Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,263	A→C	E162A (GAA→GCA)	yadG →	putative ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,263	0	A	C	96.4%	574.6 / ‑0.4	170	E162A (GAA→GCA)	yadG	putative ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (73/89); total (78/92)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

CGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACCACAC  >  NC_000913/143166‑143358

 gCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAacc                                                                                              <  2:1053657/100‑1 (MQ=255)

    acgaacgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccgac                                                                                           >  1:4056542/1‑100 (MQ=255)

    acgaacgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccgac                                                                                           <  1:658918/100‑1 (MQ=255)

     cgaacgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccgacc                                                                                          >  2:1720688/1‑100 (MQ=255)

      gaacgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccgaccg                                                                                         >  2:5137178/1‑100 (MQ=255)

      gaacgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccgaccg                                                                                         >  1:4521566/1‑100 (MQ=255)

         cgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAg                                                                                      <  1:4273527/100‑1 (MQ=255)

         cgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAg                                                                                      <  2:3504382/100‑1 (MQ=255)

          gTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAgg                                                                                     <  2:166965/100‑1 (MQ=255)

            gcgcGTATGTTATCTGGCGGGAGGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCg                                                                                   <  2:3178215/100‑1 (MQ=255)

               cgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTgg                                                                                <  1:1234715/100‑1 (MQ=255)

               cgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTgg                                                                                <  2:4458690/100‑1 (MQ=255)

               cgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATCCTCGACGCACCGACCGCAGGCGTgg                                                                                <  2:4378691/100‑1 (MQ=255)

                gTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGa                                                                               <  1:4507120/100‑1 (MQ=255)

                 tATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGTACCGACCGCAGGCGTGGat                                                                              <  2:5027844/100‑1 (MQ=255)

                 tATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGat                                                                              <  1:845517/100‑1 (MQ=255)

                 tATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGat                                                                              <  1:4478068/100‑1 (MQ=255)

                   tGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGatat                                                                            >  2:497314/1‑100 (MQ=255)

                    gTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATAtt                                                                           <  2:3279710/100‑1 (MQ=255)

                     ttATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTg                                                                          <  2:4133492/100‑1 (MQ=255)

                      tATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGa                                                                         <  1:4968480/100‑1 (MQ=255)

                      tATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGa                                                                         <  1:898070/100‑1 (MQ=255)

                      tATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGa                                                                         <  2:267249/100‑1 (MQ=255)

                       aTCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGaa                                                                        >  1:121862/1‑100 (MQ=255)

                       aTCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGaa                                                                        >  1:2368514/1‑100 (MQ=255)

                        tCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAAc                                                                       <  1:4195360/100‑1 (MQ=255)

                        tCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAAc                                                                       <  1:1778949/100‑1 (MQ=255)

                         cTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACt                                                                      <  2:5419417/100‑1 (MQ=255)

                         cTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGGGGATATTGAACt                                                                      >  2:1205532/1‑100 (MQ=255)

                          tGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACtt                                                                     >  1:5226422/1‑100 (MQ=255)

                           ggCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTc                                                                    >  1:2247571/1‑100 (MQ=255)

                           ggCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTc                                                                    >  1:4310410/1‑100 (MQ=255)

                             cGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgc                                                                  <  2:1833543/100‑1 (MQ=255)

                               ggATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgccg                                                                <  1:3072979/100‑1 (MQ=255)

                               ggATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgccg                                                                <  1:3072459/100‑1 (MQ=255)

                               ggATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgccg                                                                <  1:2474933/100‑1 (MQ=255)

                                gATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGCGGATATTGAACTTcgccgc                                                               >  2:3704070/1‑100 (MQ=255)

                                  tGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTc                                                             <  2:2842119/100‑1 (MQ=255)

                                   gAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCa                                                            <  2:2216130/100‑1 (MQ=255)

                                    aaGCGCCGTTTAATGATTGCCCGTGTGTTAATGCATGAACCTAACCTACTTATTCTCGACGAACCGACCGCAGGCGTGGCGATTGAACTTCGGCGCTTaa                                                           >  2:268424/1‑100 (MQ=255)

                                          cGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTggg                                                     <  1:2637872/100‑1 (MQ=255)

                                          cGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTggg                                                     <  2:3018979/100‑1 (MQ=255)

                                           gTTTAATGATTGCCCGTGCGTTAATACATGAACCTAAACTACTGAATCTCGACGTACCGACCGCCGGCGGCGATATTGAACTTCGCCGCTCAATGTgggg                                                    >  2:1118148/1‑100 (MQ=255)

                                            tttAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGc                                                   >  1:1201582/1‑100 (MQ=255)

                                              tAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCtt                                                 >  1:5314346/1‑100 (MQ=255)

                                                aTGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCtttt                                               <  1:1015706/100‑1 (MQ=255)

                                                aTGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCtttt                                               >  2:3969089/1‑100 (MQ=255)

