Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 970,734 A→C N21T (AAC→ACC)  ycaR → peroxide and acid resistance protein, UPF0434 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913970,7340AC95.3% 480.4 / 9.0 149N21T (AAC→ACC) ycaRperoxide and acid resistance protein, UPF0434 family
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base C (72/70);  total (76/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 153 total aligned reads displayed.

TTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGTGCTG  >  NC_000913/970637‑970831
                                                                                                 |                                                                                                 
tttATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAccc                                                                                                 <  1:3654305/100‑1 (MQ=255)
tttATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAccc                                                                                                 <  1:300757/100‑1 (MQ=255)
 ttATGCATTCATAACGCGATCCATCTGGAGGAACTATGGATAATGCTCGGCCTGAAATCATTGCCTGCCCCCTTTGCAACGGAAAACTTTGGTATACCCa                                                                                                <  2:3810411/100‑1 (MQ=255)
 ttATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCa                                                                                                <  2:50596/100‑1 (MQ=255)
 ttATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCa                                                                                                <  2:3222441/100‑1 (MQ=255)
  tATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAg                                                                                               >  1:699030/1‑100 (MQ=255)
   aTGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGTCCGGTTTGCAACGGAAAACTTTGGTATACCCAgg                                                                                              <  1:2441926/100‑1 (MQ=255)
   aTGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAgg                                                                                              <  2:2935834/100‑1 (MQ=255)
    tGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATCCCCAGGa                                                                                             >  2:914496/1‑100 (MQ=255)
    tGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGa                                                                                             >  1:545048/1‑100 (MQ=255)
     gCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGaa                                                                                            >  2:1488924/1‑100 (MQ=255)
      cATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGaaa                                                                                           >  1:3513587/1‑100 (MQ=255)
       aTTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAgatcgg                                                                                          >  1:3450926/1‑95 (MQ=255)
       aTTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGaaaa                                                                                          <  1:2578383/100‑1 (MQ=255)
        ttCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGaacaa                                                                                         >  2:2903243/1‑100 (MQ=255)
        ttCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGaaaaa                                                                                         <  2:3841846/100‑1 (MQ=255)
         tCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAAc                                                                                        >  1:1879928/1‑100 (MQ=255)
         tCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAAc                                                                                        >  1:2622459/1‑100 (MQ=255)
         tCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAAc                                                                                        <  1:3107948/100‑1 (MQ=255)
         tCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAAc                                                                                        <  2:570130/100‑1 (MQ=255)
          cATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACa                                                                                       >  2:1740698/1‑100 (MQ=255)
           aTAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACaa                                                                                      >  1:1320777/1‑100 (MQ=255)
           aTAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACaa                                                                                      >  1:410362/1‑100 (MQ=255)
             aaCCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAaga                                                                                    <  2:1475812/100‑1 (MQ=255)
              aCCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAagag                                                                                   >  1:1357642/1‑100 (MQ=255)
                ccGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCt                                                                                 <  1:2320280/100‑1 (MQ=255)
                ccGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCt                                                                                 <  1:3948731/100‑1 (MQ=255)
                 cGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCtt                                                                                >  2:249118/1‑100 (MQ=255)
                 cGATCCATCTGGAGGAACTATGGATCATCGACTGCTTGAAATCATGGCCTGCCCGGTTTGCAACGGAGAACTTTGGTATACCCAGGAAACACAAGAGCtt                                                                                >  2:1355450/1‑100 (MQ=255)
                 cGATCCATCTGGAGGAACTATGAATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCtt                                                                                >  1:3412655/1‑100 (MQ=255)
                  gATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTa                                                                               <  1:2583015/100‑1 (MQ=255)
                   aTCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTAt                                                                              <  1:21073/100‑1 (MQ=255)
                    tCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTAtt                                                                             <  1:3659933/100‑1 (MQ=255)
                    tCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTAtt                                                                             <  1:1445153/100‑1 (MQ=255)
                    gataTCTGGAGCGACTATGGATAATCGTATGCTTGAAAACATTACCAGCCCGGTTTGCAACGGAAAACTTGAGAAAAAAAAAAAAAAACAAGAGCTTAtt                                                                             <  2:1384640/97‑1 (MQ=255)
                       aTCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGc                                                                          >  1:3903267/1‑100 (MQ=255)
                       aTCTGGAGGAACTATGGATCATCGTCTGCTTCAAATCATTGCATGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGc                                                                          >  1:3269259/1‑100 (MQ=255)
                       aTCGGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCAGTCCGCACTGCAACGGAAAACTTTTGTACACCCAGGAAAAACAAGAGCTTATTTGc                                                                          <  2:3984008/100‑1 (MQ=255)
                        tCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCa                                                                         <  1:704956/100‑1 (MQ=255)
                        tCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCa                                                                         >  1:1077914/1‑100 (MQ=255)
                        tCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCa                                                                         <  1:196125/100‑1 (MQ=255)
                          tGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCaaa                                                                       >  1:154099/1‑100 (MQ=255)
                           ggaggaACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAAc                                                                      >  1:2710700/1‑100 (MQ=255)
                           ggaggaACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACACAGGAAAAACAAGAGCTTATTTGCAAAc                                                                      >  1:831624/1‑100 (MQ=255)
                              gtaaCTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACCGAAAACTTTGGTATACCCAGTAAAAACAAGAGCTTATTTGCAAACTgg                                                                   <  2:3977483/98‑1 (MQ=255)
                              ggaACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTgg                                                                   <  1:2365764/100‑1 (MQ=255)
                              ggaACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTgg                                                                   <  2:793690/100‑1 (MQ=255)
                                aaCTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGAc                                                                 <  2:2231046/100‑1 (MQ=255)
