Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F109 I0 R1 76 110.5 2404748 96.5% 2320581 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 478,502 C→A 71.0% D342E (GAC→GAA)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE478,5020CA71.0% 173.5 / 107.6 141D342E (GAC→GAA) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base C (17/24);  new base A (45/54);  total (63/78)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 142 total aligned reads displayed.

AGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAA  >  USA300TCH1516_ALE/478364‑478641
                                                                                                                                           |                                                                                                                                           
aGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGAATCTGCT‑AAAGACGc                                                                                                                                           >  2:336693/1‑141 (MQ=255)
  gtCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCtt                                                                                                                                         <  1:503292/140‑1 (MQ=255)
   tCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTa                                                                                                                                        <  2:321744/141‑1 (MQ=255)
   tCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTa                                                                                                                                        <  1:49610/141‑1 (MQ=255)
     aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATg                                                                                                                                      >  2:58405/1‑141 (MQ=255)
       tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGc                                                                                                                                    >  2:622771/1‑141 (MQ=255)
       tgtgCATTGGGGGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTTAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGc                                                                                                                                    <  1:917265/141‑1 (MQ=255)
        ttgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCt                                                                                                                                   <  2:765889/140‑1 (MQ=255)
        ttgCATTGGGTGTATATTCAGCAGAGAATATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCt                                                                                                                                   <  2:263891/140‑1 (MQ=255)
        gtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCt                                                                                                                                   <  1:933558/141‑1 (MQ=255)
          gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTgg                                                                                                                                 <  1:354579/141‑1 (MQ=255)
           cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGCTTCAATATTCCAAAACAAGCATCTGCG‑AAAGAAGCTTATGGCTGGa                                                                                                                                >  1:103053/1‑141 (MQ=255)
           cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGa                                                                                                                                <  1:451683/141‑1 (MQ=255)
           cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGAAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGa                                                                                                                                >  1:8067/1‑141 (MQ=255)
             ttGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGAtt                                                                                                                              <  2:152072/141‑1 (MQ=255)
              tGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCCAAACAAGCATCTGTT‑AAAGAGGCTTATGGCTGGATTg                                                                                                                             >  1:157230/1‑141 (MQ=255)
              tGGGTGTATATTCAGCAGCGACGGCACATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTg                                                                                                                             >  2:850409/1‑141 (MQ=255)
               gggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGt                                                                                                                            >  2:1004553/1‑141 (MQ=255)
               gggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGt                                                                                                                            >  1:253262/1‑141 (MQ=255)
                  gttataTTCGGCGGCGATTACTCATTTAATATTGCACGTTATTTTTAAAGCAACATTATTTTTACAATCAGTTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCAt                                                                                                                         <  2:106138/139‑1 (MQ=255)
                     atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATggg                                                                                                                      <  2:46701/141‑1 (MQ=255)
                      tatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGa                                                                                                                     <  2:473682/141‑1 (MQ=255)
                        ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                   <  1:973769/141‑1 (MQ=255)
                           agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACgtgt                                                                                                                >  2:797126/1‑141 (MQ=255)
                           agcagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACgtgt                                                                                                                >  2:796675/1‑141 (MQ=255)
                           agcagcGATGATTCAGTTTATATTGCACGGTATTTTTAAAGCAACATTATTTCTAAAGACATTTACGATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTCATGGCTGGCTTGTCATGGGACgtgt                                                                                                                <  1:274883/141‑1 (MQ=255)
                             cagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAt                                                                                                              >  1:636983/1‑141 (MQ=255)
                                 gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCG‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                          >  1:41400/1‑141 (MQ=255)
                                   ttattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                        <  1:356290/141‑1 (MQ=255)
                                   ttattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                        <  1:959736/141‑1 (MQ=255)
                                    tattGATTTAATATTGGCTGGTATTTTTAAAGCAACATTGTGTTTACACTCAGGTTCTATCGTTAAGCGATTCAATATTCCAAAACAAGCCTCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                       <  2:237510/141‑1 (MQ=255)
                                    tattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                       <  2:237217/141‑1 (MQ=255)
