Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,340	A→C	I225S (ATC→AGC)	tsx ←	nucleoside channel, receptor of phage T6 and colicin K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,340	0	A	C	97.9%	509.9 / ‑1.7	145	I225S (ATC→AGC)	tsx	nucleoside channel, receptor of phage T6 and colicin K
Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (63/79); total (65/80)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

AACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGC  >  NC_000913/431245‑431435

 aCGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCg                                                                                            <  1:2023641/100‑1 (MQ=255)

 aCGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCg                                                                                            <  1:487730/100‑1 (MQ=255)

    aGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTa                                                                                         >  1:3693710/1‑100 (MQ=255)

     gCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTAc                                                                                        >  1:2876610/1‑100 (MQ=255)

     gCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTAc                                                                                        <  1:2207044/100‑1 (MQ=255)

       tACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCg                                                                                      <  1:2790242/100‑1 (MQ=255)

          taaagagTAGTGCCAGTGATCGTAGTTCAGAGCCAGATTATGGCTGGAAGCGATAGGGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTg                                                                                   <  2:1982645/97‑1 (MQ=255)

          gACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTg                                                                                   >  1:1121117/1‑100 (MQ=255)

          gACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTg                                                                                   <  1:1115818/100‑1 (MQ=255)

           aCAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGt                                                                                  <  1:1331293/100‑1 (MQ=255)

           aCAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGGTATTAGTACGGGTCTTAATACCGTTGCTTGCGGTACCGCTga                                                                                  >  1:2014196/1‑99 (MQ=255)

            cAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTc                                                                                 <  1:2059451/100‑1 (MQ=255)

              gagTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCGGTCAt                                                                               <  2:3605191/100‑1 (MQ=255)

               agtagtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATc                                                                              >  2:1243215/1‑100 (MQ=255)

               agtagtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGCGTCTTAATCCAGTTGCTTGCGTTACCGCTGCCATc                                                                              <  2:2198596/100‑1 (MQ=255)

                gtagtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcc                                                                             <  1:3044528/100‑1 (MQ=255)

                gtagtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcc                                                                             <  1:3809521/100‑1 (MQ=255)

                gtagtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcc                                                                             <  1:963749/100‑1 (MQ=255)

                gtagtGCCAGTCATCGTAGTTCAGAGCCAGAATATGGCTGTAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTCACCGCTGTCATcc                                                                             <  1:3598569/100‑1 (MQ=255)

                  agtGCCAGTGATCGTAGTTCAGAGGCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcccc                                                                           <  2:934512/100‑1 (MQ=255)

                  agtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcccc                                                                           <  2:3341428/100‑1 (MQ=255)

                  agtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcccc                                                                           <  1:2814277/100‑1 (MQ=255)

                  agtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATcccc                                                                           <  1:1348099/100‑1 (MQ=255)

                   gtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCt                                                                          <  1:238344/100‑1 (MQ=255)

                       cAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAAt                                                                      >  2:1919235/1‑100 (MQ=255)

                       cAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAAt                                                                      >  2:3062579/1‑100 (MQ=255)

                        aGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATc                                                                     >  1:3092944/1‑100 (MQ=255)

                           gATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGa                                                                  <  2:2441915/100‑1 (MQ=255)

                           gATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGa                                                                  >  1:1693190/1‑100 (MQ=255)

                           gATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGa                                                                  <  1:3495241/100‑1 (MQ=255)

                             tCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAAc                                                                <  2:1354235/100‑1 (MQ=255)

                             tCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAAc                                                                <  1:4059643/100‑1 (MQ=255)

                               ttAGTTCAGAGCCAGAATAGGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAAGCGGAAccc                                                              <  2:1834942/99‑1 (MQ=255)

                                tAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAAcccc                                                             <  2:1789681/100‑1 (MQ=255)

                                  gTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAg                                                           <  2:2798972/100‑1 (MQ=255)

                                  gTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAg                                                           >  1:1173544/1‑100 (MQ=255)

                                    tCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTc                                                         <  2:2347708/100‑1 (MQ=255)

                                       gagCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGaa                                                      >  2:1277863/1‑100 (MQ=255)

                                       gagCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGaa                                                      <  2:2701596/100‑1 (MQ=255)

                                         gCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATAGCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGt                                                    <  2:3615002/100‑1 (MQ=255)

                                         gCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGt                                                    >  2:2179084/1‑100 (MQ=255)

                                           cAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTg                                                  >  1:3396102/1‑100 (MQ=255)

                                            aGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTgg                                                 <  2:1971872/100‑1 (MQ=255)

                                            aGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTgg                                                 <  2:1203969/100‑1 (MQ=255)

                                            aGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTgg                                                 >  2:1466997/1‑100 (MQ=255)

                                            aGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTgg                                                 >  1:3094991/1‑100 (MQ=255)

                                             gAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGt                                                >  2:258619/1‑100 (MQ=255)

                                               atatGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGa                                              <  1:3018536/100‑1 (MQ=255)

                                               atatGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGa                                              <  1:2797289/100‑1 (MQ=255)

                                                tatGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGaa                                             <  1:3491370/100‑1 (MQ=255)

