Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,438,947 T→G E613A (GAA→GCA)  pfo ← pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,438,9470TG99.1% 776.9 / ‑2.2 216E613A (GAA→GCA) pfopyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (99/114);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 220 total aligned reads displayed.

CGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTTCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAAT  >  NC_000913/1438850‑1439042
                                                                                                 |                                                                                               
 gCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCAGCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTa                                                                                              >  2:5151815/1‑100 (MQ=255)
  cAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTAc                                                                                             <  2:40993/100‑1 (MQ=255)
   aGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACg                                                                                            <  1:5558166/100‑1 (MQ=255)
    gCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAGCCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACgg                                                                                           <  2:2721206/100‑1 (MQ=255)
    gCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACgg                                                                                           >  2:3273822/1‑100 (MQ=255)
        ttACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTc                                                                                       >  1:722074/1‑100 (MQ=255)
           cGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcg                                                                                    >  1:2933900/1‑100 (MQ=255)
           cGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcg                                                                                    >  1:2865885/1‑100 (MQ=255)
           cGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcg                                                                                    >  1:2579417/1‑100 (MQ=255)
            ggTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcgc                                                                                   <  1:3570353/100‑1 (MQ=255)
            ggTTTTCACGAAATCAGGGGCGGCATCGAAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcgc                                                                                   >  1:4897994/1‑100 (MQ=255)
             gTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcgcg                                                                                  <  1:1780028/100‑1 (MQ=255)
                ttCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTGACCGGTTGCAACGGAACTTCTTCTACGGATTCACgcgcca                                                                               >  1:4919294/1‑100 (MQ=255)
                  cACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagc                                                                             >  1:470276/1‑100 (MQ=255)
                   aCGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagcg                                                                            >  1:33920/1‑100 (MQ=255)
                   aCGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagcg                                                                            <  1:2327463/100‑1 (MQ=255)
                     gAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagcgcc                                                                          <  1:3666547/100‑1 (MQ=255)
                      aaaTCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagcgcca                                                                         >  1:194674/1‑100 (MQ=255)
                      aaaTCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagcgcca                                                                         >  2:3597989/1‑100 (MQ=255)
                       aaTCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGcgccagcgccag                                                                        <  1:3299155/100‑1 (MQ=255)
                        aTCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCaga                                                                       <  1:3333588/100‑1 (MQ=255)
                         tCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCagag                                                                      <  2:3072559/100‑1 (MQ=255)
                           aGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGcc                                                                    >  2:433030/1‑100 (MQ=255)
                           aGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGcc                                                                    >  1:101786/1‑100 (MQ=255)
                           aGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGcc                                                                    <  2:2873452/100‑1 (MQ=255)
                             gggCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTg                                                                  >  2:1179405/1‑100 (MQ=255)
                              ggcggcATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGc                                                                 >  2:2201097/1‑100 (MQ=255)
                               gcggcATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGcc                                                                >  2:449752/1‑100 (MQ=255)
                                 ggcATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAg                                                              <  2:52286/100‑1 (MQ=255)
                                  gcATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGt                                                             <  1:781928/100‑1 (MQ=255)
                                   cATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGtt                                                            >  2:2670816/1‑100 (MQ=255)
                                   cATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGtt                                                            >  1:2222043/1‑100 (MQ=255)
                                    aTCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTg                                                           >  2:149738/1‑100 (MQ=255)
                                    aTCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTg                                                           <  1:3965030/100‑1 (MQ=255)
                                    aTCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTg                                                           >  1:2453298/1‑100 (MQ=255)
                                    aTCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTg                                                           <  1:4153434/100‑1 (MQ=255)
                                    aTCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTg                                                           >  1:1498184/1‑100 (MQ=255)
                                     tCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGATCCTGCCAGTTgg                                                          <  2:1361745/100‑2 (MQ=255)
                                      cGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCg                                                         <  1:1713687/100‑1 (MQ=255)
                                        gAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACGGGTTGCAACGGAACTTCTACTACGGATTCACGCGCCAGCGCCACAGCCTGCCAGTTTCGtt                                                       >  2:1743055/1‑100 (MQ=255)
                                        gAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGtt                                                       <  1:859141/100‑1 (MQ=255)
                                          aaCCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTcc                                                     <  2:187546/100‑1 (MQ=255)
                                          aaCCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTcc                                                     <  2:2487080/100‑1 (MQ=255)
                                            ccACTGGCGGTCGATTGGCGCTCTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccac                                                   <  1:5066069/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGGGCCTGCCAGTTGCGTTccacc                                                  <  2:206590/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                  <  1:1072587/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                  <  1:3247820/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                  <  1:2696838/100‑1 (MQ=255)
