Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 400,871 T→G E18A (GAA→GCA)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,8710TG98.1% 535.5 / ‑0.4 158E18A (GAA→GCA) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (79/76);  total (81/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 158 total aligned reads displayed.

GTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAC  >  NC_000913/400774‑400967
                                                                                                 |                                                                                                
gTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCa                                                                                                <  1:2873332/100‑1 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               >  1:2513856/1‑100 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               >  1:1455763/1‑100 (MQ=255)
 tGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCAt                                                                                               >  2:1317904/1‑100 (MQ=255)
  gCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATg                                                                                              <  1:2724629/100‑1 (MQ=255)
  gCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATg                                                                                              <  1:1903423/100‑1 (MQ=255)
   ccATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGt                                                                                             >  1:3858297/1‑100 (MQ=255)
         cccTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCt                                                                                       >  2:3516930/1‑100 (MQ=255)
         cccTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCt                                                                                       >  2:3860318/1‑100 (MQ=255)
          ccTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTg                                                                                      >  1:3468634/1‑100 (MQ=255)
            ttGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAt                                                                                    <  1:896537/100‑1 (MQ=255)
             tGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGAtt                                                                                   >  1:472859/1‑100 (MQ=255)
                 tATCAATGCCCAGCAGCACAACGTCGAAGCGACATTTATCAATGGCATCGACAATGTTTTTTGCCGCTTGCAGAGACACTGCATGTTCCGCTGATTTacc                                                                               >  1:1621372/1‑100 (MQ=255)
                  aTCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccn                                                                              <  1:1229265/100‑2 (MQ=255)
                  aTCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTacca                                                                              >  1:2802603/1‑100 (MQ=255)
                   tCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccac                                                                             >  1:215702/1‑100 (MQ=255)
                   tCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccac                                                                             >  1:595199/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCCATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGATGATTTaccacc                                                                            >  2:1998013/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                            >  1:3992411/1‑100 (MQ=255)
                    cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTaccacc                                                                            >  1:777867/1‑100 (MQ=255)
                       tGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaa                                                                         >  2:2646711/1‑100 (MQ=255)
                        gCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaa                                                                        >  1:2921019/1‑100 (MQ=255)
                         cccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                       >  2:1782897/1‑100 (MQ=255)
                         cccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                       >  1:2809696/1‑100 (MQ=255)
                         cccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCaaaaa                                                                       >  1:1148623/1‑100 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAAc                                                                      >  1:2464501/1‑100 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAAc                                                                      <  1:2594321/100‑1 (MQ=255)
                          ccAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAAc                                                                      >  2:1977518/1‑100 (MQ=255)
                           cagcagCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACg                                                                     >  1:528627/1‑100 (MQ=255)
                           cagcagCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACg                                                                     <  2:1252913/100‑1 (MQ=255)
                              cagcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGAtt                                                                  >  2:1209727/1‑100 (MQ=255)
                                gcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTcc                                                                >  1:1050252/1‑100 (MQ=255)
                                gcaCAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTcc                                                                <  1:317634/100‑1 (MQ=255)
                                  acaACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTa                                                              >  1:3784061/1‑100 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGTCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             >  1:1212023/1‑100 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             >  2:1243820/1‑100 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             >  1:2016108/1‑100 (MQ=255)
                                   caACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAc                                                             <  1:2928068/100‑1 (MQ=255)
                                    aaCGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTAcc                                                            <  2:3948861/100‑1 (MQ=255)
                                       gTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGc                                                         >  1:2694135/1‑100 (MQ=255)
                                         cGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTTTTTTTTTTTTTAACACTGAATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAg                                                       >  2:3623566/1‑100 (MQ=255)
                                           aaGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGtt                                                     >  2:83419/1‑100 (MQ=255)
                                            aGCGACTTTTATCAATGGCTTCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATACCTACCCGCAGttt                                                    >  2:3651031/1‑100 (MQ=255)
                                             gCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCTATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGtttt                                                   <  2:3637968/100‑1 (MQ=255)
                                               gACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGtttttn                                                 <  1:1590319/100‑2 (MQ=255)
                                               gACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTc                                                 <  2:838523/100‑1 (MQ=255)
                                                aCTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTcc                                                <  2:2944866/100‑1 (MQ=255)
                                                aCTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTcc                                                <  1:1945410/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTccn                                               <  1:1018526/100‑2 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               >  2:1451721/1‑100 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  1:2388436/100‑1 (MQ=255)
