Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,093,771 A→C E299A (GAA→GCA)  ycdT → diguanylate cyclase, membrane‑anchored

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,093,7710AC97.6% 420.8 / ‑3.8 129E299A (GAA→GCA) ycdTdiguanylate cyclase, membrane‑anchored
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (59/65);  total (62/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TTTATGTGCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACGACACCTGGGGGCATCC  >  NC_000913/1093674‑1093870
                                                                                                  |                                                                                                   
tttATGTGCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCAc                                                                                                   >  2:1018782/1‑100 (MQ=255)
tttATGTGCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCAc                                                                                                   <  1:2951750/100‑1 (MQ=255)
    tgtgCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGAc                                                                                               <  1:983204/100‑1 (MQ=255)
      tgCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAg                                                                                             <  2:2466568/100‑1 (MQ=255)
      tgCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAg                                                                                             >  1:1485322/1‑100 (MQ=255)
      tgCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAg                                                                                             <  2:3303848/100‑1 (MQ=255)
       gCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGt                                                                                            >  1:3776790/1‑100 (MQ=255)
       gCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGt                                                                                            >  2:219563/1‑100 (MQ=255)
        ccATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGtt                                                                                           >  1:1633910/1‑100 (MQ=255)
         cATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTc                                                                                          >  1:419109/1‑100 (MQ=255)
         cATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTc                                                                                          <  1:1018707/100‑1 (MQ=255)
         cATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTc                                                                                          <  1:1326140/100‑1 (MQ=255)
          atatatTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCa                                                                                         <  2:164245/100‑1 (MQ=255)
           tatatTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCaa                                                                                        <  2:2783989/100‑1 (MQ=255)
           tatatTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCaa                                                                                        >  1:3771525/1‑100 (MQ=255)
             tatTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatc                                                                                      <  1:2782324/100‑1 (MQ=255)
              atTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatca                                                                                     >  1:3923186/1‑100 (MQ=255)
                 cAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagca                                                                                  >  1:1232535/1‑100 (MQ=255)
                 cAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagca                                                                                  >  1:1981787/1‑100 (MQ=255)
                 cAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagca                                                                                  >  1:1231775/1‑100 (MQ=255)
                 cAGTGCGCTACGAGTAACAAAGAACATAGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagca                                                                                  <  2:2105812/100‑1 (MQ=255)
                  aGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagcat                                                                                 <  2:1223753/100‑1 (MQ=255)
                  aGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCACTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagcat                                                                                 <  2:1534470/100‑1 (MQ=255)
                  aGTGCGCTACGAGTAACAAAGAACATTGCACAACGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGAACTGACAGTTCAatcagcat                                                                                 >  2:3596538/1‑100 (MQ=255)
                    tGCGCTACGAGGAACAAAGAACATTGCACATCGCTATTCCTTAACCAAGATATTTAACATAAATTATTTTTTTTAATGCACTGACAGTTTAatcagcatc                                                                                <  2:820565/100‑1 (MQ=255)
                      cgcTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAAGATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAatcagcatcagc                                                                             >  1:3940200/1‑100 (MQ=255)
                       gcTACGAGTAACAAAGAACATTGCACATCGCTATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCGCTGACAGGTCAATCAGCATCAGcc                                                                            <  2:2427369/100‑1 (MQ=255)
                       gcTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGcc                                                                            >  1:3028294/1‑100 (MQ=255)
                       gcTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGcc                                                                            >  1:346254/1‑100 (MQ=255)
                           cGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCaaa                                                                        >  2:1123567/1‑100 (MQ=255)
                            gAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCaaaa                                                                       >  1:3560506/1‑100 (MQ=255)
                              gTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCaaaaaa                                                                     <  2:712080/100‑1 (MQ=255)
                               tAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAAc                                                                    <  1:1221731/100‑1 (MQ=255)
                                aaCAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACg                                                                   >  2:2772625/1‑100 (MQ=255)
                                 aCAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGc                                                                  >  1:1944686/1‑100 (MQ=255)
                                  cAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGcc                                                                 >  2:1275351/1‑100 (MQ=255)
                                  cAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAA‑TA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCt                                                                >  1:2852611/1‑100 (MQ=255)
                                   aaaGAACATTGCACATCGCGATCCCTTAACGAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCt                                                                >  1:3090130/1‑100 (MQ=255)
                                   aaaGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCt                                                                <  1:2280133/100‑1 (MQ=255)
                                    aaGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCtt                                                               <  1:3280519/100‑1 (MQ=255)
                                      gAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTTTTTTTTTTTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTAt                                                             >  2:2781058/1‑100 (MQ=255)
                                       aaCATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTAtt                                                            >  2:3069753/1‑100 (MQ=255)
                                         cATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTTTTTTTTTTTCAATTCAATCACCATCAGCCCAAAACACGCTTTATTGc                                                          >  2:1862196/1‑100 (MQ=255)
                                         cATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGc                                                          <  1:1440770/100‑1 (MQ=255)
                                          aTTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCg                                                         >  1:1207818/1‑100 (MQ=255)
                                            tGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTc                                                       >  2:3890161/1‑100 (MQ=255)
                                            tGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTc                                                       <  1:945130/100‑1 (MQ=255)
                                            tGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTc                                                       <  1:2374176/100‑1 (MQ=255)
                                              cacaTCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCAt                                                     >  1:2290393/1‑100 (MQ=255)
