Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,620,311 T→G N188H (AAC→CAC)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,620,3110TG98.6% 780.7 / ‑0.6 220N188H (AAC→CAC) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base G (106/111);  total (106/114)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 220 total aligned reads displayed.

TCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCACCAGAAC  >  NC_000913/2620215‑2620407
                                                                                                |                                                                                                
 ttaagaGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGt                                                                                              >  1:4954615/6‑100 (MQ=255)
 ctTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGt                                                                                              >  1:3835870/1‑100 (MQ=255)
  ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTc                                                                                             >  1:4728666/1‑100 (MQ=255)
  ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTc                                                                                             <  2:2654946/100‑1 (MQ=255)
    aaaGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTccc                                                                                           >  2:2056209/1‑100 (MQ=255)
    aaaGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTccc                                                                                           >  2:3639740/1‑100 (MQ=255)
    aaaGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTccc                                                                                           >  2:2402207/1‑100 (MQ=255)
     aaGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCt                                                                                          <  2:2604708/100‑1 (MQ=255)
      aGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTg                                                                                         <  2:3290531/100‑1 (MQ=255)
       gTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGa                                                                                        >  1:5217971/1‑100 (MQ=255)
       gTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGa                                                                                        <  1:5320329/100‑1 (MQ=255)
        tCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGAt                                                                                       <  2:186571/100‑1 (MQ=255)
        tCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGAt                                                                                       <  1:1681745/100‑1 (MQ=255)
         cGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATc                                                                                      <  1:3589603/100‑1 (MQ=255)
           gCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCaa                                                                                    <  1:487234/100‑1 (MQ=255)
           gCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCaa                                                                                    >  1:2139382/1‑100 (MQ=255)
            cTTTAATTATTTTTAGTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAAt                                                                                   <  1:3622681/100‑1 (MQ=255)
             tttAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATc                                                                                  <  2:2316388/100‑1 (MQ=255)
               tAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGa                                                                                <  1:1119420/100‑1 (MQ=255)
               tAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGa                                                                                >  1:1342852/1‑100 (MQ=255)
                    attTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCgg                                                                           <  1:1124047/100‑1 (MQ=255)
                     tttttATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGt                                                                          <  1:880654/100‑1 (MQ=255)
                       tttATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGtat                                                                        >  2:1775116/1‑100 (MQ=255)
                        ttATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGtata                                                                       <  2:4960286/100‑1 (MQ=255)
                        ttATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGtata                                                                       <  1:4764654/100‑1 (MQ=255)
                         tATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATAc                                                                      <  1:4601786/100‑1 (MQ=255)
                         tATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATAc                                                                      >  2:1034109/1‑100 (MQ=255)
                         tATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATAc                                                                      <  2:2912646/100‑1 (MQ=255)
                         tATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATAc                                                                      <  2:3542667/100‑1 (MQ=255)
                         tATTCTTTATTACTTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATAc                                                                      <  2:4395955/100‑1 (MQ=255)
                          aTTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACa                                                                     <  2:4047546/100‑1 (MQ=255)
                              tttATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTc                                                                 >  2:1382894/1‑100 (MQ=255)
                                tATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGa                                                               >  1:395076/1‑100 (MQ=255)
                                  tttCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACg                                                             >  2:2525655/1‑100 (MQ=255)
                                  tttCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACg                                                             <  1:2208224/100‑1 (MQ=255)
                                     cGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTcc                                                          >  2:2199548/1‑100 (MQ=255)
                                      gTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCg                                                         >  2:2873619/1‑100 (MQ=255)
                                      gTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCg                                                         <  1:3555358/100‑1 (MQ=255)
                                        aCCAAAGATTTTGTCCCCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCggg                                                       <  1:1960525/100‑1 (MQ=255)
                                        aCCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCggg                                                       <  2:391225/100‑1 (MQ=255)
                                          cAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTg                                                     <  1:1001498/100‑1 (MQ=255)
                                          cAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTg                                                     >  1:1071342/1‑100 (MQ=255)
                                           aaaGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTgc                                                    >  1:4142722/1‑100 (MQ=255)
                                            aattttttGTCACCGGCATCGCCGAGGCTTGGAATAATGTATCCGTGCTCGTGCAGGCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTgcg                                                   <  1:431862/96‑1 (MQ=255)
                                            aaGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTgcg                                                   <  2:4898123/100‑1 (MQ=255)
                                              gATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCt                                                 <  1:2706696/100‑1 (MQ=255)
                                              gATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCt                                                 >  2:5069890/1‑100 (MQ=255)
                                                 tttGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCtttt                                              <  2:4478957/100‑1 (MQ=255)
                                                 tttGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCtttt                                              <  2:3157244/100‑1 (MQ=255)
                                                  ttGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCttttt                                             <  1:1513044/100‑1 (MQ=255)
