Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,921,059	T→G	P279P (CCT→CCG)	rsmF →	16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,921,059	0	T	G	99.0%	711.9 / NA	199	P279P (CCT→CCG)	rsmF	16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (104/92); minor base C (1/1); total (105/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

GTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACT  >  NC_000913/1920962‑1921155

gTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTTGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCggg                                                                                                >  2:3630836/1‑100 (MQ=255)

gTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCggg                                                                                                <  2:1288156/100‑1 (MQ=255)

  aCCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTg                                                                                              <  2:1153151/100‑1 (MQ=255)

  aCCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCCGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTg                                                                                              >  2:2412697/1‑100 (MQ=255)

   ccTTAAACCAGGCAGCAACCGAAGCCGTTTGCCTGTGGCCGAAAGAGCCTTCCCCCGACGCAGCAGAGTTTTTCCCACTTGGCGTTCCCTTCCCGGGTGc                                                                                             >  2:4398925/1‑100 (MQ=255)

   ccTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGc                                                                                             >  2:166310/1‑100 (MQ=255)

   ccTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGc                                                                                             <  2:237440/100‑1 (MQ=255)

   ccTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGc                                                                                             >  2:3079081/1‑100 (MQ=255)

    cTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGca                                                                                            >  2:4715700/1‑100 (MQ=255)

       aaaCCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGcaaac                                                                                         >  2:1852372/1‑100 (MQ=255)

       aaaCCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGcaaac                                                                                         <  1:103453/100‑1 (MQ=255)

          ccAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGcaaacaaa                                                                                      <  1:5354984/100‑1 (MQ=255)

          ccAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGcaaacaaa                                                                                      >  2:2725494/1‑100 (MQ=255)

            aGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAgc                                                                                    <  1:5157733/100‑1 (MQ=255)

              gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAgcgc                                                                                  <  1:5488157/100‑1 (MQ=255)

              gaagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAgcgc                                                                                  >  2:4768723/1‑100 (MQ=255)

               aagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCt                                                                                 >  1:4010413/1‑100 (MQ=255)

               aagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCt                                                                                 >  1:2746629/1‑100 (MQ=255)

               aagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCt                                                                                 <  1:2055057/100‑1 (MQ=255)

               aagaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCt                                                                                 >  2:3263777/1‑100 (MQ=255)

                agaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTg                                                                                >  2:1523333/1‑100 (MQ=255)

                agaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTg                                                                                >  1:4570328/1‑100 (MQ=255)

                agaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCAATCTCTTCCCGGGTGCAAACAAAGCGCTg                                                                                <  1:518863/100‑1 (MQ=255)

                 gaaAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTAGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGa                                                                               <  2:5087724/100‑1 (MQ=255)

                  aaaaCGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTCCCCCGACGCAGTCGCGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGAc                                                                              <  2:3022138/100‑1 (MQ=255)

                  aaaaCGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGAc                                                                              <  2:5184501/100‑1 (MQ=255)

                   aaaCGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGAcc                                                                             <  1:4977626/100‑1 (MQ=255)

                    aaCGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCg                                                                            >  2:2112459/1‑100 (MQ=255)

                      cGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGTGCTGACCgaa                                                                          <  1:4869491/100‑1 (MQ=255)

                      cGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGATTTTACCACTTGGCGATCTCTTCCCGGGTGCACACAAAGCGCTGACCgaa                                                                          >  1:2281476/1‑100 (MQ=255)

                       gAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGAccaata                                                                         >  2:2223729/1‑96 (MQ=255)

                       gAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCgaag                                                                         <  1:4483109/100‑1 (MQ=255)

                         aGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCgaagaa                                                                       <  2:4518809/100‑1 (MQ=255)

                           ccGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAgg                                                                     >  1:2875866/1‑100 (MQ=255)

                            cGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGc                                                                    >  1:4113015/1‑100 (MQ=255)

                               gtGCCTGCGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTGCCCCTTGGCGATCACTTCCCGGGTGCAAACAAAGCGCTCACCGAAGAAGGCttt                                                                 <  2:752771/99‑1 (MQ=255)

                                tGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCtttt                                                                >  2:2877186/1‑100 (MQ=255)

                                tGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCtttt                                                                >  2:3979123/1‑100 (MQ=255)

                                 gCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCttttt                                                               <  1:1365085/100‑1 (MQ=255)

                                 gCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCttttt                                                               <  1:5249222/100‑1 (MQ=255)

                                 gCCTGGGGCTGAAAGAGACTGACCCCGACGCAGTAGAGGTTTTACCACTGGGCTATCTCTTCGCGGGTGCAAACAAATCGCTGACCGGAGTATGCtttgt                                                               >  2:2493369/1‑98 (MQ=255)

                                  ccTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTg                                                              <  1:4728753/100‑1 (MQ=255)

                                    tgtgGCTGAAAGAGACTTACCCTGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCTAACAAAGCGCTGACCGAAGGAGGCTTTTTGCa                                                            >  2:2174636/1‑100 (MQ=255)

                                    tgtgGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCa                                                            >  1:4634947/1‑100 (MQ=255)

                                      tgGCTGAAAGAGACTTACCGCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTGCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATg                                                          <  2:1832717/100‑1 (MQ=255)

                                      tgGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATg                                                          >  1:5058477/1‑100 (MQ=255)

