Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,127,751 T→G E188D (GAA→GAC)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,127,7510TG97.0% 678.4 / 2.7 197E188D (GAA→GAC) gmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base G (81/110);  total (84/113)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

ATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCAT  >  NC_000913/2127655‑2127845
                                                                                                |                                                                                              
atTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCa                                                                                             <  1:2845635/100‑1 (MQ=255)
  tGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATg                                                                                           <  1:1381696/100‑1 (MQ=255)
  tGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATg                                                                                           <  2:3179824/100‑1 (MQ=255)
    ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGt                                                                                         >  2:3622537/1‑100 (MQ=255)
     cGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGtt                                                                                        <  2:2098939/100‑1 (MQ=255)
         gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAag                                                                                    <  1:729420/100‑1 (MQ=255)
          tACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAaga                                                                                   >  1:1023293/1‑100 (MQ=255)
            cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAgaga                                                                                 >  2:4577935/1‑100 (MQ=255)
             aGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGaa                                                                                >  1:3918042/1‑100 (MQ=255)
             aGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGaa                                                                                <  1:2905535/100‑1 (MQ=255)
             aGGCACGACTCCAGCCCCTGGGCGACGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGaa                                                                                >  2:3311314/1‑100 (MQ=255)
              ggCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGTGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAt                                                                               >  2:4717196/1‑100 (MQ=255)
              ggCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAt                                                                               >  2:3382859/1‑100 (MQ=255)
              ggCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAt                                                                               >  2:2261343/1‑100 (MQ=255)
               gCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAtt                                                                              >  1:5562847/1‑100 (MQ=255)
                cACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTc                                                                             >  1:3961317/1‑100 (MQ=255)
                  cGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCg                                                                           <  1:3974451/100‑1 (MQ=255)
                  cGACGCCAGCCCCTGTGCGATGTTGGCGATTGCGCTGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGATGAGAATTCCg                                                                           <  2:5063304/100‑1 (MQ=255)
                   gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGt                                                                          <  1:1517802/100‑1 (MQ=255)
                   gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGt                                                                          <  2:4948340/100‑1 (MQ=255)
                   gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGt                                                                          <  2:2856033/100‑1 (MQ=255)
                    aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGtt                                                                         <  2:2599492/100‑1 (MQ=255)
                    aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGtt                                                                         >  2:2430308/1‑100 (MQ=255)
                     cTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTa                                                                        >  1:267801/1‑100 (MQ=255)
                     cTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTa                                                                        <  1:12623/100‑1 (MQ=255)
                     cTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTa                                                                        <  2:1874444/100‑1 (MQ=255)
                      tCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTAc                                                                       <  2:3075637/100‑1 (MQ=255)
                      tCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCCCGGCGCGGGGAGTCATTGTTGAAGAGAATTCCGTTat                                                                       <  1:3193383/100‑2 (MQ=255)
                       ccAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACa                                                                      <  1:2510649/100‑1 (MQ=255)
                        cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAg                                                                     >  1:3744403/1‑100 (MQ=255)
                         aGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAgg                                                                    <  2:4846664/100‑1 (MQ=255)
                          gCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGc                                                                   <  1:4573658/100‑1 (MQ=255)
                          gCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGc                                                                   >  2:877281/1‑100 (MQ=255)
                           ccccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCg                                                                  <  2:4096044/100‑1 (MQ=255)
                            cccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCAGCGCGGGGGGTCATGGTTGAAGAGAATTCCGTTACAGGCGt                                                                 <  2:1502961/100‑1 (MQ=255)
                             ccTGGGCTATGGTGGCGATTGCGCGGGGGATTTTGCTGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                <  2:4591216/100‑1 (MQ=255)
                             ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                <  1:4509361/100‑1 (MQ=255)
                             ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                >  1:4633282/1‑100 (MQ=255)
                             ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                <  2:2399648/100‑1 (MQ=255)
                               tcggCGATGTTGGCGCCTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACCGGCGTACa                                                              <  2:1911286/98‑1 (MQ=255)
                               tGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                              <  1:365028/100‑1 (MQ=255)
                               ggggCGATGGTGGCGGTGTCGCGGCGGATTTTGCGGGGATTGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGACTTCCGTTACGGGCGTACa                                                              <  2:5388369/99‑1 (MQ=255)
                                gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACAt                                                             <  2:4260043/100‑1 (MQ=255)
                                 ggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATg                                                            <  2:3365518/100‑1 (MQ=255)
                                 ggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATg                                                            >  2:2710769/1‑100 (MQ=255)
                                  gCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGc                                                           <  2:3730093/100‑1 (MQ=255)
                                  gCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGc                                                           <  2:4401862/100‑1 (MQ=255)
                                   cGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGcc                                                          <  1:4595244/100‑1 (MQ=255)
                                    gATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCg                                                         <  1:2062769/100‑1 (MQ=255)
                                       gTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAg                                                      >  1:676961/1‑100 (MQ=255)
