Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,494,054 T→G S529A (TCT→GCT)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,494,0540TG92.4% 483.5 / 20.6 158S529A (TCT→GCT) trgmethyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base T (5/7);  new base G (70/75);  total (75/83)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

TCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCA  >  NC_000913/1493958‑1494150
                                                                                                |                                                                                                
tctcTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCtg                                                                                               <  1:2118868/100‑1 (MQ=255)
tctcTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCtg                                                                                               <  2:3960556/100‑1 (MQ=255)
  tctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCtgtg                                                                                             <  2:2608680/100‑1 (MQ=255)
  tctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCtgtg                                                                                             >  2:2509512/1‑100 (MQ=255)
   ctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTgtgt                                                                                            >  1:102579/1‑100 (MQ=255)
    tGGTAGAAGAGGGCTCAGAGGCGGCGGTGTTTCTTGTAGAACCGGCGGCTCGATGGACTGAGGCGGTGGATGTATGCCCTTTGGACACACGTCCTGCGTc                                                                                           <  2:582133/100‑1 (MQ=255)
    tGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTc                                                                                           <  1:3772439/100‑1 (MQ=255)
      gTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCgg                                                                                         <  1:2525313/100‑1 (MQ=255)
       tAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGc                                                                                        <  2:2692798/100‑1 (MQ=255)
       tAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGc                                                                                        <  1:2218302/100‑1 (MQ=255)
        agaagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCa                                                                                       >  1:3943915/1‑100 (MQ=255)
          aagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGa                                                                                     <  2:1335634/100‑1 (MQ=255)
           agagGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGaa                                                                                    >  1:2157491/1‑100 (MQ=255)
            gagGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAAc                                                                                   <  1:2386160/100‑1 (MQ=255)
              ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCt                                                                                 >  2:1724802/1‑100 (MQ=255)
              ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCt                                                                                 <  1:1196996/100‑1 (MQ=255)
              ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCt                                                                                 >  1:1813836/1‑100 (MQ=255)
              ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCt                                                                                 >  1:2159982/1‑100 (MQ=255)
               gCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTc                                                                                >  1:3207587/1‑100 (MQ=255)
                ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcg                                                                               >  1:3770716/1‑100 (MQ=255)
                ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcg                                                                               >  2:2335714/1‑100 (MQ=255)
                ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcg                                                                               >  2:2884054/1‑100 (MQ=255)
                 cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcgc                                                                              >  1:2977700/1‑100 (MQ=255)
                 cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcgc                                                                              <  1:2826187/100‑1 (MQ=255)
                 cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACAGAGGCGCTGGATGTATTCCGTCTGCACTAACATGCTGTGGCGGCAGAACCTcgc                                                                              <  2:3735548/100‑1 (MQ=255)
                  tcggcggcggcgGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGCGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcgcg                                                                             <  2:2952662/97‑1 (MQ=255)
                  tCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcgcg                                                                             <  1:1306832/100‑1 (MQ=255)
                  tCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTcgcg                                                                             <  1:3047026/100‑1 (MQ=255)
                   cAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCgg                                                                            <  1:3807006/100‑1 (MQ=255)
                         cggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCggg                                                                      <  1:2483273/100‑1 (MQ=255)
                         cggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCggg                                                                      >  2:3591208/1‑100 (MQ=255)
                         cggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCggg                                                                      <  1:1550837/100‑1 (MQ=255)
                         cggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCggg                                                                      <  1:314969/100‑1 (MQ=255)
                          ggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGt                                                                     <  2:2118987/100‑1 (MQ=255)
                          ggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGt                                                                     <  2:2158737/100‑1 (MQ=255)
                               tgtCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAAcc                                                                <  2:721353/100‑1 (MQ=255)
                               tgtCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAAcc                                                                <  1:3531980/100‑1 (MQ=255)
                               tggcccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAAcc                                                                <  2:3292634/97‑1 (MQ=255)
                                 tCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCag                                                              <  2:1493066/100‑1 (MQ=255)
                                 tCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCag                                                              <  2:2168756/100‑1 (MQ=255)
                                  cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagt                                                             >  1:1594040/1‑100 (MQ=255)
                                  cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagt                                                             >  2:2433082/1‑100 (MQ=255)
                                  cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCaga                                                             >  1:1519203/1‑99 (MQ=255)
                                  cccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTATGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGATGAACCagt                                                             <  2:3077591/100‑1 (MQ=255)
                                  cccTTGAAGAACAGGCGGCACGATTAACTGAAGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagt                                                             >  1:3274478/1‑100 (MQ=255)
                                   ccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtt                                                            >  1:608769/1‑100 (MQ=255)
                                   ccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtt                                                            >  2:3760493/1‑100 (MQ=255)
                                   ccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtt                                                            <  2:332735/100‑1 (MQ=255)
                                   ccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtt                                                            <  1:3857108/100‑1 (MQ=255)