                                       ggCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGACGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGACGAAGGCTTTTTGCATGt                                                         >  1:4717598/1‑100 (MQ=255)

                                         cTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGttt                                                       >  2:3749996/1‑100 (MQ=255)

                                          tGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGtttt                                                      <  2:4564108/100‑1 (MQ=255)

                                           gAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTc                                                     <  1:3959820/100‑1 (MQ=255)

                                           gAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCACAGACCGAAGCCGGCTTCTTGCATGTTTTc                                                     >  2:2476439/1‑100 (MQ=255)

                                            aaaGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTcc                                                    <  1:3784745/100‑1 (MQ=255)

                                            aaaGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTcc                                                    >  2:4962241/1‑100 (MQ=255)

                                            aaaGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTcc                                                    >  1:1747699/1‑100 (MQ=255)

                                            aaaGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTcc                                                    >  1:1184406/1‑100 (MQ=255)

                                             aaGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTccc                                                   <  1:2481620/100‑1 (MQ=255)

                                               gagaCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCcac                                                 <  2:1065878/100‑1 (MQ=255)

                                                 gaCTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACaa                                               >  1:4238406/1‑100 (MQ=255)

                                                   cTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAAt                                             <  2:4089700/100‑1 (MQ=255)

                                                    ttACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAAtt                                            <  2:4663721/100‑1 (MQ=255)

                                                     tACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAAttt                                           >  1:1322723/1‑100 (MQ=255)

                                                     tACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAAttt                                           >  1:1454608/1‑100 (MQ=255)

                                                         ccGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGTGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGa                                       >  1:480718/1‑100 (MQ=255)

                                                         ccGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGa                                       >  2:301085/1‑100 (MQ=255)

                                                          cGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGAc                                      <  1:190440/100‑1 (MQ=255)

                                                           gACGCAGTAGAGTTTTTACCACTTGGCGATCTATTACAGGGTGCAAACAAAGCGATGACAGAAGAAGGCTTTTTGGATGTTTTCGCACAAATTTAGGACt                                     >  2:3818375/1‑100 (MQ=255)

                                                            aCGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTg                                    <  1:2810904/100‑1 (MQ=255)

                                                               cAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGa                                 <  2:1541446/100‑1 (MQ=255)

                                                                aGTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGaa                                >  2:3472477/1‑100 (MQ=255)

                                                                 gTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAg                               <  2:3661379/100‑1 (MQ=255)

                                                                 gTAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAg                               >  2:636378/1‑100 (MQ=255)

                                                                  tAGAGTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAgg                              >  1:2297927/1‑100 (MQ=255)

                                                                    gagTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGct                            <  2:4476998/100‑1 (MQ=255)

                                                                    gagTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGct                            <  2:1435730/100‑1 (MQ=255)

                                                                      gTTTTTACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttc                          >  2:2915543/1‑100 (MQ=255)

                                                                        ttttACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttctt                        >  1:1136892/1‑100 (MQ=255)

                                                                        ttttACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttctt                        >  2:1927066/1‑100 (MQ=255)

                                                                        ttttACCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGcttctt                        >  1:3824644/1‑100 (MQ=255)

                                                                            aCCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGtt                    <  2:386164/100‑1 (MQ=255)

                                                                            aCCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGtt                    <  2:5379068/100‑1 (MQ=255)

                                                                            aCCACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGtt                    <  1:354341/100‑1 (MQ=255)

                                                                             ccACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTg                   <  1:929835/100‑1 (MQ=255)

                                                                             ccACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTg                   <  1:3346951/100‑1 (MQ=255)

                                                                             ccACTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTg                   >  2:4271598/1‑100 (MQ=255)

                                                                               aCTTGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCt                 >  1:3079750/1‑100 (MQ=255)

                                                                                 ttGGCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCg               <  1:2436988/100‑1 (MQ=255)

                                                                                 ttGCCGAGCTCTGCACCGGTGCAAAAAAAACGCTGCCCGAAGACGGCTTTTAGACTGATTTCCCAAAAATTTACGACTGCGACGGCTTCTTCGTTGCtag               <  2:4932186/100‑3 (MQ=255)

                                                                                    gCGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCt            <  1:1873224/100‑1 (MQ=255)

                                                                                     cGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTTTTCGTTGCTCGTCTg           <  2:4181183/100‑1 (MQ=255)

                                                                                     cGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTg           <  1:3862582/100‑1 (MQ=255)

                                                                                     cGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTg           >  1:5441084/1‑100 (MQ=255)

                                                                                     cGATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAACGCTTCTTCGTTGCTCGTCTg           >  1:3182209/1‑100 (MQ=255)

                                                                                      gATCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGc          <  1:3026585/100‑1 (MQ=255)

                                                                                       aTCTCTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCg         >  2:3703280/1‑100 (MQ=255)

                                                                                        tctcTTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGt        <  1:502777/100‑1 (MQ=255)

                                                                                         ctctTCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTa       >  2:3569331/1‑100 (MQ=255)

                                                                                             tCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAAc   <  1:3658856/100‑1 (MQ=255)

                                                                                             tCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAAc   >  1:3801325/1‑100 (MQ=255)

                                                                                             tCCCGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAAc   <  1:4214868/100‑1 (MQ=255)

                                                                                              cccGGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACt  >  1:2205088/1‑100 (MQ=255)

GTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTTTCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACT  >  NC_000913/1920962‑1921155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A