Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F418 I3 R1	16	172.0	8631016	94.8%	8182203	99.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,628,644	A→T	C99S (TGT→AGT)	yfgF ←	cyclic‑di‑GMP phosphodiesterase, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,628,644	0	A	T	96.5%	456.4 / ‑0.0	141	C99S (TGT→AGT)	yfgF	cyclic‑di‑GMP phosphodiesterase, anaerobic
Reads supporting (aligned to +/- strand): ref base A (2/3); new base T (60/76); total (62/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCC  >  NC_000913/2628548‑2628740

acacAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACtga                                                                                                <  1:3591216/99‑1 (MQ=255)

 cacaATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACtgag                                                                                               >  1:842100/1‑99 (MQ=255)

  acaATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTgaga                                                                                              >  2:759463/1‑99 (MQ=255)

  acaATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTgaga                                                                                              >  2:2362618/1‑99 (MQ=255)

  acaATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTgaga                                                                                              <  1:365413/99‑1 (MQ=255)

   caATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaa                                                                                             <  1:4310173/99‑1 (MQ=255)

    aaTCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaat                                                                                            <  1:982647/99‑1 (MQ=255)

    aaTCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaat                                                                                            >  1:1705759/1‑99 (MQ=255)

      tCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatga                                                                                          >  2:1450032/1‑99 (MQ=255)

       cTGGCAGAATATAGGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgtt                                                                                         <  2:3352553/99‑3 (MQ=255)

        tGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCTCCAACTGAGaatgata                                                                                        >  1:3051543/1‑99 (MQ=255)

          ccAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgataat                                                                                      <  2:1567967/99‑1 (MQ=255)

          ccAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgataat                                                                                      <  1:2054345/99‑1 (MQ=255)

             gAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgataatgat                                                                                   <  2:2076469/99‑1 (MQ=255)

             gAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgataatgat                                                                                   <  2:2901638/99‑1 (MQ=255)

             gAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgataatgat                                                                                   >  1:2681177/1‑99 (MQ=255)

             gAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGaatgataatgat                                                                                   <  2:378737/99‑1 (MQ=255)

              aaTATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAatgataatgata                                                                                  >  2:895346/1‑99 (MQ=255)

              aaTATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAatgataatgata                                                                                  >  2:1092622/1‑99 (MQ=255)

              aaTATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAatgataatgata                                                                                  <  2:1209403/99‑1 (MQ=255)

               atatACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAAtgataatgataa                                                                                 <  1:3498160/99‑1 (MQ=255)

                tataCGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATgataatgataat                                                                                >  1:43295/1‑99 (MQ=255)

                 ataCGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGataata                                                                               >  2:3195127/1‑99 (MQ=255)

                   aCGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCCACAGAGAATGATAATGATAATaaa                                                                             >  1:2877696/1‑99 (MQ=255)

                   aCGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATaaa                                                                             <  2:1829292/99‑1 (MQ=255)

                    cGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATaaaa                                                                            <  2:1614954/99‑1 (MQ=255)

                     gCTGGGAAATTAAACGGGTATCACCATGTGAAACATGGTTACGCCGGGGCGGAAAGACCCTGTAACAGCCCCAACTGAGAATGATAATGATAATAAAAt                                                                           >  2:4173855/1‑99 (MQ=255)

                      cTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAAtt                                                                          <  2:901719/99‑1 (MQ=255)

                       tGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTa                                                                         <  2:3273785/99‑1 (MQ=255)

                        gggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAg                                                                        <  1:157158/99‑1 (MQ=255)

                         ggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGc                                                                       <  2:2947004/99‑1 (MQ=255)

                         ggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGc                                                                       <  1:2654916/99‑1 (MQ=255)

                          gAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGcc                                                                      >  1:3597821/1‑99 (MQ=255)

                          gAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGcc                                                                      >  1:119071/1‑99 (MQ=255)

                           aaaTTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCg                                                                     <  1:2393748/99‑1 (MQ=255)

                            aaTTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGt                                                                    <  1:3533172/99‑1 (MQ=255)

                              ttAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGCTAATGATAATAAAATTAGCCGTTa                                                                  >  1:539516/1‑99 (MQ=255)

                              ttAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAACTTCGCCGTTa                                                                  >  1:539592/1‑99 (MQ=255)

                               tAAACGGGTATCGCCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTaa                                                                 <  2:368326/99‑1 (MQ=255)

                                aaaCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAAt                                                                <  1:1017138/99‑1 (MQ=255)

                                aaaCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAAt                                                                >  1:2371093/1‑99 (MQ=255)

                                 aaCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATg                                                               >  2:1324969/1‑99 (MQ=255)

                                 aaCGGGCATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATg                                                               >  2:3522318/1‑99 (MQ=255)

                                  aCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGa                                                              >  1:716506/1‑99 (MQ=255)

                                  aCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGa                                                              <  1:2513724/99‑1 (MQ=255)

                                         tCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGtt                                                       <  1:311025/99‑1 (MQ=255)

                                           aCCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTc                                                     >  1:255163/1‑99 (MQ=255)

                                           aCCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTc                                                     >  1:1511617/1‑99 (MQ=255)

                                            ccATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCg                                                    <  2:4098830/99‑1 (MQ=255)

                                            ccATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCg                                                    <  2:2945500/99‑1 (MQ=255)

                                             cATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGt                                                   <  1:3891005/99‑1 (MQ=255)

