Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 488,082 T→G I516S (ATT→AGT)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913488,0820TG96.1% 413.2 / 5.6 127I516S (ATT→AGT) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base G (59/63);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 127 total aligned reads displayed.

AAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAA  >  NC_000913/487985‑488178
                                                                                                 |                                                                                                
aaaGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTg                                                                                                <  1:726208/100‑1 (MQ=255)
    aTGCATTTAAGTCGATGGAACTCCCGGTGAACTGGCCCCAAGCATGGCCCGCCGTTTTTGTCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCgg                                                                                            <  2:2611727/100‑1 (MQ=255)
    aTGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCgg                                                                                            <  2:2950012/100‑1 (MQ=255)
    aTGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCgg                                                                                            >  1:2650757/1‑100 (MQ=255)
    aTGAATTTAAGTCGATGAAAATCACGGTGAACCGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCCCTGGCTCGCCGCTGCTGTTGAGTGCCgg                                                                                            >  2:40607/1‑100 (MQ=255)
      gAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGCCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTTAGTGCCGGGc                                                                                          <  2:873404/100‑1 (MQ=255)
       aaTTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCt                                                                                         >  2:3885147/1‑100 (MQ=255)
       aaTTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTATTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGCGCCGGGCt                                                                                         >  2:2792796/1‑100 (MQ=255)
          ttAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGAt                                                                                      <  1:1322654/100‑1 (MQ=255)
           taggTCGATGAAGGTCAGGGTGAGCTGGCACAAAGCGTGGCCCGCCGTTTTTATCGCTGTGCTCGGTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATc                                                                                     <  2:1967347/97‑1 (MQ=255)
           tAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATc                                                                                     >  2:1008795/1‑100 (MQ=255)
            aaGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATcc                                                                                    >  1:2743207/1‑100 (MQ=255)
            aaGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATcc                                                                                    <  1:3432490/100‑1 (MQ=255)
             aGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCa                                                                                   >  2:718132/1‑100 (MQ=255)
               tCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACt                                                                                 <  1:2633921/100‑1 (MQ=255)
               tCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACt                                                                                 <  1:2805447/100‑1 (MQ=255)
                cGATGAAAATCACGGTGAAGTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTg                                                                                >  2:1062127/1‑100 (MQ=255)
                cGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTg                                                                                >  2:205381/1‑100 (MQ=255)
                cGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTg                                                                                >  2:905774/1‑100 (MQ=255)
                cGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTCGAGTGCCGGGCTGATCCACTg                                                                                <  1:3856321/100‑1 (MQ=255)
                 gATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCagatc                                                                               >  1:2761590/1‑96 (MQ=255)
                 gATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTgg                                                                               >  2:674356/1‑100 (MQ=255)
                      aaaTCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCt                                                                          <  2:2993395/100‑1 (MQ=255)
                      aaaTCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCt                                                                          <  2:3853858/100‑1 (MQ=255)
                       aaTCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTg                                                                         <  1:334510/100‑1 (MQ=255)
                          cACGGTTAACTGGCCAAAAGCCTGGCCCGCCGTTTTTATCGCTTGCCTCGCTGGTTGGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggc                                                                      <  2:1391594/100‑1 (MQ=255)
                          cACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGggc                                                                      >  2:576978/1‑100 (MQ=255)
                          cACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggc                                                                      <  1:2975154/100‑1 (MQ=255)
                          cACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggc                                                                      <  1:2127571/100‑1 (MQ=255)
                           agggggAACTGGCAAAAAGCCCGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGggct                                                                     <  2:2131998/95‑1 (MQ=255)
                           aCGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggct                                                                     <  2:3359815/100‑1 (MQ=255)
                           aCGGTGAACTGGCAAAAAGCCTGACCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGCTCCACTGGCGTCTGggct                                                                     >  2:1801534/1‑100 (MQ=255)
                            cGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggctg                                                                    >  2:2874397/1‑100 (MQ=255)
                             ggTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggctgg                                                                   <  1:1030341/100‑1 (MQ=255)
                             ggTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggctgg                                                                   >  2:595690/1‑100 (MQ=255)
                              gTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggctggc                                                                  >  1:1040227/1‑100 (MQ=255)
                              gTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggctggc                                                                  <  2:1967569/100‑1 (MQ=255)
                              gTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGggctggc                                                                  <  2:1313142/100‑1 (MQ=255)
                                 aaCTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGa                                                               >  2:2586053/1‑100 (MQ=255)
                                 aaCTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGa                                                               >  2:2097360/1‑100 (MQ=255)
                                    tGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAg                                                            <  1:1937543/100‑1 (MQ=255)
                                    tGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAg                                                            <  1:3407007/100‑1 (MQ=255)
                                     ggCAAAAAGCCTGGCCCGCCGTTTTAATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGCTCCACTGGCGTCTGGGCTGGCTGCAAGc                                                           >  2:3185371/1‑100 (MQ=255)
                                       cAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGt                                                         >  2:1265553/1‑100 (MQ=255)
                                       cAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGt                                                         >  1:3782936/1‑100 (MQ=255)
                                        aaaaaGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTa                                                        <  1:1295866/100‑1 (MQ=255)
                                        aaaaaGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTa                                                        <  2:2945993/100‑1 (MQ=255)
                                        aaaaaGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTa                                                        <  2:2509526/100‑1 (MQ=255)
