Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 428,252 T→G I202M (ATT→ATG)  secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913428,2520TG96.7% 504.7 / 2.9 153I202M (ATT→ATG) secDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (74/74);  total (75/78)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 156 total aligned reads displayed.

AAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCT  >  NC_000913/428155‑428347
                                                                                                 |                                                                                               
   aaaaCAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATcagcag                                                                                            >  1:3859041/1‑100 (MQ=255)
    aaaCAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCAATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGCTGagcagc                                                                                           >  2:2704672/1‑100 (MQ=255)
       caacTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAg                                                                                        >  1:3737584/1‑100 (MQ=255)
        aacTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAgg                                                                                       <  1:1982425/100‑1 (MQ=255)
          cTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGgc                                                                                     <  2:3514119/100‑1 (MQ=255)
            aCGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGgcag                                                                                   <  1:1436674/100‑1 (MQ=255)
             cGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGgcagc                                                                                  <  1:988713/100‑1 (MQ=255)
               gCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCaa                                                                                <  1:3407132/100‑1 (MQ=255)
               gCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCaa                                                                                <  1:1230303/100‑1 (MQ=255)
               gCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCaa                                                                                >  1:3725815/1‑100 (MQ=255)
                ccTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAAc                                                                               >  2:2272896/1‑100 (MQ=255)
                ccTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAAc                                                                               >  1:2184727/1‑100 (MQ=255)
                ccTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAAc                                                                               >  2:958510/1‑100 (MQ=255)
                 cTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAAcc                                                                              >  1:3667763/1‑100 (MQ=255)
                  tGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCa                                                                             >  2:956497/1‑100 (MQ=255)
                  tGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCa                                                                             <  1:940380/100‑1 (MQ=255)
                    aGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGc                                                                           <  2:857889/100‑1 (MQ=255)
                    aGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGc                                                                           >  1:3602555/1‑100 (MQ=255)
                    aGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGc                                                                           <  2:4001378/100‑1 (MQ=255)
                     gCATCACTTTCCGCGATGGTAAAGCTCGTGATGAAGCCATTGCGGATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCt                                                                          <  2:2514549/100‑1 (MQ=255)
                        tCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCtgcg                                                                       <  1:2669021/100‑1 (MQ=255)
                        tCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCtgcg                                                                       <  2:2429594/100‑1 (MQ=255)
                        tCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCtgcg                                                                       >  1:438835/1‑100 (MQ=255)
                           cTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc                                                                    >  2:316794/1‑100 (MQ=255)
                           cTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCCGCCAGGGCAGCAACCAGCtgcgtgc                                                                    >  2:2212273/1‑100 (MQ=255)
                           cTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCtgcgtgc                                                                    <  1:1241878/100‑1 (MQ=255)
                           cTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCCGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCtgcgtgc                                                                    >  2:2833389/1‑100 (MQ=255)
                            tttCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCtgcgtgcg                                                                   >  1:240122/1‑100 (MQ=255)
                             ttCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCgg                                                                  <  2:2054814/100‑1 (MQ=255)
                               ccGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTa                                                                >  1:1445736/1‑100 (MQ=255)
                                cgcgATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTaa                                                               >  2:3695986/1‑100 (MQ=255)
                                cgcgATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTaa                                                               >  1:1042923/1‑100 (MQ=255)
                                cgcgATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTaa                                                               >  1:2531737/1‑100 (MQ=255)
                                 gcgATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAAt                                                              <  2:3491596/100‑1 (MQ=255)
                                  agctGCTAAAGCTCGTAAAGAAGAAATAGCGTATCTGAGCAAGACCCAACCGGACCTGGAAATGCGCCGCCCGCGCAGCCAAAAGCTGCGTGCGGTaaag                                                             <  2:1387276/97‑3 (MQ=255)
                                   gATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGa                                                            >  1:749881/1‑100 (MQ=255)
                                     tGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCGGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGc                                                          <  2:520723/100‑1 (MQ=255)
                                     tGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGc                                                          >  2:1771584/1‑100 (MQ=255)
                                      gCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCg                                                         >  1:332749/1‑100 (MQ=255)
                                         aaaGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGAACCCCGTGCAGCAACCAGCCGCGTGCGGTAATGAGCGATg                                                      >  2:1604086/1‑100 (MQ=255)
                                         aaaGCTCGTGATGAAGCCATTGAGTATCTGAGCAAGCACCATCCGGAACTTGTGATGAGCAGCCAGGGGAACCACCAGCTACATGCGGTAATGAGCGATg                                                      >  2:1603925/1‑100 (MQ=255)
                                            gCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTc                                                   >  2:2012849/1‑100 (MQ=255)
                                             cTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCg                                                  >  1:3349039/1‑100 (MQ=255)
                                             cTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCg                                                  >  1:863939/1‑100 (MQ=255)
                                              tCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCgc                                                 >  1:3618050/1‑99 (MQ=255)
                                               cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTc                                                >  2:2292244/1‑100 (MQ=255)
                                               cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTc                                                <  2:2689671/100‑1 (MQ=255)
                                               cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTc                                                >  2:283821/1‑100 (MQ=255)
                                                gTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCt                                               <  1:2712647/100‑1 (MQ=255)
                                                gTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCt                                               >  2:3398696/1‑100 (MQ=255)
