breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 354,812 C→T intergenic (+220/‑110) prpE → / → codB propionate‑‑CoA ligase/cytosine transporter
RA 644,734 G→T A90A (GCC→GCA rna ← ribonuclease I
RA 1,089,222 G→A D212D (GAC→GAT pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
JC JC 1,292,991 +T :: IS1 (–) +7 bp intergenic (‑69/‑530) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 family protein, nitrate‑inducible
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,774,470 A→G M385V (ATG→GTG)  yfjW → CP4‑57 prophage; putative inner membrane protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,798,659 (T)8→7 coding (654/1074 nt) waaU ← lipopolysaccharide core biosynthesis
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 4,183,597 G→T D785Y (GAC→TAC)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1995402 1998493 3092 119 [2] [2] 171 yecF–yecS yecF,sdiA,yecC,yecS
* * ÷ NC_000913 3423700–3424234 3424606–3424238 5–907 64 [61] [62] 64 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =20 (0.150)88 (0.780) 61/164 0.7 84.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 16 (0.140)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780521 (0.160)91 (0.810) 61/164 0.7 84.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 16 (0.140)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)124 (0.960) 74/188 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19954012 (0.010)117 (0.870) 81/196 0.4 98.3% intergenic (‑42/‑417) uvrY/yecF response regulator in two‑component regulatory system with BarA/DUF2594 family protein
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1998494 =2 (0.010)167 (1.240) 77/196 0.5 98.8% coding (987/987 nt) dcyD D‑cysteine desulfhydrase, PLP‑dependent
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region