Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 467,975 T→G N188K (AAT→AAG)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913467,9750TG98.7% 804.9 / ‑2.9 230N188K (AAT→AAG) cofthiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (118/108);  total (120/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

CACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTA  >  NC_000913/467878‑468071
                                                                                                 |                                                                                                
  cGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAg                                                                                              <  1:2863464/100‑1 (MQ=255)
    cTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGgc                                                                                            <  2:2102179/100‑1 (MQ=255)
      tGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGgcgc                                                                                          <  1:2315973/100‑1 (MQ=255)
        cAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTg                                                                                        <  2:2854872/100‑1 (MQ=255)
         aGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGc                                                                                       <  2:3514115/100‑1 (MQ=255)
          naTCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCa                                                                                      >  1:4443853/2‑100 (MQ=255)
          gATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCa                                                                                      >  1:465360/1‑100 (MQ=255)
          gATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCa                                                                                      <  1:1443853/100‑1 (MQ=255)
           acccAGCTTTAGCAAGCATGAGGCGAGCTGGCACGTTGGTGTTTTTCCGCGACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCAt                                                                                     <  2:2756222/98‑1 (MQ=255)
           aTCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCAt                                                                                     <  1:1330756/100‑1 (MQ=255)
             ccAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTg                                                                                   >  1:876953/1‑100 (MQ=255)
              cAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGa                                                                                  >  1:901448/1‑100 (MQ=255)
               aGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGAc                                                                                 >  1:4082113/1‑100 (MQ=255)
                gCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACg                                                                                >  2:3077873/1‑100 (MQ=255)
                 cTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACgg                                                                               <  1:1508941/100‑1 (MQ=255)
                  tataCGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGt                                                                              <  1:3542284/100‑1 (MQ=255)
                  tataCGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGt                                                                              >  2:2023883/1‑100 (MQ=255)
                    taCGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGGGCTGCATTGACGGTGc                                                                            <  2:5314776/100‑1 (MQ=255)
                    taCGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGc                                                                            >  1:5334292/1‑100 (MQ=255)
                      cGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTg                                                                          >  1:392012/1‑100 (MQ=255)
                      cGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTg                                                                          >  1:2797655/1‑100 (MQ=255)
                       gAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGa                                                                         >  1:2088972/1‑100 (MQ=255)
                       gAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGa                                                                         >  1:114414/1‑100 (MQ=255)
                        aaGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGAc                                                                        >  1:2447559/1‑100 (MQ=255)
                         aGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCGAGAAAGGCGCTGCATTGACGGTGCTGAcc                                                                       <  2:2525380/100‑1 (MQ=255)
                         aGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGAcc                                                                       <  1:4550874/100‑1 (MQ=255)
                         aGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGAcc                                                                       <  2:1655435/100‑1 (MQ=255)
                           cATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCa                                                                     >  2:779909/1‑100 (MQ=255)
                           cATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCa                                                                     >  2:1086218/1‑100 (MQ=255)
                           cATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCa                                                                     >  2:4785236/1‑100 (MQ=255)
                           cATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAAGCGCTGCATTGACGGTGCTGACCCa                                                                     >  2:1162723/1‑100 (MQ=255)
                              tAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACa                                                                  <  1:2019461/100‑1 (MQ=255)
                               aGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACAt                                                                 <  1:5067320/100‑1 (MQ=255)
                                ggCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACAtt                                                                >  1:3597844/1‑100 (MQ=255)
                                 gCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACAttt                                                               <  2:5095782/100‑1 (MQ=255)
                                    aGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAgg                                                            <  1:2190070/100‑1 (MQ=255)
                                    aGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAgg                                                            <  1:4888807/100‑1 (MQ=255)
                                    aGCGTGCACATTTGTGTTTTTCCGCCACGGAGTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAgg                                                            <  1:574954/100‑1 (MQ=255)
                                     gCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGt                                                           <  2:1473669/100‑1 (MQ=255)
                                     gCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGt                                                           <  2:3564617/100‑1 (MQ=255)
                                      cGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGtt                                                          >  2:2454428/1‑100 (MQ=255)
                                       gTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGttt                                                         <  2:1374517/100‑1 (MQ=255)
                                       gTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGttt                                                         >  1:1141008/1‑100 (MQ=255)
                                        tGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTa                                                        <  2:5344567/100‑1 (MQ=255)
                                          cacaTTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATc                                                      >  1:2491347/1‑100 (MQ=255)
                                          cacaTTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATc                                                      >  1:319550/1‑100 (MQ=255)
                                          cacaTTTGTGGTTTTCCGCCACGGATTGCCTCGAAGGGCGGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATc                                                      <  2:237943/100‑1 (MQ=255)
                                           acaTTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCg                                                     <  1:4756429/100‑1 (MQ=255)
                                             aTTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGtt                                                   >  2:2622703/1‑100 (MQ=255)
                                              tttGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTg                                                  <  2:2126434/100‑1 (MQ=255)
