Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I1 R1 13 65.4 2035110 96.5% 1963881 151.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 747,460 G→A R104C (CGC→TGC)  abrB ← regulator of aidB expression; inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913747,4600GA100.0% 141.4 / NA 37R104C (CGC→TGC) abrBregulator of aidB expression; inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/16);  total (21/16)

GTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCGCGCATACTAAAGATGATCCCGGCGATCATCGGCCCAAGTAATA  >  NC_000913/747297‑747684
                                                                                                                                                                   |                                                                                                                                                                                                                                
gTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGAc                                                                                                                                                       >  1:137662/1‑239 (MQ=255)
gTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGAc                                                                                                                                                       >  2:110040/1‑239 (MQ=255)
gTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGAACGGCCAGTTGAc                                                                                                                                                       >  2:1425/1‑239 (MQ=255)
       gAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGaaa                                                                                                                                                                                                  >  1:471476/1‑189 (MQ=255)
       gAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGaaa                                                                                                                                                                                                  <  2:471476/189‑1 (MQ=255)
         aCAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCa                                                                                                                                                  <  1:110040/235‑1 (MQ=255)
                      cgacgaACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAGGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGaa                                                                                                                                 <  1:877968/239‑1 (MQ=255)
                                                 tAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTAAGGTTTTCGCCAATGATGCa                                                                                                       >  2:806814/1‑238 (MQ=255)
                                                    aCGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAAt                                                                                                                                                                                                                           >  2:465705/1‑119 (MQ=255)
                                                    aCGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAAt                                                                                                                                                                                                                           <  1:465705/119‑1 (MQ=255)
                                                         accaGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCa                                                                                                                                                                                                                             <  2:861746/112‑1 (MQ=255)
                                                         accaGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCa                                                                                                                                                                                                                             >  1:861746/1‑112 (MQ=255)
                                                         accaGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAAt                                                                                              >  1:360795/1‑239 (MQ=255)
                                                                         cgcCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAgg                                                                                                                                                >  1:554303/1‑173 (MQ=255)
                                                                              tAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCacac                                                                                                                                                                                                                               >  1:280677/1‑89 (MQ=255)
                                                                              tAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCacac                                                                                                                                                                                                                               <  2:280677/89‑1 (MQ=255)
                                                                              tAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGgcg                                                                                                                                                                                                         >  2:891391/1‑111 (MQ=255)
                                                                              tAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGgcg                                                                                                                                                                                                         <  1:891391/111‑1 (MQ=255)
                                                                               aaTCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCa                                                                        <  2:360795/239‑1 (MQ=255)
                                                                                   ttGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCgag                                                                               >  2:151896/1‑228 (MQ=255)
                                                                                   ttGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCgag                                                                               <  1:151896/228‑1 (MQ=255)
                                                                                                aCCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCag                                                       >  2:582568/1‑239 (MQ=255)
                                                                                                aCCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCag                                                       >  2:592953/1‑239 (MQ=255)
                                                                                                            gcgccgcCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCcgcg                                           <  1:582568/239‑1 (MQ=255)
                                                                                                             cgccgcCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCgcgc                                          >  2:652734/1‑239 (MQ=255)
                                                                                                              gccgccAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCGCGCa                                         <  1:652734/239‑1 (MQ=255)
                                                                                                              gccgccAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCGCGCa                                         <  1:806814/239‑1 (MQ=255)
                                                                                                                                ccAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTg                                                                                                                           >  1:517006/1‑139 (MQ=255)
                                                                                                                                ccAGGCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTg                                                                                                                           <  2:517006/139‑1 (MQ=255)
                                                                                                                                   ggCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTgg                                                                                       >  1:623066/1‑172 (MQ=255)
                                                                                                                                   ggCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTgg                                                                                       <  2:623066/172‑1 (MQ=255)
                                                                                                                                    gCACCGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCGCGCATACTAAAGATGATCCCGGCGat                   >  1:165408/1‑239 (MQ=255)
                                                                                                                                        cGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCgggg                                                                <  1:232678/190‑1 (MQ=255)
                                                                                                                                        cGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCgggg                                                                >  2:232678/1‑190 (MQ=255)
                                                                                                                                        cGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTg                                                          >  1:169117/1‑196 (MQ=255)
                                                                                                                                        cGGTATTTCCAGGCAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTg                                                          <  2:169117/196‑1 (MQ=255)
                                                                                                                                                     cAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGggcg                                                                                    <  2:943242/157‑1 (MQ=255)
                                                                                                                                                     cAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGggcg                                                                                    >  1:943242/1‑157 (MQ=255)
                                                                                                                                                     cAATGAGCTATAGCACACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCGCGCATACTAAAGATGATCCCGGCGATCATCGGCCCACGTAATa  >  2:87453/1‑239 (MQ=255)
                                                                                                                                                                   |                                                                                                                                                                                                                                
GTGACCAGAACAGCGGCCCCCGCGACGAACAGCACTCGCAGATATTGCATAAACGCCACCAGGCGAATATCTGCGCCGTAATCTTGCGCCATAGCGACCATTGCCGCCGCGCCGCCAGGAGAGGAGCCCCAGGCACCGGTATTTCCAGGCAATGAGCTATAGCGCACCAATAACCAACCCACAATGGCGCTGGAAAGCAGCGTTACCAGTAAAATTGCAAGCACGATCGGCCAGTTGACGGCCAGGGTGGTGAGAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGGGCGGCGAGAAAAGCAGAGCGGGGAAGTTGCAGAGTAATTCCGCGCATACTAAAGATGATCCCGGCGATCATCGGCCCAAGTAATA  >  NC_000913/747297‑747684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A