Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,263	A→C	E162A (GAA→GCA)	yadG →	putative ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,263	0	A	C	98.5%	429.5 / ‑2.9	129	E162A (GAA→GCA)	yadG	putative ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (60/67); total (60/69)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 132 total aligned reads displayed.

CAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACCACACA  >  NC_000913/143168‑143359

cAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccg                                                                                              <  2:490242/100‑1 (MQ=255)

   cgaacgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCaccgacc                                                                                           >  2:762991/1‑100 (MQ=255)

       cgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAg                                                                                       >  2:327712/1‑100 (MQ=255)

       cgTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAg                                                                                       >  2:1973625/1‑100 (MQ=255)

        gTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTCAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAgg                                                                                      <  2:615182/100‑1 (MQ=255)

         tGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTTCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGc                                                                                     >  2:207022/1‑100 (MQ=255)

         tGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGc                                                                                     >  1:1203547/1‑100 (MQ=255)

         tGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGc                                                                                     >  1:373862/1‑100 (MQ=255)

          gcgcGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCg                                                                                    <  2:686825/100‑1 (MQ=255)

          gcgcGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCg                                                                                    <  1:46256/100‑1 (MQ=255)

           cgcgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGt                                                                                   >  1:2198417/1‑100 (MQ=255)

            gcgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTg                                                                                  <  1:3567963/100‑1 (MQ=255)

             cgTATTGTAGCTGGCGTGATGAAGCGCTGTTTCATGATTGCCCGGGCGGTGATGCATGAGCCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTgg                                                                                 <  2:3274636/100‑1 (MQ=255)

             cgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTgg                                                                                 <  2:216932/100‑1 (MQ=255)

             cgTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTgg                                                                                 <  1:958254/100‑1 (MQ=255)

              gTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGa                                                                                <  2:2512067/100‑1 (MQ=255)

              gTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGa                                                                                <  1:3139015/100‑1 (MQ=255)

               tATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGat                                                                               >  1:1338394/1‑100 (MQ=255)

                 tGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGatat                                                                             <  1:3442814/100‑1 (MQ=255)

                 tGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGatat                                                                             >  2:2416800/1‑100 (MQ=255)

                  gTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATAtt                                                                            >  2:1842291/1‑100 (MQ=255)

                   ttATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTg                                                                           <  2:3912155/100‑1 (MQ=255)

                      tCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAAc                                                                        <  2:1988457/100‑1 (MQ=255)

                      tCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAAc                                                                        <  2:892393/100‑1 (MQ=255)

                       cTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCCACCGCAGGCGCGGATATTGAACt                                                                       >  2:3255667/1‑100 (MQ=255)

                         ggCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATACTCGACGCACCGACCGCAGGCGTGGATATTGAACTTc                                                                     >  2:3629048/1‑100 (MQ=255)

                         ggCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGGTAATGCATGAACCTAAACTACTGATTCGCGACGCACCGACCGCAGGCGTGGATATTGAACTTc                                                                     >  2:3849928/1‑100 (MQ=255)

                           cGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgc                                                                   <  2:1653199/100‑1 (MQ=255)

                            gggATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgcc                                                                  <  2:417803/100‑1 (MQ=255)

                            gggATGAAGCGCCGTTTAATGATGGCCCGTGCGTTACTGCATGAAGCTAAACTACTGATTCTCGACGCACCGACCGCAGGCATAGATATTGAAATTcgac                                                                  <  2:934422/100‑3 (MQ=255)

                              gATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTcgccgc                                                                <  2:1585634/100‑1 (MQ=255)

                                 gAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCCGGCGTGGATATTGAACTTCGCCGCTCa                                                             >  2:107992/1‑100 (MQ=255)

                                    gcgcCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAAtg                                                          <  2:1767159/100‑1 (MQ=255)

                                       ccGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTgg                                                       <  2:1495801/100‑1 (MQ=255)

                                        cGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTggg                                                      >  1:1689461/1‑100 (MQ=255)

                                        cGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTggg                                                      >  1:2414350/1‑100 (MQ=255)

                                         gTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTgggg                                                     >  1:2752412/1‑100 (MQ=255)

                                         gTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTgggg                                                     <  1:3856594/100‑1 (MQ=255)

                                         gTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTgggg                                                     <  2:1200013/100‑1 (MQ=255)

                                         gTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTgggg                                                     >  1:1549983/1‑100 (MQ=255)

                                            tAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTCGATATTGAACTTCGCCGCTCCATGTGGGGCtt                                                  >  2:2356934/1‑100 (MQ=255)

                                              aTGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCtttt                                                <  2:1778328/100‑1 (MQ=255)

                                                gATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTg                                              <  1:1281634/100‑1 (MQ=255)

                                                   tGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAg                                           <  1:1961838/100‑1 (MQ=255)

                                                    gCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAgg                                          <  1:3438140/100‑1 (MQ=255)

                                                     cccGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGa                                         <  2:227950/100‑1 (MQ=255)

                                                     cccGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCACAATGTGGGGCTTTTTGAAGGa                                         <  1:3039879/100‑1 (MQ=255)

                                                       cGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCGGCTCAATGTGGGGCTTTTTGAAGGAtt                                       >  1:2403372/1‑100 (MQ=255)

