Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,029	A→G	M100T (ATG→ACG)	wcaN ←	putative regulatory subunit for GalU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,029	0	A	G	99.5%	720.3 / ‑4.8	201	M100T (ATG→ACG)	wcaN	putative regulatory subunit for GalU
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (93/106); total (94/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 201 total aligned reads displayed.

TCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGT  >  NC_000913/2113933‑2114125

 cTGGCATTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAATGGTTCGCCCTGACGCACGTTCGTAAt                                                                                              <  2:1056823/100‑1 (MQ=255)

  tGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATg                                                                                             <  1:3334371/100‑1 (MQ=255)

   ggCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTGGCGCACACAAAATGAACTAGCCTACACCTAAAGGTTCGCTCTGACGCACGTTTGTAATgg                                                                                            >  2:1944138/1‑100 (MQ=255)

   ggCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATgg                                                                                            <  2:4122230/100‑1 (MQ=255)

   ggCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATgg                                                                                            >  2:4674793/1‑100 (MQ=255)

     cAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTc                                                                                          >  2:4349072/1‑100 (MQ=255)

     cAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTc                                                                                          >  2:4541755/1‑100 (MQ=255)

       gTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCAc                                                                                        <  1:630055/100‑1 (MQ=255)

       gTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCAc                                                                                        >  1:5501048/1‑100 (MQ=255)

       gTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCAc                                                                                        >  1:3462312/1‑100 (MQ=255)

        tACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACg                                                                                       <  1:4498040/100‑1 (MQ=255)

        tACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACg                                                                                       <  1:408644/100‑1 (MQ=255)

          ccACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTTACGcc                                                                                     <  2:4832562/100‑1 (MQ=255)

             cGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCcgg                                                                                  >  1:2590658/1‑100 (MQ=255)

             cGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCcgg                                                                                  >  1:1629910/1‑100 (MQ=255)

               aCAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCcggcg                                                                                <  2:3823265/100‑1 (MQ=255)

                cAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCcggcgg                                                                               >  2:3084186/1‑100 (MQ=255)

                cAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCcggcgg                                                                               <  1:2319226/100‑1 (MQ=255)

                cAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCcggcgg                                                                               <  2:5249079/100‑1 (MQ=255)

                  aaTGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGAc                                                                             >  1:3120590/1‑100 (MQ=255)

                  aaTGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGAc                                                                             >  2:4392625/1‑100 (MQ=255)

                  aaTGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGAc                                                                             <  2:3646199/100‑1 (MQ=255)

                     gggTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGa                                                                          <  2:3534210/100‑1 (MQ=255)

                       gTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATg                                                                        >  1:1405413/1‑100 (MQ=255)

                          gTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGAc                                                                     >  2:1854889/1‑100 (MQ=255)

                          gTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGAc                                                                     >  2:2042389/1‑100 (MQ=255)

                          gTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGAc                                                                     <  1:1335736/100‑1 (MQ=255)

                           tCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACt                                                                    <  1:3219858/100‑1 (MQ=255)

                           tCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACt                                                                    <  2:327349/100‑1 (MQ=255)

                           tCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACt                                                                    >  2:2410042/1‑100 (MQ=255)

                            cACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTg                                                                   >  2:952115/1‑100 (MQ=255)

                            cACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTg                                                                   >  2:1555891/1‑100 (MQ=255)

                              ccAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTa                                                                 >  1:2179594/1‑100 (MQ=255)

                              ccAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTa                                                                 >  2:807212/1‑100 (MQ=255)

                               cAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTAc                                                                <  1:4897385/100‑1 (MQ=255)

                                  tGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTc                                                             <  2:4091827/100‑1 (MQ=255)

                                  tGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTc                                                             <  1:1452734/100‑1 (MQ=255)

                                  tGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTc                                                             <  2:5320938/100‑1 (MQ=255)

                                    gCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCg                                                           <  1:4309656/100‑1 (MQ=255)

                                    gCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCg                                                           <  1:4247817/100‑1 (MQ=255)

                                     cAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGc                                                          >  2:4484756/1‑100 (MQ=255)

                                      aGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGcc                                                         <  1:1678776/100‑1 (MQ=255)

                                       ggTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCca                                                        >  2:2362585/1‑100 (MQ=255)

                                        gTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcag                                                       >  1:1165803/1‑100 (MQ=255)

                                        gTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcag                                                       >  1:1853891/1‑100 (MQ=255)

                                         tCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcagc                                                      <  2:2724471/100‑1 (MQ=255)

                                              cacacaAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTg                                                 >  1:3943331/1‑100 (MQ=255)

                                                cacaAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGAc                                               <  2:1439986/100‑1 (MQ=255)

                                                cacaAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGAc                                               >  1:5349491/1‑100 (MQ=255)

                                                cacaAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGAc                                               <  1:5144039/100‑1 (MQ=255)

                                                     aaTGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTc                                          <  2:2125437/100‑1 (MQ=255)

