Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 400,871 T→G E18A (GAA→GCA)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,8710TG99.6% 849.4 / ‑4.6 241E18A (GAA→GCA) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (111/129);  total (112/129)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 241 total aligned reads displayed.

GTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAC  >  NC_000913/400774‑400967
                                                                                                  |                                                                                                
gTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCa                                                                                                <  1:450614/100‑1 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               >  1:3120679/1‑100 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               <  1:2742537/100‑1 (MQ=255)
    cATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGtt                                                                                            >  1:3599321/1‑100 (MQ=255)
    cATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGtt                                                                                            >  1:2015913/1‑100 (MQ=255)
      ttGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTcc                                                                                          <  1:3483340/100‑1 (MQ=255)
        gCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGc                                                                                        <  1:113650/100‑1 (MQ=255)
        gCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGc                                                                                        >  2:4786169/1‑100 (MQ=255)
        gCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGc                                                                                        >  2:3042733/1‑100 (MQ=255)
        gCCCTTGTCTATCAATGCCCAGCAGCACAACGGCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTGTTGCGGATTGCAGAGTTACTGCATGTTCCGc                                                                                        >  2:4508980/1‑100 (MQ=255)
         cccTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCt                                                                                       >  1:580726/1‑100 (MQ=255)
           cTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGa                                                                                     >  2:5429786/1‑100 (MQ=255)
           cTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGa                                                                                     >  1:3704799/1‑100 (MQ=255)
             tGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtt                                                                                   >  2:941231/1‑100 (MQ=255)
             tGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtn                                                                                   <  1:4422681/100‑2 (MQ=255)
              gTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAttt                                                                                  >  1:392969/1‑100 (MQ=255)
               tttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTa                                                                                 >  1:4172835/1‑100 (MQ=255)
               cttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtttn                                                                                 <  2:5496081/99‑2 (MQ=255)
                ttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTac                                                                                >  2:10365/1‑100 (MQ=255)
                ttATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTac                                                                                >  2:2602705/1‑100 (MQ=255)
                 tATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTacc                                                                               <  2:2006827/100‑1 (MQ=255)
                 tATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTacc                                                                               >  2:5039373/1‑100 (MQ=255)
                 naTCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaag                                                                               >  1:5570281/2‑98 (MQ=255)
                   tCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccac                                                                             >  2:217442/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                            >  2:1539620/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                            <  2:90630/100‑1 (MQ=255)
                     aaTGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTAccacca                                                                           <  2:1098689/100‑1 (MQ=255)
                        gCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaa                                                                        >  2:3465241/1‑100 (MQ=255)
                         cccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCTATTTCTGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                       >  2:1347333/1‑100 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAAc                                                                      >  2:2657869/1‑100 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAACGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAAc                                                                      <  1:2643493/100‑1 (MQ=255)
                           cagcagCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGGTTTACCACCAAAAACg                                                                     >  2:442448/1‑100 (MQ=255)
                           cagcagCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACg                                                                     <  2:2434146/100‑1 (MQ=255)
                             gcagcaCAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAt                                                                   <  2:1936915/100‑1 (MQ=255)
                               agcaCAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTc                                                                 <  2:3578264/100‑1 (MQ=255)
                                gcaCAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTcc                                                                <  2:1813858/100‑1 (MQ=255)
                                gcaCAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTcc                                                                <  1:2339969/100‑1 (MQ=255)
                                  acaACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTa                                                              <  1:1943039/100‑1 (MQ=255)
                                  acaACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTa                                                              <  1:4829539/100‑1 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             >  2:2770330/1‑100 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             <  2:3351425/100‑1 (MQ=255)
                                    aaCGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGTAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                            <  1:1226415/100‑1 (MQ=255)
                                    aaCGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                            <  1:1018042/100‑1 (MQ=255)
                                      cGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCg                                                          <  1:2605079/100‑1 (MQ=255)
                                       gTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGc                                                         >  1:2912010/1‑100 (MQ=255)
                                         cGAAGCGACTTTTAGCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAg                                                       <  2:1478251/100‑1 (MQ=255)
                                               gACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGTATGATCCGCTGATTTACCACCAAAAACGATTTCTACCCGCAGTTTTTc                                                 <  1:4886763/100‑1 (MQ=255)
                                               gACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTc                                                 <  1:4718887/100‑1 (MQ=255)
                                                aCTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTcc                                                <  2:1291463/100‑1 (MQ=255)
