Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,391,940 T→C T368A (ACC→GCC)  nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,391,9400TC99.4% 609.9 / ‑4.8 174T368A (ACC→GCC) nuoMNADH:ubiquinone oxidoreductase, membrane subunit M
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (86/86);  total (87/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 174 total aligned reads displayed.

CCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCC  >  NC_000913/2391843‑2392034
                                                                                                 |                                                                                              
 cAGGCATCCCAAGCGTTGCCACTGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATg                                                                                             >  2:1711550/1‑100 (MQ=255)
 cAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGTTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATg                                                                                             >  2:3457834/1‑100 (MQ=255)
 cAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCAtn                                                                                             <  2:4192460/100‑2 (MQ=255)
 cAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATg                                                                                             >  2:1223790/1‑100 (MQ=255)
 cAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATg                                                                                             >  1:1674685/1‑100 (MQ=255)
  aGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATgg                                                                                            <  2:1107659/100‑1 (MQ=255)
  aGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATgg                                                                                            <  1:2900480/100‑1 (MQ=255)
   ggCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGa                                                                                           >  2:617184/1‑100 (MQ=255)
   ggCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGa                                                                                           <  1:1295813/100‑1 (MQ=255)
   ggCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGa                                                                                           >  1:3867035/1‑100 (MQ=255)
   ggCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATATCGCGGGCATGGa                                                                                           >  2:4302422/1‑100 (MQ=255)
   ggCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCCTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGa                                                                                           >  1:1712553/1‑100 (MQ=255)
    gCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCAGGCGCATGTCGCGGGCATGGAt                                                                                          <  1:4755948/100‑1 (MQ=255)
     cATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATa                                                                                         >  1:3579367/1‑100 (MQ=255)
     cATCCCAACCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACCGACCGCCCATCATGCGCATGTCGCGGGCATGGATa                                                                                         <  2:3311612/100‑1 (MQ=255)
      aTCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACGGCCCATCATGCGCATGTCGCGGGCATGGATAc                                                                                        >  2:1246610/1‑100 (MQ=255)
        cccAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGt                                                                                      <  1:2124380/100‑1 (MQ=255)
           acgctttGCAACCGCAAAGAACAACGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCa                                                                                   <  1:819345/95‑1 (MQ=255)
           aaGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCa                                                                                   <  1:444079/100‑1 (MQ=255)
             gCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATa                                                                                 <  2:3831647/100‑1 (MQ=255)
               gTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATaaa                                                                               >  2:4534996/1‑100 (MQ=255)
                  gCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCt                                                                            <  2:1947039/100‑1 (MQ=255)
                  gCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCt                                                                            >  1:5109177/1‑100 (MQ=255)
                  gCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCt                                                                            >  1:3593474/1‑100 (MQ=255)
                  gCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCt                                                                            >  1:3042290/1‑100 (MQ=255)
                  gCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCt                                                                            >  1:645926/1‑100 (MQ=255)
                  gCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCt                                                                            >  2:477060/1‑100 (MQ=255)
                   ccACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTg                                                                           >  2:3090802/1‑100 (MQ=255)
                   ccACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTg                                                                           <  1:3647150/100‑1 (MQ=255)
                   ccACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTg                                                                           >  2:2231249/1‑100 (MQ=255)
                     aCCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGAc                                                                         <  2:3981291/100‑1 (MQ=255)
                     aCCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGAc                                                                         <  1:5257155/100‑1 (MQ=255)
                      ccGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGAcc                                                                        <  2:5099574/100‑1 (MQ=255)
                       cGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACca                                                                       >  2:3097931/1‑100 (MQ=255)
                             gAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGa                                                                 <  2:1197003/100‑1 (MQ=255)
                              aaCAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGaa                                                                <  1:2611591/100‑1 (MQ=255)
                               aCAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAAt                                                               <  1:5512838/100‑1 (MQ=255)
                                cAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATa                                                              >  1:1165038/1‑100 (MQ=255)
                                 aGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATaa                                                             <  1:4002712/100‑1 (MQ=255)
                                  gCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATaaa                                                            >  1:1969225/1‑100 (MQ=255)
                                   cGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAAc                                                           <  1:1394818/100‑1 (MQ=255)
                                     aCAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAg                                                         <  2:1076753/100‑1 (MQ=255)
                                        gTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGAcc                                                      >  1:5262593/1‑100 (MQ=255)
                                        gTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGAcc                                                      >  2:927122/1‑100 (MQ=255)
                                        gTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGAcc                                                      <  1:2278111/100‑1 (MQ=255)
                                        gTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGAcc                                                      <  2:1459473/100‑1 (MQ=255)
                                        gTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGAcc                                                      <  1:4510107/100‑1 (MQ=255)
                                          gCTGGCAGCCATTTCATCTTGCTCCGCAGACCGCCCAGCATGCGCATGTCGCGGGCATGGATACGATCATAAAGCTGACCACAAAGAATAAACAGACccg                                                    <  1:5176844/100‑1 (MQ=255)
                                          gCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCCGGCATGGATACGTTCATAAAGCTGACCACAAAGACTAAACAGTCccg                                                    >  2:3237230/1‑100 (MQ=255)
