Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,125,822 A→C L134R (CTG→CGG)  wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,125,8220AC97.4% 669.1 / 1.0 191L134R (CTG→CGG) wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (93/93);  total (95/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCAC  >  NC_000913/2125726‑2125916
                                                                                                |                                                                                              
gCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCcgcg                                                                                             >  2:3950877/1‑100 (MQ=255)
   gTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGt                                                                                          >  2:1003918/1‑100 (MQ=255)
     aGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTcc                                                                                        >  2:871835/1‑100 (MQ=255)
     aGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTcc                                                                                        >  1:5409546/1‑100 (MQ=255)
      gACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCg                                                                                       <  1:4578008/100‑1 (MQ=255)
        ccAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGAc                                                                                     <  1:3948311/100‑1 (MQ=255)
        ccAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGAc                                                                                     >  2:1048171/1‑100 (MQ=255)
         cAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACg                                                                                    <  1:1874937/100‑1 (MQ=255)
         cAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACg                                                                                    >  2:1694834/1‑100 (MQ=255)
         cAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACg                                                                                    >  1:3199213/1‑100 (MQ=255)
         cAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACg                                                                                    <  2:4380319/100‑1 (MQ=255)
          aGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGt                                                                                   >  1:3019796/1‑100 (MQ=255)
           gTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTc                                                                                  >  1:2831382/1‑100 (MQ=255)
             aTTTTCATCATCCGGGGCCTCCAGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTGGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGGcag                                                                                <  2:517371/100‑1 (MQ=255)
             aTTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcag                                                                                <  2:1754531/100‑1 (MQ=255)
             aTTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcag                                                                                <  1:438792/100‑1 (MQ=255)
              ttttCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagc                                                                               >  1:3278156/1‑100 (MQ=255)
               tttCATAATCCGTGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTCTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagcc                                                                              <  1:5271453/100‑1 (MQ=255)
               tttCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagcc                                                                              >  2:419708/1‑100 (MQ=255)
               tttCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagcc                                                                              >  1:5192841/1‑100 (MQ=255)
                ttCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagcca                                                                             >  2:4817701/1‑100 (MQ=255)
                ttCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagcca                                                                             <  2:695117/100‑1 (MQ=255)
                 tCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTcagccag                                                                            >  2:2391490/1‑100 (MQ=255)
                   aTAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCg                                                                          >  1:3312101/1‑100 (MQ=255)
                    tAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCgg                                                                         <  1:439748/100‑1 (MQ=255)
                    tAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCgg                                                                         >  2:110527/1‑100 (MQ=255)
                    naaTCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCgg                                                                         >  1:2708058/2‑100 (MQ=255)
                         cGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATc                                                                    >  2:2957524/1‑100 (MQ=255)
                         cGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATc                                                                    >  1:154424/1‑100 (MQ=255)
                           ggTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGt                                                                  <  1:1447748/100‑1 (MQ=255)
                           ggTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGt                                                                  <  2:47484/100‑1 (MQ=255)
                             tACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCa                                                                <  1:5017109/100‑1 (MQ=255)
                             tACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCa                                                                <  1:3768957/100‑1 (MQ=255)
                             tACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCa                                                                <  2:3475089/100‑1 (MQ=255)
                             tACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCa                                                                >  1:2605818/1‑100 (MQ=255)
                              aCTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAt                                                               <  2:4015989/100‑1 (MQ=255)
                               cTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtg                                                              >  1:2459820/1‑100 (MQ=255)
                                tCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgc                                                             <  1:4199809/100‑1 (MQ=255)
                                 ccGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgct                                                            <  2:941998/100‑1 (MQ=255)
                                    gTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATgctgct                                                         <  1:3718880/100‑1 (MQ=255)
                                     tACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTc                                                        <  1:2951851/100‑1 (MQ=255)
                                        gCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATc                                                     >  1:4596580/1‑100 (MQ=255)
                                        gCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATc                                                     <  2:5454551/100‑1 (MQ=255)
                                         cTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATcc                                                    >  1:1043383/1‑100 (MQ=255)
                                          ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCg                                                   <  2:3576391/100‑1 (MQ=255)
                                             tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa                                                <  1:4930031/100‑1 (MQ=255)
                                              cAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAg                                               <  2:2126863/100‑1 (MQ=255)
                                               aGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGt                                              <  2:373983/100‑1 (MQ=255)
                                                gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTa                                             >  1:3562551/1‑100 (MQ=255)
                                                 cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTag                                            >  2:4971292/1‑99 (MQ=255)
