aligned reads displayed.

ATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGC  >  NC_000913/1089126‑1089314
                                                                                                |                                                                                            
aTTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATc                                                                                            <  1:1433201/99‑1 (MQ=255)
 tttACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCa                                                                                           <  1:66882/99‑1 (MQ=255)
 tttACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCa                                                                                           <  1:408417/99‑1 (MQ=255)
    aCCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCCTCCAGATGAATTCTTTCCCGCTACTCTGCTTCGCGTTCATACTGTTAGTGATCAGTa                                                                                        >  2:3140390/1‑99 (MQ=255)
    aCCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTa                                                                                        >  2:3076318/1‑99 (MQ=255)
     ccTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTaa                                                                                       >  2:2786029/1‑99 (MQ=255)
       tCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTaaaa                                                                                     >  1:3424350/1‑99 (MQ=255)
       tCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTaaaa                                                                                     <  2:305231/99‑1 (MQ=255)
       tCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTaaaa                                                                                     <  2:589002/99‑1 (MQ=255)
        cAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAat                                                                                    >  1:1160005/1‑99 (MQ=255)
        cAACCTTTGTACGCAGGTATTCCGTCATTTGTACAGCATCCAGACGAATCCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAat                                                                                    >  1:3348435/1‑99 (MQ=255)
            cTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGc                                                                                <  1:3103190/99‑1 (MQ=255)
             tttGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCa                                                                               >  1:2729060/1‑99 (MQ=255)
             tttGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCa                                                                               <  1:2688062/99‑1 (MQ=255)
              ttGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCAc                                                                              <  2:2772943/99‑1 (MQ=255)
               tGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCTGTTTCATACCGTGCGTGATCAGTAAAATATGcgcg                                                                             >  2:334224/1‑99 (MQ=255)
                 tACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAt                                                                           >  1:2555214/1‑99 (MQ=255)
                 tACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAt                                                                           >  2:482000/1‑99 (MQ=255)
                  aCGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAtt                                                                          >  2:1705245/1‑99 (MQ=255)
                  aCGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAtt                                                                          <  1:126958/99‑1 (MQ=255)
                   cGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGAttt                                                                         >  1:1563848/1‑99 (MQ=255)
                    gCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTa                                                                        >  1:694674/1‑99 (MQ=255)
                    gCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTa                                                                        >  2:2281209/1‑99 (MQ=255)
                    gCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTa                                                                        >  1:519422/1‑99 (MQ=255)
                     cAGGTATTCCGTCATTTTTACAGCATCAAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTAc                                                                       <  2:3335741/99‑1 (MQ=255)
                      aGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACa                                                                      <  1:2267416/99‑1 (MQ=255)
                       ggTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGGTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACat                                                                     >  1:566646/1‑99 (MQ=255)
                        gTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATGCCGTGCGTGATCAGTAAAATATGCACGATTTACata                                                                    >  1:3276555/1‑99 (MQ=255)
                        gTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACata                                                                    >  2:576241/1‑99 (MQ=255)
                          aTTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACAtata                                                                  <  1:1118025/99‑1 (MQ=255)
                           ttCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTATGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATAc                                                                 <  2:2831065/99‑1 (MQ=255)
                            tCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACa                                                                <  2:196694/99‑1 (MQ=255)
                             ccGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAg                                                               <  2:2870585/99‑1 (MQ=255)
                              cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAgg                                                              <  2:2151749/99‑1 (MQ=255)
                              cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAgg                                                              <  2:1312192/99‑1 (MQ=255)
                              cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAgg                                                              <  1:2005391/99‑1 (MQ=255)
                              cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAgg                                                              >  2:2568932/1‑99 (MQ=255)
                              cGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAgg                                                              >  2:1819940/1‑99 (MQ=255)
                               gTCATATTTACATCATCCAGACGAATTCTTTCCCGATATACTGCTGCGATTTCGTACCGTGCGTGATCAGTAAAATATGCACGATTTACATAGTCAGGc                                                             >  2:3218309/1‑99 (MQ=255)
                                 cATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCATTAAAATATGCACGATTTACATATACAGGCaa                                                           <  1:2681386/99‑1 (MQ=255)
                                 cATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCaa                                                           >  2:808569/1‑99 (MQ=255)
                                   tttttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATa                                                         >  1:1218952/1‑99 (MQ=255)
                                   tttttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATa                                                         >  1:2980125/1‑99 (MQ=255)
                                    ttttACAGCATCCAGGCGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATaa                                                        >  1:2198891/1‑99 (MQ=255)
                                     tttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAg                                                       >  2:3355212/1‑99 (MQ=255)
                                     tttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAg                                                       <  1:1330109/99‑1 (MQ=255)
                                     tttACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAg                                                       <  1:1725127/99‑1 (MQ=255)
                                      ttACAGCATCCAGACGAATTCTTTCCCGCTATTCTGCTGCGGTTTCGTACCGTGCGTGATCAGTAGAATATGCACGATTTACATATACAGGCAATAAGc                                                      <  2:3162910/99‑1 (MQ=255)
                                       tACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCt                                                     >  1:2743728/1‑99 (MQ=255)
                                        aCAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTg                                                    <  2:908325/99‑1 (MQ=255)