                                                       cGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGAtt                                       <  1:2681485/100‑1 (MQ=255)

                                                        gTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGAttt                                      >  2:218347/1‑100 (MQ=255)

                                                           cGTAAATGCATGAAACGAAACTACTGATGCTCGACGCTCCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAAAATATaaa                                   <  2:2367860/100‑1 (MQ=255)

                                                             ttAATGCATGAACCTAAACTGCTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACg                                 <  2:963399/100‑1 (MQ=255)

                                                             ttAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACg                                 >  2:3841748/1‑100 (MQ=255)

                                                             ttAATGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACg                                 <  1:1461380/100‑1 (MQ=255)

                                                               aaTGCATGAACCTAAACTACTGATTCTTGACGCACCGACCGCAGGCGTTGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGAc                               >  2:3978795/1‑100 (MQ=255)

                                                               aaTGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGAc                               <  1:3204258/100‑1 (MQ=255)

                                                               aaTGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGAc                               >  1:2430046/1‑100 (MQ=255)

                                                                aTGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACa                              >  1:715045/1‑100 (MQ=255)

                                                                aTGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACa                              >  2:497454/1‑100 (MQ=255)

                                                                 tGCATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACaa                             >  2:334655/1‑100 (MQ=255)

                                                                   cATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAg                           >  1:2532183/1‑100 (MQ=255)

                                                                   cATGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAg                           >  2:2810069/1‑100 (MQ=255)

                                                                    aTGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAgg                          >  1:836450/1‑100 (MQ=255)

                                                                    aTGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAgg                          <  2:2950398/100‑1 (MQ=255)

                                                                     tGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGc                         >  2:3374347/1‑100 (MQ=255)

                                                                     tGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGc                         >  2:2295439/1‑100 (MQ=255)

                                                                     tGAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGc                         <  1:221936/100‑1 (MQ=255)

                                                                      gAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGTATTTAAACGACAAAGGca                        >  2:1714368/1‑100 (MQ=255)

                                                                      gAACCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGca                        <  1:2205944/100‑1 (MQ=255)

                                                                       aaCCTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcac                       >  2:2010376/1‑100 (MQ=255)

                                                                         ccTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcacca                     >  1:468251/1‑100 (MQ=255)

                                                                          cTAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGcaccac                    >  1:785378/1‑100 (MQ=255)

                                                                           tAAACTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCaccacc                   >  1:2560816/1‑100 (MQ=255)

                                                                            aaaCTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCAccacca                  <  2:703170/100‑1 (MQ=255)

                                                                            aaaCTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCAccacca                  >  1:903017/1‑100 (MQ=255)

                                                                             aaCTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcat                 <  2:3632351/100‑1 (MQ=255)

                                                                             aaCTACTGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcat                 <  1:2649340/100‑1 (MQ=255)

                                                                              actactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGCACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatc                <  2:2280947/100‑1 (MQ=255)

                                                                              actactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatc                >  1:1078151/1‑100 (MQ=255)

                                                                               ctactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca               <  1:705418/100‑1 (MQ=255)

                                                                               ctactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca               >  2:1382035/1‑100 (MQ=255)

                                                                               ctactGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACcatca               <  2:833616/100‑1 (MQ=255)

                                                                                tactGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGCTTTAAACGACAAAGGCACCACcatcat              >  2:2307549/1‑100 (MQ=255)

                                                                                  ctGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtc            <  2:3692167/100‑1 (MQ=255)

                                                                                  ctGATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtc            <  2:3402720/100‑1 (MQ=255)

                                                                                    gATTCTCGACGCACCGACCGCAGGCGTGGATATTGACCATCTCCGCGCACTGCGGGGCTTCTTGAAGGATTTGAACGACAAAGGCAGCACCATCATtgtc          >  2:2781113/1‑100 (MQ=255)

                                                                                    gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtctc          >  2:3843607/1‑100 (MQ=255)

                                                                                    gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtctc          <  1:1784475/100‑1 (MQ=255)

                                                                                    gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtctc          >  2:2131279/1‑100 (MQ=255)

                                                                                    gATTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCCCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATtatc          >  2:1598732/1‑100 (MQ=255)

                                                                                     aTTCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTca         <  1:546463/100‑1 (MQ=255)

                                                                                      ttCTCGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcac        >  1:1011597/1‑100 (MQ=255)

                                                                                        ctcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcacca      >  1:763764/1‑100 (MQ=255)

                                                                                        ctcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcacca      >  2:471895/1‑100 (MQ=255)

                                                                                         tcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcaccac     <  1:3632487/100‑1 (MQ=255)

                                                                                         tcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcaccac     <  2:130619/100‑1 (MQ=255)

                                                                                         tcGACGCACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTcaccac     <  2:818871/100‑1 (MQ=255)

                                                                                           gacgcACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACcacac   >  2:3466861/1‑100 (MQ=255)

                                                                                           gacgcACCGACCGCAGGCGTAGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACcacac   >  1:3177367/1‑100 (MQ=255)

                                                                                            acgcACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACcacaca  >  1:3844883/1‑100 (MQ=255)

CAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTCGACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAGGATTTAAACGACAAAGGCACCACCATCATTCTCACCACACA  >  NC_000913/143168‑143359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A