Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,851	T→G	L279R (CTA→CGA)	mdtA →	multidrug efflux system, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,851	0	T	G	87.8%	432.9 / 48.4	165	L279R (CTA→CGA)	mdtA	multidrug efflux system, subunit A
Reads supporting (aligned to +/- strand): ref base T (13/7); new base G (70/74); total (83/82)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

Only 100 of 167 total aligned reads displayed.

CCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACG  >  NC_000913/2154755‑2154946

 cGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCAGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATAAAGTGAAGGCACGCTGTTAAGTAGAGAt                                                                                             >  2:3296650/1‑100 (MQ=255)

  gTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATa                                                                                            <  2:36030/100‑1 (MQ=255)

    aGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGGACCAACTCGAAGAAATTAAGGGAAGGCACGCTGTTAAGTCGAGATAAc                                                                                          <  2:3976044/100‑1 (MQ=255)

    aGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAc                                                                                          <  2:646984/100‑1 (MQ=255)

    aGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAc                                                                                          >  2:3664699/1‑100 (MQ=255)

    aGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAc                                                                                          <  1:1366120/100‑1 (MQ=255)

    aGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGCATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAc                                                                                          <  1:757214/100‑1 (MQ=255)

     gTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGACGAGATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAcc                                                                                         >  1:1282141/1‑100 (MQ=255)

     gTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAcc                                                                                         >  1:1793369/1‑100 (MQ=255)

     gTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAAcc                                                                                         >  1:1179939/1‑100 (MQ=255)

       gCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCaa                                                                                       >  2:1105359/1‑100 (MQ=255)

        cAGGCGCAGAAAGCCGGAAAACCACGGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTTAAGGCACGCTGTTAAGTCGAGATAACCaaa                                                                                      >  1:3888000/1‑100 (MQ=255)

          ggCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATc                                                                                    <  2:2809200/100‑1 (MQ=255)

            cgcAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGa                                                                                  <  1:1086965/100‑1 (MQ=255)

             gcAGAAAGCCGGAAAACCGCTGGGGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGAt                                                                                 <  2:320940/100‑1 (MQ=255)

              cAGGAAGACGGAAAACCGAAGGTGGGAGGAGCCTGGGATGGGACAAAATGGAAAAAATTGAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATg                                                                                <  2:3288171/100‑1 (MQ=255)

              cAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATg                                                                                <  2:1686600/100‑1 (MQ=255)

              cAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATg                                                                                <  1:3444096/100‑1 (MQ=255)

               aGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGc                                                                               <  2:1995494/100‑1 (MQ=255)

                gAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGcc                                                                              <  1:694244/100‑1 (MQ=255)

                gAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAAGCACGCTGTTAAGTCGAGATAACCAAATCGATGcc                                                                              >  2:3230651/1‑100 (MQ=255)

                 aaaGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCa                                                                             >  1:3293744/1‑100 (MQ=255)

                  aaGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCAc                                                                            >  1:2261686/1‑100 (MQ=255)

                   aGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGACACt                                                                           <  2:3381069/100‑1 (MQ=255)

                    gCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTa                                                                          >  1:4068792/1‑100 (MQ=255)

                     ccGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCACCTCGAAGAAATTAAGTGAAGGCACGCCGTTAAGTCTAGATAACCAAATCGATGCCACTAc                                                                         >  2:3039647/1‑100 (MQ=255)

                      cGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTAcc                                                                        <  2:3240798/100‑1 (MQ=255)

                       ggAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCg                                                                       >  1:2230753/1‑100 (MQ=255)

                       ggAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCg                                                                       <  1:3643842/100‑1 (MQ=255)

                        gAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCgg                                                                      >  1:2431443/1‑100 (MQ=255)

                         aaaaCCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGt                                                                     >  1:1854799/1‑100 (MQ=255)

                         aaaaCCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGt                                                                     <  1:2925150/100‑1 (MQ=255)

                           aaCCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTAc                                                                   >  2:2152318/1‑100 (MQ=255)

                            aCCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACg                                                                  <  2:1731587/100‑1 (MQ=255)

                             ccGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGa                                                                 >  2:3522972/1‑100 (MQ=255)

                              cGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGAt                                                                >  1:1923307/1‑100 (MQ=255)

                              cGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGAt                                                                <  1:3362812/100‑1 (MQ=255)

                              cGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGAt                                                                <  1:2771601/100‑1 (MQ=255)

                              cGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGACACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGAt                                                                >  1:2896325/1‑100 (MQ=255)

                                cTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTa                                                              <  1:704537/100‑1 (MQ=255)

                                  ggtggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTaaa                                                            >  1:2297534/1‑100 (MQ=255)

                                  ggtggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTaaa                                                            >  1:626589/1‑100 (MQ=255)

                                  ggtggtAGAAGCCTGGGATCGCACCAACGCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTaaa                                                            <  1:213292/100‑1 (MQ=255)

                                   gtggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAg                                                           <  1:978220/100‑1 (MQ=255)

                                   gtggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAg                                                           <  2:2293384/100‑1 (MQ=255)

                                   gtggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAg                                                           <  2:2934041/100‑1 (MQ=255)

