Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,186,531 T→G I394M (ATT→ATG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,5310TG99.1% 825.9 / ‑4.5 232I394M (ATT→ATG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (116/113);  total (118/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

GTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCA  >  NC_000913/4186434‑4186627
                                                                                                 |                                                                                                
gTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGaa                                                                                                <  2:5051184/100‑1 (MQ=255)
  cTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAg                                                                                              >  1:3336395/1‑100 (MQ=255)
   tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGc                                                                                             >  2:3679004/1‑100 (MQ=255)
        cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCga                                                                                        >  2:1967024/1‑100 (MQ=255)
         aGTGCGGTCTGCCGAAGAAACTGGATCTGGAGCTGTTCAAACCGTTCCTCTCCAGCCAGCTGGAACTGCGTGGTCTTGCTCCCACCATGAACGCTGCgac                                                                                       <  2:536158/100‑2 (MQ=255)
          gTGCGGTCTGCCGAAGCAAATGGCACTGGAGATGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCgacg                                                                                      >  1:1927958/1‑98 (MQ=255)
          gTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCgaag                                                                                      >  2:779577/1‑100 (MQ=255)
          gTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCgaag                                                                                      <  2:3639780/100‑1 (MQ=255)
          gTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCgaag                                                                                      >  2:4029164/1‑100 (MQ=255)
                tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATg                                                                                <  1:2306247/100‑1 (MQ=255)
                   gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGtt                                                                             >  1:5137923/1‑100 (MQ=255)
                   gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGtt                                                                             >  1:633649/1‑100 (MQ=255)
                   gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGtt                                                                             >  1:4369122/1‑100 (MQ=255)
                   gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGtt                                                                             <  2:563157/100‑1 (MQ=255)
                    tcGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTg                                                                            <  2:2305338/99‑1 (MQ=255)
                    ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTg                                                                            >  2:4085548/1‑100 (MQ=255)
                     cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTgc                                                                           >  2:2857698/1‑99 (MQ=255)
                     cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGa                                                                           >  2:3164038/1‑100 (MQ=255)
                     cGAAGAAAATGGCACTGGAGCGGTTCAAACCGTTCATCTACGACAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGa                                                                           >  1:5263094/1‑100 (MQ=255)
                        agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAgcg                                                                        <  1:4237815/100‑1 (MQ=255)
                        agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAgcg                                                                        <  1:1670142/100‑1 (MQ=255)
                         gaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAgcgc                                                                       >  1:2099358/1‑100 (MQ=255)
                                gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGaagaag                                                                <  2:2664146/100‑1 (MQ=255)
                                 cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                               <  2:5067192/100‑1 (MQ=255)
                                 cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                               >  1:3175744/1‑100 (MQ=255)
                                 cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                               >  2:317617/1‑100 (MQ=255)
                                 cACTGGAGCTGTTCAAACCGATCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGc                                                               <  2:17832/100‑1 (MQ=255)
                                  aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCt                                                              <  2:1701391/100‑1 (MQ=255)
                                  aCTGGAGCTGTTCAAACCGTTCAACTACGGCAAGCTGGAACTGCGTGGCCTTGCTACCACCATGAAAGCTGCGAACCAAATGGTTGACCGCGCAGCAGCt                                                              >  2:685193/1‑100 (MQ=255)
                                   cTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTg                                                             <  1:44445/100‑1 (MQ=255)
                                   cTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTg                                                             >  2:1654371/1‑100 (MQ=255)
                                    tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                            >  1:1583405/1‑100 (MQ=255)
                                     ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTc                                                           <  1:2684974/100‑1 (MQ=255)
                                     ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTc                                                           <  1:3914368/100‑1 (MQ=255)
                                        gCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTATCACCATGAAGGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGtt                                                        <  2:5358063/100‑1 (MQ=255)
                                        gCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGtt                                                        >  2:1790103/1‑100 (MQ=255)
                                        gCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGtt                                                        >  1:1230531/1‑100 (MQ=255)
                                          tGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                      <  2:4869129/100‑1 (MQ=255)
                                          tGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                      >  1:292188/1‑100 (MQ=255)
                                          tGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                      >  1:4302100/1‑100 (MQ=255)
                                          tGTTCAAACCGTCCATCTACGGCCAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGACGAAGCTGTCGTTTg                                                      <  2:383802/100‑1 (MQ=255)
                                           gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                     >  1:3702029/1‑100 (MQ=255)
                                           gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                     <  2:1108416/100‑1 (MQ=255)
                                            ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                    <  2:4262632/100‑1 (MQ=255)
                                             tCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAATCTGCGAAGAAAATGGTTGAGCGCGCAGACGCTGTCGTTTGGGa                                                   >  2:3285287/1‑100 (MQ=255)
                                             tCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                   <  1:2273284/100‑1 (MQ=255)
                                             tCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                   <  1:5118046/100‑1 (MQ=255)
                                                aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGatat                                                >  1:583618/1‑100 (MQ=255)
                                                 aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                               <  1:5335522/100‑1 (MQ=255)
                                                  ccGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATcc                                              >  1:3437486/1‑100 (MQ=255)
