Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,127,751 T→G E188D (GAA→GAC)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,127,7510TG97.5% 261.7 / ‑4.2 81E188D (GAA→GAC) gmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (38/40);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGC  >  NC_000913/2127520‑2127982
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
cATTTCCACGATCTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCa                                                                                                                                                                                                                                       <  1:765957/235‑1 (MQ=255)
cATTTCCACGAAGTGCCGGAAGGACTACTGACCGCCGTCCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCa                                                                                                                                                                                                                                       <  2:323482/235‑1 (MQ=255)
   ttCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGCGATTTTGCGGGGAACGAAGGATTCGCCACGGAGCGGGGAGTCAT‑GGt                                                                                                                                                                                                                                  >  2:391432/1‑236 (MQ=255)
    tCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGtt                                                                                                                                                                                                                                 <  1:93459/236‑1 (MQ=255)
      cACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGa                                                                                                                                                                                                                               >  1:669346/1‑236 (MQ=255)
       aggAACTGACGCCCGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGaa                                                                                                                                                                                                                              <  1:187464/234‑1 (MQ=255)
       aCGAACTGACGCAAGGAGTACGGACCGCCGGTCGCGATAACGAAAGCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCGTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGaa                                                                                                                                                                                                                              <  2:127153/236‑1 (MQ=255)
         gatcTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAaga                                                                                                                                                                                                                            <  1:765636/233‑1 (MQ=255)
               aCGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTc                                                                                                                                                                                                                      >  1:136561/1‑236 (MQ=255)
               aCGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTc                                                                                                                                                                                                                      >  2:249124/1‑236 (MQ=255)
                    cggaagaCTGAACCTCGTTCGCGTACACGAAATCTTCCGGCTTTTCCTGCTGCAACATCACCCACTGCATTGTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTa                                                                                                                                                                                                                 <  2:226339/230‑1 (MQ=255)
                    cGGAGTACTGAACGCCGTTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTa                                                                                                                                                                                                                 <  1:740265/236‑1 (MQ=255)
                    aggAATACTGAACGCCGGTCGCGATAACGAATGCTTCCGGCTGGTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTa                                                                                                                                                                                                                 <  2:201221/235‑1 (MQ=255)
                      gAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACa                                                                                                                                                                                                               <  1:235039/236‑1 (MQ=255)
                          aCTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGGGATTTTGCGGGTAACGAAGGGTTCGCCGCGGCGCGGGGA‑GCATGGGTTTAAGAGAATTCCGtttaatgcg                                                                                                                                                                                                           >  2:137151/1‑229 (MQ=255)
                             gaccGCCGGTCGAGAAACCGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTAc                                                                                                                                                                                                        <  2:669346/233‑1 (MQ=255)
                              aaCGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACa                                                                                                                                                                                                       <  1:451119/236‑1 (MQ=255)
                                 gCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGc                                                                                                                                                                                                    >  1:579837/1‑236 (MQ=255)
                                   cGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGGGATTTTTCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCCT‑GGTTGAAGAGAATTCCGTGAAAGGCGTACATGCCg                                                                                                                                                                                                  >  2:200363/1‑236 (MQ=255)
                                             aaCGAATTCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTCCGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                                                                                                         <  1:371249/235‑1 (MQ=255)
                                             aaCGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                                                                                                                                                                        <  1:249124/236‑1 (MQ=255)
                                                   aTCTTCCGGCTCTTCCGGCTGCAGCTTCAGCCACGGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCAATATTGCCGAGGTACGGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGt                                                                                                                                                                                  <  2:579837/236‑1 (MQ=255)
                                                         cGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGGACAGTCCGTAGGATTTACGGTAGTTAACg                                                                                                                                                                             >  2:40408/1‑235 (MQ=255)
                                                                 ccTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                                                                                                                                                                    <  1:393438/236‑1 (MQ=255)
                                                                                tcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATTTTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGttt                                                                                                                                                      >  2:711023/1‑235 (MQ=255)
                                                                                tcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTg                                                                                                                                                     >  1:618081/1‑236 (MQ=255)
                                                                                tcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACAGGCCGTAGGATTGACGGTAGTTAACGGTGATCCAGTAGCCGTACAGTTTg                                                                                                                                                     >  2:739357/1‑236 (MQ=255)
                                                                                     tGCATTGTTACGTAATCTTTGGCCGGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAc                                                                                                                                                <  2:693680/236‑1 (MQ=255)
                                                                                                 tAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGgaga                                                                                                                                    >  1:678233/1‑236 (MQ=255)
                                                                                                      tttGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATcgc                                                                                                                                <  2:678233/235‑1 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                                                                                                <  1:556514/163‑1 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                                                                                                >  2:556514/1‑163 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGt                                                                                                                                                                           >  2:83922/1‑184 (MQ=255)
                                                                                                              ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGt                                                                                                                                                                           <  1:83922/184‑1 (MQ=255)
                                                                                                                          gCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACggac                                                                                                                   >  1:363079/1‑226 (MQ=255)
                                                                                                                          gCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTACCGGTAGTTAACGGTGATCCAGTAGGCGTACAGGTTGGCGAACGCATACGGAGATCTCGGGTAGAACGGCGTggagt                                                                                                             >  2:158409/1‑231 (MQ=255)
                                                                                                                              ggaaccctgtttGCCGAGGTCCAGGCCCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCt                                                                                                       <  2:136561/226‑1 (MQ=255)
                                                                                                                                  tCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGTGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCg                                                                                                    <  1:200363/235‑1 (MQ=255)
                                                                                                                                        ttGCCGAGGTACAGGCACGACCCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGGGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGCTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGaa                                                                                                 >  1:197814/1‑232 (MQ=255)
                                                                                                                                         tGCCGAGTTAAAGGCACGACCCCAGCCCGTGGGCGATGTTGGCGATGGCGCGGGTGATTTGGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa                                                                                                  <  2:575351/230‑1 (MQ=255)
                                                                                                                                           ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTGTCTTTCTGCGGCATTTccg                                                                                           >  2:641651/1‑234 (MQ=255)
