Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,616,067 T→G intergenic (‑186/‑27) yfgO ← / → bepA putative UPF0118 family inner membrane permease/OM protein maintenance and assembly metalloprotease and chaperone, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,616,0670TG98.7% 520.2 / ‑2.8 153intergenic (‑186/‑27)yfgO/bepAputative UPF0118 family inner membrane permease/OM protein maintenance and assembly metalloprotease and chaperone, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (75/75);  total (76/77)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 154 total aligned reads displayed.

ATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCC  >  NC_000913/2615968‑2616162
                                                                                                   |                                                                                               
   cAGGAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAtc                                                                                              <  1:2942587/100‑1 (MQ=255)
   cAGGAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAtc                                                                                              <  1:1113570/100‑1 (MQ=255)
   cAGGAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAtc                                                                                              >  2:2898543/1‑100 (MQ=255)
   cAGGAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAtc                                                                                              >  1:1741592/1‑100 (MQ=255)
     ggAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAtctc                                                                                            >  2:3360318/1‑100 (MQ=255)
     ggAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAtctc                                                                                            >  1:2566463/1‑100 (MQ=255)
      gAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTcc                                                                                           >  2:3513375/1‑100 (MQ=255)
        aaCTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAg                                                                                         <  1:1333167/100‑1 (MQ=255)
        aaCTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACGCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAg                                                                                         <  1:282673/100‑1 (MQ=255)
         ncTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGa                                                                                        >  1:464644/2‑100 (MQ=255)
         aCTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACCCTCAAAGGCGGCGCGGTGGGCACGATATTTCACAGTATCGGTCAAAAGACCATCTCCAGa                                                                                        >  2:3081715/1‑100 (MQ=255)
           tGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGaaa                                                                                      >  1:801524/1‑100 (MQ=255)
           tGAAAAGTCATTTGAGCGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGaaa                                                                                      >  1:3111941/1‑100 (MQ=255)
            gAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAAt                                                                                     >  1:2356135/1‑100 (MQ=255)
               aaGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACa                                                                                  <  1:2137496/100‑1 (MQ=255)
                aGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAg                                                                                 <  1:265868/100‑1 (MQ=255)
                aGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAg                                                                                 <  2:2153819/100‑1 (MQ=255)
                 gTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAgg                                                                                <  1:3400815/100‑1 (MQ=255)
                   cATTTGAGTGTGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGAt                                                                              <  2:2806558/100‑1 (MQ=255)
                   cATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGAt                                                                              >  1:3309737/1‑100 (MQ=255)
                     tttGAGTGGGCTAATCTGCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATag                                                                            <  2:1446216/100‑1 (MQ=255)
                       tGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATagag                                                                          >  2:3745507/1‑100 (MQ=255)
                        gAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAgg                                                                         <  1:4035569/100‑1 (MQ=255)
                        gAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAgg                                                                         >  1:1617857/1‑100 (MQ=255)
                        gAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAgg                                                                         <  2:3351389/100‑1 (MQ=255)
                         aGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGt                                                                        >  2:3128427/1‑100 (MQ=255)
                            gggCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTAt                                                                     <  2:2143300/100‑1 (MQ=255)
                            gggCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTAt                                                                     <  2:668038/100‑1 (MQ=255)
                             ggCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATg                                                                    <  2:1837565/100‑1 (MQ=255)
                             ggCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATg                                                                    >  1:1798268/1‑100 (MQ=255)
                             ggCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATg                                                                    <  2:2798839/100‑1 (MQ=255)
                             ggCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATg                                                                    <  1:2272026/100‑1 (MQ=255)
                               cTAATCTTCGCCGTTGCACTCAAAGGCGGCGCGGTGTGAACGATATTTCACATTATCGTTCAGATGACGATCTCGAGAAATACAGGATAGAGGTTATGtt                                                                  >  2:1784309/1‑100 (MQ=255)
                               cTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGtt                                                                  >  2:167560/1‑100 (MQ=255)
                                tAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTc                                                                 >  2:1491418/1‑100 (MQ=255)
                                 aaTCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCa                                                                <  1:1073323/100‑1 (MQ=255)
                                 aaTCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCa                                                                <  2:2756933/100‑1 (MQ=255)
                                 aaTCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCa                                                                >  2:1069210/1‑100 (MQ=255)
                                 aaTCTTCGCCGCTACACTCAAAGGCGGCGCGGTGGGAACGCTATTTCACAGTATCGGTCAAATGACGATCTCTAGAAATACAGGATAGAGGTTATGTTCa                                                                <  1:3602230/100‑1 (MQ=255)
                                  aTCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAg                                                               >  1:3334066/1‑100 (MQ=255)
                                   tCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAgg                                                              <  2:3170028/100‑1 (MQ=255)
                                    cTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGc                                                             >  2:1708393/1‑100 (MQ=255)
                                    cTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGc                                                             >  1:977055/1‑100 (MQ=255)
                                    cTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGc                                                             <  1:1244976/100‑1 (MQ=255)
                                    cTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTACAGAAATACAGGATAGAGGTTATGTTCAGGc                                                             <  2:3154042/100‑1 (MQ=255)
                                         ccGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTg                                                        <  1:230546/100‑1 (MQ=255)
                                           gTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaa                                                      >  2:1293599/1‑100 (MQ=255)
                                            ttACATTAAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaa                                                     <  2:2694617/100‑1 (MQ=255)
                                            ttACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaan                                                     <  2:149313/100‑2 (MQ=255)
