Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,075,512 T→G L245R (CTG→CGG)  yeeR → CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,075,5120TG98.1% 539.2 / 0.0 157L245R (CTG→CGG) yeeRCP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (71/83);  total (72/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 157 total aligned reads displayed.

GGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAACC  >  NC_000913/2075416‑2075607
                                                                                                |                                                                                               
ggCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAg                                                                                              <  2:743607/100‑1 (MQ=255)
   tGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGa                                                                                           >  2:592953/1‑100 (MQ=255)
   tGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGa                                                                                           >  1:3225224/1‑100 (MQ=255)
       cAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAAttt                                                                                       <  1:678564/100‑1 (MQ=255)
       cAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAAttt                                                                                       >  2:651718/1‑100 (MQ=255)
        aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                      <  2:1228115/100‑1 (MQ=255)
        aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                      >  1:3318371/1‑100 (MQ=255)
        aGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTg                                                                                      >  2:437473/1‑100 (MQ=255)
         gTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGa                                                                                     <  1:1186101/100‑1 (MQ=255)
         gTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGa                                                                                     >  1:3126467/1‑100 (MQ=255)
         gTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGa                                                                                     >  2:3883545/1‑100 (MQ=255)
          tttGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGaa                                                                                    <  2:2804688/100‑1 (MQ=255)
             gCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTg                                                                                 <  2:870974/100‑1 (MQ=255)
             gCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTg                                                                                 <  2:3844381/100‑1 (MQ=255)
              cAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTgg                                                                                >  2:2950910/1‑100 (MQ=255)
              cAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTgg                                                                                <  1:2435847/100‑1 (MQ=255)
              cAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATCTCTCAACGGATTCCCAGGGGAGAGCGGAGGGAATTTGAACTgg                                                                                >  2:2392906/1‑100 (MQ=255)
               aaaaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGc                                                                               <  1:2783521/100‑1 (MQ=255)
               aaaaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGc                                                                               <  2:3154780/100‑1 (MQ=255)
               aaaaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGc                                                                               <  1:2720547/100‑1 (MQ=255)
               aaaaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGc                                                                               >  1:3569042/1‑100 (MQ=255)
                 aaCGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAg                                                                             <  1:1818682/100‑1 (MQ=255)
                    gATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACg                                                                          <  1:3013303/100‑1 (MQ=255)
                     aTTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACgg                                                                         <  2:3878478/100‑1 (MQ=255)
                     aTTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACgg                                                                         <  2:3405798/100‑1 (MQ=255)
                      ttACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGa                                                                        <  1:2367361/100‑1 (MQ=255)
                      ttACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGa                                                                        <  2:2026303/100‑1 (MQ=255)
                       tACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGaa                                                                       >  2:1961846/1‑100 (MQ=255)
                         cAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAAc                                                                     >  1:3488766/1‑100 (MQ=255)
                         cAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAAc                                                                     <  1:2911192/100‑1 (MQ=255)
                           gCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTg                                                                   >  1:2074937/1‑100 (MQ=255)
                            ccTTCCTGACTCACTGACGGAGCGCATCTGTCTGACGCTCATTTCTCAACCGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGGACCTgg                                                                  <  2:3762133/100‑1 (MQ=255)
                            ccTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTgg                                                                  <  1:3602529/100‑1 (MQ=255)
                             cTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGt                                                                 <  2:2320301/100‑1 (MQ=255)
                              ttCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGtt                                                                >  1:3996716/1‑100 (MQ=255)
                                ccTGACTCACTGACGGNGCGNATNTGNCTGACGCTCATTTCTCAGCGCATTTCCCGGGTAGAGCGGAGGGAATTTGAACTGGCCGATGGAAACTGGTTAc                                                              <  2:772807/100‑1 (MQ=255)
                                ccTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTAc                                                              <  2:2250433/100‑1 (MQ=255)
                                 cTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACt                                                             <  1:368833/100‑1 (MQ=255)
                                  tGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTg                                                            <  2:3370731/100‑1 (MQ=255)
                                  tGACTCACTGACGGAGCGCATCGGGCTGACGGTCATTTCTAAACGGATTTCCCGGGGAGGGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTg                                                            <  2:1661752/100‑1 (MQ=255)
                                   gACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGa                                                           >  2:2455251/1‑100 (MQ=255)
                                   gACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGa                                                           >  1:1138466/1‑100 (MQ=255)
                                     cTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAAc                                                         <  1:2644151/100‑1 (MQ=255)
                                     cTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAAc                                                         >  1:331462/1‑100 (MQ=255)
                                     cTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATATCCCGGGGAGAGCGGAGGGAATCTGAACTGGCAGACGGAAACTGGTTACTGAAc                                                         <  2:3807996/100‑1 (MQ=255)
                                      tCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACa                                                        <  1:770986/100‑1 (MQ=255)
                                       cACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACaa                                                       >  1:1946391/1‑100 (MQ=255)
                                       cACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACaa                                                       >  2:1355929/1‑100 (MQ=255)
                                       cACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACaa                                                       >  2:3241075/1‑100 (MQ=255)
                                       cACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACaa                                                       >  1:2335957/1‑100 (MQ=255)
