breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 6,809 T→G K384T (AAA→ACA)  yaaJ ← putative transporter
RA 47,341 T→G I32M (ATT→ATG kefF → potassium‑efflux system ancillary protein for KefC, glutathione‑regulated; quinone oxidoreductase, FMN‑dependent
RA 58,850 T→G pseudogene (377/579 nt) yabP → pseudogene, pentapeptide repeats‑containing
RA 73,145 T→G K459N (AAA→AAC thiP ← thiamine/thiamine pyrophosphate ABC transporter permease
RA 84,958 T→G I197M (ATT→ATG leuO → global transcription factor
RA 95,418 T→G S257A (TCG→GCG)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
RA 104,147 T→G S56A (TCC→GCC)  ftsA → ATP‑binding cell division FtsK recruitment protein
RA 109,887 A→C I537L (ATT→CTT)  secA → preprotein translocase subunit, ATPase
JC JC 111,051 IS1 (–) +9 bp coding (8‑16/390 nt) mutT → dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
RA 113,356 A→C intergenic (‑137/‑88) coaE ← / → guaC dephospho‑CoA kinase/GMP reductase
RA 138,704 A→C intergenic (+71/+131) cueO → / ← gcd multicopper oxidase (laccase)/glucose dehydrogenase
RA 151,435 A→C L55L (CTT→CTG yadK ← putative fimbrial‑like adhesin protein
RA 162,349 A→C Y82S (TAC→TCC)  hrpB → putative ATP‑dependent helicase
RA 180,109 T→G L291L (CTT→CTG dgt → deoxyguanosine triphosphate triphosphohydrolase
RA 242,498 A→C L269R (CTG→CGG)  fadE ← acyl coenzyme A dehydrogenase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 295,811 T→G E429A (GAA→GCA)  intF ← CP4‑6 prophage; putative phage integrase
RA 343,447 T→G H188Q (CAT→CAG yahK → broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
RA 515,620 T→G M52L (ATG→CTG)  qmcA ← PHB domain membrane‑anchored putative protease
RA 556,667 A→C V69V (GTT→GTG ybcJ ← ribosome‑associated protein; putative RNA‑binding protein
RA 605,633 A→C V85G (GTG→GGG)  ybdF ← DUF419 family protein
RA 607,856 T→G L7L (CTT→CTG hokE → toxic polypeptide, small
RA 619,006 A→C G251G (GGA→GGC fepE → regulator of length of O‑antigen component of lipopolysaccharide chains
RA 624,319 A→C D64E (GAT→GAG fepB ← ferrienterobactin ABC transporter periplasmic binding protein
RA 682,878 A→C D172E (GAT→GAG hscC ← Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
RA 691,280 T→G I169L (ATT→CTT)  ybeX ← putative ion transport
RA 699,371 T→G intergenic (‑194/+203) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 743,458 A→C Y211S (TAT→TCT)  ybgI → NIF3 family metal‑binding protein
RA 780,168 T→G Y191D (TAC→GAC)  ybgF → periplasmic TolA‑binding protein
RA 825,545 T→G V188V (GTA→GTC ybhR ← putative ABC transporter permease
RA 906,631 T→G E41D (GAA→GAC ybjS ← putative NAD(P)H‑dependent oxidoreductase
RA 930,957 A→C N2K (AAT→AAG cydD ← glutathione/cysteine ABC transporter export permease/ATPase
RA 946,920 T→G D350E (GAT→GAG ycaD → putative MFS transporter, inner membrane protein
RA 960,657 C→T L132L (CTG→TTG)  ycaL → putative peptidase‑related chaperone
RA 1,009,417 A→C D525A (GAT→GCT)  rlmL → 23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM‑dependent
RA 1,078,882 T→G M1M (ATG→CTG) † putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,086,572 T→G K425N (AAA→AAC pgaC ← biofilm PGA synthase PgaCD, catalytic subunit; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA 1,139,916 T→G D513E (GAT→GAG flgK → flagellar hook‑filament junction protein 1
RA 1,176,289 T→G L288R (CTG→CGG)  lolC → lipoprotein‑releasing system transmembrane protein