                                            ttACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaa                                                     >  2:2261559/1‑100 (MQ=255)
                                            ttACACTCAAAGGCGGCGCAGTGGGAACGAGATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaa                                                     <  2:3953899/100‑1 (MQ=255)
                                              acacTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaaaa                                                   <  1:538308/100‑1 (MQ=255)
                                              acacTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaaaa                                                   <  2:3846475/100‑1 (MQ=255)
                                                 cTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaaaaaaa                                                >  1:925070/1‑100 (MQ=255)
                                                 cTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGaaaaaaaa                                                >  1:2327323/1‑100 (MQ=255)
                                                  tCAAAGGCGGCGGGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGAACTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAAc                                               <  2:135421/100‑1 (MQ=255)
                                                   cAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAAcc                                              <  2:2436135/100‑1 (MQ=255)
                                                   cAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAAcc                                              >  1:3986543/1‑100 (MQ=255)
                                                   cAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAAcc                                              <  1:3554046/100‑1 (MQ=255)
                                                    aaaGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCt                                             <  1:3362018/100‑1 (MQ=255)
                                                      aGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAACAACAGGATAGAGGCGATGTTCGGGCAGTGGGAAAAAAACCTgg                                           >  2:2083378/1‑100 (MQ=255)
                                                      aGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTgg                                           <  2:3255715/100‑1 (MQ=255)
                                                      aGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTgg                                           <  1:3687921/100‑1 (MQ=255)
                                                       ggcggcGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGt                                          >  2:3659126/1‑100 (MQ=255)
                                                       ggcggcGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGt                                          >  2:3026728/1‑100 (MQ=255)
                                                        gcggcgCGGTGGGAACGATATTTCACAGTATCGTTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGtt                                         <  1:4037680/100‑1 (MQ=255)
                                                             gcgGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAAc                                    <  1:702383/100‑1 (MQ=255)
                                                              cgGTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAAcc                                   <  2:3911871/100‑1 (MQ=255)
                                                                gTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCt                                 >  1:3109445/1‑100 (MQ=255)
                                                                gTGGGAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCt                                 >  2:2716639/1‑100 (MQ=255)
                                                                 tGGGAACGATATTTCACAGTATCGGTCAACTGAAGCTCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTc                                >  1:2232721/1‑100 (MQ=255)
                                                                  gggAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCa                               >  2:2545186/1‑100 (MQ=255)
                                                                  gggAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCa                               >  2:752792/1‑100 (MQ=255)
                                                                   ggAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCAt                              >  1:2688905/1‑100 (MQ=255)
                                                                    gAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCAtt                             >  2:2231747/1‑100 (MQ=255)
                                                                    gAACGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCAtt                             >  1:1017842/1‑100 (MQ=255)
                                                                       cGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATtgct                          >  2:1913223/1‑100 (MQ=255)
                                                                       cGATATTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATtgct                          <  1:3759488/100‑1 (MQ=255)
                                                                        gATATTTCACAGTATCGGTCAAATGACGATATCCAGAAATACAGGATAGAGGCTATGCCCCGGCAGTTGAAAAACAACCCGCTTGCAAACCTCACcgctg                         >  2:1170509/1‑100 (MQ=255)
                                                                         atatTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATtgctgc                        <  1:1047919/100‑1 (MQ=255)
                                                                          tatTTCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTgctgct                       <  1:1330109/100‑1 (MQ=255)
                                                                            tttCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTAt                     >  1:1677100/1‑100 (MQ=255)
                                                                             ttCACAGTATCGGTCAAATGACGACCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATg                    <  1:2437390/100‑1 (MQ=255)
                                                                              tCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGa                   <  2:3062953/100‑1 (MQ=255)
                                                                              tCACAGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGa                   >  2:1565371/1‑100 (MQ=255)
                                                                                 caGTATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCa                <  2:848598/100‑1 (MQ=255)
                                                                                    tATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTg             >  2:643609/1‑100 (MQ=255)
                                                                                    tATCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTg             >  2:1432291/1‑100 (MQ=255)
                                                                                     aTCGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTgg            >  1:2483009/1‑100 (MQ=255)
                                                                                       cGGTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTc          <  2:909009/100‑1 (MQ=255)
                                                                                        ggTCAAATGACGATCTCCAGAAATACATGATAGACGTTATCTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCCTTGCTGCTATGACCATTGGTCa         <  2:3823513/100‑1 (MQ=255)
                                                                                        ggTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCa         >  2:2084445/1‑100 (MQ=255)
                                                                                        ggTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCa         >  1:1297432/1‑100 (MQ=255)
                                                                                         gTCAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAg        >  2:2605385/1‑100 (MQ=255)
                                                                                           cAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGt      >  1:2965653/1‑100 (MQ=255)
                                                                                           cAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGt      <  1:2287389/100‑1 (MQ=255)
                                                                                           cAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGt      >  1:4058249/1‑100 (MQ=255)
                                                                                           cAAATGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGt      <  2:1634615/100‑1 (MQ=255)
                                                                                            aaaTGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCAATGGTCatagc     <  1:292297/100‑5 (MQ=255)
                                                                                               tGACGATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGcc  <  2:2375325/100‑1 (MQ=255)
                                                                                                   |                                                                                               
ATTCAGGAAACTGAAAAGTCATTTGAGTGGGCTAATCTTCGCCGTTACACTCAAAGGCGGCGCGGTGGGAACGATATTTCACAGTATCGGTCAAATGACTATCTCCAGAAATACAGGATAGAGGTTATGTTCAGGCAGTTGAAAAAAAACCTGGTTGCAACCCTCATTGCTGCTATGACCATTGGTCAGGTAGCC  >  NC_000913/2615968‑2616162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A