                                                                                                                                            cGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGCGATCGCGGGAAGAACGGCGGGGTTCCTTTTTGCGGAATTGCCTGc                                                                                         >  1:340178/1‑236 (MQ=255)
                                                                                                                                             gAGGTACGGGCCCGACTCCAGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:566527/236‑1 (MQ=255)
                                                                                                                                             gAGGTACAGGCACGACTCCAGCCCTTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:641651/236‑1 (MQ=255)
                                                                                                                                             gAGGTACAGGCACGACTCCAGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:137151/236‑1 (MQ=255)
                                                                                                                                              aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        >  1:287093/1‑235 (MQ=255)
                                                                                                                                              aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGGTTGGCGACCGCATACGGCGATCGCGGGTAGAACGGCGTGGTCTCGTTCTGCGGAATTTCCCGCa                                                                                        >  2:24182/1‑235 (MQ=255)
                                                                                                                                                gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCt                                                                                           >  1:575351/1‑230 (MQ=255)
                                                                                                                                                   cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        <  1:301557/230‑1 (MQ=255)
                                                                                                                                                   cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCa                                                                                        >  2:301557/1‑230 (MQ=255)
                                                                                                                                                        aCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc                                                                                                              >  2:489663/1‑203 (MQ=255)
                                                                                                                                                        aCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc                                                                                                              <  1:489663/203‑1 (MQ=255)
                                                                                                                                                          gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGGGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa                                                                              <  2:545234/233‑1 (MQ=255)
                                                                                                                                                          gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAg                                                                           >  1:456483/1‑236 (MQ=255)
                                                                                                                                                           tcTCCAGCCCCTGGGTGATGTTGGCGATGGGGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                                             <  2:256925/216‑1 (MQ=255)
                                                                                                                                                           aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                                                                                                         <  2:649088/125‑1 (MQ=255)
                                                                                                                                                           aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                                                                                                         >  1:649088/1‑125 (MQ=255)
                                                                                                                                                           aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGt                                                                          >  1:604613/1‑236 (MQ=255)
                                                                                                                                                                  ccccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAcc                                                                                      >  1:256925/1‑217 (MQ=255)
                                                                                                                                                                    ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTg                                                                 >  1:101908/1‑236 (MQ=255)
                                                                                                                                                                      tGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGt                                                                  <  2:618081/233‑1 (MQ=255)
                                                                                                                                                                       gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACa                                                                                                                                                                                                               >  2:220871/1‑91 (MQ=255)
                                                                                                                                                                       gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACa                                                                                                                                                                                                               <  1:220871/91‑1 (MQ=255)
                                                                                                                                                                       gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGaa                                                              >  1:762707/1‑236 (MQ=255)
                                                                                                                                                                           gaggTTGACGATGGCGCGGGTGATTTGGCGGGTAACGAAGGTTGCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTg                                                                 <  2:545885/226‑1 (MQ=255)
                                                                                                                                                                            aTGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGCTCCAGTAGGCGTACAGTTTGGCGACCGCATACGGGGATCGCGGGTAGAACGGCGTGGCATCTTCCTGCGGCATTTCCTGCACCCAACCATACAGTTCGTAGGTGCAAGCCTg                                                         >  2:72644/1‑236 (MQ=255)
                                                                                                                                                                               ttGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATa                                                      >  1:430830/1‑236 (MQ=255)
                                                                                                                                                                                tGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACCGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTTCGGAATTTCCTGCGCCAGACCATACAGTTCAGAGGGGGAAGCCTGTTa                                                      >  2:332401/1‑235 (MQ=255)
                                                                                                                                                                                  gcaaTTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGaa                                                   <  1:391432/233‑1 (MQ=255)
                                                                                                                                                                                  gCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGATGTGGAAGCCt                                                          >  1:545885/1‑229 (MQ=255)
                                                                                                                                                                                       tGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAg                                              >  1:247664/1‑236 (MQ=255)
                                                                                                                                                                                           cgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa                                                                                                  <  2:636824/180‑1 (MQ=255)
                                                                                                                                                                                           cgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGa                                                                                                  >  1:636824/1‑180 (MQ=255)
                                                                                                                                                                                                        gggTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACgg                                                                                                                                       <  2:207489/130‑1 (MQ=255)
                                                                                                                                                                                                        gggTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACgg                                                                                                                                       >  1:207489/1‑130 (MQ=255)
                                                                                                                                                                                                                          cgcggcgcggGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                                             <  2:234456/154‑1 (MQ=255)
                                                                                                                                                                                                                          cgcggcgcggGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTc                                                                                             >  1:234456/1‑154 (MQ=255)
                                                                                                                                                                                                                             ggcgcggGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATc                                                                                                                                  >  2:312295/1‑114 (MQ=255)
                                                                                                                                                                                                                             ggcgcggGGAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATc                                                                                                                                  <  1:312295/114‑1 (MQ=255)
                                                                                                                                                                                                                                   gggAGTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGgcgc  <  2:604613/236‑1 (MQ=255)
                                                                                                                                                                                                                                    gtaTTCAT‑GGTTGACGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGgcgc  <  2:363079/233‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
CATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCAT‑GGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGC  >  NC_000913/2127520‑2127982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A