RA 1,183,048 T→G L191F (TTA→TTC potC ← spermidine/putrescine ABC transporter permease
RA 1,192,441 A→C V64G (GTC→GGC)  hflD ← putative lysogenization regulator
RA 1,197,360 A→C I58S (ATC→AGC)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,297,609 A→C D171E (GAT→GAG adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,311,079 T→G intergenic (‑214/‑10) yciI ← / → tonB putative DGPF domain‑containing enzyme/membrane spanning protein in TonB‑ExbB‑ExbD transport complex
RA 1,391,771 A→C F28C (TTT→TGT)  ycjY ← S9 homolog non‑peptidase family protein
RA 1,402,453 T→G Q268P (CAG→CCG)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit
RA 1,417,487 T→G intergenic (‑101/+1) recE ← / ← racC Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease/Rac prophage; uncharacterized protein
RA 1,588,280 T→G K6T (AAG→ACG)  ydeR ← putative fimbrial‑like adhesin protein
RA 1,632,251 A→C pseudogene (35/1284 nt) ydfJ ← pseudogene, MFS transporter family; interrupted by Qin prophage;Phage or Prophage Related; putative transport protein
RA 1,671,310 A→C intergenic (+358/‑66) ynfM → / → asr putative arabinose efflux transporter/acid shock‑inducible periplasmic protein
RA 1,688,772 A→C K66T (AAA→ACA)  manA → mannose‑6‑phosphate isomerase
RA 1,714,643 T→G I89M (ATT→ATG gstA → glutathionine S‑transferase
RA 1,756,688 A→C I331L (ATC→CTC)  pykF → pyruvate kinase I
RA 1,768,120 A→C I189S (ATT→AGT)  ydiJ ← putative FAD‑linked oxidoreductase
RA 1,815,451 T→G F529V (TTT→GTT)  katE → catalase HPII, heme d‑containing
RA 1,822,043 T→G I72L (ATC→CTC)  osmE ← osmotically‑inducible lipoprotein
RA 1,832,553 T→G F42L (TTT→TTG xthA → exonuclease III
RA 1,905,833 T→G L49R (CTG→CGG)  mntP → putative Mn(2+) efflux pump, mntR‑regulated
RA 1,907,569 T→G E8A (GAA→GCA)  yobF ← DUF2527 family heat‑induced protein
RA 1,920,539 T→G L106W (TTG→TGG)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,937,568 A→C intergenic (+47/‑81) yebK → / → pykA putative DNA‑binding transcriptional regulator/pyruvate kinase II
RA 1,973,627 T→G K566N (AAA→AAC cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,008,526 A→C I84L (ATC→CTC)  yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,049,948 T→G S1671A (TCT→GCT)  yeeJ → putative adhesin
RA 2,168,327 A→C intergenic (‑21/‑385) yegR ← / → yegS uncharacterized protein/phosphatidylglycerol kinase, metal‑dependent
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,226,626 A→C H215Q (CAT→CAG yohF ← putative oxidoreductase
RA 2,278,251 A→C intergenic (‑14/+319) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,290,390 A→C intergenic (‑210/‑110) yejO ← / → narP pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system/response regulator in two‑component regulatory system with NarQ
RA 2,298,335 T→G V128V (GTA→GTC napB ← nitrate reductase, small, cytochrome C550 subunit, periplasmic
RA 2,311,986 A→C L255R (CTG→CGG)  ompC ← outer membrane porin protein C
RA 2,318,639 A→C F413C (TTT→TGT)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,323,004 T→G I380S (ATC→AGC)  atoC → fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein
RA 2,375,801 T→G E54D (GAA→GAC menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,387,305 G→A P124P (CCG→CCA yfbM → DUF1877 family protein
RA 2,404,261 A→C L118R (CTG→CGG)  nuoB ← NADH:ubiquinone oxidoreductase, chain B
