Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,566	A→C	L48W (TTG→TGG)	tomB ←	Hha toxicity attenuator; conjugation‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,566	0	A	C	97.6%	560.6 / ‑5.4	166	L48W (TTG→TGG)	tomB	Hha toxicity attenuator; conjugation‑related protein
Reads supporting (aligned to +/- strand): ref base A (3/1); new base C (70/92); total (73/93)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

CAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACT  >  NC_000913/480469‑480665

     atTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAg                                                                                              >  2:3635689/1‑100 (MQ=255)

      ttCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAgg                                                                                             <  1:343228/100‑1 (MQ=255)

      tcttccgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAgg                                                                                             <  2:505142/95‑1 (MQ=255)

       tcgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGt                                                                                            <  2:3002367/100‑1 (MQ=255)

        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                           <  2:2669603/100‑1 (MQ=255)

        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                           >  1:1489937/1‑100 (MQ=255)

        cgtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGtt                                                                                           >  1:3269796/1‑100 (MQ=255)

         ntcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTg                                                                                          >  1:3260714/2‑100 (MQ=255)

         gtcgATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTg                                                                                          <  2:3343603/100‑1 (MQ=255)

          tcgAGCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGa                                                                                         <  1:3424543/100‑1 (MQ=255)

               cTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGcc                                                                                    >  2:2942370/1‑100 (MQ=255)

                tGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCg                                                                                   >  1:2319149/1‑100 (MQ=255)

                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  1:2502421/1‑100 (MQ=255)

                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  1:363024/1‑100 (MQ=255)

                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  1:3864124/1‑100 (MQ=255)

                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  <  2:1351084/100‑1 (MQ=255)

                 gCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGa                                                                                  >  2:1464063/1‑100 (MQ=255)

                  cTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAg                                                                                 <  1:3993416/100‑1 (MQ=255)

                   tCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAgg                                                                                <  1:21164/100‑1 (MQ=255)

                    cAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGt                                                                               >  2:2504645/1‑100 (MQ=255)

                     aaTGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGtt                                                                              <  2:3912852/100‑1 (MQ=255)

                     aaTGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGtt                                                                              >  1:2414815/1‑100 (MQ=255)

                      aTGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTg                                                                             <  2:3876203/100‑1 (MQ=255)

                       tGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTgg                                                                            >  2:2521851/1‑100 (MQ=255)

                        gcgCTTATTGTCTTCATTATACTTAATTTTTTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATGCCACTGGAGGTTGATCGCCGAGGTTggg                                                                           <  1:1730033/98‑1 (MQ=255)

                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGt                                                                          <  1:2916875/100‑1 (MQ=255)

                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGt                                                                          <  1:2312527/100‑1 (MQ=255)

                         aGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGt                                                                          >  2:122245/1‑100 (MQ=255)

                          gCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTc                                                                         <  2:1154140/100‑1 (MQ=255)

                            ttATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGt                                                                       >  2:1901630/1‑100 (MQ=255)

                             tATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGtt                                                                      <  1:2604492/100‑1 (MQ=255)

                              aTTGTCTTCATTATACTTGATTTTGTAATTAAGTGCGGAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGttt                                                                     <  2:334974/100‑1 (MQ=255)

                                tGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAc                                                                   >  2:2180372/1‑100 (MQ=255)

                                tGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAc                                                                   >  2:321809/1‑100 (MQ=255)

                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  <  1:3349399/100‑1 (MQ=255)

                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  >  1:3394424/1‑100 (MQ=255)

                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  <  1:3274337/100‑1 (MQ=255)

                                 gTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAcc                                                                  >  1:310420/1‑100 (MQ=255)

                                  tCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTAccc                                                                 <  1:1354117/100‑1 (MQ=255)

                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                                <  2:2801209/100‑1 (MQ=255)

                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                                <  1:1555275/100‑1 (MQ=255)

                                   cTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCa                                                                >  1:3691410/1‑100 (MQ=255)

                                     tCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGc                                                              <  2:1411052/100‑1 (MQ=255)

                                          ataCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGa                                                         <  1:824620/100‑1 (MQ=255)

                                           taCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAt                                                        <  1:1986428/100‑1 (MQ=255)

                                            aCTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGAtt                                                       <  2:3517296/100‑1 (MQ=255)

                                             cTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTg                                                      >  1:1957421/1‑100 (MQ=255)

                                                aaTTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCtt                                                   <  1:894230/100‑1 (MQ=255)

                                                 aTTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCttt                                                  <  1:2598511/100‑1 (MQ=255)

                                                   tttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttct                                                <  1:3498872/100‑1 (MQ=255)

                                                    ttGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttctt                                               <  1:436599/100‑1 (MQ=255)