                                        ttGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAgg                                                     <  2:2341641/100‑1 (MQ=255)
                                        ttGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAgg                                                     <  1:2426262/100‑1 (MQ=255)
                                          ggCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGAt                                                   <  2:1348765/100‑1 (MQ=255)
                                          ggCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGAt                                                   >  1:1807638/1‑100 (MQ=255)
                                          ggCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGAt                                                   >  2:343278/1‑100 (MQ=255)
                                          ggCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGAt                                                   <  1:3281022/100‑1 (MQ=255)
                                              aTTGCGCGGGTGATTTTGCGGGTAACGCAGGTTTCGCCGCGGCGCGGAGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                               <  1:4223590/100‑1 (MQ=255)
                                              aTTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                               <  1:2259541/100‑1 (MQ=255)
                                               ttGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                              <  1:2040372/100‑1 (MQ=255)
                                                tGCGCGAGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACgg                                             <  1:3535681/100‑1 (MQ=255)
                                                 gcgcGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGt                                            <  1:2654857/100‑1 (MQ=255)
                                                  cgcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTa                                           <  2:3573873/100‑1 (MQ=255)
                                                   gcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAg                                          <  2:2104848/100‑1 (MQ=255)
                                                   gcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAg                                          <  2:2677558/100‑1 (MQ=255)
                                                   gcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGAATTCACGGTAg                                          <  2:5330975/100‑1 (MQ=255)
                                                   gcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGGCATGGTTGAAGAGAATTCCGTTACAGGCGGACATGCCGTAGGATTCACGGTAg                                          <  1:2372042/100‑1 (MQ=255)
                                                     gggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGtt                                        >  1:1710938/1‑100 (MQ=255)
                                                     gggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGtt                                        <  1:2348804/100‑1 (MQ=255)
                                                     gggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGCAGGATTCACGGTAGtt                                        <  1:1155327/100‑1 (MQ=255)
                                                        tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAAc                                     <  2:3882260/100‑1 (MQ=255)
                                                        tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGGACATGACGTAGGATTCACGGTAGTTAAc                                     >  1:5072327/1‑100 (MQ=255)
                                                          aTTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACgg                                   >  2:296487/1‑100 (MQ=255)
                                                           ttttGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGt                                  <  1:5258617/100‑1 (MQ=255)
                                                               gCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCATAGGATTCACGGTAGTTAACGGTGATc                              >  1:3688157/1‑100 (MQ=255)
                                                                cGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATcc                             >  1:442768/1‑100 (MQ=255)
                                                                cGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATcc                             >  1:1771573/1‑100 (MQ=255)
                                                                cGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATcc                             <  2:3664984/100‑1 (MQ=255)
                                                                  ggTAACGAAGGTTTCGCCGCGGCCCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                           <  1:4185152/100‑1 (MQ=255)
                                                                   gTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTTCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGt                          <  2:4984105/100‑1 (MQ=255)
                                                                   gTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGt                          <  1:2533172/100‑1 (MQ=255)
                                                                    tAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTa                         <  1:4535691/100‑1 (MQ=255)
                                                                    tAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTa                         >  1:5398488/1‑100 (MQ=255)
                                                                     aaCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAg                        >  2:490507/1‑100 (MQ=255)
                                                                     aaCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAg                        >  1:1044990/1‑100 (MQ=255)
                                                                     aaCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAg                        >  1:91402/1‑100 (MQ=255)
                                                                      aCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAgg                       <  2:3925208/100‑1 (MQ=255)
                                                                      aCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAgg                       <  1:5191625/100‑1 (MQ=255)
                                                                      aCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAgg                       <  1:4095567/100‑1 (MQ=255)
                                                                       cGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGc                      >  1:4271734/1‑100 (MQ=255)
                                                                        gAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCg                     >  2:4475631/1‑100 (MQ=255)
                                                                          aGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCTGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTa                   >  1:2114049/1‑100 (MQ=255)
                                                                           ggTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTAc                  <  1:3342420/100‑1 (MQ=255)
                                                                             tttCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAg                >  1:1971563/1‑100 (MQ=255)
                                                                                cgccgcGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACGTGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGttt             >  1:4760401/1‑100 (MQ=255)
                                                                                cgccgcGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGttt             <  1:299296/100‑1 (MQ=255)
                                                                                  ccgcGGCGCGGCGAGTCATGGTTGAAGAGAATTCCGTTACTGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTgg           <  1:4920471/100‑1 (MQ=255)
                                                                                   cgcggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGc          >  2:1183585/1‑100 (MQ=255)
                                                                                       gcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAcc      <  1:5386585/100‑1 (MQ=255)
                                                                                        cgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCg     >  2:1049611/1‑100 (MQ=255)
                                                                                           ggggAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCAt  <  1:3574984/100‑1 (MQ=255)
                                                                                                |                                                                                              
ATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCAT  >  NC_000913/2127655‑2127845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A