                                                      aTGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCa                                         <  1:2719601/100‑1 (MQ=255)

                                                       tGGAGTGGCCTAAGCCTCAAGGGGCGCCCTGACGCACGTTCGTAATGGTGACGACCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAc                                        <  2:2563923/100‑1 (MQ=255)

                                                       tGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAc                                        <  2:786622/100‑1 (MQ=255)

                                                        ggAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAcg                                       <  2:1167474/100‑1 (MQ=255)

                                                         gAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAcgc                                      <  1:2398554/100‑1 (MQ=255)

                                                          aGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAcgcg                                     <  1:4224312/100‑1 (MQ=255)

                                                           gTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACgcgc                                    >  2:2189502/1‑100 (MQ=255)

                                                            tGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCgct                                   <  2:1832491/100‑1 (MQ=255)

                                                            tGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCACCAGCAGTTGACGCTTCACGCgct                                   >  1:500662/1‑100 (MQ=255)

                                                             ggCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCgctg                                  >  2:2346377/1‑100 (MQ=255)

                                                             ggCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCgctg                                  >  2:2889981/1‑100 (MQ=255)

                                                              gCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCgctgc                                 >  2:1709486/1‑100 (MQ=255)

                                                               cctaaacctaaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTACGCCAGCAGTTGACGCTTCACGCgctgct                                >  2:3113806/1‑100 (MQ=255)

                                                                  aaacctaaaGGTTCGCCCTGATTCACGTTCTTAATGGTCACGCTCGGCGGACAGATGGACTGTACTTGCGCCAGCAGTTGACGCTTCACGCGCTGCTCaa                             >  2:1631724/1‑100 (MQ=255)

                                                                    acctaaaGCTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAgg                           <  1:4460736/100‑1 (MQ=255)

                                                                     cctaaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGa                          >  1:3506185/1‑100 (MQ=255)

                                                                     cctaaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGa                          >  2:1376573/1‑100 (MQ=255)

                                                                      gatctggTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAg                         <  2:4336215/95‑1 (MQ=255)

                                                                      ctaaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAg                         >  2:933643/1‑100 (MQ=255)

                                                                       taaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGt                        >  2:4656482/1‑100 (MQ=255)

                                                                       taaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGt                        <  1:3882670/100‑1 (MQ=255)

                                                                        aaaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTg                       <  1:5426019/100‑1 (MQ=255)

                                                                         aaGGTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGa                      <  1:4689101/100‑1 (MQ=255)

                                                                           ggTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGAtt                    >  1:5229931/1‑100 (MQ=255)

                                                                            gTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                   >  1:2160410/1‑100 (MQ=255)

                                                                            gTTCGCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                   <  1:736071/100‑1 (MQ=255)

                                                                            gTTCGCACTGACGACCGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                   <  1:255860/100‑1 (MQ=255)

                                                                                gCCCTGACGCACTTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGCGTGATTCTagc               <  2:5094975/100‑3 (MQ=255)

                                                                                gCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAAc               >  1:3795835/1‑100 (MQ=255)

                                                                                gCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAAc               <  1:4277196/100‑1 (MQ=255)

                                                                                gCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAAc               <  1:817809/100‑1 (MQ=255)

                                                                                gCCCTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAAc               <  1:837763/100‑1 (MQ=255)

                                                                                 cccTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACt              >  2:2067577/1‑100 (MQ=255)

                                                                                   cTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCa            >  1:1711599/1‑100 (MQ=255)

                                                                                   cTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCa            <  2:1592591/100‑1 (MQ=255)

                                                                                   cTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCa            >  2:5218301/1‑100 (MQ=255)

                                                                                   cTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCa            >  1:5574712/1‑100 (MQ=255)

                                                                                   cTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCa            >  1:4860255/1‑100 (MQ=255)

                                                                                   cTGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCa            >  1:4518130/1‑100 (MQ=255)

                                                                                    tGACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCAt           <  2:4461646/100‑1 (MQ=255)

                                                                                     gACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATa          <  2:550549/100‑1 (MQ=255)

                                                                                     gACGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATa          <  2:850700/100‑1 (MQ=255)

                                                                                      aCGCACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATaa         <  1:2873848/100‑1 (MQ=255)

                                                                                        gCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAaga       <  1:2171007/100‑1 (MQ=255)

                                                                                         cACGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAagag      >  2:4749423/1‑100 (MQ=255)

                                                                                           cGTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGgt    <  1:100340/100‑1 (MQ=255)

                                                                                            gTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGgtg   <  2:610858/100‑1 (MQ=255)

                                                                                            gTTCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGgtg   <  2:1787112/100‑1 (MQ=255)

                                                                                             ttCGTAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCTCGCGCTGCTCAAGGAGTGATTCTAACTCATAAGCGgtgt  <  2:4222236/100‑1 (MQ=255)

TCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGT  >  NC_000913/2113933‑2114125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A