                                                     tGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTttcttc                                              <  1:3037431/100‑1 (MQ=255)

                                                      gTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCa                                             >  2:3091317/1‑100 (MQ=255)

                                                       tAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCaa                                            >  2:1450505/1‑100 (MQ=255)

                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                          <  2:2711072/100‑1 (MQ=255)

                                                         aTTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAgg                                          >  1:2640877/1‑100 (MQ=255)

                                                             agggCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTg                                      >  2:3214506/4‑100 (MQ=255)

                                                              gTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGc                                     >  1:2279055/1‑100 (MQ=255)

                                                               tGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCTATGATTGCTTTCTTCAAGGTTTGCa                                    <  1:1170013/100‑1 (MQ=255)

                                                               tGCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCa                                    >  2:690818/1‑100 (MQ=255)

                                                                gCGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCaa                                   >  1:3936028/1‑100 (MQ=255)

                                                                 cGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAg                                  <  2:794951/100‑1 (MQ=255)

                                                                 cGAAGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAg                                  >  1:2543786/1‑100 (MQ=255)

                                                                   aaGGTCGCAATATGCTCAATGAGTTCATTCCGCTGGAGGTTGTTCGCCGAGGTTGGGTCGTTTACCCAGCGATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  2:1471361/100‑1 (MQ=255)

                                                                   aaGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  2:1327393/100‑1 (MQ=255)

                                                                   aaGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  2:2026847/100‑1 (MQ=255)

                                                                   aaGGTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGc                                <  1:2209738/100‑1 (MQ=255)

                                                                     ggTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAg                              <  2:2283736/100‑1 (MQ=255)

                                                                      gTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCACGGCAGt                             >  1:487070/1‑100 (MQ=255)

                                                                      gTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGt                             >  2:2080341/1‑100 (MQ=255)

                                                                      gTCGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGt                             <  2:2463962/100‑1 (MQ=255)

                                                                        cGCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCa                           <  2:2105602/100‑1 (MQ=255)

                                                                         gCAATATGCTCAATCGGTTCATGCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt                          <  2:861480/100‑1 (MQ=255)

                                                                         gCAATATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCAt                          >  1:1023443/1‑100 (MQ=255)

                                                                           aaTATGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGa                        >  2:1660906/1‑100 (MQ=255)

                                                                               tGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACa                    >  1:857308/1‑100 (MQ=255)

                                                                               tGCTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGAGACa                    <  1:3138576/100‑1 (MQ=255)

                                                                                 cTCAATCAGTTCATTCCGTTGGAGGTTGATCGCCGAGGTGGGGTCGTTTAGCCAGCCATGATTTCTTTCTTCAAGGTTTTCAAGGCAGTCATGATACAgg                  >  2:2870763/1‑100 (MQ=255)

                                                                                 cTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg                  <  1:2924169/100‑1 (MQ=255)

                                                                                 cTCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAgg                  <  2:1038478/100‑1 (MQ=255)

                                                                                  tCAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAggg                 <  2:825081/100‑1 (MQ=255)

                                                                                   cAATCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGt                >  2:1553916/1‑100 (MQ=255)

                                                                                     aTCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt              <  2:1003492/100‑1 (MQ=255)

                                                                                     aTCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGttt              <  1:787462/100‑1 (MQ=255)

                                                                                      tCAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTc             <  1:2467597/100‑1 (MQ=255)

                                                                                       ctgTTCTTTAAACTGGAGGTTGATCGTCGTGGTTGGGTAGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            <  1:763894/98‑1 (MQ=255)

                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            >  2:1134241/1‑100 (MQ=255)

                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            <  1:2137242/100‑1 (MQ=255)

                                                                                       cAGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTca            >  2:938621/1‑100 (MQ=255)

                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  1:1589228/100‑1 (MQ=255)

                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  1:1688861/100‑1 (MQ=255)

                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  1:1842937/100‑1 (MQ=255)

                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcac           <  2:98344/100‑1 (MQ=255)

                                                                                        aGTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTAGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCACGGCAGTCATGATACAGGGTTTcac           >  2:382435/1‑100 (MQ=255)

                                                                                         gTTCATTCCACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTcaca          >  1:2969045/1‑100 (MQ=255)

                                                                                           tcattccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACaga        <  1:407453/100‑1 (MQ=255)

                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa     >  1:3728497/1‑100 (MQ=255)

                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa     >  2:1848520/1‑100 (MQ=255)

                                                                                              ttccACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGaa     <  2:2354045/100‑1 (MQ=255)

                                                                                                 aaCTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACt  <  2:304997/100‑1 (MQ=255)

CAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACT  >  NC_000913/480469‑480665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A