Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,422	(C)_8→7	coding (903/1872 nt)	gsiA →	glutathione ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,415	0	C	.	97.9%	897.1 / 8.9	192	coding (896/1872 nt)	gsiA	glutathione ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (2/2); new base . (102/86); total (104/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

GTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAAT  >  NC_000913/868316‑868512

  gcgcTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGccc                                                                                                 <  1:1826005/100‑1 (MQ=255)

       gTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGccccccca                                                                                            >  1:1009472/1‑99 (MQ=255)

         tAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGcccccccatc                                                                                          >  1:3152676/1‑97 (MQ=255)

         tAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGGTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGcccccccatc                                                                                          >  1:3208884/1‑97 (MQ=255)

           gctgctGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGa                                                                                       >  2:880766/1‑100 (MQ=255)

           gctgctGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGa                                                                                       >  1:23816/1‑100 (MQ=255)

            ctgctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAg                                                                                      <  2:2034044/100‑1 (MQ=255)

             tgctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCGCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAga                                                                                     >  1:4737205/1‑99 (MQ=255)

                 tttCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaa                                                                                 <  1:2915800/99‑1 (MQ=255)

                 gTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGag                                                                                 >  2:3355640/1‑99 (MQ=255)

                 gTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaa                                                                                 >  2:103274/1‑100 (MQ=255)

                  ttCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGaaa                                                                                >  1:2861144/1‑100 (MQ=255)

                    ccGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAAc                                                                              >  2:1825088/1‑100 (MQ=255)

                     cGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACg                                                                             >  2:156019/1‑100 (MQ=255)

                     cGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACg                                                                             >  2:431971/1‑100 (MQ=255)

                      gCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATGTCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACgg                                                                            >  2:4980202/1‑100 (MQ=255)

                          cTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACggtggt                                                                        <  1:5461061/100‑1 (MQ=255)

                             ggTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGa                                                                     <  1:3915734/100‑1 (MQ=255)

                               tGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATg                                                                   >  2:3363477/1‑100 (MQ=255)

                               tGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATg                                                                   <  1:4032705/100‑1 (MQ=255)

                               tGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATg                                                                   <  1:3477867/100‑1 (MQ=255)

                                gCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATgg                                                                  >  2:1549531/1‑100 (MQ=255)

                                gCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATgg                                                                  >  1:1294066/1‑100 (MQ=255)

                                 cGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGc                                                                 >  2:1280912/1‑100 (MQ=255)

                                 cGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGc                                                                 <  1:3404110/100‑1 (MQ=255)

                                  gATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCTCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                                <  2:4947763/100‑1 (MQ=255)

                                  gATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                                <  2:5374533/100‑1 (MQ=255)

                                    tGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGaa                                                              >  1:1173036/1‑100 (MQ=255)

                                     gAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAc                                                             <  1:669377/100‑1 (MQ=255)

                                      aaaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                            <  1:3613883/100‑1 (MQ=255)

                                      aaaGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                            <  1:3631074/100‑1 (MQ=255)

                                        aGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTg                                                          >  1:5250873/1‑100 (MQ=255)

                                          ggTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                                                        >  2:1671453/1‑100 (MQ=255)

                                          ggTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                                                        >  2:1519112/1‑100 (MQ=255)

                                          ggTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGtt                                                        >  1:2224182/1‑100 (MQ=255)

                                           gTTAGATTATCCCCGATGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGttt                                                       <  1:4590530/100‑1 (MQ=255)

                                           gTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGttt                                                       >  1:3671085/1‑100 (MQ=255)

                                           gTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGttt                                                       >  1:1468170/1‑100 (MQ=255)

                                              aGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGGACCTGTTTTAc                                                    >  1:488243/1‑100 (MQ=255)

                                              aGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                                                    >  1:5270436/1‑100 (MQ=255)

                                                aTTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                                  >  2:211161/1‑100 (MQ=255)

                                                aTTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGa                                                  <  1:1333408/100‑1 (MQ=255)

                                                 ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGTAAACGGTGGTGGATGGCGAACCTGTTTTACGAg                                                 >  2:811912/1‑100 (MQ=255)

                                                 ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAg                                                 <  1:323793/100‑1 (MQ=255)

                                                 ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAg                                                 >  1:4612836/1‑100 (MQ=255)

                                                 ttATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAg                                                 >  2:4885389/1‑100 (MQ=255)

                                                   aTCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTg                                               <  1:1559506/100‑1 (MQ=255)

                                                   aTCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTg                                               <  1:2631964/100‑1 (MQ=255)

                                                      cccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGt                                            <  2:3261866/100‑1 (MQ=255)

                                                      cccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGt                                            >  1:4107269/1‑100 (MQ=255)

                                                        cGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTaa                                          <  1:5104276/100‑1 (MQ=255)

                                                           cGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCt                                       <  2:383119/100‑1 (MQ=255)

                                                            gTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCtt                                      <  2:2342690/100‑1 (MQ=255)

                                                            gTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCtt                                      >  2:1593408/1‑100 (MQ=255)

                                                             tttCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTg                                     <  2:3053308/100‑1 (MQ=255)

