Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I0 R1	76	110.5	2404748	96.5%	2320581	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	19,294	C→T	48.6%	T317I (ACT→ATT)	gdpP →	Cyclic‑di‑AMP phosphodiesterase GdpP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	19,294	0	C	T	48.6%	0.9 / 163.9	122	T317I (ACT→ATT)	gdpP	Cyclic‑di‑AMP phosphodiesterase GdpP
Reads supporting (aligned to +/- strand): ref base C (36/26); new base T (32/26); total (70/52)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 123 total aligned reads displayed.

AAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGCAATGATG  >  USA300TCH1516_ALE/19154‑19433

aaaTTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTAc                                                                                                                                             <  1:172787/141‑1 (MQ=255)

    ttAATCGTGTTAGGTGAATTATGACAATCAGGCCTAGACTTAGCAGTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTGAGACTGACCCGATGGAGAAACGTACTCgt                                                                                                                                         <  2:582141/141‑1 (MQ=255)

      aaTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAAGGTGCGTTTCTATGACGGTAAGCCTGACCCGAGGGAGAAAGGTACTctggg                                                                                                                                       >  2:1114597/1‑137 (MQ=255)

      aaTCGACTTAGGTGAATTATCAAAATCAGGCCTAAACTTAGCATTAGGACGCGGGGGCGAACAAGTTGCAATTAAAAGTATTAGTGGTAATGTGCGTTTCTATGACGGTAGAAATGACTCGATGTAGTAGCGTCATCgtga                                                                                                                                       >  2:707455/1‑140 (MQ=255)

       aTCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTa                                                                                                                                      <  1:717083/141‑1 (MQ=255)

         cGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTTTAag                                                                                                                                    >  2:830993/1‑141 (MQ=255)

           aCTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAagag                                                                                                                                  <  1:972345/141‑1 (MQ=255)

           aCTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAagag                                                                                                                                  >  2:613108/1‑141 (MQ=255)

             ttAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGAAGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAGACGTACTCGTGTAAGAGCa                                                                                                                                >  2:233040/1‑141 (MQ=255)

               aGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAGTGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAAAGTCCTCGTGTAAGGACACg                                                                                                                              >  2:457510/1‑141 (MQ=255)

               aGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACg                                                                                                                              >  1:21115/1‑141 (MQ=255)

                   gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACgtggg                                                                                                                          >  1:1080122/1‑139 (MQ=255)

                   gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGtgtg                                                                                                                          <  1:981943/141‑1 (MQ=255)

                   gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTGAAAGCACGtttg                                                                                                                          >  2:418531/1‑141 (MQ=255)

                   gAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGtgtg                                                                                                                          >  2:433614/1‑141 (MQ=255)

                        aTCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGAtctc                                                                                                                     >  2:900485/1‑141 (MQ=255)

                         tCACAATCAGGCCTAGACTTAGCATTAGGACGCGTAGGCGACGAATTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTca                                                                                                                    <  2:419793/141‑1 (MQ=255)

                          cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAACAGTCTTAGTGGTAATGTGCGTTTCTCTGACGGTAAGACTGGCCCGACGGAGAAACGTAGTCGTGCGTGAGCAGGTGTGaggtcgc                                                                                                                   >  1:13455/1‑135 (MQ=255)

                          cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTcac                                                                                                                   >  1:1035126/1‑141 (MQ=255)

                          cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTcac                                                                                                                   >  1:478527/1‑141 (MQ=255)

                          cacaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTcac                                                                                                                   >  2:1173267/1‑141 (MQ=255)

                           acaATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGGGTAAGAGCACGTGTGATCTcaca                                                                                                                  >  2:673864/1‑141 (MQ=255)

                            caATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                                                 <  2:280622/141‑1 (MQ=255)

                            caATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                                                 <  1:542601/141‑1 (MQ=255)

                             aaTCAGGCCCAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATg                                                                                                                <  1:757372/141‑1 (MQ=255)

                               tCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCGATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                                                 >  2:652851/1‑138 (MQ=255)

                               tCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAt                                                                                                                 <  1:652851/138‑1 (MQ=255)

                                 aGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGtt                                                                                                            >  1:782241/1‑141 (MQ=255)

                                   gacTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                                                          >  2:141301/3‑141 (MQ=255)

                                   gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                                                          >  1:708139/1‑141 (MQ=255)

                                   gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                                                          <  1:226907/141‑1 (MQ=255)

                                   gCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTaa                                                                                                          >  1:1196721/1‑141 (MQ=255)

                                        gACTGAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGat                                                                                                     <  1:526682/141‑1 (MQ=255)

                                            tAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATcc                                                                                                 >  2:1184972/1‑141 (MQ=255)

