Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,643	T→G	I89M (ATT→ATG)	gstA →	glutathionine S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,643	0	T	G	99.2%	426.9 / ‑4.0	121	I89M (ATT→ATG)	gstA	glutathionine S‑transferase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (73/47); total (74/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 121 total aligned reads displayed.

GGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAGT  >  NC_000913/1714547‑1714737

ggATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTcc                                                                                             >  2:562770/1‑100 (MQ=255)

 gATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTccc                                                                                            <  1:2146917/100‑1 (MQ=255)

   tGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGc                                                                                          <  2:2636409/100‑1 (MQ=255)

   tGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGc                                                                                          >  2:2515774/1‑100 (MQ=255)

    gACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGcc                                                                                         >  1:3493601/1‑99 (MQ=255)

        gTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATaa                                                                                     >  1:3143055/1‑100 (MQ=255)

         tACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATaaa                                                                                    >  2:178910/1‑100 (MQ=255)

          aCTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCTGACCGCCTGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATaaaa                                                                                   <  1:3624779/100‑1 (MQ=255)

           cTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAAc                                                                                  <  1:677273/100‑1 (MQ=255)

           cTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAAc                                                                                  <  1:1395576/100‑1 (MQ=255)

            tttGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAAcc                                                                                 <  1:1848359/100‑1 (MQ=255)

             ttGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCa                                                                                >  1:2624688/1‑100 (MQ=255)

              tGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACCGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCAt                                                                               >  2:198536/1‑100 (MQ=255)

              tGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCAt                                                                               >  1:1753341/1‑100 (MQ=255)

                cTGACGGAGGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCg                                                                             <  1:3773533/100‑1 (MQ=255)

                cTGACGGAAGGCGTAGCGCGTATGCAGTGTCTTGCTGACAGCGTCGCCGACCGCCAGCTGCTGGCACGGGGGTACAGTATGTCCCGCTATAGCACCTTCg                                                                             <  2:3649737/100‑1 (MQ=255)

                cTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCg                                                                             >  1:3669199/1‑100 (MQ=255)

                cTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCg                                                                             >  1:2648609/1‑100 (MQ=255)

                 tGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGa                                                                            <  1:2305398/100‑1 (MQ=255)

                 tGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGATAAACAGTATGTCCCGCTATAAAACCATCGa                                                                            >  2:290286/1‑100 (MQ=255)

                   aCGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAAt                                                                          <  2:2930292/100‑1 (MQ=255)

                   aCGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAAt                                                                          >  1:2789857/1‑100 (MQ=255)

                     ggAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATgt                                                                        >  1:1556120/1‑99 (MQ=255)

                      gAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGc                                                                       <  2:3881407/100‑1 (MQ=255)

                      gAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGc                                                                       >  2:1821066/1‑100 (MQ=255)

                      gAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGc                                                                       <  1:3094236/100‑1 (MQ=255)

                      gAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGc                                                                       >  1:1129512/1‑100 (MQ=255)

                       aaGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCt                                                                      <  2:940803/100‑1 (MQ=255)

                       aaGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCt                                                                      >  1:2978158/1‑100 (MQ=255)

                          gCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGaa                                                                   <  2:1556227/100‑1 (MQ=255)

                           cGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAAt                                                                  >  1:1297783/1‑100 (MQ=255)

                            gTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAAtt                                                                 >  2:2536218/1‑100 (MQ=255)

                            gTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAAtt                                                                 >  2:2533668/1‑100 (MQ=255)

                            gTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAAtt                                                                 <  1:1382805/100‑1 (MQ=255)

                            gTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAAtt                                                                 >  1:1420787/1‑100 (MQ=255)

                                cGATTATGCAGTATCTTGCCGACAGCGTCCCCGCCCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAACCCATCGAATGGCTGAATTCCAt                                                             >  2:2670040/1‑100 (MQ=255)

                                cGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACAt                                                             >  2:2237224/1‑100 (MQ=255)

                                  aTTATGCAGTATCTTGCCGACAGCGTCCCCGATCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCg                                                           <  1:1021756/100‑1 (MQ=255)

                                     aTGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCa                                                        >  1:3690908/1‑100 (MQ=255)

                                             tCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTg                                                >  1:2647977/1‑100 (MQ=255)

                                             tCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTg                                                <  1:397118/100‑1 (MQ=255)

                                              cTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGc                                               <  2:2823524/100‑1 (MQ=255)

                                              cTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGc                                               >  2:2750231/1‑100 (MQ=255)

                                              cTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGc                                               >  1:3193514/1‑100 (MQ=255)

                                              cTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGc                                               >  1:2741082/1‑100 (MQ=255)

                                               ttGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCa                                              <  2:757649/100‑1 (MQ=255)

                                               ttGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCa                                              >  1:2459837/1‑100 (MQ=255)

                                                tGCCGACAGCGTCCCCGACCGCCAGTTGCTGTCACCGGTCCACACTATGTCCTGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGcgccat                                             >  2:2447837/1‑95 (MQ=255)

