Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F418 I3 R1 16 172.0 8631016 94.8% 8182203 99.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,132,227 C→A A798E (GCG→GAG)  metL → Bifunctional aspartokinase/homoserine dehydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,132,2270CA97.2% 588.6 / ‑2.2 182A798E (GCG→GAG) metLBifunctional aspartokinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (92/85);  total (93/89)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

CGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAAT  >  NC_000913/4132131‑4132322
                                                                                                |                                                                                               
 gTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGAt                                                                                              >  1:3927180/1‑99 (MQ=255)
 gTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGAt                                                                                              >  2:2593582/1‑99 (MQ=255)
 gTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGAt                                                                                              >  1:4296262/1‑99 (MQ=255)
  tGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGAtt                                                                                             >  1:3733245/1‑99 (MQ=255)
  tGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGAtt                                                                                             <  1:902370/99‑1 (MQ=255)
    cGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATGACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCa                                                                                           <  2:4187999/99‑1 (MQ=255)
     gATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAg                                                                                          >  2:790060/1‑99 (MQ=255)
      aTAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGt                                                                                         <  2:734736/99‑1 (MQ=255)
      aTAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGt                                                                                         >  2:624895/1‑99 (MQ=255)
      aTAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGt                                                                                         >  2:3885875/1‑99 (MQ=255)
      aTAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGt                                                                                         >  1:3519606/1‑99 (MQ=255)
      aTAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCCCTGGGCGCGACGTCACCGCCGGGGAGGTTCAGt                                                                                         >  2:4034502/1‑99 (MQ=255)
       tAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTc                                                                                        >  1:1310381/1‑99 (MQ=255)
       tAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTc                                                                                        <  1:1412895/99‑1 (MQ=255)
        aaCGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCg                                                                                       <  1:984746/99‑1 (MQ=255)
        aaCGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCg                                                                                       <  1:3747648/99‑1 (MQ=255)
         aCGTCTTTGCCATCGAGAGCCGCTGGTATCGCGGTAGCCCTCTGGTGATCCGCGGACTTGGCGCTGGGCGCGACGTCCCCGCCGGGGCGATTCAGTCgg                                                                                      <  2:1944037/99‑1 (MQ=255)
         aCGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCgg                                                                                      >  2:2172125/1‑99 (MQ=255)
           gTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGat                                                                                    <  1:1169213/99‑1 (MQ=255)
            tCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGata                                                                                   >  1:3010179/1‑99 (MQ=255)
             cTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGatat                                                                                  <  2:3082675/99‑1 (MQ=255)
             cTTTGCCATCAAAAGCCGCTGGGATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGatat                                                                                  <  2:597284/99‑1 (MQ=255)
              tttGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATc                                                                                 <  2:539580/99‑1 (MQ=255)
              tttGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATc                                                                                 <  2:1752105/99‑1 (MQ=255)
               ttGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCa                                                                                >  2:1147339/1‑99 (MQ=255)
               ttGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCa                                                                                >  2:2988068/1‑99 (MQ=255)
                  ccATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAAcc                                                                             >  1:3020729/1‑99 (MQ=255)
                   cATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCg                                                                            >  1:2829624/1‑99 (MQ=255)
                   cATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCg                                                                            >  2:1095285/1‑99 (MQ=255)
                   cATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCg                                                                            >  1:4162732/1‑99 (MQ=255)
                    aTCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCgg                                                                           >  1:477038/1‑99 (MQ=255)
                     tCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGc                                                                          <  2:2043733/99‑1 (MQ=255)
                       gAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTTGCGCTGTGCGCGACGTCACCGCCGGGGCGATTCAGTCGGTTATCAACCGGCTg                                                                        <  2:3986389/99‑1 (MQ=255)
                        aaaGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCTCTGCCGGGGAGATTCAGTCGGATATCAACCGGCTgg                                                                       <  2:4164376/99‑1 (MQ=255)
                        aaaGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTgg                                                                       >  1:4231018/1‑99 (MQ=255)
                        aaaGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTgg                                                                       >  1:4230917/1‑99 (MQ=255)
                        aaaGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTgg                                                                       >  2:1652894/1‑99 (MQ=255)
                        aaaGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTgg                                                                       >  1:2638645/1‑99 (MQ=255)
                        aaaGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTgg                                                                       >  1:3756401/1‑99 (MQ=255)
                           gCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGcac                                                                    >  1:3098241/1‑99 (MQ=255)
                            ccGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGcaca                                                                   >  1:1258871/1‑99 (MQ=255)
                             cGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAg                                                                  <  1:4285296/99‑1 (MQ=255)
                              gCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgt                                                                 >  1:3878071/1‑99 (MQ=255)
                              gCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgt                                                                 >  2:1341266/1‑99 (MQ=255)
                                tGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgttg                                                               <  1:3331500/99‑1 (MQ=255)
                                tGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgttg                                                               <  2:2143292/99‑1 (MQ=255)
                                 ggTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgttgt                                                              <  2:955864/99‑1 (MQ=255)
                                  gTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgttgtt                                                             >  1:3731547/1‑99 (MQ=255)
                                  gTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAgttgtt                                                             <  2:1289308/99‑1 (MQ=255)