                                                  gatgaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGa                                             >  2:61882/1‑100 (MQ=255)
                                                   atgaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAg                                            <  2:2420795/100‑1 (MQ=255)
                                                   atgaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAg                                            >  2:2384893/1‑100 (MQ=255)
                                                     gaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTg                                          <  1:2858798/100‑1 (MQ=255)
                                                     gaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTg                                          <  1:3506176/100‑1 (MQ=255)
                                                       aGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGaa                                        >  1:1711413/1‑100 (MQ=255)
                                                        gCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCACCAACCAGCTGCGTGTGGTAATGAGCGTTGCTCGTCTGCGTGAAg                                       <  2:629204/100‑1 (MQ=255)
                                                         ccATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAgc                                      >  2:2942689/1‑100 (MQ=255)
                                                          cATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAgcg                                     >  2:144570/1‑100 (MQ=255)
                                                              gCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTg                                 <  2:1420415/100‑1 (MQ=255)
                                                               cGTATCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGa                                >  2:2658417/1‑100 (MQ=255)
                                                                 tATCTGAGCAAGCGCCATCCGGACCTGGTGATGCGCCGCCAGGGCAGCAACCTGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAat                              <  1:824069/100‑1 (MQ=255)
                                                                  aTCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAata                             <  1:3157080/100‑1 (MQ=255)
                                                                  aTCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAata                             >  1:549596/1‑100 (MQ=255)
                                                                  aTCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAata                             >  1:3266285/1‑100 (MQ=255)
                                                                  aTCTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCCCGTCTGAGTGAAGCGCGTGAata                             >  2:1014338/1‑100 (MQ=255)
                                                                    cTGAGCAAGCGCCATCCGGACCTGGTGGTTAGCAGGCAGAGCCGCAACTAGATGCTTAGAGTAATGAGCGATGCTGGTCTGAGCGCAGCGCGTGAATATg                           <  2:821786/100‑1 (MQ=255)
                                                                    cTGAGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATCTg                           >  2:2139179/1‑100 (MQ=255)
                                                                    cTGAGCAAGCGCCATCCGGACCTGGCGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATg                           >  2:3066277/1‑100 (MQ=255)
                                                                     tGAGCAAGCGGCATGCGGTCCTGGTGATGAACAGCCAGCGCAGCAACCAGCGGCGTCCGGTAATGAGCGATGCTCGTCTGCGTGGAGCGCGCCAATATGc                          <  2:978977/100‑1 (MQ=255)
                                                                       aGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCgg                        >  1:1075287/1‑100 (MQ=255)
                                                                       aGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCgg                        <  1:1626447/100‑1 (MQ=255)
                                                                       aGCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCgg                        <  2:1795371/100‑1 (MQ=255)
                                                                        gCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGt                       <  1:1982576/100‑1 (MQ=255)
                                                                        gCAAGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGGGAATATGCGGt                       >  2:2719988/1‑100 (MQ=255)
                                                                          aaGCGCCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgc                     <  1:1588600/100‑1 (MQ=255)
                                                                            gcgcCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcag                   <  1:1704768/100‑1 (MQ=255)
                                                                            gcgcCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcag                   <  1:1916836/100‑1 (MQ=255)
                                                                            gcgcCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcag                   <  1:1916858/100‑1 (MQ=255)
                                                                             cgcCATCCGGACCTGTTGATGAGCAGCCAGGTCATCAACCAGCTGCGTGCGGTAATAAGCGATGCTCGTCTGAGTAAAGCTCGGTAATATTCGGTgcagc                  <  2:808536/100‑1 (MQ=255)
                                                                              gcCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcagca                 <  2:3214981/100‑1 (MQ=255)
                                                                              gcCATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcagca                 <  1:2876689/100‑1 (MQ=255)
                                                                               ccATCCGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGcagcag                >  2:1659332/1‑100 (MQ=255)
                                                                                 ctCCGGACCTGGGGATGAGCAGCCGGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGAGGCGCGGCTGAGTGAAGCGGGTTTATATGCGGTGCCGCAGaa              >  2:3865840/2‑100 (MQ=255)
                                                                                 aTCCGGACCTGGTGATGAGCAGCCAGGGCAGCAAACAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGaa              <  1:3489779/100‑1 (MQ=255)
                                                                                    cGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACAt           >  1:453233/1‑100 (MQ=255)
                                                                                    cGGACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACAt           <  2:1059912/100‑1 (MQ=255)
                                                                                     ggACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACAtt          <  2:1330109/100‑1 (MQ=255)
                                                                                      gACCTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTa         <  1:638552/100‑1 (MQ=255)
                                                                                        ccTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGGAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAat       >  1:3875617/1‑100 (MQ=255)
                                                                                         cTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAata      >  2:2504915/1‑100 (MQ=255)
                                                                                         cTGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCACGTCTGAGTGAAGCGCGAGAATATGCGGTGCAGCAGAACATTAata      >  2:1617561/1‑100 (MQ=255)
                                                                                          tGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAatat     <  1:2761869/100‑1 (MQ=255)
                                                                                          tGGTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAatat     <  1:2628625/100‑1 (MQ=255)
                                                                                            gTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATcc   >  2:680390/1‑100 (MQ=255)
                                                                                            gTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATcc   >  2:854361/1‑100 (MQ=255)
                                                                                            gTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGAGAATATGCGGTGCAGCAGAACATTAATATcc   <  2:1363502/100‑1 (MQ=255)
                                                                                            gTGATGAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCGCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATcc   >  2:1814752/1‑100 (MQ=255)
                                                                                             tgatgaGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCt  >  1:1498226/1‑100 (MQ=255)
                                                                                             tgatgaGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCt  >  2:2495936/1‑100 (MQ=255)
                                                                                                 |                                                                                               
AAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCT  >  NC_000913/428155‑428347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A