                                                   cGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATaaa                                          >  2:707719/1‑100 (MQ=255)

                                                     aCAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAgg                                        <  1:1336486/100‑1 (MQ=255)

                                                      cAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGATTTACATCGCCACCGAGCTGCATAAAGGt                                       <  1:3586088/100‑1 (MQ=255)

                                                         cGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTc                                    <  2:2959871/100‑1 (MQ=255)

                                                         cGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTc                                    <  1:1060255/100‑1 (MQ=255)

                                                         cGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTc                                    <  1:2823119/100‑1 (MQ=255)

                                                         cGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGAATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTc                                    <  2:3626754/100‑1 (MQ=255)

                                                          gTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTCTAAAACCATCGAATGGCTGAATTACATTGCCACCGAGCTGCATAAAGGTTTca                                   >  1:3783568/1‑100 (MQ=255)

                                                          gTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTca                                   >  1:3503380/1‑100 (MQ=255)

                                                          gTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTca                                   >  2:2869133/1‑100 (MQ=255)

                                                           tCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTcac                                  <  1:2473090/100‑1 (MQ=255)

                                                            ccccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTcaca                                 >  1:1794659/1‑100 (MQ=255)

                                                             cccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCacac                                >  1:675486/1‑100 (MQ=255)

                                                             cccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCacac                                >  1:2467836/1‑100 (MQ=255)

                                                             cccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCacac                                >  2:3917956/1‑100 (MQ=255)

                                                             cccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCacac                                >  1:119317/1‑100 (MQ=255)

                                                              ccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACAcc                               <  1:852177/100‑1 (MQ=255)

                                                              ccGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACAcc                               <  2:3094791/100‑1 (MQ=255)

                                                               cGACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACct                              >  2:1341665/1‑100 (MQ=255)

                                                                gACCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACAGctc                             <  1:3135596/100‑1 (MQ=255)

                                                                 aCCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACctct                            >  2:974670/1‑100 (MQ=255)

                                                                 aCCGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACctct                            <  1:923779/100‑1 (MQ=255)

                                                                  ccGCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTg                           <  2:1932714/100‑1 (MQ=255)

                                                                    gCCAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGtt                         >  1:1363509/1‑100 (MQ=255)

                                                                     ccAGTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGttt                        >  1:2865086/1‑100 (MQ=255)

                                                                       aGTTTCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCg                      >  2:3250368/1‑100 (MQ=255)

                                                                        gTTGCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCGGAATTAGATCGCCACGGAGCTGCACAAAGGTTTCACAACTCTTTTTCGc                     >  2:326847/1‑100 (MQ=255)

                                                                           gCTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCt                  >  2:349306/1‑100 (MQ=255)

                                                                            cTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTg                 >  2:894284/1‑100 (MQ=255)

                                                                            cTGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTg                 <  2:859613/100‑1 (MQ=255)

                                                                             tGGCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGa                <  2:3420623/100‑1 (MQ=255)

                                                                              ggCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGAt               <  1:1461916/100‑1 (MQ=255)

                                                                              ggCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGAt               >  2:1436413/1‑100 (MQ=255)

                                                                              ggCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGAt               >  2:1056659/1‑100 (MQ=255)

                                                                              ggCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGAt               >  1:1530753/1‑100 (MQ=255)

                                                                              ggCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGAt               <  1:1631313/100‑1 (MQ=255)

                                                                              ggCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGAt               >  1:2157214/1‑100 (MQ=255)

                                                                               gCACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATa              >  1:335042/1‑100 (MQ=255)

                                                                                cACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATac             >  1:3597389/1‑100 (MQ=255)

                                                                                cACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATac             >  1:3728672/1‑100 (MQ=255)

                                                                                cACCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATac             >  2:2933048/1‑100 (MQ=255)

                                                                                 aCCGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATaca            >  1:1643025/1‑100 (MQ=255)

                                                                                  ccGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATacac           >  1:2347269/1‑100 (MQ=255)

                                                                                  ccGGTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATacac           >  1:3925829/1‑100 (MQ=255)

                                                                                    ggTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCg         >  1:2274334/1‑100 (MQ=255)

                                                                                    ggTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCg         >  2:444439/1‑100 (MQ=255)

                                                                                    ggTAAACAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCg         >  1:3731413/1‑100 (MQ=255)

                                                                                    ggTAAACAGTAAGTCCCGCTATAAAACCATCGAATGGCTGAATTACCTCGCCACCGAGCTGCATAAAGGTTTCACACATCCGTTTCGCCCTGATACACCg         >  2:1795732/1‑100 (MQ=255)

                                                                                       aaaCAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGaa      <  2:762870/100‑1 (MQ=255)

                                                                                        aaCAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGAag     <  1:453041/100‑1 (MQ=255)

                                                                                          cAGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGAagag   <  2:395221/100‑1 (MQ=255)

                                                                                           aGTATGTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAGt  >  1:39005/1‑100 (MQ=255)

GGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCACACCTCTGTTTCGCCCTGATACACCGGAAGAGT  >  NC_000913/1714547‑1714737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A