Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,127,751 T→G E188D (GAA→GAC)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,127,7510TG98.0% 514.7 / ‑0.9 152E188D (GAA→GAC) gmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (71/77);  total (74/78)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 152 total aligned reads displayed.

TATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATA  >  NC_000913/2127654‑2127846
                                                                                                 |                                                                                               
tatTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTc                                                                                               <  2:778954/100‑1 (MQ=255)
   tGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAATGACGGATTCGCGGCGGCGCGCGGAGTCCTg                                                                                            >  2:1842459/1‑100 (MQ=255)
    gCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATgg                                                                                           >  2:850742/1‑100 (MQ=255)
    gCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATgg                                                                                           >  2:3509917/1‑100 (MQ=255)
     ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGt                                                                                          >  2:453734/1‑100 (MQ=255)
     ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGt                                                                                          <  2:2763976/100‑1 (MQ=255)
     ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGt                                                                                          <  2:3895847/100‑1 (MQ=255)
     ccGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGt                                                                                          <  2:2027239/100‑1 (MQ=255)
       gAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTg                                                                                        <  1:3349779/100‑1 (MQ=255)
       gAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTg                                                                                        >  2:19838/1‑100 (MQ=255)
       gAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTg                                                                                        <  2:20089/100‑1 (MQ=255)
       gAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTg                                                                                        <  1:117546/100‑1 (MQ=255)
       gAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTg                                                                                        >  1:425534/1‑100 (MQ=255)
       gAGGTACAGGCACGACTCCAGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTg                                                                                        <  1:2001992/100‑1 (MQ=255)
       gAGGTACAGGCAAGACTCCAGCCCCTGGGCGATGTTGGTGTTTGAGCGGGTGATTTTGAGGTTACCGGAGGTTTCGCCGCGGCGCGGGAAGTCATGGTTg                                                                                        <  2:1593211/100‑1 (MQ=255)
        tggTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGa                                                                                       <  2:238465/99‑1 (MQ=255)
        aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGa                                                                                       <  2:124138/100‑1 (MQ=255)
         ggTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGaa                                                                                      >  1:242341/1‑100 (MQ=255)
         ggTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGaa                                                                                      >  1:3891083/1‑100 (MQ=255)
          gTACAGGCACGCCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAag                                                                                     <  2:1645951/100‑1 (MQ=255)
          gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGACGGTTTCGCCGCGGCGCGGGGAGGCATGGTTGAag                                                                                     >  1:3459525/1‑100 (MQ=255)
           tACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAaga                                                                                    >  1:2207297/1‑100 (MQ=255)
            aCAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAagag                                                                                   >  2:1782056/1‑100 (MQ=255)
            aCAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAagag                                                                                   >  2:3976661/1‑100 (MQ=255)
            aCAGGCACGACTCCAGCCCCTGGGCGATGTGGGCGAGTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAagag                                                                                   <  1:152456/100‑1 (MQ=255)
              aGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGaa                                                                                 <  1:1385150/100‑1 (MQ=255)
              aGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGaa                                                                                 >  1:3945294/1‑100 (MQ=255)
               ggCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAt                                                                                >  2:2538934/1‑100 (MQ=255)
                gCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAtt                                                                               >  1:2473414/1‑100 (MQ=255)
                gCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAtt                                                                               >  1:4045698/1‑100 (MQ=255)
                gCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAAtt                                                                               >  2:517210/1‑100 (MQ=255)
                 cACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTc                                                                              >  1:350575/1‑100 (MQ=255)
                   cGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCg                                                                            >  1:1768913/1‑100 (MQ=255)
                   cGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCg                                                                            <  1:1250783/100‑1 (MQ=255)
                    gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGt                                                                           <  1:3295611/100‑1 (MQ=255)
                     aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGtt                                                                          >  1:1566403/1‑100 (MQ=255)
                     aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGtt                                                                          <  2:132038/100‑1 (MQ=255)
                     aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGtt                                                                          >  2:3422791/1‑100 (MQ=255)
                       tCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTAc                                                                        <  1:1018730/100‑1 (MQ=255)
                        ccAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACa                                                                       >  1:519270/1‑100 (MQ=255)
                        ccAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACa                                                                       >  1:955248/1‑100 (MQ=255)
                         cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAg                                                                      <  1:3912277/100‑1 (MQ=255)
                         cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAg                                                                      <  1:2300011/100‑1 (MQ=255)
                         cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAg                                                                      >  1:1622544/1‑100 (MQ=255)
                         cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAg                                                                      <  1:1848849/100‑1 (MQ=255)
                          aGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGGTACAgg                                                                     <  2:3479543/100‑1 (MQ=255)
                              ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                 >  1:1002550/1‑100 (MQ=255)
                              ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                 <  2:2049311/100‑1 (MQ=255)
                              ccTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTa                                                                 <  2:1837246/100‑1 (MQ=255)
