Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,397,296 A→C P49P (CCT→CCG)  nuoH ← NADH:ubiquinone oxidoreductase, membrane subunit H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,397,2960AC92.8% 446.8 / 18.9 152P49P (CCT→CCG) nuoHNADH:ubiquinone oxidoreductase, membrane subunit H
Reads supporting (aligned to +/- strand):  ref base A (5/6);  new base C (65/76);  total (70/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 152 total aligned reads displayed.

TGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGC  >  NC_000913/2397199‑2397391
                                                                                                 |                                                                                               
tGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCgg                                                                                               <  1:1125375/100‑1 (MQ=255)
tGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCgg                                                                                               <  2:2304128/100‑1 (MQ=255)
tGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCgg                                                                                               <  2:3184630/100‑1 (MQ=255)
tGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCgg                                                                                               <  1:1988167/100‑1 (MQ=255)
  aCGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTc                                                                                             <  2:3968086/100‑1 (MQ=255)
    gcgATTCGAGAATTTCGGGATCTATTCTTCTGTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCTCTAGCCAACACGATTCGGTCCg                                                                                           <  2:395412/100‑1 (MQ=255)
     ggATCCGAGAGGGGCGGGATGCAGTCTTCTTTAAAGAGCATTTTGATGATGTCGGCAGCCAGCTGGGGAGAACCGCCCAAGCCAACACGGTTCGGTCCGt                                                                                          <  2:1398723/99‑1 (MQ=255)
     cgATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGt                                                                                          >  1:2569749/1‑100 (MQ=255)
      gATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTa                                                                                         >  2:3072565/1‑100 (MQ=255)
      gATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTa                                                                                         <  2:369400/100‑1 (MQ=255)
       aTCCGAGAATTTCGGGATCCAATCTTCTTTAAATAACATTTTGGACATGTCCGTAACCTGCTGTAAAGAACCGCCCCAGCCAGCACGGTTCGGTCCggta                                                                                        >  2:3565442/1‑97 (MQ=255)
          cGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACgg                                                                                     >  2:815851/1‑100 (MQ=255)
           gagaATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGt                                                                                    >  1:2683742/1‑100 (MQ=255)
            agaATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGtt                                                                                   >  1:3120062/1‑100 (MQ=255)
             gaATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTc                                                                                  <  1:433385/100‑1 (MQ=255)
             gaATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTc                                                                                  >  1:2340911/1‑100 (MQ=255)
              aaTTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCt                                                                                 <  2:1820588/100‑1 (MQ=255)
               aTTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTg                                                                                >  2:291256/1‑100 (MQ=255)
               aTTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTg                                                                                <  1:4007908/100‑1 (MQ=255)
                tttCGGGATCCCGTCTTCTTTAAAGAACATTTTGATCATGCCCGCAACCAGCTGGAGCGAACCGCCCCAGCTTACACGGTTCGGTCAGTAACGGTTCTgg                                                                               <  1:3597962/100‑1 (MQ=255)
                tttCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCTAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTgg                                                                               <  1:3036066/100‑1 (MQ=255)
                 ttCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGa                                                                              <  1:1739934/100‑1 (MQ=255)
                  tCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGaa                                                                             <  1:574338/100‑1 (MQ=255)
                   cGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAAc                                                                            <  1:2145275/100‑1 (MQ=255)
                   cGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAAc                                                                            <  2:2444497/100‑1 (MQ=255)
                   cGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAAc                                                                            <  1:3382140/100‑1 (MQ=255)
                   cGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAAc                                                                            <  1:2780689/100‑1 (MQ=255)
                    gggATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACa                                                                           <  2:3757752/100‑1 (MQ=255)
                     ggATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAg                                                                          >  1:3925410/1‑100 (MQ=255)
                       aTCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGAc                                                                        >  2:2802144/1‑100 (MQ=255)
                       aTCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGAc                                                                        >  2:517018/1‑100 (MQ=255)
                       aTCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGAc                                                                        <  1:2057063/100‑1 (MQ=255)
                        tCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGAcc                                                                       >  1:3910076/1‑100 (MQ=255)
                         ccAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGAccc                                                                      <  2:250815/100‑1 (MQ=255)
                          cAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCca                                                                     >  2:1633263/1‑100 (MQ=255)
                          cAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCca                                                                     <  1:3492831/100‑1 (MQ=255)
                           aGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCcag                                                                    >  1:550715/1‑100 (MQ=255)
                           aGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCcag                                                                    >  1:3921300/1‑100 (MQ=255)
                            gTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCcagc                                                                   <  1:2990183/100‑1 (MQ=255)
                                tcttTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGc                                                               <  2:3652539/100‑1 (MQ=255)
                                 gtttAAAGAACATGTTGATCATGTCCGTAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCg                                                              <  1:1974405/99‑1 (MQ=255)
                                 cttTGAAGAACATTTTGATCATGTCCGGAACAAGCTGGAGCGAACGGCCCCAGCCAACACTGTTCGGTCCGTAACGGATCTGGAACAGACCGAGCAGTCg                                                              >  2:3395237/1‑100 (MQ=255)
                                 cttTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCg                                                              >  1:3699768/1‑100 (MQ=255)
                                  tttAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGa                                                             >  2:2185289/1‑100 (MQ=255)
                                   ttAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGAc                                                            <  1:3446360/100‑1 (MQ=255)
                                   ttAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGATCTGGAACAGACCGAGCAGGCGAc                                                            >  2:1030884/1‑100 (MQ=255)
                                   ttAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGAc                                                            <  2:569549/100‑1 (MQ=255)
                                    tAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACg                                                           <  1:692735/100‑1 (MQ=255)