                                             aGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCt                                                                                                <  1:141301/141‑1 (MQ=255)

                                              gCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGAGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCCCGTGTGATCTCACATGCGTCAAAAGATATCCtt                                                                                               >  2:271367/1‑141 (MQ=255)

                                               cATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTg                                                                                              <  1:900485/141‑1 (MQ=255)

                                                 ttAGGACGCGTGGGCGACCAAGTTGCAATTAAAAGTATTAAAGGTAATGTGCGTTTCTATGGCCGTAAGACTGACCCGATGTAGAACCGTCCTCGTGTAAGAGCACGTGTGATCTCAAATGCTTTAAAAGATCTGCTTgta                                                                                            <  2:635146/141‑3 (MQ=255)

                                                 ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCa                                                                                            >  1:952935/1‑141 (MQ=255)

                                                 ttAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCa                                                                                            >  1:185129/1‑141 (MQ=255)

                                                    ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCcgag                                                                                         >  1:266266/1‑141 (MQ=255)

                                                    ggACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTGTCAAAGGGGGTAAGAGTGTCCCGATGGTGACACGTATTCTTGTGAGAGCACGTGTGATCTAACATGCGTTATAAGATATCCTTGCagag                                                                                         >  2:299272/1‑141 (MQ=255)

                                                      aCGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGGTATCCTTGCAGAggg                                                                                       >  2:601395/1‑141 (MQ=255)

                                                      aCGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAggg                                                                                       >  2:319679/1‑141 (MQ=255)

                                                       cgcgGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAAGGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGt                                                                                      <  1:160693/141‑1 (MQ=255)

                                                         cgGTGGCGACCAACTTGCAATTAACAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGa                                                                                    <  1:511808/141‑1 (MQ=255)

                                                          ggTGGCGGCCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGAc                                                                                   <  1:373824/141‑1 (MQ=255)

                                                            tGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaa                                                                                 <  1:924205/141‑1 (MQ=255)

                                                             ggCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaaa                                                                                <  1:350543/141‑1 (MQ=255)

                                                             ggCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACaaa                                                                                >  1:322839/1‑141 (MQ=255)

                                                              gCGACCAAGTTGCAATTAAAAGTATTCATGGTAATGGGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGGTCTCGCATGCGTTAAAAGATATCCTTGCAGAGGGGGGCAAAg                                                                               >  2:564490/1‑141 (MQ=255)

                                                               ggACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCGGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                                              <  2:566314/140‑1 (MQ=255)

                                                               cGACCAAGTTGCAGTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                                              <  2:860492/141‑1 (MQ=255)

                                                               cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACACAGt                                                                              >  1:998862/1‑141 (MQ=255)

                                                               cGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGt                                                                              >  1:476745/1‑141 (MQ=255)

                                                                  ccAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGCTCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCAt                                                                           <  2:150388/141‑1 (MQ=255)

                                                                          cAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGGGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATgg                                                                   <  1:761901/141‑1 (MQ=255)

                                                                            aTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGa                                                                 >  2:752478/1‑141 (MQ=255)

                                                                            aTTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGa                                                                 >  1:702097/1‑141 (MQ=255)

                                                                             ttAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGGTGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGGGACAAAGTCATTATCATGGGAc                                                                <  2:342674/141‑1 (MQ=255)

                                                                                 aaGTATTAATGGTAATGTGCGTTTCTATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATaa                                                            <  1:1184972/141‑1 (MQ=255)

                                                                                 aaGTATTAATGGTAATGGGCGTTTCTATGGCGGTAAGCCTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATaa                                                            <  2:930427/141‑1 (MQ=255)

                                                                                    tATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGCAACGTACGCTTGGAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACg                                                         >  1:1123648/1‑141 (MQ=255)

                                                                                     aTTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACGTGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGt                                                        >  2:789661/1‑141 (MQ=255)

                                                                                      ttAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTc                                                       >  1:802134/1‑141 (MQ=255)

                                                                                        aaTGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCt                                                     >  2:223344/1‑141 (MQ=255)

                                                                                                 gtgCGTTTCTATGGCGGTACGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGAt                                            >  1:634122/1‑141 (MQ=255)

                                                                                                 gtgCGTTTCTATGGAGGTAAGACTGACCCAATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGAt                                            >  1:638702/1‑141 (MQ=255)

                                                                                                  tgCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGGTCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATg                                           >  2:1028930/1‑141 (MQ=255)

                                                                                                  tgCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATg                                           <  1:1144246/141‑1 (MQ=255)

                                                                                                    cGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCa                                         >  1:740930/1‑141 (MQ=255)