                               cTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGAGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTAc                                                                >  2:2468633/1‑100 (MQ=255)
                                tGGGGGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                               <  2:3528927/100‑1 (MQ=255)
                                tGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                               >  1:2751241/1‑100 (MQ=255)
                                 gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACAt                                                              <  2:463919/100‑1 (MQ=255)
                                 gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACAt                                                              <  1:371925/100‑1 (MQ=255)
                                   gCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGc                                                            >  1:84408/1‑100 (MQ=255)
                                      aTGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGt                                                         <  1:1934790/100‑1 (MQ=255)
                                      aTGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGt                                                         <  2:3158208/100‑1 (MQ=255)
                                        gTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAg                                                       <  1:1531829/100‑1 (MQ=255)
                                         ttGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAgg                                                      <  1:2491062/100‑1 (MQ=255)
                                         ttGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCACGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAgg                                                      <  1:3766892/100‑1 (MQ=255)
                                          tGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGa                                                     >  2:2301249/1‑100 (MQ=255)
                                             cGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTc                                                  >  2:1119237/1‑100 (MQ=255)
                                             cGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTc                                                  >  2:3480819/1‑100 (MQ=255)
                                             cGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGCGAGTCATGGTTGGAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTc                                                  >  2:1859213/1‑100 (MQ=255)
                                              gATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                 >  1:1420342/1‑100 (MQ=255)
                                               aTTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCan                                                <  1:3241034/100‑2 (MQ=255)
                                               aTTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                                >  2:220085/1‑100 (MQ=255)
                                                ttGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                               <  2:1028443/100‑1 (MQ=255)
                                                ttGCGCGGGTGATTTTGCGGGGAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                               <  2:660832/100‑1 (MQ=255)
                                                  gcgcGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGt                                             <  1:650818/100‑1 (MQ=255)
                                                    gcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAg                                           <  2:73565/100‑1 (MQ=255)
                                                     cgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGt                                          >  2:2098985/1‑100 (MQ=255)
                                                       ggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTa                                        >  1:2938048/1‑100 (MQ=255)
                                                       ggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTa                                        >  1:600265/1‑100 (MQ=255)
                                                        gTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACACGCGTACATGCCGTAGGATTCACGGTAGTTaa                                       <  1:3277957/100‑1 (MQ=255)
                                                         tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAAc                                      <  2:1275795/100‑1 (MQ=255)
                                                         tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAAc                                      <  1:114125/100‑1 (MQ=255)
                                                              ttGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGa                                 >  1:842754/1‑100 (MQ=255)
                                                               tGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGAt                                <  2:2723485/100‑1 (MQ=255)
                                                               tGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGAt                                <  1:2778969/100‑1 (MQ=255)
                                                                 cGGGTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATcc                              >  1:1997220/1‑100 (MQ=255)
                                                                  gggTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCa                             >  2:913117/1‑100 (MQ=255)
                                                                  gggTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGGTGAAGAGAATTCCGTTAGAGGCGTACATGCCGTAGGATTCACGGGAGTTAACGGTGATCCa                             >  2:2827740/1‑100 (MQ=255)
                                                                    gTAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGt                           <  1:1872525/100‑1 (MQ=255)
                                                                     tAACGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTa                          <  1:2257349/100‑1 (MQ=255)
                                                                      aaCGAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAg                         >  2:290842/1‑100 (MQ=255)
                                                                         gAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCg                      <  1:1923050/100‑1 (MQ=255)
                                                                         gAAGGTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCg                      >  2:1937304/1‑100 (MQ=255)
                                                                            ggTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTAc                   >  2:115075/1‑100 (MQ=255)
                                                                            ggTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTAc                   <  2:82991/100‑1 (MQ=255)
                                                                            ggTTTCGCCGCGGCGCGGGGAGCCATGGTTGAAGAGAATTCCGTTAAAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTAc                   >  1:2120610/1‑100 (MQ=255)
                                                                             gTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACa                  >  1:1782264/1‑100 (MQ=255)
                                                                             gTTTCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACa                  >  1:2965/1‑100 (MQ=255)
                                                                              tttCGCCGCGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAg                 >  2:3189026/1‑100 (MQ=255)
                                                                                   ccgcGGCGCGGGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTgg            <  2:1395261/100‑1 (MQ=255)
                                                                                    cgcggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGc           >  2:3430564/1‑100 (MQ=255)
                                                                                      cggcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGa         <  1:1122929/100‑1 (MQ=255)
                                                                                        gcgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAcc       <  2:1419250/100‑1 (MQ=255)
                                                                                         cgcggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCg      >  2:1001413/1‑100 (MQ=255)
                                                                                           cggGGAGTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCa    >  2:1255354/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATA  >  NC_000913/2127654‑2127846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A