                                  gTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCGGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGTTGGCACAgttgtt                                                             <  1:3090772/99‑1 (MQ=255)
                                   tATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGttgttg                                                            <  1:1091003/99‑1 (MQ=255)
                                   tATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGttgttg                                                            >  1:1790299/1‑99 (MQ=255)
                                    aTCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTtgttgt                                                           >  1:1373481/1‑99 (MQ=255)
                                    aTCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTtgttgt                                                           <  2:3107907/99‑1 (MQ=255)
                                     tCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTa                                                          >  1:150699/1‑99 (MQ=255)
                                     tCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTa                                                          >  1:4099805/1‑99 (MQ=255)
                                     tCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTa                                                          >  2:3474524/1‑99 (MQ=255)
                                        cgATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAAtt                                                       <  1:1046383/99‑1 (MQ=255)
                                          aTAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTc                                                     <  2:408953/99‑1 (MQ=255)
                                               ccTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCGGGGGAGAGTCAGTCGGATATCGACCTGCTGGCACAGTTGTTGTAGTTTCAGaaa                                                >  2:3181577/1‑99 (MQ=255)
                                               ccTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGaaa                                                >  1:530821/1‑99 (MQ=255)
                                                ctctGGTGATCCGCGGACCTGGCGCTGGGGGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAAt                                               <  2:343895/99‑1 (MQ=255)
                                                ctctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTACTTTCAGAAAt                                               >  2:925472/1‑99 (MQ=255)
                                                 tctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAAtt                                              >  1:752590/1‑99 (MQ=255)
                                                 tctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAAtt                                              >  1:2021835/1‑99 (MQ=255)
                                                 tctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAAtt                                              <  1:772917/99‑1 (MQ=255)
                                                    ggTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTtaa                                           >  1:319008/1‑99 (MQ=255)
                                                    ggTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTtaa                                           >  1:3137183/1‑99 (MQ=255)
                                                    ggTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTtaa                                           <  1:209390/99‑1 (MQ=255)
                                                     gTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTtaat                                          <  1:570161/99‑1 (MQ=255)
                                                     gTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTtaat                                          >  1:559764/1‑99 (MQ=255)
                                                      tGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTtaata                                         >  1:1295045/1‑99 (MQ=255)
                                                         tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATCCAGTCGGATATCAACCGGCTGGCACATTTGTTGTAATTTCAGCAATCTCATAATg                                      <  2:3522270/99‑1 (MQ=255)
                                                            gcgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGccc                                   >  1:3595805/1‑99 (MQ=255)
                                                              ggACCTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCgg                                 <  1:2199648/99‑1 (MQ=255)
                                                                  cTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACt                             >  2:2447255/1‑99 (MQ=255)
                                                                  cTGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACt                             >  1:1655261/1‑99 (MQ=255)
                                                                   tGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTc                            <  1:362975/99‑1 (MQ=255)
                                                                   tGGCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTc                            >  1:2677795/1‑99 (MQ=255)
                                                                    ggCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCa                           <  2:3292748/99‑1 (MQ=255)
                                                                    ggCGCTGGGCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCa                           <  2:1115838/99‑1 (MQ=255)
                                                                           ggCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTc                    <  2:3809645/99‑1 (MQ=255)
                                                                           ggCGCGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTc                    >  1:2769429/1‑99 (MQ=255)
                                                                            gcgcGACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCg                   <  2:3571889/99‑1 (MQ=255)
                                                                             ggcgACGTCGCCGCCGGGGCGATTAAGTAGGATATCAACCGGCTGGTAGAGTTGTTGTATTTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCgg                  <  2:3926804/98‑1 (MQ=255)
                                                                             cgcgACGTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCgg                  >  2:3211390/1‑99 (MQ=255)
                                                                               cgACGTCACCGCCGGGGAGATTCAGTCGGATATCAACTGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGt                >  1:3990439/1‑99 (MQ=255)
                                                                                   gTCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTAt            <  2:1494748/99‑1 (MQ=255)
                                                                                    tCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATg           <  1:1726116/99‑1 (MQ=255)
                                                                                    tCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATg           <  1:2395089/99‑1 (MQ=255)
                                                                                    tCACCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGGTGTAATTTCCGCAATTTAATAAGGCCCGGTAGTCATGTTTTCGGGTTTATg           >  2:2902957/1‑99 (MQ=255)
                                                                                      aCCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGt         >  2:2624688/1‑99 (MQ=255)
                                                                                      aCCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGt         >  2:74094/1‑99 (MQ=255)
                                                                                      aCCGCCGGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGt         <  2:82095/99‑1 (MQ=255)
                                                                                        cgccgGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGttt       <  2:4135140/99‑1 (MQ=255)
                                                                                        cgccgGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGttt       <  1:1156031/99‑1 (MQ=255)
                                                                                         gccgGGGAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTc      <  2:2293239/99‑1 (MQ=255)
                                                                                            ggggAGCTTCAGGCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTaa   <  2:1589664/99‑1 (MQ=255)
                                                                                            ggggAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTaa   <  1:3849294/99‑1 (MQ=255)
                                                                                             gggAGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTATTCATGTTTTCGGGTTTATGGTTTCTAAt  <  2:2748704/99‑1 (MQ=255)
                                                                                                |                                                                                               
CGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAAT  >  NC_000913/4132131‑4132322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A