                                      aaGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGtt                                                         >  2:2846866/1‑100 (MQ=255)
                                        gAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCg                                                       <  1:3526058/100‑1 (MQ=255)
                                          aCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGcc                                                     >  1:365117/1‑100 (MQ=255)
                                           cATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                    >  1:4075111/1‑100 (MQ=255)
                                           cATTTTGATCATGCCCGCAACCAGCGAGGGCGAAGCGCACCAGCGAGCACGGTTCGGTCGGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                    <  2:958827/100‑1 (MQ=255)
                                            aTTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCaa                                                   >  1:2471329/1‑100 (MQ=255)
                                            aTTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCaa                                                   >  1:1354352/1‑100 (MQ=255)
                                             ttttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTGTGGAACAGACCCAGCAGGCGACGTTCGCCaaa                                                  >  2:4003059/1‑100 (MQ=255)
                                             ttttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGCCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCaaa                                                  >  2:710016/1‑100 (MQ=255)
                                               ttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGc                                                <  2:1164377/100‑1 (MQ=255)
                                               ttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGc                                                <  2:3590351/100‑1 (MQ=255)
                                               ttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGc                                                <  2:3868732/100‑1 (MQ=255)
                                                tGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCt                                               >  2:2981010/1‑100 (MQ=255)
                                                tGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCt                                               >  1:1150395/1‑100 (MQ=255)
                                                  aTCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGACCGTAACGGTTCTGGAACAGACCCAGAAGGCGACGTTCGCCAAAGCCCa                                             >  2:1795892/1‑100 (MQ=255)
                                                    cATGTCCGCAACCAGCTGGAGCGACCCGCCCCACCCAACACGGTTCGATCCGTAACGGTTCTGAAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg                                           <  1:2317037/100‑1 (MQ=255)
                                                    cATGTCCGCAACCAGCTGGAGCGAACCGCCCCCGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACGCAGCAGGCGACGTTCGCCAAAGCTCATg                                           >  2:2541865/1‑100 (MQ=255)
                                                    cATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg                                           <  2:2081886/100‑1 (MQ=255)
                                                    cATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg                                           <  1:3722314/100‑1 (MQ=255)
                                                    cATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg                                           <  1:2890333/100‑1 (MQ=255)
                                                       gTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAAt                                        <  1:2321933/100‑1 (MQ=255)
                                                       gTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAAt                                        <  2:1853591/100‑1 (MQ=255)
                                                       gTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAAt                                        >  1:3574395/1‑100 (MQ=255)
                                                         ccGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGc                                      >  2:2210239/1‑100 (MQ=255)
                                                         ccGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTATGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGACTGc                                      >  2:1592207/1‑100 (MQ=255)
                                                          cGCAACCAGCTGGAGCGAACCGCCCAAGCCAACACGGTTCGGTCGGTAACGGTTCTGGAACAGACCCAGCAAGCGACGTTCGCCAAAGCTCATGAATGcc                                     >  2:424358/1‑100 (MQ=255)
                                                            cAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGcccc                                   <  1:803634/100‑1 (MQ=255)
                                                              aCCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGc                                 >  2:3794037/1‑100 (MQ=255)
                                                                  gCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGt                             >  1:3403792/1‑100 (MQ=255)
                                                                   cTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTg                            <  1:647131/100‑1 (MQ=255)
                                                                   cTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTg                            <  1:608810/100‑1 (MQ=255)
                                                                     ggAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGag                          >  2:633686/1‑99 (MQ=255)
                                                                     ggAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGAc                          <  1:1351251/100‑1 (MQ=255)
                                                                       aGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACaa                        >  2:879814/1‑100 (MQ=255)
                                                                          gAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAacca                     >  1:3915754/1‑100 (MQ=255)
                                                                          gAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAacca                     >  2:236358/1‑100 (MQ=255)
                                                                            aCCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAaccacc                   <  2:3834032/100‑1 (MQ=255)
                                                                              cGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcag                 >  2:3381727/1‑100 (MQ=255)
                                                                               gCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc                >  1:1623372/1‑100 (MQ=255)
                                                                               gCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc                >  2:2423109/1‑100 (MQ=255)
                                                                                 cgcAGCCAACACGGTGCGGTACGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagcag              <  2:3142594/98‑1 (MQ=255)
                                                                                 cccAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagcag              >  2:1014874/1‑100 (MQ=255)
                                                                                   cAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGa            >  2:2043953/1‑100 (MQ=255)
                                                                                   cAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGa            <  1:2244680/100‑1 (MQ=255)
                                                                                     gCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATc          <  1:2602264/100‑1 (MQ=255)
                                                                                     gCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATc          >  1:68246/1‑100 (MQ=255)
                                                                                       cAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcac        >  1:2936847/1‑100 (MQ=255)
                                                                                        aaCACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacc       >  2:1146796/1‑100 (MQ=255)
                                                                                         acacGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca      >  2:1785755/1‑100 (MQ=255)
                                                                                           acGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCaccacc    <  2:1371362/100‑1 (MQ=255)
                                                                                           acGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCaccacc    <  1:1542777/100‑1 (MQ=255)
                                                                                            cGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCg   <  1:3944737/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGC  >  NC_000913/2397199‑2397391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A