                                   gtggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAg                                                           >  1:1839730/1‑100 (MQ=255)

                                   ctggtAAAATCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATTACCAAATCGATGCCACTACCGGTACGATTAAAg                                                           <  2:3316140/99‑1 (MQ=255)

                                     ggtAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTg                                                         >  1:3449395/1‑100 (MQ=255)

                                     ggtAGAAGCCTGAGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTg                                                         <  2:586522/100‑1 (MQ=255)

                                       tAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGaa                                                       >  2:1700616/1‑100 (MQ=255)

                                            gCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCAc                                                  >  1:2190872/1‑100 (MQ=255)

                                                gggATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCtt                                              >  1:1463028/1‑100 (MQ=255)

                                                 ggATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCttt                                             <  1:1606501/100‑1 (MQ=255)

                                                 ggATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCttt                                             >  2:1238619/1‑100 (MQ=255)

                                                  gATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACCACCGGTACGATTAAAGTGAAAGCACGCTTta                                            >  1:3554039/1‑100 (MQ=255)

                                                    tCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTtaat                                          <  2:1093666/100‑1 (MQ=255)

                                                      gCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTtaataa                                        >  2:3351720/1‑100 (MQ=255)

                                                      gCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTtaataa                                        >  2:1443195/1‑100 (MQ=255)

                                                      gCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCGTtaataa                                        >  2:746590/1‑100 (MQ=255)

                                                        aCCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATc                                      <  1:1257120/100‑1 (MQ=255)

                                                         ccAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCa                                     <  2:969244/100‑1 (MQ=255)

                                                          cAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAg                                    >  1:2479470/1‑100 (MQ=255)

                                                          cAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAg                                    <  2:2521809/100‑1 (MQ=255)

                                                           aaCTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAgg                                   >  1:2398582/1‑100 (MQ=255)

                                                             cTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgat                                 >  2:2346584/1‑100 (MQ=255)

                                                             cTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgat                                 >  2:467603/1‑100 (MQ=255)

                                                              tCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatg                                >  1:1507057/1‑100 (MQ=255)

                                                              tCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatg                                <  2:2893983/100‑1 (MQ=255)

                                                               cGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatga                               >  2:1047490/1‑100 (MQ=255)

                                                                gaagaaATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatgat                              >  1:1994038/1‑100 (MQ=255)

                                                                gaagaaATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGgatgat                              >  2:2314909/1‑100 (MQ=255)

                                                                 aagaaATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGatgatg                             >  1:2339141/1‑100 (MQ=255)

                                                                 aagaaATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGatgatg                             >  1:394019/1‑100 (MQ=255)

                                                                  agaaATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATgc                            >  2:3159451/1‑100 (MQ=255)

                                                                  agaaATTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTCAAAGCACGCTCTAATAATAAGGATGATgc                            >  2:2151594/1‑100 (MQ=255)

                                                                    aaaTTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATgcgc                          >  1:543488/1‑100 (MQ=255)

                                                                     aaTTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCt                         >  2:72760/1‑100 (MQ=255)

                                                                      aTTAAGTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTg                        <  1:3330571/100‑1 (MQ=255)

                                                                          aGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTc                    >  1:2364146/1‑100 (MQ=255)

                                                                           gTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTcc                   <  2:549443/100‑1 (MQ=255)

                                                                           gTGAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTcc                   <  2:2574414/100‑1 (MQ=255)

                                                                             gAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCa                 <  1:1069366/100‑1 (MQ=255)

                                                                             gAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCa                 >  2:2100159/1‑100 (MQ=255)

                                                                             gAAGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCa                 <  2:1447271/100‑1 (MQ=255)

                                                                              aaGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCaa                <  2:2494523/100‑1 (MQ=255)

                                                                              aaGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCaa                <  2:1962713/100‑1 (MQ=255)

                                                                              aaGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCaa                <  2:1157209/100‑1 (MQ=255)

                                                                               aGGCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAAt               >  2:2769749/1‑100 (MQ=255)

                                                                                ggCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATc              >  1:2326658/1‑100 (MQ=255)

                                                                                ggCACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATc              <  1:1717071/100‑1 (MQ=255)

                                                                                  cACGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAg            <  1:965149/100‑1 (MQ=255)

                                                                                   aCGCTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGt           >  2:831695/1‑100 (MQ=255)

                                                                                      cTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCATTCAGTTTg        <  2:3830138/100‑1 (MQ=255)

                                                                                      cTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTg        <  1:3850419/100‑1 (MQ=255)

                                                                                      cTGTTAAGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTg        <  2:1513383/100‑1 (MQ=255)

                                                                                         ttAAGTCGAGATAATCAAATCGATGCCACTACCGGTACGATGAAAGTGAAAGCATGCTTTAATACTCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTa     <  2:3907567/100‑1 (MQ=255)

                                                                                           aaGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGATAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTatc   >  2:1193504/1‑98 (MQ=255)

                                                                                            aGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAAcg  <  2:2394121/100‑1 (MQ=255)

                                                                                            aGTCGAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAAcg  >  2:1207064/1‑100 (MQ=255)

CCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACG  >  NC_000913/2154755‑2154946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A