Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,361,733	A→C	L160R (CTG→CGG)	rhmR ←	putative DNA‑binding transcriptional regulator for the rhm operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,733	0	A	C	96.9%	430.3 / 1.6	129	L160R (CTG→CGG)	rhmR	putative DNA‑binding transcriptional regulator for the rhm operon
Reads supporting (aligned to +/- strand): ref base A (2/2); new base C (60/64); total (63/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 129 total aligned reads displayed.

CATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCAGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATG  >  NC_000913/2361641‑2361830

cATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGGCCCGCCCTTc                                                                                            <  2:3679324/100‑1 (MQ=255)

cATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTc                                                                                            <  2:3840573/100‑1 (MQ=255)

cATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTc                                                                                            <  1:2741807/100‑1 (MQ=255)

cATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTc                                                                                            <  2:3041798/100‑1 (MQ=255)

cATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCAGCCCTTc                                                                                            >  2:2157650/1‑100 (MQ=255)

 ntAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAGTCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCg                                                                                           >  1:1077395/2‑100 (MQ=255)

 aTAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCg                                                                                           >  2:10040/1‑100 (MQ=255)

  tAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGa                                                                                          <  1:2401172/100‑1 (MQ=255)

   aGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCTCCTGCTCCCAGACCCGCCCTTCGat                                                                                         <  1:304324/100‑1 (MQ=255)

   aGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGat                                                                                         <  1:2400644/100‑1 (MQ=255)

   aGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGat                                                                                         <  1:3494288/100‑1 (MQ=255)

   aGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGat                                                                                         <  2:1752802/100‑1 (MQ=255)

   aGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGat                                                                                         <  2:654272/100‑1 (MQ=255)

    gCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGata                                                                                        >  1:1928687/1‑100 (MQ=255)

    gCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGata                                                                                        >  2:3253659/1‑100 (MQ=255)

      tCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGataat                                                                                      >  2:3143939/1‑100 (MQ=255)

        cAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGTATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATAc                                                                                    <  1:556429/100‑1 (MQ=255)

        cAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATAc                                                                                    <  2:2112394/100‑1 (MQ=255)

          gCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCAGCCCTTCGATAATACtt                                                                                  <  1:220225/100‑1 (MQ=255)

           cccTCACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACttt                                                                                 <  1:3659167/100‑1 (MQ=255)

            ccTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCTGCCCTTCGATAATACTTTg                                                                                >  1:2696979/1‑100 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               <  1:1616168/100‑1 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               <  2:3239478/100‑1 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               <  2:2430715/100‑1 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               <  1:678888/100‑1 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               >  1:1324785/1‑100 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               >  1:1388499/1‑100 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               <  2:1363813/100‑1 (MQ=255)

             cTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGc                                                                               >  1:2499639/1‑100 (MQ=255)

              tGACGGCGAATACGCGCCAGTTCTTCTTGAAGTTGCTGTGGATGAGTAATTGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCt                                                                              >  1:2765017/1‑100 (MQ=255)

               gACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTg                                                                             <  1:241423/100‑1 (MQ=255)

                   gatcttACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTAcc                                                                         <  2:342220/95‑1 (MQ=255)

                   gCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTAcc                                                                         >  2:1822519/1‑100 (MQ=255)

                   gCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTAcc                                                                         <  1:2089509/100‑1 (MQ=255)

                    cGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCg                                                                        <  1:1907003/100‑1 (MQ=255)

                       aTACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCGGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTg                                                                     <  1:3474269/100‑1 (MQ=255)

                        tACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGc                                                                    >  2:948393/1‑100 (MQ=255)

                          cgcgCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTTCTGTACCGCTGCAg                                                                  >  1:3883824/1‑100 (MQ=255)

                          cgcgCCAGTTCTTCGAGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAg                                                                  >  2:3893319/1‑100 (MQ=255)

                            cgcCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGt                                                                >  2:3742155/1‑100 (MQ=255)

                                aGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGcc                                                            >  1:410044/1‑100 (MQ=255)

                                  ttcttcGTGTAGTTGTTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGTCCTTCGATAATTCTTTGCTGTACCGCTGCAGGTTGCCAg                                                          <  1:1789602/100‑1 (MQ=255)

                                   tcttcGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCCTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAgg                                                         <  2:847274/100‑1 (MQ=255)

                                    cttcGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGc                                                        >  2:2981841/1‑100 (MQ=255)

                                    cttcATGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGc                                                        >  1:9066/1‑100 (MQ=255)

                                        gTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGc                                                    <  1:261386/100‑1 (MQ=255)

                                        gTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGc                                                    >  1:196777/1‑100 (MQ=255)

                                        gTGAAGTTGCTGTGGATGAGTAATCGTTGTGAGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGc                                                    >  1:637779/1‑100 (MQ=255)

                                         tGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCa                                                   <  1:60361/100‑1 (MQ=255)

                                            aGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGGCCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGc                                                <  2:3751449/100‑1 (MQ=255)

                                            aGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGc                                                >  2:532896/1‑100 (MQ=255)

                                             gTTGCTGTGGCTGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACTCGCCCTTAGATAATACTTTGCTGTACCGCTGCCGGTTGCTAGGCAAGCAGGCa                                               >  2:2358016/1‑100 (MQ=255)

                                             gTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCa                                               >  2:346941/1‑100 (MQ=255)

                                                gCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCAttt                                            >  2:1532020/1‑100 (MQ=255)

                                                gCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCAttt                                            >  1:2316193/1‑100 (MQ=255)

                                                  tgtgGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTAc                                          >  2:2186685/1‑100 (MQ=255)

                                                  tgtgGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTG‑CAGGCAAGCAGGCATTTAcc                                         <  2:3236116/100‑1 (MQ=255)

                                                   gtgGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTAcc                                         >  2:1124422/1‑100 (MQ=255)

                                                   gtgGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTAcc                                         <  1:3989037/100‑1 (MQ=255)

                                                    tgGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCt                                        >  1:1839814/1‑100 (MQ=255)

                                                         gAGTAACCGTTGTGAGTACCGCCAACTCCCAGACCCGCCCTCCCATAATACTTTGCTGTACCGCTGCAGGATACAAGCCAAGCAGGCATTTACCTATTcc                                   <  2:3802500/100‑1 (MQ=255)

                                                             aaTCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAg                               <  1:2228020/100‑1 (MQ=255)

                                                             aaTCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAg                               <  2:1677746/100‑1 (MQ=255)

                                                             aaTCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAg                               <  1:50543/100‑1 (MQ=255)

                                                             aaTCGTTGTGGGAGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAg                               >  1:3518240/1‑100 (MQ=255)

                                                              aTCGTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGc                              <  2:3547743/100‑1 (MQ=255)

                                                                 gTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGAt                           >  1:2508379/1‑100 (MQ=255)

                                                                 gTTGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGAt                           <  2:1941791/100‑1 (MQ=255)

                                                                 gTTGTGAGTGTCGCCTGCCCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCCAt                           >  1:3407792/1‑100 (MQ=255)

                                                                  ttGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATa                          <  1:341989/100‑1 (MQ=255)

                                                                  ttGTGGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATa                          <  1:721485/100‑1 (MQ=255)

                                                                  ttGTGGGTGTCACCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATa                          <  1:2872460/100‑1 (MQ=255)

                                                                   tgtgGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATaa                         <  1:1406805/100‑1 (MQ=255)

                                                                   tgtgGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATaa                         >  1:3852389/1‑100 (MQ=255)

                                                                    gtgGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATaaa                        <  2:295858/100‑1 (MQ=255)

                                                                     tgGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAg                       <  2:838913/100‑1 (MQ=255)

                                                                     tgGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAg                       >  1:427705/1‑100 (MQ=255)

                                                                     tgGGTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAg                       <  2:686416/100‑1 (MQ=255)

                                                                       ggTGTCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCg                     >  1:1969293/1‑100 (MQ=255)

                                                                          gtCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGaaa                  <  1:2995653/100‑1 (MQ=255)

                                                                          gtCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGaaa                  >  2:2527450/1‑100 (MQ=255)

                                                                           tCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGaaan                 <  1:3192038/100‑2 (MQ=255)

                                                                           tCGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAg                 <  1:1248466/100‑1 (MQ=255)

                                                                            cGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGCGCGATAAAGCGAAAgg                >  2:3572436/1‑100 (MQ=255)

                                                                            cGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAgg                >  2:1325799/1‑100 (MQ=255)

                                                                            cGCCTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAgg                >  2:2717258/1‑100 (MQ=255)

                                                                               cTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCtt             <  1:3788235/100‑1 (MQ=255)

                                                                               cTGCTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCtt             >  1:252509/1‑100 (MQ=255)

                                                                                  cTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCttttt          >  1:1361920/1‑100 (MQ=255)

                                                                                  cTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCttttt          <  2:662480/100‑1 (MQ=255)

                                                                                  cTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCttttt          >  2:1963592/1‑100 (MQ=255)

                                                                                  cTCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCttttt          >  1:3566076/1‑100 (MQ=255)

                                                                                   tCCCAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTc         <  2:3256371/100‑1 (MQ=255)

                                                                                    cccAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTcc        <  2:1507373/100‑1 (MQ=255)

                                                                                     ccAGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCt       >  2:1155573/1‑100 (MQ=255)

                                                                                      naGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCtt      >  1:1194544/2‑100 (MQ=255)

                                                                                       aGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTc     >  2:3908615/1‑100 (MQ=255)

                                                                                       aGACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTc     <  2:429591/100‑1 (MQ=255)

                                                                                        gACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCa    <  1:543706/100‑1 (MQ=255)

                                                                                        gACCCGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCa    <  2:1339288/100‑1 (MQ=255)

CATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTCTTCGTGAAGTTGCTGTGGATGAGTAATCGTTGTGGGTGTCGCCTGCTCCCAGACCAGCCCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATG  >  NC_000913/2361641‑2361830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A