                                                aCTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACC‑AAAACGATTCCTACCCGCAGTTTTTCCa                                               <  2:3554076/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  1:5257525/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  2:3542529/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  1:2461606/1‑100 (MQ=255)
                                                 cTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  1:2536801/1‑100 (MQ=255)
                                                 cTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  2:1415155/1‑100 (MQ=255)
                                                   tttATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATc                                             <  1:2373686/100‑1 (MQ=255)
                                                    ttATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCt                                            <  2:3233096/100‑1 (MQ=255)
                                                     tATCAATGGCATCGACAAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCt                                            <  2:4620064/100‑1 (MQ=255)
                                                     tATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCtt                                           <  1:4930957/100‑1 (MQ=255)
                                                       tCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaa                                         <  2:3670701/100‑1 (MQ=255)
                                                       tCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaa                                         <  1:2753764/100‑1 (MQ=255)
                                                         aaTGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaga                                       >  2:5353477/1‑98 (MQ=255)
                                                         aaTGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaa                                       >  1:5454658/1‑100 (MQ=255)
                                                         aaTGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaa                                       >  2:3149410/1‑100 (MQ=255)
                                                         aaTGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGATTTTCCATCTTaaaa                                       >  1:698739/1‑100 (MQ=255)
                                                          aTGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaaa                                      >  2:4283166/1‑100 (MQ=255)
                                                           tGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAc                                     <  1:4205141/100‑1 (MQ=255)
                                                            ggCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    <  1:678232/100‑1 (MQ=255)
                                                            ggCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    >  2:2300782/1‑100 (MQ=255)
                                                            ggCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACGGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    <  1:3386538/100‑1 (MQ=255)
                                                              cATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTa                                  >  1:3435586/1‑100 (MQ=255)
                                                               aTCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTAt                                 <  1:120102/100‑1 (MQ=255)
                                                                 cGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATcc                               <  1:4107430/100‑1 (MQ=255)
                                                                 cGAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATcc                               <  1:404707/100‑1 (MQ=255)
                                                                  gAC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATccc                              >  1:271366/1‑100 (MQ=255)
                                                                   aC‑AATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCg                             <  1:1407334/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  2:2163990/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  1:1775811/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  2:3783812/100‑1 (MQ=255)
                                                                       aTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCt                          <  1:2428526/100‑1 (MQ=255)
                                                                        tGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTa                         <  1:3060650/100‑1 (MQ=255)
                                                                          ttttttGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAac                       <  2:2054434/100‑1 (MQ=255)
                                                                               tGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACaaa                  >  1:508789/1‑100 (MQ=255)
                                                                                tctgATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAg                 <  2:3963599/97‑1 (MQ=255)
                                                                                gCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTGCTACCCGCAGTTTTTCCCTCTTAAAAACCTATCCCATCTAACACAAAg                 >  1:5303330/1‑100 (MQ=255)
                                                                                gCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAg                 <  2:623724/100‑1 (MQ=255)
                                                                                 ccGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGt                <  1:4002513/100‑1 (MQ=255)
                                                                                     ttGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCAt            <  1:3654470/100‑1 (MQ=255)
                                                                                     ttGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCAt            <  2:4243511/100‑1 (MQ=255)
                                                                                     ttGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCAt            <  1:3475737/100‑1 (MQ=255)
                                                                                      tGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATa           <  1:2797142/100‑1 (MQ=255)
                                                                                      tGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATa           <  2:2548869/100‑1 (MQ=255)
                                                                                       ncAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTNCCATCTCAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  1:103988/2‑100 (MQ=255)
                                                                                       gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTACAAACCTATCCCGTCTAACACAAAGTGCATAc          >  1:1585139/1‑100 (MQ=255)
                                                                                       gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  2:2902765/1‑100 (MQ=255)
                                                                                       gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  2:122034/1‑100 (MQ=255)
                                                                                        cAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACa         >  1:3312173/1‑100 (MQ=255)
                                                                                          gagaCACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACAtt       >  2:692521/1‑100 (MQ=255)
                                                                                           agaCACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTa      <  1:3280602/100‑1 (MQ=255)
                                                                                             acacTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc    <  1:3021437/100‑1 (MQ=255)
                                                                                                  |                                                                                                
GTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGAC‑AATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAC  >  NC_000913/400774‑400967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A