                                                aTGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCtttt                                               <  1:1015686/100‑1 (MQ=255)

                                                 tGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCttttt                                              <  1:4941074/100‑1 (MQ=255)

                                                 tGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCttttt                                              <  1:4122781/100‑1 (MQ=255)

                                                    ttGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGaa                                           <  2:4022830/100‑1 (MQ=255)

                                                     tGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAg                                          <  1:206646/100‑1 (MQ=255)

                                                     tGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAg                                          >  1:2703851/1‑100 (MQ=255)

                                                      gCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAgg                                         >  1:696160/1‑100 (MQ=255)

                                                      gCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAgg                                         <  2:3609239/100‑1 (MQ=255)

                                                      gCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAgg                                         >  2:592285/1‑100 (MQ=255)

                                                       cccGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGa                                        >  2:4213382/1‑100 (MQ=255)

                                                       cccGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGa                                        <  1:2800670/100‑1 (MQ=255)

                                                       cccGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGa                                        <  1:599416/100‑1 (MQ=255)

                                                         cGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGAtt                                      >  2:2225226/1‑100 (MQ=255)

                                                         cGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGAtt                                      <  1:4698320/100‑1 (MQ=255)

                                                             cGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTaaa                                  >  1:3388019/1‑100 (MQ=255)

                                                              gTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAAc                                 <  1:3880567/100‑1 (MQ=255)

                                                              gTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAAc                                 <  2:4038149/100‑1 (MQ=255)

                                                              gTTAATGCAAGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAAc                                 >  1:5427910/1‑100 (MQ=255)

                                                               ttAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACg                                <  1:1805950/100‑1 (MQ=255)

                                                               ttAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACg                                <  2:2555274/100‑1 (MQ=255)

                                                               ttAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACg                                <  2:1895319/100‑1 (MQ=255)

                                                                 aaTGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGAc                              >  1:5136351/1‑100 (MQ=255)

                                                                    gCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACaaa                           >  2:467265/1‑100 (MQ=255)

                                                                    gCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACaaa                           >  1:5004879/1‑100 (MQ=255)

                                                                     cATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAg                          >  1:3938909/1‑100 (MQ=255)

                                                                         aaCCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcac                      <  2:4621617/100‑1 (MQ=255)

                                                                         aaCCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcac                      >  1:286350/1‑100 (MQ=255)

                                                                          aCCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGTGCTTTTTGAAGGATTTAAACGACAAAGGcacc                     <  2:3844230/100‑1 (MQ=255)

                                                                          aCCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcacc                     >  2:155040/1‑100 (MQ=255)

                                                                           ccTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcacca                    >  2:3128163/1‑100 (MQ=255)

                                                                             tAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCaccacc                  >  2:3512137/1‑100 (MQ=255)

                                                                              aaaCTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCAccacca                 <  2:1613747/100‑1 (MQ=255)

                                                                               aaCTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACTACcat                <  2:4831890/100‑1 (MQ=255)

                                                                                 ctactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca              >  2:4489934/1‑100 (MQ=255)

                                                                                 ctactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGACGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca              >  2:5320080/1‑100 (MQ=255)

                                                                                 ctactGATTCTCGACGCAACGACCGCAGGCGTGGATATTGAACTTCGCTGCTCAATGTGGTGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca              <  2:4971016/100‑1 (MQ=255)

                                                                                 ccactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca              <  2:2202713/98‑1 (MQ=255)

                                                                                  tactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatcat             <  1:2171454/100‑1 (MQ=255)

                                                                                    ctGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtc           <  2:4869764/100‑1 (MQ=255)

                                                                                      gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtctc         >  1:2411419/1‑100 (MQ=255)

                                                                                      gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtctc         >  1:3502898/1‑100 (MQ=255)

                                                                                      gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtctc         <  1:3738415/100‑1 (MQ=255)

                                                                                       aTTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGGAGGATTTAAAGGACTAAGGCACCACCATCATTCTca        >  2:5197913/1‑100 (MQ=255)

                                                                                       aTTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTca        <  2:4270804/100‑1 (MQ=255)

                                                                                        ttCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcac       >  2:3124724/1‑100 (MQ=255)

                                                                                         tctcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcacc      <  1:1368192/100‑1 (MQ=255)

                                                                                         tctcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcacc      <  2:3724516/100‑1 (MQ=255)

                                                                                          ctcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcacca     >  2:2111853/1‑100 (MQ=255)

                                                                                          ctcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcacca     >  1:3526506/1‑100 (MQ=255)

                                                                                           tcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcaccac    <  1:1687630/100‑1 (MQ=255)

                                                                                             gacgcACCGACCTCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACcacac  >  1:4785735/1‑100 (MQ=255)

                                                                                             gacgcACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCCCcacac  >  2:2159315/1‑100 (MQ=255)

                                                                                             gacgcACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACcacac  <  1:3157400/100‑1 (MQ=255)

CGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACCACAC  >  NC_000913/143166‑143358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A