                                               ttGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTgc                                                 >  1:3432853/1‑100 (MQ=255)
                                                tgtgTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTgcg                                                >  2:4424491/1‑100 (MQ=255)
                                                    tttttCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGAt                                            >  1:2392941/1‑100 (MQ=255)
                                                      tttCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTg                                          <  1:2881566/100‑1 (MQ=255)
                                                      tttCCGCCACGCATTGCCTCGAAGTGCTGCCGGTGGGCTGTAAGAAAGGCGCTGCATTGACGGTGCTGACCTTACATTTAGGTTTATCGTTGCGCGATTg                                          <  1:4906802/100‑1 (MQ=255)
                                                         ccGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAt                                       <  1:3507639/100‑1 (MQ=255)
                                                          cGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATg                                      <  2:88963/100‑1 (MQ=255)
                                                          cGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATg                                      >  2:1585470/1‑100 (MQ=255)
                                                          cGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATg                                      >  2:1239680/1‑100 (MQ=255)
                                                          cGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATg                                      >  2:3621531/1‑100 (MQ=255)
                                                           gCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAACGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATgg                                     >  1:5290982/1‑100 (MQ=255)
                                                           gCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATgg                                     >  1:3016396/1‑100 (MQ=255)
                                                           gCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATgg                                     >  2:2152020/1‑100 (MQ=255)
                                                           gCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATgg                                     >  2:2233998/1‑100 (MQ=255)
                                                           gCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGCGCTGACCCAACATTTAGGTTTATCGTCGCGCGATTGCATgg                                     >  2:4557869/1‑100 (MQ=255)
                                                              aCGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGCAAGGCGCTGCCTTGACGGTGCTGACTCCAAAATTAGGATTATTGTTGCGAGATTGCATGGACt                                  >  2:4149243/1‑100 (MQ=255)
                                                              aCGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCt                                  <  1:1862616/100‑1 (MQ=255)
                                                              aCGGATTGCCTCGAAGTGCTGCCGGTGGGCTGAAAGAAAGGCGCTGCATTGCCGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCt                                  <  1:3341611/100‑1 (MQ=255)
                                                                  aTTGCCTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGTATTGACGGTGCTGCCCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTgg                              <  2:3593688/100‑1 (MQ=255)
                                                                     gCCTCGAAGAGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGa                           >  1:5431621/1‑100 (MQ=255)
                                                                      ccTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGAt                          >  2:1813036/1‑100 (MQ=255)
                                                                       ctctAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATg                         >  1:4286367/1‑100 (MQ=255)
                                                                       cTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAGCATTTAGGTTTATCGCTGCGCGATTGCATGGCCTTTGGTGATg                         >  1:699346/1‑100 (MQ=255)
                                                                       cTCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATg                         >  2:337713/1‑100 (MQ=255)
                                                                        tCGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGc                        <  1:641404/100‑1 (MQ=255)
                                                                         cGAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCg                       >  2:3888955/1‑100 (MQ=255)
                                                                          gAAGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGa                      >  2:3235109/1‑100 (MQ=255)
                                                                            aGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATg                    >  1:2417208/1‑100 (MQ=255)
                                                                            aGTGCTGCCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATg                    >  1:1770680/1‑100 (MQ=255)
                                                                              tgctgcCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGaa                  >  1:2763223/1‑100 (MQ=255)
                                                                               gctgcCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAAc                 >  1:2990330/1‑100 (MQ=255)
                                                                               gctgcCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAAc                 >  1:3837583/1‑100 (MQ=255)
                                                                               gctgcCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAAc                 <  2:3473851/100‑1 (MQ=255)
                                                                               gctgcCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAAc                 <  2:316562/100‑1 (MQ=255)
                                                                                ctgcCGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGGTGAACg                >  1:660023/1‑100 (MQ=255)
                                                                                   ccGGTGGGCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATc             >  1:1690652/1‑100 (MQ=255)
                                                                                    cGGTGGGCTCCGAGAAAGGCGCAACATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATcg            >  2:687634/1‑100 (MQ=255)
                                                                                      gTGGGCTGCTAGAAAGGCGCTGCGTTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGTGCGATTGCATGGCCTTTGGTGATGCGATGAACGATcgcg          <  1:4853768/100‑1 (MQ=255)
                                                                                         ggCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGaaa       <  2:2592435/100‑1 (MQ=255)
                                                                                          gCTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGaaan      <  1:2059628/100‑2 (MQ=255)
                                                                                           cTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATg     >  1:1001224/1‑100 (MQ=255)
                                                                                           cTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATg     <  1:1635386/100‑1 (MQ=255)
                                                                                           cTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATg     >  1:4404836/1‑100 (MQ=255)
                                                                                           cTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATg     >  1:1906888/1‑100 (MQ=255)
                                                                                           cTGCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATg     >  2:2687790/1‑100 (MQ=255)
                                                                                             gCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGCAATGtt   >  2:4220677/1‑100 (MQ=255)
                                                                                             gCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGtt   <  2:4045123/100‑1 (MQ=255)
                                                                                             gCAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGtt   >  1:3372082/1‑100 (MQ=255)
                                                                                              cAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTa  >  2:4278934/1‑100 (MQ=255)
                                                                                              cAAGAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTa  >  1:3105594/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTA  >  NC_000913/467878‑468071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A