                                              aTGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTa                                                  <  2:3796842/99‑1 (MQ=255)

                                               tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaa                                                 <  1:2879022/99‑1 (MQ=255)

                                                 tgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaaa                                               <  1:738390/99‑1 (MQ=255)

                                                  gAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTGATGATAGCGTTTCGTaaaaa                                              >  2:1271780/1‑99 (MQ=255)

                                                  gAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaaaa                                              <  1:4140525/99‑1 (MQ=255)

                                                  gAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaaaa                                              <  1:1150827/99‑1 (MQ=255)

                                                  gAAACGTTGTTACGACGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATACTGATAACAAAATTAGCAGTTAATGATAGCGTTTAGTaaaaa                                              >  2:1461406/1‑99 (MQ=255)

                                                    aaCGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAcc                                            <  1:2166788/99‑1 (MQ=255)

                                                      cGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAcccn                                          <  2:2861267/99‑2 (MQ=255)

                                                      cGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCa                                          <  1:1219407/99‑1 (MQ=255)

                                                            tACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATCAGCCGTTAATGATAGCGCTTCGTAAAAACCCAGCTCTg                                    >  2:3889939/1‑99 (MQ=255)

                                                             aCGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGc                                   >  1:1964751/1‑99 (MQ=255)

                                                             aCGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGc                                   >  1:1108393/1‑99 (MQ=255)

                                                              cGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCa                                  <  2:2751276/99‑1 (MQ=255)

                                                                 cGGGGAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                               >  1:1579281/1‑99 (MQ=255)

                                                                  ggggAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                              >  2:1209274/1‑99 (MQ=255)

                                                                   gggAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAttt                             <  2:1876857/99‑1 (MQ=255)

                                                                   gggAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAttt                             <  2:639230/99‑1 (MQ=255)

                                                                    ggAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTg                            >  1:873043/1‑99 (MQ=255)

                                                                     gAGTAAAGACCCTGTAACTGCCGCAACTGAGTGTGATAATGATTATAAAATTACCCGTTAATAATAAAGATTAGTAAAAACCCACCTCTGCATATTTGc                           >  2:1538581/1‑99 (MQ=255)

                                                                     gAGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc                           <  1:3514828/99‑1 (MQ=255)

                                                                     gAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc                           <  2:3408062/99‑1 (MQ=255)

                                                                      aGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCg                          <  1:2242592/99‑1 (MQ=255)

                                                                      aGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCg                          <  2:1287431/99‑1 (MQ=255)

                                                                      aGTAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCg                          <  1:1530731/99‑1 (MQ=255)

                                                                        tAAAGGCCCTGTAACCGCCCCAACTGAGAATGATAATGATAATTAAATTTAGCGTTAATGATAGCGTTTCGTAAAGCCCCAGCTCTGCATATTTGCGGa                        <  2:3090954/99‑1 (MQ=255)

                                                                        tAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGa                        <  2:2640914/99‑1 (MQ=255)

                                                                        tAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGa                        >  1:3245282/1‑99 (MQ=255)

                                                                        tAAAGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGa                        <  1:337740/99‑1 (MQ=255)

                                                                         aacgACTCTGTAACCGCCCCAACTTAGAATCGTAATGATAATAAAAGGAGGCGTTAATGATAGCGTTTCGTGAAAACCCAGCTCTGCATATTTTCCGAc                       <  2:4023941/96‑1 (MQ=255)

                                                                         aaaGACCCTGTAACCGCCCCAACTGAGAATGCTAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGAc                       >  2:3844429/1‑99 (MQ=255)

                                                                          aaGACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACa                      >  2:299052/1‑99 (MQ=255)

                                                                            gACCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                    >  2:2123867/1‑99 (MQ=255)

                                                                             aCCCTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaaa                   <  1:2600196/99‑1 (MQ=255)

                                                                               ccTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAAt                 >  2:3131406/1‑99 (MQ=255)

                                                                               ccTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAAt                 <  2:2379632/99‑1 (MQ=255)

                                                                               ccTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAAt                 <  2:70127/99‑1 (MQ=255)

                                                                               ccTGTAACCGCCCCAACAGAGAATGATAATTATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATGTCTTCGGACAAAAAt                 >  1:3239471/1‑99 (MQ=255)

                                                                                cTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATc                <  1:2026633/99‑1 (MQ=255)

                                                                                cTGTAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATc                >  2:670887/1‑99 (MQ=255)

                                                                                   tAACCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt             <  1:1785458/99‑1 (MQ=255)

                                                                                     aCCGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTaa           <  1:1635036/99‑1 (MQ=255)

                                                                                      ccGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAg          >  2:1130773/1‑99 (MQ=255)

                                                                                      ccGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAg          <  2:2840366/99‑1 (MQ=255)

                                                                                       cGCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg         >  2:1359457/1‑99 (MQ=255)

                                                                                        gCCCCAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAACCCCAGCTCTGCACATTTGCGGACAAAAATCCCTAAGGc        >  2:1890195/1‑99 (MQ=255)

                                                                                         ccccAACTGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg       >  1:590248/1‑99 (MQ=255)

                                                                                           ccAACAGAGAAAGATAATGATAATAAACTTAGCCGTTAATGAAAAAAAAAAAAAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGAt     <  2:3489820/99‑1 (MQ=255)

                                                                                              aCAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATccc  <  1:3408503/99‑1 (MQ=255)

ACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCC  >  NC_000913/2628548‑2628740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A