Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,075,512 T→G L245R (CTG→CGG)  yeeR → CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,075,5120TG98.6% 766.6 / ‑2.4 216L245R (CTG→CGG) yeeRCP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (100/113);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

TGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAACCTGT  >  NC_000913/2075415‑2075610
                                                                                                 |                                                                                                  
tGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGa                                                                                                  <  1:5385050/100‑1 (MQ=255)
 ggCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAg                                                                                                 <  1:4792531/100‑1 (MQ=255)
 ggCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAg                                                                                                 <  2:2534138/100‑1 (MQ=255)
  gCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAgg                                                                                                <  2:2959686/100‑1 (MQ=255)
   cTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAggg                                                                                               >  2:111224/1‑100 (MQ=255)
   cTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAggg                                                                                               >  1:2378556/1‑100 (MQ=255)
    tGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGa                                                                                              <  1:4132774/100‑1 (MQ=255)
    tGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGa                                                                                              >  1:2037249/1‑100 (MQ=255)
      aCCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAAt                                                                                            <  2:428251/100‑1 (MQ=255)
        cAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAAttt                                                                                          >  1:4388669/1‑100 (MQ=255)
         aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                         <  2:747832/100‑1 (MQ=255)
         aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                         <  2:627683/100‑1 (MQ=255)
         aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                         <  2:1381645/100‑1 (MQ=255)
         aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                         <  1:4868790/100‑1 (MQ=255)
          gTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGa                                                                                        >  1:3167730/1‑100 (MQ=255)
             tGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACt                                                                                     <  1:996068/100‑1 (MQ=255)
               cAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTgg                                                                                   >  2:287071/1‑100 (MQ=255)
                aaaaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGc                                                                                  >  1:3422382/1‑100 (MQ=255)
                  aaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAg                                                                                <  1:1254288/100‑1 (MQ=255)
                       ttACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGGGCGGAGGGAATTTGAACTGGCAGACGGa                                                                           <  2:2798694/100‑1 (MQ=255)
                         aCAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGaaa                                                                         >  2:2162198/1‑100 (MQ=255)
                          cAGCCGTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATGTGAACTGGCAGACGGAAAc                                                                        >  2:2494298/1‑100 (MQ=255)
                           aGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACt                                                                       <  1:4181330/100‑1 (MQ=255)
                           aGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACt                                                                       <  2:5218723/100‑1 (MQ=255)
                            gCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTg                                                                      >  1:1794523/1‑100 (MQ=255)
                            gCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTg                                                                      <  2:4333979/100‑1 (MQ=255)
                            gCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTg                                                                      <  1:927342/100‑1 (MQ=255)
                              cTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGt                                                                    >  1:704303/1‑100 (MQ=255)
                              cTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGt                                                                    <  2:497426/100‑1 (MQ=255)
                                tCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTa                                                                  >  1:2176045/1‑100 (MQ=255)
                                 ccTGCCTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGAGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTAc                                                                 <  1:2519188/100‑1 (MQ=255)
                                   tGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTg                                                               <  1:647963/100‑1 (MQ=255)
                                    gACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGa                                                              >  1:2924613/1‑100 (MQ=255)
                                    gACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGa                                                              <  2:3724821/100‑1 (MQ=255)
                                    gACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGa                                                              >  2:5399874/1‑100 (MQ=255)
                                     aCTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGaa                                                             <  2:3139903/100‑1 (MQ=255)
                                     aCTCACTGACGGAGCGCATCTGGCTGACGATCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGaa                                                             <  1:3818413/100‑1 (MQ=255)
                                       tCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACa                                                           <  1:4971870/100‑1 (MQ=255)
                                        cACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACaa                                                          >  2:1215945/1‑100 (MQ=255)
                                         aCTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAAt                                                         >  1:4009642/1‑100 (MQ=255)
                                         aCTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAAt                                                         >  1:2253269/1‑100 (MQ=255)
                                            gACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGcc                                                      <  2:3355945/100‑1 (MQ=255)
                                              cGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTg                                                    >  1:2570345/1‑100 (MQ=255)
                                               ggAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTAGTTACTGAACAATGCCTgg                                                   <  2:5028736/100‑1 (MQ=255)
                                                gAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGt                                                  >  1:2635050/1‑100 (MQ=255)
                                                 aGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTa                                                 >  2:3226730/1‑100 (MQ=255)
                                                 aGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTa                                                 <  2:2036579/100‑1 (MQ=255)
                                                 aGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTa                                                 <  1:1128695/100‑1 (MQ=255)
                                                 aGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTa                                                 <  2:2354629/100‑1 (MQ=255)
                                                  gcgcATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTAt                                                <  2:1783717/100‑1 (MQ=255)