RA 2,408,074 A→C I172L (ATC→CTC)  alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
RA 2,414,947 T→G A67A (GCT→GCG pta → phosphate acetyltransferase
RA 2,419,184 A→C intergenic (‑8/+50) yfcD ← / ← yfcE putative NUDIX hydrolase/phosphodiesterase activity on bis‑pNPP
RA 2,430,317 A→C F149L (TTT→TTG cvpA ← colicin V production protein
RA 2,492,088 T→G L389F (TTA→TTC frc ← formyl‑CoA transferase, NAD(P)‑binding
RA 2,523,162 A→C L275* (TTA→TGA)  xapB ← xanthosine transporter
RA 2,656,000 T→G E120A (GAA→GCA)  pepB ← aminopeptidase B
RA 2,667,681 T→G S56R (AGT→CGT)  hcaT ← putative 3‑phenylpropionic transporter
RA 2,667,825 A→C W8G (TGG→GGG)  hcaT ← putative 3‑phenylpropionic transporter
RA 2,706,202 T→G E308A (GAA→GCA)  lepA ← back‑translocating elongation factor EF4, GTPase
RA 2,737,414 A→C K87N (AAA→AAC raiA → cold shock protein associated with 30S ribosomal subunit
RA 2,778,463 T→G K1422Q (AAA→CAA)  ypjA ← adhesin‑like autotransporter
RA 2,874,651 T→G N257H (AAT→CAT)  cysN ← sulfate adenylyltransferase, subunit 1
RA 2,876,976 T→G N132K (AAT→AAG iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 2,899,375 A→C I15S (ATT→AGT)  ygcU ← putative FAD‑linked oxidoreductase
RA 2,904,306 T→G intergenic (+932/+441) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,982,326 T→G intergenic (‑144/+171) araE ← / ← kduD arabinose transporter/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase; KDG oxidoreductase; 20‑ketosteroid reductase
RA 3,009,624 A→C K287N (AAA→AAC ygeY → putative peptidase
RA 3,025,296 T→G S316A (TCA→GCA)  xanQ → xanthine permease
RA 3,076,684 A→C F258C (TTC→TGC)  yggP ← putative Zn‑binding dehydrogenase
RA 3,088,259 A→C intergenic (+399/‑25) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter
RA 3,113,280 T→G V66V (GTA→GTC yghG ← secretin (GspDbeta) OM localization lipoprotein pilotin
RA 3,135,804 T→G I190L (ATT→CTT)  pitB ← phosphate transporter
RA 3,157,644 A→C intergenic (+194/‑6) dkgA → / → yqhG 2,5‑diketo‑D‑gluconate reductase A/DUF3828 family putative periplasmic protein
RA 3,173,660 A→C L579L (CTT→CTG parE ← DNA topoisomerase IV, subunit B
RA 3,175,124 A→C L91L (CTT→CTG parE ← DNA topoisomerase IV, subunit B
RA 3,193,396 A→C E178A (GAA→GCA)  yqiK → PHB family membrane protein, function unknown
RA 3,198,644 A→C F333C (TTC→TGC)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,218,426 A→C H217Q (CAT→CAG aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
RA 3,328,817 T→G intergenic (‑102/‑146) greA ← / → dacB transcript cleavage factor/D‑alanyl‑D‑alanine carboxypeptidase
RA 3,334,302 A→C D198A (GAT→GCT)  ispB → octaprenyl diphosphate synthase
RA 3,336,051 A→C D148E (GAT→GAG murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,341,805 A→C K180N (AAA→AAC kdsD → D‑arabinose 5‑phosphate isomerase
RA 3,349,202 T→G N203T (AAT→ACT)  mtgA ← biosynthetic peptidoglycan transglycosylase
RA 3,399,779 A→C L67R (CTG→CGG)  mreC ← cell wall structural complex MreBCD transmembrane component MreC
RA 3,468,138 T→G G572G (GGA→GGC chiA ← periplasmic endochitinase
RA 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7
RA 3,492,131 A→C F56C (TTT→TGT)  ppiA ← peptidyl‑prolyl cis‑trans isomerase A (rotamase A)
RA 3,499,258 A→C intergenic (+57/‑190) cysG → / → yhfL fused siroheme synthase 1,3‑dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase/small lipoprotein
