Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F236 I1 R1 155 93.2 2393300 97.8% 2340647 180.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,375,801 T→G E54D (GAA→GAC)  menC ← O‑succinylbenzoyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,8010TG100.0% 103.6 / NA 31E54D (GAA→GAC) menCO‑succinylbenzoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/14);  total (17/14)

CGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGG  >  NC_000913/2375573‑2376007
                                                                                                                                                                                                                                     |                                                                                                                                                                                                              
cGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCctgactga                                                                                                                                                                                                      >  2:511071/1‑239 (MQ=255)
    ccTTTCTCGCACTGCATCTCTG‑GATTTTGACGGATCAGCTCGACGGGATCGCCATTCCCCAGCGGTGCCGCACGGGCTTTGGCTGCTTGCGGCAACGTATCTGACAGTTCTGCCAATGCCCAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTGATTTACCCAGGCAAGCAGCACGCGTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGcc                                                                                                                                                                                                  <  2:670393/238‑1 (MQ=255)
       ttCTCGCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCgg                                                                                                                                                                                               <  1:879900/239‑1 (MQ=255)
         ctcCCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCa                                                                                                                                                                                             <  2:1045353/239‑1 (MQ=255)
          tcGCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCa                                                                                                                                                                                             >  2:828043/1‑238 (MQ=255)
          tcGCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCa                                                                                                                                                                                             <  1:828043/238‑1 (MQ=255)
                  cATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACATCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACATTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTAGGgaca                                                                                                                                                                                    >  1:815100/1‑237 (MQ=255)
                            cgTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCTCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCa                                                                                                                                                                          <  2:815100/238‑1 (MQ=255)
                                                     tCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCa                                                                                                                                                   <  2:267261/238‑1 (MQ=255)
                                                                    ggTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACAt                                                                                                                                          <  2:456557/232‑1 (MQ=255)
                                                                      tGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCa                                                                                                                                                                                             <  1:252146/179‑1 (MQ=255)
                                                                      tGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCa                                                                                                                                                                                             >  2:252146/1‑179 (MQ=255)
                                                                      tGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGcc                                                                                                                                                   >  1:547991/1‑220 (MQ=255)
                                                                      tGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCa                                                                                                                                                   <  2:547991/221‑1 (MQ=255)
                                                                      tGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCCCTCGCCTTCCAGCAGGCAAACATACAg                                                                                                                                      >  1:195596/1‑234 (MQ=255)
                                                                          gCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTcgc                                                                                                                              <  1:511071/238‑1 (MQ=255)
                                                                           cACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCCGGCAAAAATAAAGCCCGTAGCgg                                                                                                                            >  1:323466/1‑239 (MQ=255)
                                                                                          ctTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTggg                                                                                                                                                                                        >  1:815565/1‑164 (MQ=255)
                                                                                          ctTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTggg                                                                                                                                                                                        <  2:815565/164‑1 (MQ=255)
                                                                                          ctTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGAt                                                                                                                                                                                   >  2:255557/1‑169 (MQ=255)
                                                                                          ctTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGAt                                                                                                                                                                                   <  1:255557/169‑1 (MQ=255)
                                                                                                           gTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGGTTTTAACCGACTGCCGCCCAGAACCAccc                                                                                             >  1:44571/1‑238 (MQ=255)
                                                                                                                                cTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCa                                                                       >  1:760143/1‑239 (MQ=255)
                                                                                                                                 ttACGCCAAAGGCCACTGAAGGCATCTGCGGTAGCTCGCACTCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGTTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGc                                                                         <  1:842047/236‑1 (MQ=255)
                                                                                                                                                      gCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTTTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCCGTATACCTGCCCTCTACGc                                                 >  2:1055287/1‑239 (MQ=255)
                                                                                                                                                                                  cagccagTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGaa                     >  1:848088/1‑239 (MQ=255)
                                                                                                                                                                                                    ggCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGCCGCGGGGTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCCACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACggt       >  2:761098/1‑233 (MQ=255)
                                                                                                                                                                                                     gCAAGCAGCACACTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGGCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACCCATTACGGACTCCGTTTGAATTTGCTGAAGTCAGGCCGACGTTTCTgg  >  1:121335/1‑239 (MQ=255)
                                                                                                                                                                                                             cacaCTTTGCGCCTCTTCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGCATACCTGCGCGCTACGCATTACGGAtt                                       >  1:401110/1‑194 (MQ=255)
                                                                                                                                                                                                                  tttGCCCCTCTTCCCAGGTGTCCTGCCGGAACCCCGGCAGGGGGGAGACCCCCCCCCCCCCTTCGCGCCCGCCTCCACGCAGGAAAACATCCAGCCCGTCGGGGTTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGcc                                                                        <  2:456112/156‑1 (MQ=255)
                                                                                                                                                                                                                            ttCCCAGGTGTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCCCCCTTCCCCCTCCCCTTCACGCAGGCAAACATACAGCCCGCCCCGGGTTTTTAACCGCCTGTCCCGCAGAACCACCCCCCCCTCCATGGGGATCTGCCCGCGGTATAc                                                              >  1:456112/1‑156 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                              
CGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGA‑GGATCAGATCGTCCGGATCGCCATTACACAGCGGTGCCGCACGGTAGTTGGCTGCTTGCGGCAACGTATCTGTCAGTTCTGCCAATGCACAGCTTACGCCAAAGGCCACGGAAGGCATCTGCGGTAGCTCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCCCCACCCTTCGCGCTCGCCTTCACGCAGGCAAACATACAGCCCGTCGCGGGTTTTTAACCGCCTGTCGCGCAGAACCACCCCCGCGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATTACGGATTCCGTTTGAATTTGCTGAAGTCAGGCTGACGTTTCTGG  >  NC_000913/2375573‑2376007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A