                                                  tGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGc                                           <  1:3212215/100‑1 (MQ=255)

                                                  tGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGc                                           <  1:3209994/100‑1 (MQ=255)

                                                  tGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGc                                           >  1:2251523/1‑100 (MQ=255)

                                                  tGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGc                                           >  2:2916679/1‑100 (MQ=255)

                                                    gCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCg                                         >  1:1465562/1‑100 (MQ=255)

                                                    gCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCg                                         >  1:1048485/1‑100 (MQ=255)

                                                    gCTGGAAGCGATAGAGATAAGAGTACGGGTCGTAATACCGCGTCTTGCGCTATCACGGTCATCCCCTAAACCGGACGCCCGGTGTCTGTTGGTGAAGCCg                                         >  2:489899/1‑100 (MQ=255)

                                                     atGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGa                                        <  1:818354/99‑1 (MQ=255)

                                                       ggAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATg                                      >  1:2861708/1‑100 (MQ=255)

                                                       ggAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATg                                      >  1:267171/1‑100 (MQ=255)

                                                        gAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGt                                     >  2:936326/1‑100 (MQ=255)

                                                         aaGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTa                                    <  1:1396602/100‑1 (MQ=255)

                                                          gatcttAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAg                                   <  2:3203733/95‑1 (MQ=255)

                                                          aGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAg                                   <  1:1799494/100‑1 (MQ=255)

                                                           gCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGc                                  <  1:572475/100‑1 (MQ=255)

                                                             gATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTc                                <  2:14392/100‑1 (MQ=255)

                                                             gATAGAGTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTc                                >  1:602051/1‑100 (MQ=255)

                                                                 gagTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCt                            >  1:3926933/1‑100 (MQ=255)

                                                                 gagTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCt                            >  1:892130/1‑100 (MQ=255)

                                                                  agTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTg                           >  2:1379654/1‑100 (MQ=255)

                                                                  agTTATTAGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTg                           >  1:1269457/1‑100 (MQ=255)

                                                                    ttattaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGCACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGAc                         <  2:1693868/100‑1 (MQ=255)

                                                                    ttattaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGAc                         >  2:1528782/1‑100 (MQ=255)

                                                                     tattaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGACCCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGAcc                        <  2:1724139/100‑1 (MQ=255)

                                                                     tattaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTTGAAGTTGGTGAAGCCGATGTAGGTCAGCTGaac                        >  2:3439306/1‑98 (MQ=255)

                                                                     tattaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGAcc                        >  1:156067/1‑100 (MQ=255)

                                                                      attaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCg                       <  1:2890673/100‑1 (MQ=255)

                                                                      attaGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCg                       >  1:2862586/1‑100 (MQ=255)

                                                                        taGTACGGGTCTTAATACCGTTGCTTGCGTTACCGTTGAGATCCCGTAAATCGGCATCTCATTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGcc                     >  2:728494/1‑100 (MQ=255)

                                                                        taGTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGACGTTGGTGAAGCCGATGTAGCTCAGCTGACCGcc                     >  1:1403111/1‑100 (MQ=255)

                                                                          gTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGcccc                   <  2:1277909/100‑1 (MQ=255)

                                                                          gTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGcccc                   >  1:3542927/1‑100 (MQ=255)

                                                                          gTACGGGTCTTAATACCGTTGCTTGCGTTACCGCTGGCATTCCCTAAGTCGGGACCCCTGTCGAAGGTGGTGAAGCCGATGTAGCTCAGCTGACCGcccc                   <  2:544354/100‑1 (MQ=255)

                                                                             cGGGTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCcaca                >  1:755942/1‑100 (MQ=255)

                                                                               ggTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGa              >  2:3001333/1‑100 (MQ=255)

                                                                               ggTCATAATACAGTTGCTGGCGTGACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGa              <  2:386004/100‑1 (MQ=255)

                                                                                gTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGAt             >  2:3031745/1‑100 (MQ=255)

                                                                                gTCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGAt             >  1:1678960/1‑100 (MQ=255)

                                                                                 tCTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATc            <  2:3804730/100‑1 (MQ=255)

                                                                                  cTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCg           <  2:3244263/100‑1 (MQ=255)

                                                                                  cTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCg           >  1:3734669/1‑100 (MQ=255)

                                                                                  cTTAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCg           <  1:897195/100‑1 (MQ=255)

                                                                                    tAATACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGt         >  2:1889073/1‑100 (MQ=255)

                                                                                      aTACCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTaa       >  2:793801/1‑100 (MQ=255)

                                                                                        aCCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATc     >  1:1701350/1‑100 (MQ=255)

                                                                                        aCCGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATc     <  1:1852908/100‑1 (MQ=255)

                                                                                         ccGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCg    >  2:88088/1‑100 (MQ=255)

                                                                                         ccGTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCg    >  1:2527000/1‑100 (MQ=255)

                                                                                           gTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGc  >  2:868742/1‑100 (MQ=255)

                                                                                           gTTGCTTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGCTGAAGCCGATGTATCTCCGCTCACCGCCCCACGTATCGGAAATCgga  >  2:3724835/1‑99 (MQ=255)

AACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGC  >  NC_000913/431245‑431435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A