                                               acaTCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATg                                                    <  2:478818/100‑1 (MQ=255)
                                               acaTCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGATCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATg                                                    >  1:3605924/1‑100 (MQ=255)
                                                caTCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGa                                                   >  1:2370887/1‑100 (MQ=255)
                                                caTCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGa                                                   >  1:3763507/1‑100 (MQ=255)
                                                 aTCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGAt                                                  >  2:175183/1‑100 (MQ=255)
                                                   cgcgATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTa                                                >  2:1516075/1‑100 (MQ=255)
                                                   cgcgAAACCTTAACCAATATAATTAACAGAAATTA‑TTTTTTTAATGAACTGACAGTTTAATCAAAAAAAAAAAAAAAAACGCCTTATTGCGTCATGATTa                                                <  2:3420214/100‑1 (MQ=255)
                                                    gcgATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTAt                                               <  1:2492264/100‑1 (MQ=255)
                                                    gcgATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTAt                                               <  1:1319069/100‑1 (MQ=255)
                                                     cgATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAATCGCCTTATTGCGTCATGATTATg                                              <  2:84201/100‑1 (MQ=255)
                                                     cgATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATg                                              <  1:1820038/100‑1 (MQ=255)
                                                     cgATCCCCTAACCAACATATTTAACGGAAATTA‑TTTTTTTAATGCACTGACACTTCAATCAGCATCATCCCAAAAAACGCCTTATTGCGCCATCATTATg                                              >  2:2257630/1‑100 (MQ=255)
                                                      gATCCCGCAACAAATATATTTCACAGAAAATA‑ATTTTTTAATTAACGGATTGTACAATAAGAATAAAAAAAAAAAACGCCTTATTGCGTCATGATTATgg                                             <  2:1365430/100‑1 (MQ=255)
                                                       aTCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGa                                            <  2:1704359/100‑1 (MQ=255)
                                                       aTCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGa                                            <  1:86178/100‑1 (MQ=255)
                                                         cccTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGata                                          >  2:1758952/1‑100 (MQ=255)
                                                         cccTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGata                                          >  1:2652431/1‑100 (MQ=255)
                                                          ccTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGatat                                         >  1:3307960/1‑100 (MQ=255)
                                                           cTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATc                                        >  2:2355707/1‑100 (MQ=255)
                                                             tAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGa                                      >  1:3925034/1‑100 (MQ=255)
                                                              aaCCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAc                                     >  2:433432/1‑100 (MQ=255)
                                                               aCCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAcc                                    <  1:945789/100‑1 (MQ=255)
                                                               aCCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAcc                                    <  2:1928887/100‑1 (MQ=255)
                                                               aCCAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAcc                                    <  2:2529150/100‑1 (MQ=255)
                                                                 cAATATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCACAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCAc                                  <  2:2083615/100‑1 (MQ=255)
                                                                  aaTATATTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACt                                 >  2:120170/1‑100 (MQ=255)
                                                                    tatatTTAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTc                               <  1:1859344/100‑1 (MQ=255)
                                                                       atTTAACAGAAATTA‑TTTTTTTAATTCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCaaa                            >  2:1155139/1‑100 (MQ=255)
                                                                        tttAACAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCaaaa                           >  1:216478/1‑100 (MQ=255)
                                                                           aaCAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAg                        <  1:2591450/100‑1 (MQ=255)
                                                                           aaCAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAg                        <  2:1372964/100‑1 (MQ=255)
                                                                             cAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTc                      <  2:2396542/100‑1 (MQ=255)
                                                                             cAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTc                      >  1:3285796/1‑100 (MQ=255)
                                                                             cAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTc                      >  1:2845161/1‑100 (MQ=255)
                                                                             cAGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTc                      <  1:2323740/100‑1 (MQ=255)
                                                                              aGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCa                     >  1:269700/1‑100 (MQ=255)
                                                                              aGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCa                     >  1:1886988/1‑100 (MQ=255)
                                                                              aGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCa                     <  1:667156/100‑1 (MQ=255)
                                                                              aGAAATTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCa                     <  1:2930401/100‑1 (MQ=255)
                                                                                aaaTTA‑TTTTTTTAATGCACTGACAGTTCAATCAGTATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCAATTCAAAAAAGTCAAc                   <  2:3371304/100‑1 (MQ=255)
                                                                                aaaTTA‑TTTTTTTAATGCACAGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAAc                   <  2:3027051/100‑1 (MQ=255)
                                                                                 aaTTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACg                  <  1:818779/100‑1 (MQ=255)
                                                                                 aaTTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACg                  <  1:1565140/100‑1 (MQ=255)
                                                                                 aaTTA‑TTTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACg                  <  2:376746/100‑1 (MQ=255)
                                                                                     a‑ttTTTTTAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACGacac              <  2:1297347/100‑1 (MQ=255)
                                                                                       tttttttAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACCCCCTTTTTTTTCTTTTTTATGTATATCGACCACTTCAAAAAAGTCAACGACAcc             >  2:3236617/1‑100 (MQ=255)
                                                                                         tttttAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCATATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCGACGACACCTg           <  2:3683748/100‑1 (MQ=255)
                                                                                            ttAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACGACACCTgggg        >  2:1111409/1‑100 (MQ=255)
                                                                                             tAATGCACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACGACACCTggggg       >  1:2383943/1‑100 (MQ=255)
                                                                                              aaTGCACTGAGAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTAAAAGAAGTCAACGACACCTGGGGGc      >  2:3785235/1‑100 (MQ=255)
                                                                                                  aaCTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACGACACCTGGGGGCATcc  <  2:1310267/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TTTATGTGCCATATATTCAGTGCGCTACGAGTAACAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTA‑TTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAAAAAAGTCAACGACACCTGGGGGCATCC  >  NC_000913/1093674‑1093870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A