Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,438,947 T→G E613A (GAA→GCA)  pfo ← pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,438,9470TG99.3% 523.9 / ‑4.2 147E613A (GAA→GCA) pfopyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (59/87);  total (59/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 156 total aligned reads displayed.

CGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTTCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATT  >  NC_000913/1438850‑1439043
                                                                                                 |                                                                                                
 gCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTa                                                                                               >  2:2809852/1‑100 (MQ=255)
 gCAGCGGTTACGGTTTTCACGAAACCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTa                                                                                               <  2:3355228/100‑1 (MQ=255)
    gCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACgg                                                                                            <  1:1551161/100‑1 (MQ=255)
      ggTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTCCCGGTTGCAACCGAACTTCTGCTACGGAt                                                                                          >  1:1676628/1‑100 (MQ=255)
      ggTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGAt                                                                                          >  2:3202757/1‑100 (MQ=255)
      ggTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACCGAACTTCTGCTACGGAt                                                                                          >  1:1674510/1‑100 (MQ=255)
       gTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGAtt                                                                                         >  2:3081091/1‑100 (MQ=255)
       gTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGAtt                                                                                         >  2:2975659/1‑100 (MQ=255)
         tACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCa                                                                                       <  1:3020578/100‑1 (MQ=255)
         tACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCa                                                                                       >  1:911023/1‑100 (MQ=255)
          aCGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAc                                                                                      <  1:3707132/100‑1 (MQ=255)
          aCGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAc                                                                                      <  2:1339506/100‑1 (MQ=255)
          aCGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAc                                                                                      >  2:1500605/1‑100 (MQ=255)
           cGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcg                                                                                     <  1:612952/100‑1 (MQ=255)
             gTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcgcg                                                                                   <  1:573472/100‑1 (MQ=255)
             gTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAcgcg                                                                                   >  1:2306110/1‑100 (MQ=255)
              ttttCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgc                                                                                  >  2:414047/1‑100 (MQ=255)
               tttCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgcc                                                                                 <  2:2043441/100‑1 (MQ=255)
               tttCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgcc                                                                                 <  2:2024963/100‑1 (MQ=255)
                  cACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagc                                                                              >  2:2799495/1‑100 (MQ=255)
                  cACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACgcgccagc                                                                              >  1:1359145/1‑100 (MQ=255)
                   aCGAAATGAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGGTACGGATTCACgcgccagcg                                                                             <  2:1172037/100‑1 (MQ=255)
                       aaTCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGcgccagcgccag                                                                         >  1:1795813/1‑100 (MQ=255)
                       aaTCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGcgccagcgccag                                                                         >  1:1279043/1‑100 (MQ=255)
                         tCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCagag                                                                       <  2:1350591/100‑1 (MQ=255)
                           aGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGcc                                                                     <  1:1307852/100‑1 (MQ=255)
                           aGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGcc                                                                     >  1:319447/1‑100 (MQ=255)
                            ggggCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCt                                                                    >  1:537028/1‑100 (MQ=255)
                             gggCGGCATCGGAAACCACTCGCGGTCGATTGGCGCTGTTCGGGTTTACCGGTTGCAACGGAACTTCTGCTACGGATTCCCGCGCCAGCGCCAGAGCCTg                                                                   >  2:1618702/1‑100 (MQ=255)
                                  gcATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGt                                                              >  1:3066659/1‑100 (MQ=255)
                                   cATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGtt                                                             >  2:3743928/1‑100 (MQ=255)
                                   cATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGtt                                                             <  1:3150752/100‑1 (MQ=255)
                                   cATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGtt                                                             >  2:610547/1‑100 (MQ=255)
                                    aTCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTg                                                            >  1:1808059/1‑100 (MQ=255)
                                     tCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGc                                                           <  1:2851247/100‑1 (MQ=255)
                                       ggAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGt                                                         >  1:2591061/1‑100 (MQ=255)
                                         aaaCCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTc                                                       <  2:2327629/100‑1 (MQ=255)
                                          aaCCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTcc                                                      <  1:893364/100‑1 (MQ=255)
                                            ccACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccac                                                    <  1:211122/100‑1 (MQ=255)
                                            ccACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccac                                                    >  2:354748/1‑100 (MQ=255)
                                            ccACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccac                                                    >  2:302953/1‑100 (MQ=255)
                                             gaCTGGCGGTCGATTGGCGCTGTGCGGATTTACCTGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTacacc                                                   <  2:3400146/99‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                   <  1:1231568/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                   <  2:2657387/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                   <  2:2487160/100‑1 (MQ=255)
                                             cACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacc                                                   <  2:2310994/100‑1 (MQ=255)
                                              aCTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacca                                                  >  1:1049335/1‑100 (MQ=255)
                                              aCTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacca                                                  <  1:2422586/100‑1 (MQ=255)
                                              aCTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTccacca                                                  >  1:1220151/1‑100 (MQ=255)
                                               cTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAg                                                 >  1:224432/1‑100 (MQ=255)