                                        aaaaaGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCGGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTa                                                        <  2:3324282/100‑1 (MQ=255)
                                          aaaGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATc                                                      <  1:2626135/100‑1 (MQ=255)
                                             gCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATcaac                                                   <  1:751553/100‑1 (MQ=255)
                                             gCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATcaac                                                   >  1:1246351/1‑100 (MQ=255)
                                             gCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATcaac                                                   >  2:1677654/1‑100 (MQ=255)
                                               cTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATcaacaa                                                 <  2:2064941/100‑1 (MQ=255)
                                                  gCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACaaaaa                                              >  1:2815096/1‑100 (MQ=255)
                                                   cccGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAAc                                             >  1:2751401/1‑100 (MQ=255)
                                                    ccgccgTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACt                                            <  2:1137650/100‑1 (MQ=255)
                                                    ccgccgTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACt                                            <  1:3935982/100‑1 (MQ=255)
                                                     cgccgTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGTTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTg                                           >  2:1993119/1‑100 (MQ=255)
                                                           ttttATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTcc                                     <  2:739252/100‑1 (MQ=255)
                                                           ttttATCCCATTCCTCGCTGGTTTGCCGCGGCTGTTGAGTGCCGGGCTGATCCACTAGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTcc                                     >  2:3296357/1‑100 (MQ=255)
                                                            tttATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCg                                    <  2:2871135/100‑1 (MQ=255)
                                                             ttATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGc                                   <  1:2222327/100‑1 (MQ=255)
                                                              tATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCt                                  >  2:431301/1‑100 (MQ=255)
                                                                 cGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCtgtg                               <  2:2795356/100‑1 (MQ=255)
                                                                 cGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGAGCTGTTCCACTGGCGACTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTCCCGCtgtg                               <  2:86237/100‑1 (MQ=255)
                                                                  gCTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTTGCTGAAAGCGTATCAACAAAACCTGGCTTCCGCTGTgg                              >  1:3991865/1‑100 (MQ=255)
                                                                   cTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTggg                             <  1:1034847/100‑1 (MQ=255)
                                                                   cTTTCCTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTggg                             <  1:3482868/100‑1 (MQ=255)
                                                                       ccTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTcc                         >  1:18495/1‑100 (MQ=255)
                                                                        cTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTccc                        >  1:1223295/1‑100 (MQ=255)
                                                                        cTCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTccc                        >  1:219234/1‑100 (MQ=255)
                                                                         tCGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCt                       <  1:752606/100‑1 (MQ=255)
                                                                          cGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTg                      <  2:2074373/100‑1 (MQ=255)
                                                                          cGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTg                      >  2:3056636/1‑100 (MQ=255)
                                                                          cGCTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTg                      <  2:56528/100‑1 (MQ=255)
                                                                            cTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCTCTGGCGTCTGGTCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCg                    <  1:1681223/100‑1 (MQ=255)
                                                                            cTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCg                    <  2:1018607/100‑1 (MQ=255)
                                                                            cTGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCg                    <  2:3711388/100‑1 (MQ=255)
                                                                             tGGTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGt                   <  2:3693179/100‑1 (MQ=255)
                                                                              ggTTTGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTa                  >  2:3189650/1‑100 (MQ=255)
                                                                                tttGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAAc                >  1:3001104/1‑100 (MQ=255)
                                                                                 ttGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACg               <  2:3952370/100‑1 (MQ=255)
                                                                                 ttGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACg               <  1:3634380/100‑1 (MQ=255)
                                                                                 ttGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACg               <  1:1093355/100‑1 (MQ=255)
                                                                                  tGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACTAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGa              >  1:3838242/1‑100 (MQ=255)
                                                                                  tGCCGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGa              >  2:1849304/1‑100 (MQ=255)
                                                                                    ccGCTGCTGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAca            >  1:3385042/1‑100 (MQ=255)
                                                                                      gctgctGTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagc          >  2:679578/1‑100 (MQ=255)
                                                                                       ctgctgTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagcc         <  2:2716175/100‑1 (MQ=255)
                                                                                         gctgTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagccag       >  2:3258529/1‑100 (MQ=255)
                                                                                          ctgTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagccagc      >  1:2489600/1‑100 (MQ=255)
                                                                                           tgTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCt     >  2:2178696/1‑100 (MQ=255)
                                                                                           tgTTAAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCt     >  2:3386711/1‑100 (MQ=255)
                                                                                            gTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTc    >  1:3486934/1‑100 (MQ=255)
                                                                                            gTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTc    <  1:1630903/100‑1 (MQ=255)
                                                                                            gTTGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTc    >  2:2286313/1‑100 (MQ=255)
                                                                                              tGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCGGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCaa  >  2:1818404/1‑100 (MQ=255)
                                                                                              tGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCaa  >  2:3957986/1‑100 (MQ=255)
                                                                                              tGAGTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCaa  >  2:2339352/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAA  >  NC_000913/487985‑488178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A