                                           gCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCg                                                 <  1:911271/99‑1 (MQ=255)
                                              tCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTg                                              <  2:2549273/99‑1 (MQ=255)
                                              tCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTg                                              <  2:362389/99‑1 (MQ=255)
                                              tCCAGAAGAATTCTTTCCCGGTCTTCTGCTGCGGTTTCATACAGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCAGGTg                                              <  2:1881131/99‑1 (MQ=255)
                                               ccAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTgg                                             <  1:816882/99‑1 (MQ=255)
                                               ccAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTgg                                             <  1:556825/99‑1 (MQ=255)
                                                 aGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTGAAATATGCACGATTTACAGATACAGGGAATAAGCTGCCGGTggag                                           >  1:3257483/1‑97 (MQ=255)
                                                 aGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCa                                           >  2:1540135/1‑99 (MQ=255)
                                                 aGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGACTTACATATACAGGCAATAAGCTGCCGGTGGCa                                           >  2:3424525/1‑99 (MQ=255)
                                                  gACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCAt                                          <  2:2985009/99‑1 (MQ=255)
                                                  gACAAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCAt                                          >  2:2490869/1‑99 (MQ=255)
                                                      aaTTCTTTCCCGTTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACTATTTACATATACAGGCAATAAGCTGCCTGTGGCATTAGc                                      <  2:1786487/99‑1 (MQ=255)
                                                      aaTTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGc                                      >  1:1219596/1‑99 (MQ=255)
                                                        ttCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCt                                    <  1:357541/99‑1 (MQ=255)
                                                         tCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTg                                   <  2:921472/99‑1 (MQ=255)
                                                          ctgtCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGa                                  <  2:2807493/96‑1 (MQ=255)
                                                                cGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGACTACCg                            >  2:2893519/1‑99 (MQ=255)
                                                                cGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCg                            >  2:2288119/1‑99 (MQ=255)
                                                                cGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCg                            <  2:2340905/99‑1 (MQ=255)
                                                                cGGTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCg                            >  2:2496622/1‑99 (MQ=255)
                                                                 ggTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGt                           >  2:1585181/1‑99 (MQ=255)
                                                                 ggTATTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGt                           <  2:946895/99‑1 (MQ=255)
                                                                    aTTCTGCTGCGGTTTCATACGGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGt                        <  2:1349930/99‑1 (MQ=255)
                                                                    aTTCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGt                        <  1:2333770/99‑1 (MQ=255)
                                                                      tCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTga                      >  1:3345487/1‑99 (MQ=255)
                                                                      tCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTga                      >  1:3345323/1‑99 (MQ=255)
                                                                      tCTGCTGCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTga                      <  2:545605/99‑1 (MQ=255)
                                                                       ctgctgCGGTTTCATACCGTGCGTGATCATTAAAATATGCACGAGTTACATATACATGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTgag                     >  1:3024253/1‑99 (MQ=255)
                                                                       ctgctgCGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTgag                     <  1:2920051/99‑1 (MQ=255)
                                                                         gctgcGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGaa                   >  1:1060763/1‑99 (MQ=255)
                                                                         gctgcGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGaa                   <  1:1801925/99‑1 (MQ=255)
                                                                         cctgcGGTTTCGTACCGAGCGTGATCACTAAAATATGCACGATTAACAAATACAGGCAATAAGATGACGGTGGCATTAGCCTCAATACCGTAGTGAGaa                   <  2:2337140/98‑1 (MQ=255)
                                                                          ctgcGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAAt                  >  1:1446445/1‑99 (MQ=255)
                                                                             cGGTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTcc               >  2:276700/1‑99 (MQ=255)
                                                                              ggTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCa              >  1:1571931/1‑99 (MQ=255)
                                                                               gTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTTGCATTAGCCTGAATACCGTAGTGAGAATTCCAt             <  2:1497221/99‑1 (MQ=255)
                                                                               gTTTCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCAt             >  2:790277/1‑99 (MQ=255)
                                                                                tttCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATg            >  1:188907/1‑99 (MQ=255)
                                                                                 ttCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGt           <  2:1203404/99‑1 (MQ=255)
                                                                                  tCATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTa          >  1:1082369/1‑99 (MQ=255)
                                                                                   cATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTAt         <  2:2764047/99‑1 (MQ=255)
                                                                                   cATACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTAt         <  1:2976872/99‑1 (MQ=255)
                                                                                    aTACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATg        <  1:2903710/99‑1 (MQ=255)
                                                                                    aTACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATg        <  1:768743/99‑1 (MQ=255)
                                                                                    aTACCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATg        >  2:1604869/1‑99 (MQ=255)
                                                                                      aCCGTGCGTGATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATgag      >  1:1462992/1‑99 (MQ=255)
                                                                                        cgtgcgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGaa    >  2:350741/1‑99 (MQ=255)
                                                                                         gtgcgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAg   >  1:3336804/1‑99 (MQ=255)
                                                                                         gtgcgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAg   >  1:1004271/1‑99 (MQ=255)
                                                                                          tgcgtgATCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGc  <  1:355802/99‑1 (MQ=255)
                                                                                                |                                                                                            
ATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGC  >  NC_000913/1089126‑1089314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A