                                    tattCATTTAAGATTGCACGGTAGTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                       <  2:950535/141‑1 (MQ=255)
                                      ttCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtattt                                                                                                     >  1:966834/1‑140 (MQ=255)
                                       tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCCT‑TACTGAAAGAAGCTTCTGGCTGGATTGTCATGGGACGTGTATTAGCtattat                                                                                                    >  1:865498/1‑141 (MQ=255)
                                       tCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATAAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtattat                                                                                                    >  1:230071/1‑141 (MQ=255)
                                         aTTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCg                                                                                                  >  1:1130636/1‑141 (MQ=255)
                                         aTTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATTATCg                                                                                                  >  1:177093/1‑141 (MQ=255)
                                          tttAATATTGCACGCTATTCTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATGATCGt                                                                                                 >  1:657479/1‑141 (MQ=255)
                                           ttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTg                                                                                                <  2:264516/141‑1 (MQ=255)
                                            tAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATTGGACGTGTATTAGCTATTATCGtcg                                                                                               >  2:119745/1‑139 (MQ=255)
                                              atatTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCa                                                                                             <  1:1156655/141‑1 (MQ=255)
                                               tatTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAt                                                                                            <  1:1090105/141‑1 (MQ=255)
                                                atTGCGCGGTTTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                           <  2:596695/141‑1 (MQ=255)
                                                atTGCACGGTATTTTTAAAGAAACATTATTTTTCCATTAACGTTTTTTCGTGAAGCGATTCAATATTCAAAAACAAGCATCTGCT‑AAAGAAGCTTATGTCGGGATTGTCATGGGGCGTGTATTAGCTATTATCGTGGCAtt                                                                                           >  2:939739/1‑141 (MQ=255)
                                                  ttccccgTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                         <  2:82706/135‑1 (MQ=255)
                                                  tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                         <  1:22938/141‑1 (MQ=255)
                                                      cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATGATCGTGGCATTTCTAtt                                                                                     >  2:113538/1‑141 (MQ=255)
                                                         tATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTGATGGGACGTGTATTAGCTATTATGGTGGCATTTTTATTTTg                                                                                  >  2:684843/1‑141 (MQ=255)
                                                          aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                                 <  1:272093/141‑1 (MQ=255)
                                                            ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                               <  2:1033660/141‑1 (MQ=255)
                                                            ttttAAAGCAACATTATTTATACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                               >  1:894251/1‑141 (MQ=255)
                                                            ttttAAAGCAACAGTATTTTTACAATCAGCTTTTATGTTGAAGCGATAAGAAATTTATAATGAATCTGCGTCA‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCAATTATCGTGGGCTTTCTATTTTGGAt                                                                               >  2:1151195/1‑141 (MQ=255)
                                                             tttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                              >  2:522611/1‑141 (MQ=255)
                                                             tttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGAATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAGTAGCTATTATCGTGGCATTTCTGTTTTGGATg                                                                              >  2:400148/1‑141 (MQ=255)
                                                                   gCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagtagt                                                                        <  1:1158616/141‑1 (MQ=255)
                                                                     acctttattTCTACAATCAGGTTCTAGCGTGAAGTGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                      <  2:821524/137‑1 (MQ=255)
                                                                     aaCATTATTTTTACACTCAGGTTCTATCGGGAAGCGATTCAATATTCCAAAACCAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                      <  1:431936/141‑1 (MQ=255)
                                                                      aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCCTTTCTATTTTGGATGAGTAGGGAc                                                                     >  1:189742/1‑141 (MQ=255)
                                                                      aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGACTAGGGAc                                                                     >  2:23320/1‑141 (MQ=255)
                                                                       cATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACa                                                                    >  1:300890/1‑141 (MQ=255)
                                                                         ttattTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTACTTTGGATGAGTAGTGACAGa                                                                  <  1:723846/141‑1 (MQ=255)
                                                                          tattTTTACAATCAGTTTCTATCGTGAAGCGATTCAATATTCCAAATCAAGCATCTGCT‑AAAGAAGTTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGaa                                                                 <  2:832635/141‑1 (MQ=255)
                                                                           attTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAg                                                                >  1:542778/1‑141 (MQ=255)
                                                                             ttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                              >  1:942336/1‑141 (MQ=255)
                                                                               ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCa                                                            <  1:850409/141‑1 (MQ=255)
                                                                                 aCAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCC‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                          <  1:990507/141‑1 (MQ=255)