                                                     cATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaa                                             >  2:337354/1‑100 (MQ=255)
                                                     cATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaa                                             >  2:242733/1‑100 (MQ=255)
                                                      aTCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaa                                            >  2:1349557/1‑100 (MQ=255)
                                                      aTCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaa                                            <  2:4222047/100‑1 (MQ=255)
                                                         tGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaagaa                                         >  1:513827/1‑100 (MQ=255)
                                                         tGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaagaa                                         >  1:1352687/1‑100 (MQ=255)
                                                          ggCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaagaaa                                        <  1:3731884/100‑1 (MQ=255)
                                                          ggCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaagaaa                                        >  2:1530053/1‑100 (MQ=255)
                                                           gCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAAc                                       >  2:3863246/1‑100 (MQ=255)
                                                           gCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAAc                                       >  1:1478101/1‑100 (MQ=255)
                                                           gCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAAc                                       <  2:1750044/100‑1 (MQ=255)
                                                             tGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACAt                                     >  1:5393661/1‑100 (MQ=255)
                                                                cGCTCATTTCTCACCGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGc                                  <  1:3212220/100‑1 (MQ=255)
                                                                 ggtgaTTTCTCAACGGATTTCCCAGGGAGAGCGGCGGGAATTTGAACTGGCGGACGGCTACTGGTTACTGAATAATGCCTGGTATGAAAGCAACATGACa                                 >  2:1607487/5‑100 (MQ=255)
                                                                 gCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCa                                 >  2:4952177/1‑100 (MQ=255)
                                                                 gCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCa                                 <  1:1067014/100‑1 (MQ=255)
                                                                  cTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAg                                >  1:1149175/1‑100 (MQ=255)
                                                                  cTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAg                                <  1:5473116/100‑1 (MQ=255)
                                                                   tCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAgg                               <  1:3949886/100‑1 (MQ=255)
                                                                    cATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAggg                              >  1:10735/1‑100 (MQ=255)
                                                                     aTTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGt                             >  1:773897/1‑100 (MQ=255)
                                                                        tctcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTa                          >  1:3083459/1‑100 (MQ=255)
                                                                        tctcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTa                          <  2:1305649/100‑1 (MQ=255)
                                                                        tctcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTa                          >  1:164122/1‑100 (MQ=255)
                                                                          tcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAAc                        <  2:1841342/100‑1 (MQ=255)
                                                                          tcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAAc                        <  2:4589150/100‑1 (MQ=255)
                                                                          tcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAAc                        >  2:3706570/1‑100 (MQ=255)
                                                                           cAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACg                       >  1:3513559/1‑100 (MQ=255)
                                                                           cAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACg                       >  2:3965035/1‑100 (MQ=255)
                                                                            aaCGGATTTCCCGGGGTGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTATTGAACAGTGACTGGTGCGTAAGAAATATGGCGGGGGTTAACgg                      >  2:829107/1‑99 (MQ=255)
                                                                              cGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAAc                    <  1:2778594/100‑1 (MQ=255)
                                                                                gATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAg                  <  1:4722342/100‑1 (MQ=255)
                                                                                 aTTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGt                 <  1:4491042/100‑1 (MQ=255)
                                                                                   ttCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTg               <  1:4295855/100‑1 (MQ=255)
                                                                                   ttCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTg               >  2:169125/1‑100 (MQ=255)
                                                                                   ttCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTg               <  2:4464026/100‑1 (MQ=255)
                                                                                     cccGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGaa             <  2:3603940/100‑1 (MQ=255)
                                                                                     cccGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGaa             >  2:4752969/1‑100 (MQ=255)
                                                                                      ccGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGaaa            >  1:1279799/1‑100 (MQ=255)
                                                                                      ccGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGaaa            <  2:294058/100‑1 (MQ=255)
                                                                                      ccGGGGAGAGCGGAGGGAATTTGAACTGACAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACCGTTGaaa            >  1:3464830/1‑100 (MQ=255)
                                                                                       cGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAag           <  1:3656672/100‑1 (MQ=255)
                                                                                       cGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAag           >  1:3257569/1‑100 (MQ=255)
                                                                                       cGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAag           <  1:4039534/100‑1 (MQ=255)
                                                                                       cGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAag           <  2:2206758/100‑1 (MQ=255)
                                                                                        ggggAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAaga          <  1:3216988/100‑1 (MQ=255)
                                                                                         gggAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAagag         >  2:410701/1‑100 (MQ=255)
                                                                                             gagCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAAcc     <  2:2307589/100‑1 (MQ=255)
                                                                                              agCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAATAGTTGAAAGAGAACCt    <  2:5317553/100‑1 (MQ=255)
                                                                                                cTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAACCTGt  >  1:1892973/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
TGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAACCTGT  >  NC_000913/2075415‑2075610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A