Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,612,663 T→G L255* (TTA→TGA)  hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,612,6630TG98.2% 790.2 / ‑0.4 229L255* (TTA→TGA) hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (122/103);  total (125/104)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

CCTGGCGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTTATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATAC  >  NC_000913/2612566‑2612759
                                                                                                 |                                                                                                
ccTGGCGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGtgat                                                                                                <  1:1243556/100‑1 (MQ=255)
  tGGCGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGtgatta                                                                                              >  2:4144752/1‑100 (MQ=255)
   ggCGAAAGTGTATTGCTGACACAAACGCTACAAACTCAACTACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTAc                                                                                             >  2:5362948/1‑100 (MQ=255)
   ggCGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTTATTAc                                                                                             >  2:3908757/1‑100 (MQ=255)
     cGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCTGTGATTActc                                                                                           >  2:2081136/1‑100 (MQ=255)
     cGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTActc                                                                                           >  2:3335549/1‑100 (MQ=255)
     cGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTActc                                                                                           >  1:203073/1‑100 (MQ=255)
     cGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTActc                                                                                           >  1:4998851/1‑100 (MQ=255)
      gAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTActct                                                                                          >  1:1274433/1‑100 (MQ=255)
       aaaGTGTATTGCTGACACAAACGCTACAAACCTGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCtt                                                                                         <  1:5099438/100‑1 (MQ=255)
         aGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTan                                                                                       <  1:4542679/100‑2 (MQ=255)
           tgtATTGCTGACACAAACTCTACAAACCCTCCATCCGACTTTGACGAACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACtt                                                                                     <  2:1394263/100‑1 (MQ=255)
           tgtATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGTAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACtt                                                                                     <  2:28828/100‑1 (MQ=255)
           tgtATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACtt                                                                                     >  1:3977813/1‑100 (MQ=255)
            gtATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTg                                                                                    <  2:1935842/100‑1 (MQ=255)
              aTTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCa                                                                                  <  2:3098847/100‑1 (MQ=255)
              aTTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCa                                                                                  >  1:4265606/1‑100 (MQ=255)
              aTTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCa                                                                                  >  1:2166857/1‑100 (MQ=255)
              aTTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCa                                                                                  >  1:2608002/1‑100 (MQ=255)
                  cTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTa                                                                              >  1:2460286/1‑100 (MQ=255)
                    gACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCTGTGATTACTCTTACTTGCATCTAAc                                                                            <  1:2103355/100‑1 (MQ=255)
                     acacAAACGCTACTAACCCGACAACCGACCTCGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACg                                                                           <  2:4306457/100‑1 (MQ=255)
                      cacaAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACgg                                                                          >  1:1653613/1‑100 (MQ=255)
                      cacaAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACgg                                                                          >  2:1667716/1‑100 (MQ=255)
                      cacaAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCGGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACgg                                                                          >  2:4683197/1‑100 (MQ=255)
                        caAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCt                                                                        >  1:3177725/1‑100 (MQ=255)
                        caAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCt                                                                        >  1:1826357/1‑100 (MQ=255)
                        caAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCt                                                                        >  1:3460257/1‑100 (MQ=255)
                         aaaCGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTg                                                                       >  1:466941/1‑100 (MQ=255)
                           aCGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGATTCTCTGGCAACGCGACCCGTGATTACTCATACTTGCATCTAACGGCTGCg                                                                     >  2:4263527/1‑100 (MQ=255)
                            cGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTGCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGa                                                                    >  2:5281254/1‑100 (MQ=255)
                             gCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATTTAACGGCTGCGaa                                                                   <  2:2821485/100‑1 (MQ=255)
                              cTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAAt                                                                  >  1:2837894/1‑100 (MQ=255)
                              cTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACCCTTACTTGCATCTAACGGCTGCGAAt                                                                  >  1:3273398/1‑100 (MQ=255)
                               tACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATc                                                                 <  1:5443889/100‑1 (MQ=255)
                               tACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGAACTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATc                                                                 >  2:3196615/1‑100 (MQ=255)
                                aCAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCt                                                                >  2:4093821/1‑100 (MQ=255)
                                aCAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCt                                                                >  1:1115535/1‑100 (MQ=255)
                                 cAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTg                                                               >  1:3484988/1‑100 (MQ=255)
                                  aaaCCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTgc                                                              <  1:5124863/100‑1 (MQ=255)
                                   aaCCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTgcg                                                             <  1:1028469/100‑1 (MQ=255)
                                   aaCCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTgcg                                                             >  2:3294477/1‑100 (MQ=255)
                                      ccGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTc                                                          >  1:2143384/1‑100 (MQ=255)
                                      ccGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTGCTCTGGCAACGCGACCCGGGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTc                                                          >  2:2622465/1‑100 (MQ=255)
                                       cGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTcc                                                         >  1:4142517/1‑100 (MQ=255)
                                           aaCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtat                                                     <  2:2388939/100‑1 (MQ=255)
                                           aaCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtat                                                     <  2:1872985/100‑1 (MQ=255)
                                           aaCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtat                                                     >  2:5469357/1‑100 (MQ=255)