                                           cTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACccgc                                                   >  2:1218265/1‑100 (MQ=255)
                                           cTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACccgc                                                   >  2:2972939/1‑100 (MQ=255)
                                             ggCAGCCATTTCATCTTGCTCCACAGACCGCCCATAACGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACccgccg                                                 >  2:5370734/1‑100 (MQ=255)
                                                  ccATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGAc                                            <  2:1789037/100‑1 (MQ=255)
                                                   cATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCACCGCCGACa                                           >  2:5135876/1‑100 (MQ=255)
                                                    aTTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACaa                                          <  1:1235038/100‑1 (MQ=255)
                                                    aTTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACaa                                          >  1:1506085/1‑100 (MQ=255)
                                                       tCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAGACAGACCCGCCGCCGACAAAcc                                       <  1:5342536/100‑1 (MQ=255)
                                                       tCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAAcc                                       <  1:646591/100‑1 (MQ=255)
                                                       tCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAAcc                                       <  2:2462409/100‑1 (MQ=255)
                                                        cATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCg                                      <  1:4238899/100‑1 (MQ=255)
                                                           cTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTgc                                   <  1:3490215/100‑1 (MQ=255)
                                                           cTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTgc                                   >  2:4435207/1‑100 (MQ=255)
                                                           cTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTgc                                   >  1:4137553/1‑100 (MQ=255)
                                                           cTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTgc                                   <  1:1014294/100‑1 (MQ=255)
                                                              gCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCa                                >  1:2523024/1‑100 (MQ=255)
                                                              gCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCa                                <  1:2294685/100‑1 (MQ=255)
                                                              gCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCa                                >  2:302648/1‑100 (MQ=255)
                                                              gCTCCACAGACCGCCCATCATGCGCATGTCGCGGGCACGGCTACGCTCATAAAGCTGACCACAAACAATAAACAGACCCGCCGCCCACCAACCGTTCGCa                                >  2:3650402/1‑100 (MQ=255)
                                                               cTCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCaa                               >  1:4030746/1‑100 (MQ=255)
                                                                tCCACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAat                              <  2:4854186/100‑1 (MQ=255)
                                                                 ccACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAatc                             >  1:3709815/1‑100 (MQ=255)
                                                                 ccACAGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAatc                             >  2:1938621/1‑100 (MQ=255)
                                                                  cacaGACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAatca                            <  1:1176996/100‑1 (MQ=255)
                                                                      gACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGCCCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTg                        >  2:2645526/1‑100 (MQ=255)
                                                                      gACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTg                        >  1:13384/1‑100 (MQ=255)
                                                                      gACCGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTg                        <  2:1396000/100‑1 (MQ=255)
                                                                         cGCCCATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGAt                     <  2:783285/100‑1 (MQ=255)
                                                                            ccATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTAc                  <  2:457785/100‑1 (MQ=255)
                                                                            ccATCATGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTAc                  <  2:1967369/100‑1 (MQ=255)
                                                                            ccATCATGCGCATGACGGGGGCATGGACACGTTCCTAAAGCGGACCACGAGGTATAATCAGCCCCGCCGCCGACAAGCCGTGCGCAATCATCTGGATTAc                  <  2:5257326/100‑1 (MQ=255)
                                                                              atcatGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcg                >  2:1467657/1‑100 (MQ=255)
                                                                              atcatGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcg                <  1:2702970/100‑1 (MQ=255)
                                                                               tcatGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgc               <  1:3704410/100‑1 (MQ=255)
                                                                               tcatGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgc               >  1:2019468/1‑100 (MQ=255)
                                                                               tcatGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgc               <  2:2772004/100‑1 (MQ=255)
                                                                                catGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgcg              >  1:4567093/1‑100 (MQ=255)
                                                                                catGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgcg              <  2:3275141/100‑1 (MQ=255)
                                                                                catGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgcg              <  1:941706/100‑1 (MQ=255)
                                                                                  tGCGCATGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCgcgcn            <  1:4431426/100‑2 (MQ=255)
                                                                                       aTGTCGCGGGTATGGATACGTTCAGAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGt       <  2:2369811/100‑1 (MQ=255)
                                                                                       aTGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGt       <  1:3261998/100‑1 (MQ=255)
                                                                                       aTGTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGt       <  1:4370076/100‑1 (MQ=255)
                                                                                         gTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGGCAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAg     >  2:4935228/1‑100 (MQ=255)
                                                                                         gTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAg     <  2:2850554/100‑1 (MQ=255)
                                                                                         gTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAg     >  1:4756801/1‑100 (MQ=255)
                                                                                         gTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAg     >  1:2963461/1‑100 (MQ=255)
                                                                                         gTCGCGGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAg     >  2:3732370/1‑100 (MQ=255)
                                                                                           cgcgGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGc   >  2:679158/1‑100 (MQ=255)
                                                                                           cgcgGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGATAGGc   >  2:679159/1‑100 (MQ=255)
                                                                                            gcgGGCATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGcc  <  2:2863084/100‑1 (MQ=255)
                                                                                                 |                                                                                              
CCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCC  >  NC_000913/2391843‑2392034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A