Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 615,736 A→C E527A (GAA→GCA)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913615,7360AC99.4% 627.4 / ‑4.2 175E527A (GAA→GCA) entFenterobactin synthase multienzyme complex component, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (75/99);  total (75/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 175 total aligned reads displayed.

AATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCA  >  NC_000913/615639‑615831
                                                                                                 |                                                                                               
aaTCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAg                                                                                               <  2:3996250/100‑1 (MQ=255)
 aTCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGc                                                                                              <  1:4912533/100‑1 (MQ=255)
  tCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCt                                                                                             <  1:4185691/100‑1 (MQ=255)
   ctgctgCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTg                                                                                            <  1:2929608/100‑1 (MQ=255)
    tgctgcGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTgg                                                                                           >  2:604572/1‑100 (MQ=255)
    tgctgcGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTgg                                                                                           >  1:528173/1‑100 (MQ=255)
     gctgcGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGa                                                                                          >  2:1298270/1‑100 (MQ=255)
       tgcGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGc                                                                                        >  1:1141282/1‑100 (MQ=255)
       tgcGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGc                                                                                        <  2:4111108/100‑1 (MQ=255)
           tGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGcc                                                                                    <  2:4823144/100‑1 (MQ=255)
            gAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCt                                                                                   >  2:1035684/1‑100 (MQ=255)
            gAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCt                                                                                   <  2:2154344/100‑1 (MQ=255)
             aGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTg                                                                                  <  1:2467555/100‑1 (MQ=255)
                 cggcgTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTa                                                                              >  2:5346944/1‑100 (MQ=255)
                    cgTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCCTGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCg                                                                           >  2:1011572/1‑100 (MQ=255)
                     gTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGc                                                                          >  1:4715788/1‑100 (MQ=255)
                       tAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTg                                                                        <  1:2247278/100‑1 (MQ=255)
                       tAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTg                                                                        <  2:2456986/100‑1 (MQ=255)
                        aaaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGCCCCTGGCACTCCATGCGATAGTCGCAGCTGGAGCGGCCTGGCTACCGCTgg                                                                       <  1:2139238/100‑1 (MQ=255)
                        aaaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTgg                                                                       <  2:687147/100‑1 (MQ=255)
                        aaaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTgg                                                                       <  2:2766518/100‑1 (MQ=255)
                        aaaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTgg                                                                       <  1:43702/100‑1 (MQ=255)
                        aaaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTgg                                                                       <  1:3417044/100‑1 (MQ=255)
                        aaaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTgg                                                                       <  1:52148/100‑1 (MQ=255)
                         aaCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGa                                                                      <  2:324829/100‑1 (MQ=255)
                          aCCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGAt                                                                     <  1:3432242/100‑1 (MQ=255)
                           ccAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATa                                                                    >  1:4895685/1‑100 (MQ=255)
                            cAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATAc                                                                   <  1:1389071/100‑1 (MQ=255)
                             aGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATAcc                                                                  >  1:1256066/1‑100 (MQ=255)
                             aGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATAcc                                                                  <  1:2700269/100‑1 (MQ=255)
                             aGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTGGCAGCTGGAGCGGCCTGGCTACCGCTGGATAcc                                                                  <  2:2939564/100‑1 (MQ=255)
                               gggACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCgg                                                                >  1:3104414/1‑100 (MQ=255)
                                ggACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGc                                                               <  1:1000059/100‑1 (MQ=255)
                                ggACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGc                                                               <  1:5534309/100‑1 (MQ=255)
                                 gACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCt                                                              >  2:3824408/1‑100 (MQ=255)
                                  aCAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTa                                                             <  2:4927267/100‑1 (MQ=255)
                                   cAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTAt                                                            >  2:4232518/1‑100 (MQ=255)
                                       gTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCgg                                                        >  1:3564258/1‑100 (MQ=255)
                                       gTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCgg                                                        <  1:5038528/100‑1 (MQ=255)
                                          gCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACg                                                     <  2:1695720/100‑1 (MQ=255)
                                           cGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGa                                                    >  2:4081933/1‑100 (MQ=255)
                                            ggTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGAt                                                   <  2:1718198/100‑1 (MQ=255)
                                              tGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCg                                                 >  1:4696274/1‑100 (MQ=255)
                                              tGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCg                                                 >  1:1746345/1‑100 (MQ=255)
                                                gCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGcc                                               <  1:2344572/100‑1 (MQ=255)
                                                gCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGcc                                               <  1:2877584/100‑1 (MQ=255)
                                                 cACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCt                                              >  2:207886/1‑100 (MQ=255)
                                                 cACTACCGCGCTCGGTCTTTTTGACCCTCGCACTCCACGCGATAGTTGCAGCTGGAGCGCCCTTGCTACCGCTGCATACCGGCTATCCGGACGATCTCCt                                              <  2:2416996/100‑1 (MQ=255)
                                                   cTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGa                                            >  1:3823058/1‑100 (MQ=255)
                                                   cTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGa                                            >  1:2994668/1‑100 (MQ=255)