                                               cTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAg                                                 >  1:645781/1‑100 (MQ=255)
                                               cTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAg                                                 <  2:1212828/100‑1 (MQ=255)
                                                 ggCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGAt                                               <  1:3276548/100‑1 (MQ=255)
                                                  gCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATc                                              >  2:1776067/1‑100 (MQ=255)
                                                  gCGGTCGATGGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCAGGCGCCAGCGCCAGGGCCTGCCAGTTGCGTTCCACCAGATc                                              >  1:2869567/1‑100 (MQ=255)
                                                   cGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTTCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATcc                                             <  1:2864300/100‑1 (MQ=255)
                                                   cGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATcc                                             <  2:1027629/100‑1 (MQ=255)
                                                     gTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTg                                           <  2:576236/100‑1 (MQ=255)
                                                     gTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTg                                           <  1:1363102/100‑1 (MQ=255)
                                                       cGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGCACTTCTGCTACGGCTTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGc                                         <  2:333468/100‑1 (MQ=255)
                                                       cGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTggn                                         <  1:3256870/100‑2 (MQ=255)
                                                        gATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGcc                                        <  2:1073882/100‑1 (MQ=255)
                                                        gATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGcc                                        <  2:463958/100‑1 (MQ=255)
                                                        gATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGcc                                        <  1:1635672/100‑1 (MQ=255)
                                                           tGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCttc                                     <  1:413021/100‑2 (MQ=255)
                                                             gcgcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTTGCCTTTGc                                   <  2:3835119/100‑1 (MQ=255)
                                                             gcgcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGc                                   <  2:357028/100‑1 (MQ=255)
                                                              cgcTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCt                                  <  1:1954197/100‑1 (MQ=255)
                                                                cTGTGCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTTCTAc                                <  2:3382961/100‑1 (MQ=255)
                                                                  gtgCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTg                              >  1:3683254/1‑100 (MQ=255)
                                                                  gtgCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTg                              <  1:3761710/100‑1 (MQ=255)
                                                                   tgCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGt                             <  1:1522042/100‑1 (MQ=255)
                                                                    gCGGATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTa                            >  1:1888233/1‑100 (MQ=255)
                                                                      ggATTTACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAAc                          <  2:1817177/100‑1 (MQ=255)
                                                                         tttACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACttt                       >  2:206878/1‑100 (MQ=255)
                                                                         tttACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACttt                       >  2:3862587/1‑100 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                     <  1:3000770/100‑1 (MQ=255)
                                                                           tACCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTg                     <  2:2270859/100‑1 (MQ=255)
                                                                            aCCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTgg                    <  1:1462872/100‑1 (MQ=255)
                                                                            aCCGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCAAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTgg                    >  1:3573131/1‑100 (MQ=255)
                                                                             ccGGTTGCTACCGAACTTCTGCTACGGATTCACGCGCCTGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGc                   <  1:3906982/100‑1 (MQ=255)
                                                                             ccGGTTGCAACGGAAGTTCTGCTACGGATTGACGCGCCAGAGCCACAGCCTGCCAGTTGCGTTCCACCAGATACTGGCCTTTGCTACTGTAACTTTTGGc                   >  1:3541517/1‑100 (MQ=255)
                                                                             ccGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGc                   <  1:2065852/100‑1 (MQ=255)
                                                                             ccGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGc                   <  2:2328359/100‑1 (MQ=255)
                                                                              cGGTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCa                  >  1:1478428/1‑100 (MQ=255)
                                                                               ggTTGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCaa                 <  1:4050735/100‑1 (MQ=255)
                                                                                 ttGCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATc               <  1:174799/100‑1 (MQ=255)
                                                                                   gCAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGc             >  2:1160654/1‑100 (MQ=255)
                                                                                    cAACGGAACTTCTGCTACGGATTCACGCGTCAGCGCCAGAGCCTGCCAGTTGCGTTCCGCCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCa            >  1:3909047/1‑100 (MQ=255)
                                                                                    cAACGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCa            >  1:546448/1‑100 (MQ=255)
                                                                                     aaCGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCAc           >  2:3282890/1‑100 (MQ=255)
                                                                                       cGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCAccc         >  2:2378265/1‑100 (MQ=255)
                                                                                       cGGAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCAccc         <  2:532399/100‑1 (MQ=255)
                                                                                        ggAACTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCt        >  2:2747770/1‑100 (MQ=255)
                                                                                          aaCTTCTGCTATTGTTTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGTTACTGTAACTTTTGGCAATCGCACCCTGc      <  2:2182941/100‑1 (MQ=255)
                                                                                          aaCTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGc      <  1:1414085/100‑1 (MQ=255)
                                                                                           aCTTCTGCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCa     <  2:2146330/100‑1 (MQ=255)
                                                                                            cttctgCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCaa    >  1:1050747/1‑100 (MQ=255)
                                                                                             ttctgcTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAAt   <  1:1628462/100‑1 (MQ=255)
                                                                                             ttctgcTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAAt   <  2:2406893/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CGCAGCGGTTACGGTTTTCACGAAATCAGGGGCGGCATCGGAAACCACTGGCGGTCGATTGGCGCTGTGCGGATTTACCGGTTGCAACGGAACTTCTTCTACGGATTCACGCGCCAGCGCCAGAGCCTGCCAGTTGCGTTCCACCAGATCCTGGCCTTTGCTACTGTAACTTTTGGCAATCGCACCCTGCAATT  >  NC_000913/1438850‑1439043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A