                                                                                   attCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATg                                                        <  1:1093827/139‑1 (MQ=255)
                                                                                    aTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGa                                                       >  2:1074421/1‑141 (MQ=255)
                                                                                     tCAGGTTCTATCGTGAAGCGATTCGATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTAGGGGTGGATTGTCATGGGACGTGTATTAGCGATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                      <  2:156916/141‑1 (MQ=255)
                                                                                      aagtttCTATCGTAAAGCGATTAAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                     <  2:157230/137‑1 (MQ=255)
                                                                                            cTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAATGCATATGAAGTGTTac                                               >  1:485309/1‑140 (MQ=255)
                                                                                             tATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAg                                              <  2:223560/141‑1 (MQ=255)
                                                                                             tATCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAg                                              <  2:751672/141‑1 (MQ=255)
                                                                                              aTCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                             <  2:1031801/141‑1 (MQ=255)
                                                                                               tCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGCGACAGAACTGCATATGAAGTGTTAAGTg                                            <  1:774026/141‑1 (MQ=255)
                                                                                                  tGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAc                                         >  1:757548/1‑141 (MQ=255)
                                                                                                    aaGTGATTTGATATTGCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGGGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTc                                       <  1:433499/141‑1 (MQ=255)
                                                                                                      gCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGCAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATAATGGCATGGCTAGTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                     <  1:571716/141‑1 (MQ=255)
                                                                                                       cGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGGACTCAtt                                    >  2:184156/1‑141 (MQ=255)
                                                                                                           tCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAg                                <  2:608607/141‑1 (MQ=255)
                                                                                                             aaTATTCCAAAACAAGAATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGa                              >  1:591980/1‑141 (MQ=255)
                                                                                                              atatTCCAAAACCAGCATCGGCT‑AAAGAAGCTTATGGCTGGATTGGCATGGGACGTGTATCCGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTCGGAt                             <  2:895872/141‑1 (MQ=255)
                                                                                                              atatTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGAt                             <  2:726089/141‑1 (MQ=255)
                                                                                                               tatTCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATg                            >  2:1078234/1‑141 (MQ=255)
                                                                                                                atTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                           <  1:336432/141‑1 (MQ=255)
                                                                                                                 ttCCAAAACAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGt                          >  2:762443/1‑141 (MQ=255)
                                                                                                                       aaCAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTAc                    <  1:384474/141‑1 (MQ=255)
                                                                                                                        aCAAGCATTCGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGAGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACt                   <  1:803908/141‑1 (MQ=255)
                                                                                                                        aCAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTGGGATAAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACt                   >  1:1177473/1‑141 (MQ=255)
                                                                                                                         tctgtaTCTGAT‑ACAGAAGCTTACGGCTTGCTTTCCTTGGGACGAGCATTGGCTATTAGCGTGGTATTTCTATTTTGGATGAGTAGTGCCACAAGTGCATATGACGCGTTAAGTGCACTCATTCTAGGCTGGTCATTACtt                  <  2:931206/136‑1 (MQ=255)
                                                                                                                         cAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACtt                  <  1:257641/141‑1 (MQ=255)
                                                                                                                         cAAGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACtt                  >  2:940623/1‑141 (MQ=255)
                                                                                                                          aaGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg                 <  2:387792/141‑1 (MQ=255)
                                                                                                                          aaGCATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg                 >  2:1175780/1‑141 (MQ=255)
                                                                                                                            gCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCTTTACTTGTa               >  1:1033604/1‑141 (MQ=255)
                                                                                                                            gCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa               >  1:1033170/1‑141 (MQ=255)
                                                                                                                             cATCTGCT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTAt              >  2:699091/1‑141 (MQ=255)
                                                                                                                                   cT‑AAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGCTGGTCATTACTTGTATCTTGGa        >  2:1017724/1‑141 (MQ=255)
                                                                                                                                          gcGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCaaa  <  1:431626/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT‑AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCAAA  >  USA300TCH1516_ALE/478364‑478641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A