                                                 cTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCa                                               <  1:641169/100‑1 (MQ=255)
                                                   tttATCAATAGCATCGACAATGTTTTTTGCCGATTGCAGAGATACTGCATGTTCTGCTGATTTACCACCGAAAACGATTCCTACGCGCAGTTTTTCCATc                                             <  1:3550521/100‑1 (MQ=255)
                                                    ttATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCt                                            <  1:980024/100‑1 (MQ=255)
                                                     tATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCtt                                           <  2:875874/100‑1 (MQ=255)
                                                       tCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaa                                         <  1:3811873/100‑1 (MQ=255)
                                                        cAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaa                                        >  1:2317066/1‑100 (MQ=255)
                                                        cAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaa                                        >  2:246465/1‑100 (MQ=255)
                                                        cAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTGACCACCAAAAACGATTCCTACCCGCAGTTTTTGCATCTTaaa                                        >  1:3551402/1‑100 (MQ=255)
                                                         aaTGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTaaaa                                       >  1:2895721/1‑100 (MQ=255)
                                                           tGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAc                                     <  1:1342157/100‑1 (MQ=255)
                                                           tGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAc                                     <  1:686049/100‑1 (MQ=255)
                                                            ggCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    <  1:1024391/100‑1 (MQ=255)
                                                            ggCATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAAcc                                    <  2:2637831/100‑1 (MQ=255)
                                                              cATCGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTa                                  >  2:2673631/1‑100 (MQ=255)
                                                              cATCAACAATGTTTTATGCCGATTGCAGAGACACTGCATGTTCTGCTGATTTACCAACAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTa                                  <  2:2466412/100‑1 (MQ=255)
                                                                 cGACAATGTTTTTTTTTGATTTCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATcc                               >  2:762956/1‑100 (MQ=255)
                                                                 cGACAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATcc                               >  1:1778600/1‑100 (MQ=255)
                                                                   aCAATGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCg                             >  1:2679463/1‑100 (MQ=255)
                                                                     aaTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTc                           <  2:1745829/100‑1 (MQ=255)
                                                                     aaTGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTc                           <  1:3443971/100‑1 (MQ=255)
                                                                       tGTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTa                         <  2:2095958/100‑1 (MQ=255)
                                                                        gTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTaa                        >  1:2489929/1‑100 (MQ=255)
                                                                        gTTTTTTGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTaa                        >  2:3240471/1‑100 (MQ=255)
                                                                         ttttttGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAac                       <  2:2075387/100‑1 (MQ=255)
                                                                         ttttttGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAac                       <  2:3526846/100‑1 (MQ=255)
                                                                         ttttttGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAac                       >  1:165175/1‑100 (MQ=255)
                                                                            tttGCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAAcaca                    <  1:1793317/100‑1 (MQ=255)
                                                                               gCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCTTCTAACACAAAg                 <  1:2196499/100‑1 (MQ=255)
                                                                               gCCGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACCCAAAg                 >  1:3067129/1‑100 (MQ=255)
                                                                                ccGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGt                <  1:682809/100‑1 (MQ=255)
                                                                                ccGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGt                >  2:2277791/1‑100 (MQ=255)
                                                                                ccGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACACGCAGTTTTCCTATCTTAAATACCCATCCCGTCTAACACAAAGt                <  2:694973/100‑1 (MQ=255)
                                                                                 cGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTg               <  1:191220/100‑1 (MQ=255)
                                                                                 cGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTg               >  2:3554708/1‑100 (MQ=255)
                                                                                 cGATTGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTg               >  2:1756870/1‑100 (MQ=255)
                                                                                     tGCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATa           >  1:1319384/1‑100 (MQ=255)
                                                                                      gCAGAGACACTGCATGTTCCGCTGATTTACTACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          <  2:1880106/100‑1 (MQ=255)
                                                                                      gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          <  2:1749702/100‑1 (MQ=255)
                                                                                      gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          >  2:1155156/1‑100 (MQ=255)
                                                                                      gCAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATAc          <  1:3690454/100‑1 (MQ=255)
                                                                                       cAGAGACACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACa         <  1:531469/100‑1 (MQ=255)
                                                                                         gagaCACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACAtt       >  2:474681/1‑100 (MQ=255)
                                                                                         gagaCACTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACAtt       <  1:3287741/100‑1 (MQ=255)
                                                                                            acacTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc    <  2:3070020/100‑1 (MQ=255)
                                                                                            acacTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc    <  1:1512105/100‑1 (MQ=255)
                                                                                            acacTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTAcc    <  2:163259/100‑1 (MQ=255)
                                                                                              acTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAc  >  2:3415770/1‑100 (MQ=255)
                                                                                              acTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAc  >  2:3182758/1‑100 (MQ=255)
                                                                                              acTGCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAc  >  1:3337070/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCAC  >  NC_000913/400774‑400967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A