                                                                                                    cGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCa                                         >  1:418399/1‑141 (MQ=255)

                                                                                                      tttCTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt                                       <  1:708009/141‑1 (MQ=255)

                                                                                                      tttCTATGACGGTAAGACTTACCCGATGGAGAAACCTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt                                       <  1:745372/141‑1 (MQ=255)

                                                                                                      tttCTATGACGGGAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAt                                       <  1:641111/141‑1 (MQ=255)

                                                                                                         cTATGGCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTgg                                    >  2:794616/1‑141 (MQ=255)

                                                                                                          tATGACGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGt                                   >  2:955082/1‑141 (MQ=255)

                                                                                                             ggCGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTgca                                >  1:308268/1‑141 (MQ=255)

                                                                                                               cGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTgcagc                              <  1:1100500/141‑1 (MQ=255)

                                                                                                               cGGTAAGACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTgcagc                              <  1:501618/141‑1 (MQ=255)

                                                                                                                     gACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCgg                        >  2:1161074/1‑141 (MQ=255)

                                                                                                                     gACTGACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCGGACTTAGATGCAATTGGTGCAGCAATCgg                        >  2:1072009/1‑141 (MQ=255)

                                                                                                                     gACTGACCCGATGGAGAAACGTACTCGTGTAAGAGTACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCGATTGGTGCAGCAATCgg                        <  2:152548/141‑1 (MQ=255)

                                                                                                                     gACTGACAGGATGGAGAAACGTATTCGTGTAGGAGCCCGTGTGATCTCCCATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATGATGGGACATAAACGTCCTGACTTAGTTGCAATTGGTGCAGAAATggg                        >  2:520263/1‑141 (MQ=255)

                                                                                                                         gcaccGATGTAGAAACGTACGGGTGTAAGAGCACGTGTGATCTCACATTCGTTAAAAGATATCCTTGCAGATGGTGACAAATTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGgtgtg                    <  1:707455/138‑1 (MQ=255)

                                                                                                                         gACCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGgtgtg                    <  1:782952/141‑1 (MQ=255)

                                                                                                                         gACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGgtgtg                    <  2:1196721/141‑1 (MQ=255)

                                                                                                                         gACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGgtgtg                    <  2:708139/141‑1 (MQ=255)

                                                                                                                         gACCCGATGCAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAATATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGgtgtg                    <  2:885260/141‑1 (MQ=255)

                                                                                                                          aCCCGATGGAGAAACGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGgtgtgt                   >  1:189460/1‑141 (MQ=255)

                                                                                                                             cGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTa                >  2:702828/1‑141 (MQ=255)

                                                                                                                               aTGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACAAGCGTTAAAAGATATCATTGCAGAGGGTGACAAAGTCATTAGCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGCGTGTCTAGa              >  1:1007820/1‑141 (MQ=255)

                                                                                                                                  gagaAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGGTATCCTTGCAGATGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAAATGGTGCAGAAATCGGTGTGTCTAGAttt           >  2:52404/1‑141 (MQ=255)

                                                                                                                                    gaAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGc         <  2:50141/141‑1 (MQ=255)

                                                                                                                                     aaaCGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGGGACACAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGATGAAGCAATTGGGGTGTCACGATTTGcc        >  2:390854/1‑140 (MQ=255)

                                                                                                                                       aCGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGCAat      >  1:449343/1‑141 (MQ=255)

                                                                                                                                        cGTATTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGCAatg     >  1:441935/1‑141 (MQ=255)

                                                                                                                                        cGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGCAatg     <  1:803004/141‑1 (MQ=255)

                                                                                                                                          tACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGCAatgat   >  2:570814/1‑141 (MQ=255)

                                                                                                                                           aCTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGGAGAGGGTGACAAAGTCATTATCATGGGACAGAAACGTCCTGACTTAGATGCAATTGGTTCGGCAAGCGGTGTGTCTAGATTTGCAatgatg  >  1:219168/1‑141 (MQ=255)

AAATTTAATCGACTTAGGTGAATTATCACAATCAGGCCTAGACTTAGCATTAGGACGCGGTGGCGACCAAGTTGCAATTAAAAGTATTAATGGTAATGTGCGTTTCTATGGCGGTAAGACTGACCCGATGGAGAAACGTACTCGTGTAAGAGCACGTGTGATCTCACATGCGTTAAAAGATATCCTTGCAGAGGGTGACAAAGTCATTATCATGGGACATAAACGTCCTGACTTAGATGCAATTGGTGCAGCAATCGGTGTGTCTAGATTTGCAATGATG  >  USA300TCH1516_ALE/19154‑19433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A