                                                tGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGa                                               <  1:4400344/100‑1 (MQ=255)
                                                 ggCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGAt                                              >  1:118456/1‑100 (MQ=255)
                                                   cGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATcc                                            >  1:4100403/1‑100 (MQ=255)
                                                    ggTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCt                                           >  2:3968661/1‑100 (MQ=255)
                                                     gTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTg                                          >  2:3960056/1‑100 (MQ=255)
                                                     gTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTg                                          >  2:1635612/1‑100 (MQ=255)
                                                      tCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCTTTCCACCAGATCCTgg                                         <  2:5316531/100‑1 (MQ=255)
                                                      tCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTgg                                         <  1:4186592/100‑1 (MQ=255)
                                                      tCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTgg                                         <  1:580406/100‑1 (MQ=255)
                                                        gATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGcc                                       <  1:3845377/100‑1 (MQ=255)
                                                         aTTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCt                                      >  1:2509995/1‑100 (MQ=255)
                                                          tctgcgcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCtt                                     <  2:1516905/97‑1 (MQ=255)
                                                            ggCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTg                                   >  1:5378976/1‑100 (MQ=255)
                                                             gcgcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGc                                  <  2:3987729/100‑1 (MQ=255)
                                                             gcgcGGTGCGGGTTTACCGGTTGCAACGGAACGTCTGCTACGGATGCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGc                                  <  2:3192844/100‑1 (MQ=255)
                                                              cgcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCt                                 >  2:3516486/1‑100 (MQ=255)
                                                               gcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTa                                >  2:4693872/1‑100 (MQ=255)
                                                                cTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTAc                               <  2:1736040/100‑1 (MQ=255)
                                                                cTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTAc                               <  1:4794204/100‑1 (MQ=255)
                                                                 tgtgCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACt                              <  2:2053470/100‑1 (MQ=255)
                                                                 tgtgCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACt                              <  2:1730996/100‑1 (MQ=255)
                                                                  gtgCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTTCACCAGATCCTGGCCTTTGCTACTg                             <  2:4380395/100‑1 (MQ=255)
                                                                    gCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGtn                           <  1:1470718/100‑2 (MQ=255)
                                                                    gCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTa                           <  2:3777897/100‑1 (MQ=255)
                                                                      ggATTTACCGGTTGCAACGGAACCTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAAc                         <  1:1655206/100‑1 (MQ=255)
                                                                       gATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACt                        >  1:3873704/1‑100 (MQ=255)
                                                                       gATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACt                        >  1:2331747/1‑100 (MQ=255)
                                                                         tttACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACttt                      <  1:1570446/100‑1 (MQ=255)
                                                                         tttACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACttt                      >  1:1634595/1‑100 (MQ=255)
                                                                         tttACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACttt                      <  1:5178848/100‑1 (MQ=255)
                                                                         tttACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACttt                      >  2:3230430/1‑100 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                    <  2:707945/100‑1 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                    <  1:1160311/100‑1 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                    <  2:4867808/100‑1 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                    >  2:3456440/1‑100 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                    <  2:2906271/100‑1 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                    <  1:458457/100‑1 (MQ=255)
                                                                            aCCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTgg                   >  1:1899564/1‑100 (MQ=255)
                                                                            aCCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTgg                   >  1:2166089/1‑100 (MQ=255)
                                                                            aCCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTgg                   >  2:457420/1‑100 (MQ=255)
                                                                            aCCGCTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCATGGCCTTTGCTACTGTAACTTTTgg                   <  1:4997565/100‑1 (MQ=255)
                                                                             ccGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGc                  <  1:2118318/100‑1 (MQ=255)
                                                                             ccGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGc                  <  2:1237306/100‑1 (MQ=255)
                                                                                  tGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCg             >  1:176605/1‑100 (MQ=255)
                                                                                   gCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGc            >  1:1332336/1‑100 (MQ=255)
                                                                                   gCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGc            >  1:5313589/1‑100 (MQ=255)
                                                                                     aaCGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTGTGCTACTGTAACTTTTGGCAATCGCAc          >  2:1911137/1‑100 (MQ=255)
                                                                                      aCGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCAcc         >  2:3832296/1‑100 (MQ=255)
                                                                                       cGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCAccc        <  1:3051099/100‑1 (MQ=255)
                                                                                       cGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCAccc        <  2:5306399/100‑1 (MQ=255)
                                                                                         gAACTTCTGCTACGGATTCACGCGCCCGCGCCAGTGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTg      <  1:606837/100‑1 (MQ=255)
                                                                                         gAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTg      <  2:2427078/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTTCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAAT  >  NC_000913/1438850‑1439042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A