                                aaCTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGAc                                                                 <  1:524971/100‑1 (MQ=255)
                                 aCTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACa                                                                >  1:3358827/1‑100 (MQ=255)
                                  cTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACaa                                                               >  1:1832187/1‑100 (MQ=255)
                                   tATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAAc                                                              >  2:3071220/1‑100 (MQ=255)
                                    aTGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAAcc                                                             <  2:3457309/100‑1 (MQ=255)
                                      ggATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTc                                                           >  2:333425/1‑100 (MQ=255)
                                       gATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCg                                                          >  1:54638/1‑100 (MQ=255)
                                       gATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCg                                                          <  2:1715335/100‑1 (MQ=255)
                                         tcatcGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCt                                                        >  1:1049957/1‑100 (MQ=255)
                                          catcGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCtt                                                       <  1:2599183/100‑1 (MQ=255)
                                           atcGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCttt                                                      >  2:1992188/1‑100 (MQ=255)
                                              gTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTccc                                                   <  1:333792/100‑1 (MQ=255)
                                               tCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCg                                                  <  2:2073161/100‑1 (MQ=255)
                                                 tGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCt                                                >  1:2207341/1‑100 (MQ=255)
                                                  gCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTg                                               >  1:3515120/1‑100 (MQ=255)
                                                  gCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTg                                               >  1:3206671/1‑100 (MQ=255)
                                                  gCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTg                                               <  1:1287464/100‑1 (MQ=255)
                                                  gCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTg                                               >  1:1468150/1‑100 (MQ=255)
                                                  gCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTg                                               >  2:1755521/1‑100 (MQ=255)
                                                   cTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGc                                              >  1:2350839/1‑100 (MQ=255)
                                                    ttGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCg                                             >  1:90423/1‑100 (MQ=255)
                                                    ttGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCg                                             <  1:121841/100‑1 (MQ=255)
                                                        aaTCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGAt                                         <  2:3470973/100‑1 (MQ=255)
                                                          tCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATgg                                       >  1:425904/1‑100 (MQ=255)
                                                          tCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATgg                                       <  1:1577503/100‑1 (MQ=255)
                                                           cATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGc                                      <  2:854442/100‑1 (MQ=255)
                                                             ttGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCAt                                    <  1:2482574/100‑1 (MQ=255)
                                                                cctgccCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTcc                                 <  1:3767439/100‑1 (MQ=255)
                                                                cctgccCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTcc                                 >  1:3668543/1‑100 (MQ=255)
                                                                 ctgccCGGTTTGCAACGGAAAAATTTGGTATCCGCAGGAAAAACAAGAGCTTACTAACAAACTGGACAACCTCACCTTCCCGATGAGTGACCGCATcccc                                >  2:3381899/1‑99 (MQ=255)
                                                                  tgccCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAg                               >  1:1254194/1‑100 (MQ=255)
                                                                   gccCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAgt                              >  2:2888357/1‑100 (MQ=255)
                                                                   gccCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAgt                              >  2:2549167/1‑100 (MQ=255)
                                                                    cccGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAgtg                             <  1:1807830/100‑1 (MQ=255)
                                                                    cccGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAgtg                             <  2:550443/100‑1 (MQ=255)
                                                                    cccGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAgtg                             <  2:1567686/100‑1 (MQ=255)
                                                                       ggTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTg                          >  2:2009933/1‑100 (MQ=255)
                                                                          ttGCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTg                       <  2:3157489/100‑1 (MQ=255)
                                                                          gtGCAACGGAAAACTTTGGTATTCCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTg                       <  1:2418257/99‑1 (MQ=255)
                                                                            gCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGa                     >  1:1168005/1‑100 (MQ=255)
                                                                            gCAACGGAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGa                     >  2:2236230/1‑100 (MQ=255)
                                                                                  gAAAACTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGa               <  2:1559829/100‑1 (MQ=255)
                                                                                   aaaaCTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGac              >  2:3495971/1‑99 (MQ=255)
                                                                                   aaaaCTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGaa              >  2:3319820/1‑100 (MQ=255)
                                                                                   aaaaCTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGaa              <  1:2677394/100‑1 (MQ=255)
                                                                                    aaaCTTTGGTATACCCCGGAAAAACAAGCGCTTGTTTGCAAACTGGACGACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAg             <  2:779432/100‑1 (MQ=255)
                                                                                     aaCTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGc            >  2:672712/1‑100 (MQ=255)
                                                                                       cTTTGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGccc          >  2:372856/1‑100 (MQ=255)
                                                                                        tttGGTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCcg         >  1:2785528/1‑100 (MQ=255)
                                                                                           ggTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGt      >  1:1969080/1‑100 (MQ=255)
                                                                                            gTATACCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGTg     >  2:1798237/1‑100 (MQ=255)
                                                                                             tataCCCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGTGc    <  2:453146/100‑1 (MQ=255)
                                                                                                 |                                                                                                 
TTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGTGCTG  >  NC_000913/970637‑970831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A