                                         cTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATg                                                     >  1:708520/1‑100 (MQ=255)
                                         cTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATg                                                     >  2:2886323/1‑100 (MQ=255)
                                           gACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGcc                                                   >  2:2538101/1‑100 (MQ=255)
                                           gACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGcc                                                   >  1:3478248/1‑100 (MQ=255)
                                            aCGGCGCGCATCTGGCTGACGCTCATTTCTAAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGCCAGACGGAAACTGGTTACTGAACAATGCCt                                                  <  2:180805/100‑1 (MQ=255)
                                             cGGATCGCATCTTGCTGACGCTCATTTCTCCGCGGATTACCCGGTTAGAGGGGAGTGAATTTTAACTTACAGACGGAAACTGGTTACTGAACAATGCCTg                                                 <  2:3595386/100‑1 (MQ=255)
                                             cGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTg                                                 >  1:2212576/1‑100 (MQ=255)
                                              ggAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTgg                                                >  1:1325897/1‑100 (MQ=255)
                                                aGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGCAACTGGTTACTGAACCATGCCTGGTa                                              >  1:3111871/1‑100 (MQ=255)
                                                    cATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaa                                          <  2:2371353/100‑1 (MQ=255)
                                                    cATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaa                                          <  2:3615115/100‑1 (MQ=255)
                                                    cATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACGGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaa                                          <  2:2095420/100‑1 (MQ=255)
                                                     aTCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTAtagat                                         >  1:637561/1‑96 (MQ=255)
                                                     aTCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaa                                         <  1:3109506/100‑1 (MQ=255)
                                                     aTCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaa                                         <  1:1707140/100‑1 (MQ=255)
                                                      tCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaag                                        <  1:2668879/100‑1 (MQ=255)
                                                         ggCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaagaaa                                     <  2:2645086/100‑1 (MQ=255)
                                                         ggCTGACGCTCATTTCTCAACGGAGTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATgaaagaaa                                     <  2:3355427/100‑1 (MQ=255)
                                                          gCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAAc                                    <  1:2270249/100‑1 (MQ=255)
                                                           cggACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACa                                   <  2:1116823/98‑1 (MQ=255)
                                                            tGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACAt                                  >  2:90199/1‑100 (MQ=255)
                                                            tGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACAt                                  <  2:2336084/100‑1 (MQ=255)
                                                             gACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATg                                 <  1:2064586/100‑1 (MQ=255)
                                                             gACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATg                                 <  2:1285553/100‑1 (MQ=255)
                                                               cGCTCATTTCTCAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGc                               >  1:2471841/1‑100 (MQ=255)
                                                                   cATTTCTCAACGGATGTCCCGGGGAGAGCGGAGGGAAGTTGAACTCGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAggg                           <  1:1198215/100‑1 (MQ=255)
                                                                        ctcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGATCTGGCAGACGGAAACTGGTTACTGAACAATGCGTGGTATGAAAGAAACATGGCAGGGTTTaa                      >  2:3963028/1‑100 (MQ=255)
                                                                         tcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAAc                     >  1:1418074/1‑100 (MQ=255)
                                                                         tcAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAAc                     <  2:2086444/100‑1 (MQ=255)
                                                                          cAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACg                    <  1:3041319/100‑1 (MQ=255)
                                                                          cAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACg                    <  1:2829020/100‑1 (MQ=255)
                                                                          cAACGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACg                    <  2:3705171/100‑1 (MQ=255)
                                                                           aaCGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAAGTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGa                   >  1:3878973/1‑100 (MQ=255)
                                                                           aaCGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGa                   >  1:3352217/1‑100 (MQ=255)
                                                                           aaCGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGa                   >  2:1765650/1‑100 (MQ=255)
                                                                           aaCGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGa                   >  1:1496114/1‑100 (MQ=255)
                                                                             cGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAAc                 >  1:2716343/1‑100 (MQ=255)
                                                                             cGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAAc                 <  1:2430207/100‑1 (MQ=255)
                                                                             cGGATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAAc                 <  1:1782097/100‑1 (MQ=255)
                                                                              ggATTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACa                >  2:1307504/1‑100 (MQ=255)
                                                                                aTTTCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGt              >  2:2031569/1‑100 (MQ=255)
                                                                                 tttCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGtt             <  2:3477523/100‑1 (MQ=255)
                                                                                  ttCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTg            <  2:1959380/100‑1 (MQ=255)
                                                                                   tCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGa           >  1:1085110/1‑100 (MQ=255)
                                                                                   tCCCGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGa           <  1:2148394/100‑1 (MQ=255)
                                                                                    cccGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGaa          <  2:2758551/100‑1 (MQ=255)
                                                                                    cccGGGGAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGaa          >  2:3183458/1‑100 (MQ=255)
                                                                                      gggggTGAGCGGATGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACACTGCCTGGTATGAAAGAAACATGGCAGGGTTTAGCGAACAGTTGAAag        <  2:3728637/99‑1 (MQ=255)
                                                                                       ggggAGAGCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAaga       >  1:1071301/1‑100 (MQ=255)
                                                                                            gagCGGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAAcc  >  1:1110369/1‑100 (MQ=255)
                                                                                                |                                                                                               
GGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTTGAACTGGCAGACGGAAACTGGTTACTGAACAATGCCTGGTATGAAAGAAACATGGCAGGGTTTAACGAACAGTTGAAAGAGAACC  >  NC_000913/2075416‑2075607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A