                                                  tggccACCGGCGTCGCCGGGGCCCGGCATGCTGTATCCGTGCTCGTGCAGTCCCTGTTCAGTTGATGCGGTATACAGTTCGACGTCCGAGTGCGCttttt                                             <  2:5013937/96‑1 (MQ=255)
                                                    gTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTcc                                           <  1:5395786/100‑1 (MQ=255)
                                                     tCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCca                                          <  1:696708/100‑1 (MQ=255)
                                                      cACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcag                                         >  1:2325680/1‑100 (MQ=255)
                                                      cACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcag                                         <  1:3113488/100‑1 (MQ=255)
                                                       aCCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagc                                        >  1:2851090/1‑100 (MQ=255)
                                                       aCCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagc                                        >  1:2064040/1‑100 (MQ=255)
                                                        ccGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcg                                       <  1:2244857/100‑1 (MQ=255)
                                                          ggCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgca                                     >  2:343860/1‑100 (MQ=255)
                                                          ggCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgca                                     >  2:1938934/1‑100 (MQ=255)
                                                           gCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgcag                                    >  2:1072046/1‑100 (MQ=255)
                                                           gCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgcag                                    <  1:500289/100‑1 (MQ=255)
                                                            cATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgcagc                                   >  2:433287/1‑100 (MQ=255)
                                                             aTCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgcagcg                                  <  2:1684994/100‑1 (MQ=255)
                                                             aTCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCcagcgcagcg                                  <  1:2450681/100‑1 (MQ=255)
                                                                gCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATa                               <  1:3157048/100‑1 (MQ=255)
                                                                gCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATa                               >  1:2799504/1‑100 (MQ=255)
                                                                gCCGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGCCGCGCTTTTTCCAGCGCACCGAtc                               >  1:4644611/1‑99 (MQ=255)
                                                                 ccGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATAc                              >  1:3766226/1‑100 (MQ=255)
                                                                 ccGAGGCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATAc                              >  1:3845249/1‑100 (MQ=255)
                                                                    aGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCtt                           <  1:3444043/100‑1 (MQ=255)
                                                                      gCCCGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCt                         >  1:3340402/1‑100 (MQ=255)
                                                                       cccGGAATACTGTATCCGTGCTCGTGCAGTCCCGGATCAATCGAGGCGGTATACAGTTCGACGTCCGGGTGCGCTTGTTCCAGCGCAGCGATACCTTCTg                        <  2:4712657/100‑1 (MQ=255)
                                                                       cccGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTg                        >  2:2546197/1‑100 (MQ=255)
                                                                       cccGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTg                        >  2:698775/1‑100 (MQ=255)
                                                                       cccGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTg                        >  1:1974104/1‑100 (MQ=255)
                                                                       cccGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTg                        <  1:1775916/100‑1 (MQ=255)
                                                                        ccGGAATAATGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTgg                       <  1:1400178/100‑1 (MQ=255)
                                                                            aataatGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCa                   <  1:2917015/100‑1 (MQ=255)
                                                                             ataatGTATCCGTGCTCGTGCGGTCCCTGATCAATCGATGCGGTATACAGTACGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAg                  >  2:4977872/1‑100 (MQ=255)
                                                                             ataatGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAg                  >  1:172326/1‑100 (MQ=255)
                                                                             ataatGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGGTCGACGTCCGGGTACGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAg                  >  2:5418452/1‑100 (MQ=255)
                                                                               aatGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACGTTCTGGCGCAGCt                >  2:3980460/1‑100 (MQ=255)
                                                                               aatGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCt                >  1:144728/1‑100 (MQ=255)
                                                                                atGTATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTa               <  2:2772675/100‑1 (MQ=255)
                                                                                   tATCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCa            >  1:3253860/1‑100 (MQ=255)
                                                                                    aTCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAg           >  2:937346/1‑100 (MQ=255)
                                                                                    aTCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAg           >  2:13247/1‑100 (MQ=255)
                                                                                    aTCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAg           <  1:5410636/100‑1 (MQ=255)
                                                                                    aTCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTCTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAg           >  2:3692155/1‑100 (MQ=255)
                                                                                     tCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGc          <  1:2901041/100‑1 (MQ=255)
                                                                                     tCCGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGc          >  1:1057245/1‑100 (MQ=255)
                                                                                      ccGTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCa         >  2:778911/1‑100 (MQ=255)
                                                                                      ccGTGCTCGTGCAGTCCCTGATCAATCAATGCGGTATACAGTTCGACGTCCGGGTGCGCATTTTCCAGCGCAGCGATACCTTCTGGCGAAGCTACCAGCa         >  1:4916718/1‑100 (MQ=255)
                                                                                        gTGCTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCAcc       <  2:5280927/100‑1 (MQ=255)
                                                                                           cTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCACCAGa    >  2:3316453/1‑100 (MQ=255)
                                                                                           cTCGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCACCAGa    >  1:3438948/1‑100 (MQ=255)
                                                                                             cGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCACCAGAAc  >  1:3237530/1‑100 (MQ=255)
                                                                                             cGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCACCAGAAc  >  1:3182047/1‑100 (MQ=255)
                                                                                             cGTGCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCCCAGCGATACCTTCTGGCGCAGCTCCCCGCACCAGAAc  >  2:4687563/1‑100 (MQ=255)
                                                                                                |                                                                                                
TCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCCAGCGCAGCGATACCTTCTGGCGCAGCTACCAGCACCAGAAC  >  NC_000913/2620215‑2620407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A