                                                  gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAAc                                           >  2:4491461/1‑100 (MQ=255)
                                                  gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAAc                                           >  1:3879454/1‑100 (MQ=255)
                                                  gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAAc                                           >  1:2810090/1‑100 (MQ=255)
                                                   agaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACa                                          <  1:1714787/100‑1 (MQ=255)
                                                     aaaaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc                                        <  1:4498209/100‑1 (MQ=255)
                                                     aaaaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc                                        <  2:3885484/100‑1 (MQ=255)
                                                       aaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGctc                                      <  1:1699071/100‑1 (MQ=255)
                                                       aaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCGGCTCATCCGGCAGTAACAGctc                                      <  1:4656083/100‑1 (MQ=255)
                                                          aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCtcttc                                   >  2:1830634/1‑100 (MQ=255)
                                                           ggCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCtcttct                                  >  2:4272876/1‑100 (MQ=255)
                                                           ggCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCtattct                                  >  2:5077773/1‑100 (MQ=255)
                                                             cgcgGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCttcttc                                <  1:2501880/100‑1 (MQ=255)
                                                              gcgGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTcc                               <  2:3281354/100‑1 (MQ=255)
                                                              gcgGCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTcc                               <  1:1017287/100‑1 (MQ=255)
                                                                ggCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                             >  1:1181476/1‑100 (MQ=255)
                                                                ggCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                             <  2:1369428/100‑1 (MQ=255)
                                                                 gCTGTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGAt                            <  2:2302034/100‑1 (MQ=255)
                                                                  cTGTTAGCATGAACGTTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                           >  2:1622404/1‑100 (MQ=255)
                                                                  cTGTTAGCATGAACATTGTCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                           <  1:2348713/100‑1 (MQ=255)
                                                                    gTTAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                         >  1:3978279/1‑100 (MQ=255)
                                                                     ttAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActc                        <  2:591406/100‑1 (MQ=255)
                                                                      tAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGTGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActct                       >  1:1358243/1‑100 (MQ=255)
                                                                      tAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActct                       <  1:848391/100‑1 (MQ=255)
                                                                      tAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActct                       <  1:3401994/100‑1 (MQ=255)
                                                                      tAGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActct                       >  1:5536387/1‑100 (MQ=255)
                                                                       aGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTg                      >  2:2053879/1‑100 (MQ=255)
                                                                       aGCATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTg                      >  2:3968808/1‑100 (MQ=255)
                                                                         cATGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGaa                    >  1:2244390/1‑100 (MQ=255)
                                                                          aTGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAg                   >  1:881719/1‑100 (MQ=255)
                                                                           tGAACGTTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                  <  1:1642051/100‑1 (MQ=255)
                                                                           tGAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                  >  1:5225143/1‑100 (MQ=255)
                                                                            gAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCg                 >  1:4095544/1‑100 (MQ=255)
                                                                            gAACATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCg                 >  2:5376024/1‑100 (MQ=255)
                                                                             aaCATTATCACTGGCAAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCCGTCACAGCCCTTCTTCCGATACTCTGAAGGGa                >  2:381903/1‑100 (MQ=255)
                                                                               cATTATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa              <  1:4327595/100‑1 (MQ=255)
                                                                                 ttATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc            >  1:608116/1‑100 (MQ=255)
                                                                                 ttATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc            <  1:894932/100‑1 (MQ=255)
                                                                                 ttATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc            >  1:206090/1‑100 (MQ=255)
                                                                                 ttATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc            >  2:1870507/1‑100 (MQ=255)
                                                                                 ttATCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc            <  2:2891058/100‑1 (MQ=255)
                                                                                   aTCACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCg          >  1:3614158/1‑100 (MQ=255)
                                                                                     cACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg        >  2:5104336/1‑100 (MQ=255)
                                                                                     cACTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCGTCTTCCGATACTCTGAAGCGAAAACCGAg        >  1:5079879/1‑100 (MQ=255)
                                                                                      aCTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc       >  2:1205082/1‑100 (MQ=255)
                                                                                      aCTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc       >  2:2609893/1‑100 (MQ=255)
                                                                                       cTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca      <  1:5493296/100‑1 (MQ=255)
                                                                                       cTGGCGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca      >  2:227301/1‑100 (MQ=255)
                                                                                           cGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcaccac  <  1:2672512/100‑1 (MQ=255)
                                                                                           cGAGCCGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcaccac  >  2:2679765/1‑100 (MQ=255)
                                                                                           cGAGCCGCGCGTCCGACGTCACCCAGCGGTAATCGTCATGCTGCCCATCCGCCAGCAACAGCTCTTCTTCCGCTACTCCGAAGCGAAAACCcaccaccac  >  2:4715236/1‑100 (MQ=255)
                                                                                                |                                                                                              
GCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCAC  >  NC_000913/2125726‑2125916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A