                                                     ttCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTgg                                           <  1:2330486/100‑1 (MQ=255)
                                                       cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGGAGCTGTCGTTTGGGATATCCTGGAc                                         <  2:2544216/100‑1 (MQ=255)
                                                       cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGAc                                         <  2:1610847/100‑1 (MQ=255)
                                                        aTCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAATAAGCTGTCGCTTGGGATACCCTGGACg                                        >  2:5256805/1‑100 (MQ=255)
                                                            aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                    <  2:3115570/100‑1 (MQ=255)
                                                            aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                    <  2:2941767/100‑1 (MQ=255)
                                                            aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                    <  1:3309830/100‑1 (MQ=255)
                                                            aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                    >  2:4423775/1‑100 (MQ=255)
                                                            aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                    <  1:2708543/100‑1 (MQ=255)
                                                            aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                    <  2:4119210/100‑1 (MQ=255)
                                                             cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGtt                                   >  1:4587968/1‑100 (MQ=255)
                                                              gtcAAGCTGGAACGGCGGGGTCTTTCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTa                                  >  2:3029615/3‑100 (MQ=255)
                                                               gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTAt                                 >  1:937505/1‑100 (MQ=255)
                                                                cAAGCTGGAACTGCGTGGTCTTGCTACCACCATGACAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCCGTCGTTTGGGATATCGTTGACGAAgccatc                                >  2:3233523/1‑95 (MQ=255)
                                                                  aGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcg                              <  2:4685928/100‑1 (MQ=255)
                                                                  aGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAACCTGTCGTTTGGGATATCCTGGACGAAGCTATCcg                              >  2:739885/1‑100 (MQ=255)
                                                                   gCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                             >  2:4573119/1‑100 (MQ=255)
                                                                     tGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                           <  1:3159081/100‑1 (MQ=255)
                                                                      ggAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGac                          <  1:314062/100‑2 (MQ=255)
                                                                       gAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGCTATCCGCGacc                         >  2:4281749/1‑98 (MQ=255)
                                                                        aaCTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAcca                        <  2:5480116/100‑1 (MQ=255)
                                                                        aaCTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAcca                        >  1:5442629/1‑100 (MQ=255)
                                                                          cTGCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaccacc                      <  1:3573742/100‑1 (MQ=255)
                                                                            gCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCg                    <  1:4681200/100‑1 (MQ=255)
                                                                            gCGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGCTGAGCGCGAAGAAGCTGTCCCTTGGGATATCCTCGACGAAGTTATCCGCGACCACCCg                    >  2:3401706/1‑100 (MQ=255)
                                                                             cGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCgg                   <  1:521080/100‑1 (MQ=255)
                                                                             cGTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCgg                   <  2:2166917/100‑1 (MQ=255)
                                                                              gTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGt                  <  1:1499842/100‑1 (MQ=255)
                                                                              gTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGt                  <  2:1439033/100‑1 (MQ=255)
                                                                              gTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGt                  >  2:2488388/1‑100 (MQ=255)
                                                                              gTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGt                  >  1:4499299/1‑100 (MQ=255)
                                                                              gTGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGt                  <  1:3852384/100‑1 (MQ=255)
                                                                               tGGTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGCTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTa                 >  1:2495008/1‑100 (MQ=255)
                                                                                ggTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTAc                <  2:3736825/100‑1 (MQ=255)
                                                                                ggTCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTAc                <  1:768392/100‑1 (MQ=255)
                                                                                  tCTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTActg              <  1:1479532/100‑1 (MQ=255)
                                                                                   cTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTActgc             >  2:5019549/1‑100 (MQ=255)
                                                                                   cTTGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTActgc             <  2:3352985/100‑1 (MQ=255)
                                                                                     tGCTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTActgctg           <  1:5516357/100‑1 (MQ=255)
                                                                                       cTACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGaa         >  1:2997516/1‑100 (MQ=255)
                                                                                        tACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAAc        >  1:4929293/1‑100 (MQ=255)
                                                                                        tACCACCATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAAc        <  2:1266132/100‑1 (MQ=255)
                                                                                         accaccATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAAcc       >  1:3619927/1‑100 (MQ=255)
                                                                                         accaccATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAAcc       >  1:1659237/1‑100 (MQ=255)
                                                                                         accaccATGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAAcc       >  1:4642229/1‑100 (MQ=255)
                                                                                          ccaccaTGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAACCg      <  1:798144/100‑1 (MQ=255)
                                                                                          ccaccaTGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAACCg      >  1:1019794/1‑100 (MQ=255)
                                                                                            accaTGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAACCGTg    >  1:4108126/1‑100 (MQ=255)
                                                                                            accaTGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAACCGTg    <  2:581650/100‑1 (MQ=255)
                                                                                              caTGAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGACCACCCGGTACTGCTGAACCGTGCa  >  1:2153614/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCA  >  NC_000913/4186434‑4186627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A