                                           aaCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtat                                                     >  2:1310282/1‑100 (MQ=255)
                                           aaCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtat                                                     <  2:1189922/100‑1 (MQ=255)
                                            aCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtata                                                    >  1:4196051/1‑100 (MQ=255)
                                            aCCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTtata                                                    <  1:5520940/100‑1 (MQ=255)
                                              cGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATAcc                                                  <  2:2767796/100‑1 (MQ=255)
                                              cGACCTTGACGCACCGTGCAGATGAGCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCGTCTAACGGCTGCGAATCTGCGCTCCTTATAcc                                                  >  2:5501464/1‑100 (MQ=255)
                                                   ttGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTa                                             >  1:2940924/1‑100 (MQ=255)
                                                   ttGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTa                                             >  2:5455867/1‑100 (MQ=255)
                                                    tGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTAc                                            <  2:5352329/100‑1 (MQ=255)
                                                    tGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTAc                                            >  2:1692933/1‑100 (MQ=255)
                                                    tGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTAc                                            <  1:1127327/100‑1 (MQ=255)
                                                       cGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCtt                                         >  2:824128/1‑100 (MQ=255)
                                                       cGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCtt                                         >  2:5049539/1‑100 (MQ=255)
                                                         cACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTg                                       <  2:1421795/100‑1 (MQ=255)
                                                           ccGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGc                                     <  1:1712873/100‑1 (MQ=255)
                                                            cGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCa                                    >  2:3439199/1‑100 (MQ=255)
                                                            cGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCa                                    <  1:5038082/100‑1 (MQ=255)
                                                             gTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCaa                                   <  1:2319103/100‑1 (MQ=255)
                                                               gCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAAcc                                 <  1:352865/100‑1 (MQ=255)
                                                               gCAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCCGCGCTCCTTATACCGCTTACCTTTGGCAAcc                                 <  2:4990522/100‑1 (MQ=255)
                                                                cAGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCa                                >  1:433423/1‑100 (MQ=255)
                                                                 aGATGATCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCAt                               <  1:2804636/100‑1 (MQ=255)
                                                                  gatgatCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATa                              >  2:2284722/1‑100 (MQ=255)
                                                                     gatCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATacac                           <  1:615300/100‑1 (MQ=255)
                                                                      atCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACAcc                          <  1:3337686/100‑1 (MQ=255)
                                                                      atCTGTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACAcc                          <  1:5353615/100‑1 (MQ=255)
                                                                          gTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGt                      <  2:2518739/100‑1 (MQ=255)
                                                                          gTTTCTCTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGt                      >  1:3632735/1‑100 (MQ=255)
                                                                           tttCTCTGGCAACGCGACCCGAGATTACTCTTACTTGCATCTGACGGCTGCGAATCTGCGCCCCTTATACCGCTGACCTTTGGCAACTATACAccagctg                     >  2:1123394/1‑95 (MQ=255)
                                                                             tctcTGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCa                   >  1:2763682/1‑100 (MQ=255)
                                                                              ctctGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAt                  <  1:5397710/100‑1 (MQ=255)
                                                                               tctGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAtt                 <  1:2849649/100‑1 (MQ=255)
                                                                                ctGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttg                <  1:392740/100‑1 (MQ=255)
                                                                                ctGGCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttg                >  2:3832821/1‑100 (MQ=255)
                                                                                 tGGCAACGCGACCCGTGATTACTATTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttgt               >  1:1861821/1‑100 (MQ=255)
                                                                                  ggCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttgtt              >  2:515420/1‑100 (MQ=255)
                                                                                  ggCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttgtt              <  1:1399649/100‑1 (MQ=255)
                                                                                  ggCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttgtt              >  2:4171692/1‑100 (MQ=255)
                                                                                  ggCAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCAttgtt              >  1:3838891/1‑100 (MQ=255)
                                                                                    cAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGc            <  1:3587757/100‑1 (MQ=255)
                                                                                    cAACGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGc            <  1:871351/100‑1 (MQ=255)
                                                                                     aaCGCGACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCt           <  2:3576064/100‑1 (MQ=255)
                                                                                      aCGGGACCGTTTATTACTCTTACTTGTGTCTGAGGGCTGCTAATCTGTGCGCCTTATACCGTTTACCTTTGGCAACCATACACCGTGTGCATTGTTGCTg          <  2:1612073/100‑1 (MQ=255)
                                                                                       cgcgACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTgg         <  1:3604863/100‑1 (MQ=255)
                                                                                       cgcgACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTgg         >  2:4456688/1‑100 (MQ=255)
                                                                                       cgcgACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTgg         <  1:2368835/100‑1 (MQ=255)
                                                                                         cgACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGgcg       <  1:2824188/100‑1 (MQ=255)
                                                                                         cgACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGgcg       >  2:25731/1‑100 (MQ=255)
                                                                                         cgACCCGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGgcg       <  2:91365/100‑1 (MQ=255)
                                                                                            cccGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCAt    >  2:4017686/1‑100 (MQ=255)
                                                                                            cccGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCAt    >  1:3042191/1‑100 (MQ=255)
                                                                                             ccGTGATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTGCCTTTGGCAACCATACACCGGGCGCATTGTTGCTGGCGCATa   <  1:4746968/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CCTGGCGAAAGTGTATTGCTGACACAAACGCTACAAACCCGACAACCGACCTTGACGCACCGTGCAGATGATCTGTTTCTCTGGCAACGCGACCCGTTATTACTCTTACTTGCATCTAACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATAC  >  NC_000913/2612566‑2612759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A