                                                             tttCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTg                                     <  2:1456829/100‑1 (MQ=255)

                                                              ttCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGt                                    >  2:3568055/1‑100 (MQ=255)

                                                               tCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTc                                   <  2:5253854/100‑1 (MQ=255)

                                                                cccGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCa                                  >  2:581071/1‑100 (MQ=255)

                                                                 ccGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAc                                 >  2:2865717/1‑100 (MQ=255)

                                                                  cGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAcc                                <  1:1105704/100‑1 (MQ=255)

                                                                   gTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAccc                               <  2:5360420/100‑1 (MQ=255)

                                                                    ttGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGTGTAATCTTGTCAccc                               <  1:2040920/100‑1 (MQ=255)

                                                                    ttGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCg                              <  2:3642561/100‑1 (MQ=255)

                                                                    tagATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAATGGTGGTGGATGGCGAACCTGATTTACGAGTGCGTAATCTTGTCACCCg                              <  2:5477238/98‑1 (MQ=255)

                                                                       atatCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGttt                           <  2:5346306/100‑1 (MQ=255)

                                                                        tatCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAGACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTCGTCACCCGTTTc                          >  1:841839/1‑100 (MQ=255)

                                                                        tatCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTc                          >  1:1603539/1‑100 (MQ=255)

                                                                         atCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTcc                         >  1:3684930/1‑100 (MQ=255)

                                                                          tCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTccc                        >  1:1647410/1‑100 (MQ=255)

                                                                          ncGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTccc                        >  2:1182683/2‑100 (MQ=255)

                                                                          gcGCTTGAACATGCAGCGGAACAGG‑CCCGCCATGCGAGCAGAGCACGGTGGTGGATGGAGAACCTGTTTTACGAGTGCGTAAGCTTGTCACCGGTTTacc                        <  2:2501732/99‑1 (MQ=255)

                                                                           cGCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTCTCCCt                       >  1:1129138/1‑100 (MQ=255)

                                                                            gCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCtt                      >  1:4240596/1‑100 (MQ=255)

                                                                            gCTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCtt                      >  2:4190881/1‑100 (MQ=255)

                                                                             cTTGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCttt                     >  2:5499082/1‑100 (MQ=255)

                                                                              ttGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTg                    <  1:1495659/100‑1 (MQ=255)

                                                                               tGAACATCCAGCGAAACAGG‑CCCTCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGATTTACGAGTGCGTAATCTTGTAACCCGTTTCCCTTTgc                   >  2:4607700/1‑100 (MQ=255)

                                                                               tGAACATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgc                   >  2:1201524/1‑100 (MQ=255)

                                                                                 aaCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTgcgc                 >  1:500339/1‑100 (MQ=255)

                                                                                  aCATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCa                <  1:383608/100‑1 (MQ=255)

                                                                                   cATCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGGAATCTTGTCACCCGTTTCCCTTTGCGCAg               >  1:5089025/1‑100 (MQ=255)

                                                                                    aTCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAATGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCTCAGc              <  2:4646687/100‑1 (MQ=255)

                                                                                    aTCCAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGc              <  2:3473931/100‑1 (MQ=255)

                                                                                      ccAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCgg            <  2:2533469/100‑1 (MQ=255)

                                                                                       cAGCGAAACAGGCCCCCCCC‑TCGAGACTACAACGGCGGTGGATGGCAGCACTGTTTCACGAGACCGTAATCATGTCTGCCGTTTCCCTTTGCGCAGCGGt           >  2:2710843/1‑100 (MQ=255)

                                                                                       cAGCGAAACAGG‑CCTACCCATCGAGCAGAAAACGGTGGTCGCTGCAGAACTTGTTTTACGAGTGACTCATCTTTTCTACCGTTTCCCCTTTCGAAGCgtg           >  2:2203759/1‑98 (MQ=255)

                                                                                       cAGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGt           >  1:1409698/1‑100 (MQ=255)

                                                                                        aGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGtt          >  2:4747783/1‑100 (MQ=255)

                                                                                        aGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGtt          <  1:4064555/100‑1 (MQ=255)

                                                                                        aGCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGtt          >  1:4559226/1‑100 (MQ=255)

                                                                                         gCGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGttt         <  1:1568893/100‑1 (MQ=255)

                                                                                          cGAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttg        <  1:3096446/100‑1 (MQ=255)

                                                                                           gAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgt       <  2:2586629/100‑1 (MQ=255)

                                                                                           gAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgt       >  1:3350168/1‑100 (MQ=255)

                                                                                           gAAACAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgt       <  1:2513522/100‑1 (MQ=255)

                                                                                             aaCAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgttg     <  2:2592870/100‑1 (MQ=255)

                                                                                             aaCAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgttg     >  2:4028362/1‑100 (MQ=255)

                                                                                             aaCAGG‑CCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgttg     <  2:2738958/100‑1 (MQ=255)

                                                                                                 ggCCCCCCCCATCGAGCAGAAAACGGTCGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAAt  >  1:5386415/1‑100 (MQ=255)

GTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAAT  >  NC_000913/868316‑868512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A