                                                    tACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGaa                                           >  2:4041089/1‑100 (MQ=255)
                                                    tACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGaa                                           <  2:4577664/100‑1 (MQ=255)
                                                     aCCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGaaa                                          <  1:4829307/100‑1 (MQ=255)
                                                      ccGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGaaaa                                         <  2:2001095/100‑1 (MQ=255)
                                                        gcgcTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAatg                                       <  1:1315660/100‑1 (MQ=255)
                                                         cgcTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAatga                                      <  1:3193970/100‑1 (MQ=255)
                                                          gcTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAatgat                                     <  1:3155137/100‑1 (MQ=255)
                                                          gcTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAatgat                                     <  2:1318028/100‑1 (MQ=255)
                                                            tCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGc                                   <  1:4626986/100‑1 (MQ=255)
                                                            tCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGc                                   <  2:5094749/100‑1 (MQ=255)
                                                              ggTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTg                                 <  1:1084637/100‑1 (MQ=255)
                                                              ggTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTg                                 <  1:614781/100‑1 (MQ=255)
                                                               gTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTgg                                >  2:2708908/1‑100 (MQ=255)
                                                               gTCTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTgg                                >  1:1945024/1‑100 (MQ=255)
                                                                 cTTTTTGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGaa                              >  2:2407556/1‑100 (MQ=255)
                                                                  tttttGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAg                             <  2:1573559/100‑1 (MQ=255)
                                                                  tttttGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAg                             <  1:3524734/100‑1 (MQ=255)
                                                                   ttttGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGa                            <  2:4891657/100‑1 (MQ=255)
                                                                   ttttGACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGa                            >  1:305548/1‑100 (MQ=255)
                                                                       gACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATgcg                        <  1:3690257/100‑1 (MQ=255)
                                                                       gACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATgcg                        <  2:1805515/100‑1 (MQ=255)
                                                                       gACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATgcg                        <  1:1835403/100‑1 (MQ=255)
                                                                       gACCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATgcg                        <  2:3020980/100‑1 (MQ=255)
                                                                        aCCCTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATAACGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATgcgc                       >  2:2846852/1‑100 (MQ=255)
                                                                         cccTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGcgcg                      >  1:3774707/1‑100 (MQ=255)
                                                                         cccTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGcgcg                      <  1:4271596/100‑1 (MQ=255)
                                                                         cccTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGcgcg                      <  1:3110465/100‑1 (MQ=255)
                                                                           ttGGCTCTCCATGCGCTAGTTGCAGCTGGAGCGGTGTGGCTACCTCTGGATACCGGCTTTCCGGACGATGGCATGAAAATGATGCTGGAAGATGCGcgtc                    <  2:5241094/99‑1 (MQ=255)
                                                                           cTGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGcgtc                    >  1:2168736/1‑100 (MQ=255)
                                                                            tGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGcgtcc                   >  2:571168/1‑100 (MQ=255)
                                                                            tGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGcgtcc                   >  2:3762660/1‑100 (MQ=255)
                                                                            tGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGcgtcc                   <  2:3510957/100‑1 (MQ=255)
                                                                            tGGCACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGcgtcc                   <  2:1666168/100‑1 (MQ=255)
                                                                               cACTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGcgtccgtc                >  2:1403359/1‑100 (MQ=255)
                                                                                aCTCCATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCg               >  1:2856844/1‑100 (MQ=255)
                                                                                   ccATGCGATAGTTGCAGCTGGAGTGGCCTGGCTACCGCTGGATACCGGCTACCCGGACGATCGCCTGAACATGATGCTGGAAGATGCGCGTCCGTCGCTg            >  1:5305285/1‑100 (MQ=255)
                                                                                    cATGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCCCTGt           <  2:4643807/100‑1 (MQ=255)
                                                                                     aTGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTgat          >  1:1863294/1‑98 (MQ=255)
                                                                                      tGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTa         <  2:44728/100‑1 (MQ=255)
                                                                                      tGCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTa         >  1:4176894/1‑100 (MQ=255)
                                                                                      ggCGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTa         <  2:4145869/99‑1 (MQ=255)
                                                                                        cGATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAAt       <  1:1652923/100‑1 (MQ=255)
                                                                                        cGATAGTGGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAAt       <  2:3233951/100‑1 (MQ=255)
                                                                                         gATAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAAtt      <  2:1881332/100‑1 (MQ=255)
                                                                                          aTAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTACCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAAttn     <  1:2267791/100‑2 (MQ=255)
                                                                                           tAGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTac    <  2:3419395/100‑1 (MQ=255)
                                                                                            aGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGACGATGCGCGTCCGTCGCTGTTAATTacc   >  2:2012413/1‑100 (MQ=255)
                                                                                            aGTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTacc   <  1:3913799/100‑1 (MQ=255)
                                                                                             gTTGCAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTacca  >  2:150889/1‑100 (MQ=255)
                                                                                             gTTGCAGCTGGAGCGGCCTGGCTACCGCGGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTacca  <  1:1525092/100‑1 (MQ=255)
                                                                                                 |                                                                                               
AATCTGCTGCGTGAGCGCGGCGTTAAACCAGGGGACAGCGTGGCGGTGGCACTACCGCGCTCGGTCTTTTTGACCCTGGCACTCCATGCGATAGTTGAAGCTGGAGCGGCCTGGCTACCGCTGGATACCGGCTATCCGGACGATCGCCTGAAAATGATGCTGGAAGATGCGCGTCCGTCGCTGTTAATTACCA  >  NC_000913/615639‑615831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A