RA 3,535,528 T→G Q114P (CAG→CCG)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,571,007 T→G K105Q (AAA→CAA)  glgX ← glycogen debranching enzyme
RA 3,616,561 T→G V127G (GTT→GGT)  nikC → nickel ABC transporter permease
RA 3,618,985 A→C D133A (GAT→GCT)  nikR → transcriptional repressor, Ni‑binding
RA 3,629,388 T→G K51Q (AAG→CAG)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,632,997 T→G pseudogene (269/414 nt) yhiL ← pseudogene, DUF4049 family protein
RA 3,640,752 T→G intergenic (+207/‑110) uspA → / → dtpB universal stress global response regulator/dipeptide and tripeptide permease B
RA 3,708,873 A→C C535G (TGT→GGT)  eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,727,303 A→C L20R (CTC→CGC)  yiaA ← YiaAB family inner membrane protein, tandem domains
RA 3,728,925 T→G V149V (GTA→GTC xylB ← xylulokinase
RA 3,749,326 A→C D32A (GAT→GCT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,752,058 A→C I23L (ATC→CTC)  yiaU → putative DNA‑binding transcriptional regulator
RA 3,772,303 A→C K8T (AAA→ACA)  mtlA → mannitol‑specific PTS enzyme: IIA, IIB and IIC components
RA 3,800,808 T→G D53A (GAT→GCT)  waaY ← lipopolysaccharide core biosynthesis protein
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 3,823,774 A→C N459H (AAC→CAC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,829,992 T→G Y350D (TAT→GAT)  xanP → xanthine permease
RA 3,923,819 A→C S605S (TCT→TCG mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,964,727 A→C F302V (TTT→GTT)  rhlB ← ATP‑dependent RNA helicase
RA 4,003,071 T→G A41A (GCA→GCC yigG ← PRK11371 family inner membrane protein
RA 4,046,716 A→C intergenic (‑114/‑250) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
RA 4,061,235 A→C Y24S (TAT→TCT)  yihM → putative sugar phosphate isomerase
RA 4,118,936 T→G N132T (AAT→ACT)  rraA ← ribonuclease E (RNase E) inhibitor protein
RA 4,158,027 T→G V393G (GTG→GGG)  argH → argininosuccinate lyase
RA 4,186,603 A→C E418D (GAA→GAC rpoC → RNA polymerase, beta prime subunit
RA 4,193,152 A→C L142R (CTC→CGC)  thiF ← adenylyltransferase, modifies ThiS C‑terminus
RA 4,249,540 T→G intergenic (+229/‑14) lamB → / → malM maltose outer membrane porin (maltoporin)/maltose regulon periplasmic protein
RA 4,289,061 A→C D433A (GAT→GCT)  nrfA → nitrite reductase, formate‑dependent, cytochrome
RA 4,291,339 T→G I288M (ATT→ATG nrfD → formate‑dependent nitrite reductase, membrane subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,514,053 T→G T86P (ACG→CCG)  fecB ← ferric citrate ABC transporter periplasmic binding protein
RA 4,517,146 T→G N191T (AAT→ACT)  fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,552,330 T→G V232G (GTT→GGT)  uxuA → mannonate hydrolase
RA 4,571,499 A→C L26V (TTG→GTG)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase
RA 4,572,080 T→G intergenic (+165/‑334) yjiS → / → yjiT DUF1127 family protein/pseudogene

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423877–3424233 3424463–3424242 10–587 18 [17] [16] 18 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =17 (0.190)82 (1.000) 62/328 0.2 83.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.210)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780517 (0.190)39 (0.480) 32/328 1.1 70.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.210)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related