                                    cTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtta                                                           >  1:1773394/1‑100 (MQ=255)
                                    cTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtta                                                           <  2:1744556/100‑1 (MQ=255)
                                    cTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagtta                                                           >  2:3454078/1‑100 (MQ=255)
                                      tGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagttagt                                                         >  1:713797/1‑100 (MQ=255)
                                       gaagaaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCagttagtt                                                        >  2:1742280/1‑100 (MQ=255)
                                         agaaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTc                                                      >  1:2669928/1‑100 (MQ=255)
                                               ggcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACg                                                >  1:1135397/1‑100 (MQ=255)
                                               ggcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACg                                                >  1:768372/1‑100 (MQ=255)
                                               ggcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACg                                                >  2:623707/1‑100 (MQ=255)
                                               ggcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACg                                                >  1:777405/1‑100 (MQ=255)
                                                gcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACgg                                               <  1:3701310/100‑1 (MQ=255)
                                                gcggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACgg                                               <  1:2335504/100‑1 (MQ=255)
                                                 cggcACGATTAACTGAGGCGGTGGATGTATTCCGTCGGCACAAACATTCTGTGTCGGCAGAACCTGGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgt                                              <  2:3989723/100‑1 (MQ=255)
                                                  ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgtg                                             >  1:103709/1‑100 (MQ=255)
                                                  ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgtg                                             <  1:1794852/100‑1 (MQ=255)
                                                  ggcACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgtg                                             <  1:2988961/100‑1 (MQ=255)
                                                     aCGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGa                                          <  2:1558108/100‑1 (MQ=255)
                                                        aTTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGaaaa                                       <  1:2447738/100‑1 (MQ=255)
                                                         ttAACTGATGCGGTGGATGTATTCCGTCTGCACAAACAGGCGGTGGCGGCAGAACCTCGCGGAGCGGGGGAACCAGTTATTGGCTCTACGGTGTGAAAAt                                      >  2:182873/1‑100 (MQ=255)
                                                          tAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATg                                     <  1:2341949/100‑1 (MQ=255)
                                                            aCTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGtt                                   >  1:1538375/1‑100 (MQ=255)
                                                             ntGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTc                                  >  2:3499687/2‑100 (MQ=255)
                                                             cTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTc                                  <  1:1990883/100‑1 (MQ=255)
                                                             cTGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTc                                  >  2:3435690/1‑100 (MQ=255)
                                                              tGAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCa                                 <  1:3036120/100‑1 (MQ=255)
                                                               gAGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCaa                                <  1:1007271/100‑1 (MQ=255)
                                                                aGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAag                               <  1:116253/100‑1 (MQ=255)
                                                                aGGCGGTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAag                               >  1:1144456/1‑100 (MQ=255)
                                                                 ggCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAagg                              >  2:1175524/1‑100 (MQ=255)
                                                                    ggTGGATGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAaggagg                           >  1:1742409/1‑100 (MQ=255)
                                                                         aTGTATTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCg                      <  2:899699/100‑1 (MQ=255)
                                                                             aTTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAg                  >  2:2958215/1‑100 (MQ=255)
                                                                             aTTCCGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAg                  >  1:1006273/1‑100 (MQ=255)
                                                                                ccGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATc               <  1:3214638/100‑1 (MQ=255)
                                                                                ccGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATc               <  2:2099270/100‑1 (MQ=255)
                                                                                ccGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATc               <  1:3501336/100‑1 (MQ=255)
                                                                                ccGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATc               <  1:440823/100‑1 (MQ=255)
                                                                                ccGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATc               >  2:1097589/1‑100 (MQ=255)
                                                                                 cGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATcc              <  2:1213389/100‑1 (MQ=255)
                                                                                 cGTCTGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATcc              >  1:2709553/1‑100 (MQ=255)
                                                                                    cTGCACACACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACAAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCtt           >  1:2557437/1‑100 (MQ=255)
                                                                                     tGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAACATGTTCAAGGAGGGATCGACAGATCCCTTc          >  1:916102/1‑100 (MQ=255)
                                                                                     tGCACAAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTc          <  1:224087/100‑1 (MQ=255)
                                                                                      ncacaAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCa         >  1:3257699/2‑100 (MQ=255)
                                                                                      gCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCa         <  2:647872/100‑1 (MQ=255)
                                                                                        acaAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCAcc       <  2:1697541/100‑1 (MQ=255)
                                                                                         caAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCt      >  1:397009/1‑100 (MQ=255)
                                                                                         caAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCt      >  2:3600967/1‑100 (MQ=255)
                                                                                         caAACATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCt      <  2:71388/100‑1 (MQ=255)
                                                                                           aaCATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCttt    >  2:1081784/1‑100 (MQ=255)
                                                                                             cATGCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCa  <  2:3221069/100‑1 (MQ=255)
                                                                                                |                                                                                                
TCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCA  >  NC_000913/1493958‑1494150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A