Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F52 I0 R1	39	94.7	2129336	96.2%	2048421	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	665,392	T→G	84.0%	L79* (TTA→TGA)	paiA_1 →	Spermidine/spermine N(1)‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	665,392	0	T	G	84.0%	209.9 / 24.8	84	L79* (TTA→TGA)	paiA_1	Spermidine/spermine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (8/6); new base G (38/32); total (46/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

CGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAA  >  USA300TCH1516_ALE/665254‑665528

cGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgat                                                                                                                                        >  1:902076/1‑141 (MQ=255)

cGACAACAGTTAATCACTTGGTAGAAGCTGCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgat                                                                                                                                        >  1:213447/1‑141 (MQ=255)

 gACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTGGCTAACTTTATTTACGGTGAAGAATgatt                                                                                                                                       >  1:10469/1‑140 (MQ=255)

 cacaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgata                                                                                                                                       <  1:375940/140‑1 (MQ=255)

  acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                      >  2:64234/1‑141 (MQ=255)

   caacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                     <  1:198933/141‑1 (MQ=255)

    aacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAGGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATGttt                                                                                                                                    >  1:901542/1‑141 (MQ=255)

     acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTa                                                                                                                                   >  1:936225/1‑141 (MQ=255)

     acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTa                                                                                                                                   >  1:963533/1‑141 (MQ=255)

     acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTa                                                                                                                                   >  2:257759/1‑141 (MQ=255)

        gTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCa                                                                                                                                <  2:103084/141‑1 (MQ=255)

        gTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCa                                                                                                                                <  2:103117/141‑1 (MQ=255)

          tAATCACTTGTTAGAATCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGc                                                                                                                              <  1:577685/141‑1 (MQ=255)

          tAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGc                                                                                                                              <  1:764268/141‑1 (MQ=255)

          tAATCACTTGTTAGAAGCTTCATATAATGAACAGCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGc                                                                                                                              <  2:295749/141‑1 (MQ=255)

               aCTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCAtt                                                                                                                         <  1:104492/141‑1 (MQ=255)

                cctataaGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCACATTa                                                                                                                        <  2:236787/135‑1 (MQ=255)

                cTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTa                                                                                                                        >  2:550731/1‑141 (MQ=255)

                    ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGtt                                                                                                                    <  1:816174/141‑1 (MQ=255)

                    ttAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGtt                                                                                                                    <  2:717389/141‑1 (MQ=255)

                     tAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTa                                                                                                                   <  1:206961/141‑1 (MQ=255)

                     tAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGCTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTa                                                                                                                   <  1:715549/141‑1 (MQ=255)

                          gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCa                                                                                                              >  1:160968/1‑141 (MQ=255)

                          aattaatatAACGCACATCATTTAAAGAAAAGACTTCCAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCa                                                                                                              <  1:220420/136‑1 (MQ=255)

                                          atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGCATCGCAACATACAg                                                                                              >  1:929295/1‑141 (MQ=255)

                                          atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                              >  1:319286/1‑141 (MQ=255)

                                            catTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGGTATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGt                                                                                            >  1:457082/1‑141 (MQ=255)

                                            catTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGt                                                                                            >  1:294071/1‑141 (MQ=255)

                                              tttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTa                                                                                          >  1:778032/1‑141 (MQ=255)

                                              tttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTa                                                                                          >  2:604733/1‑141 (MQ=255)

                                              tttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATAGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTa                                                                                          >  2:342877/1‑141 (MQ=255)

                                               ttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                         >  1:1034798/1‑141 (MQ=255)

                                                tAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATg                                                                                        >  1:102186/1‑141 (MQ=255)

                                                tAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATg                                                                                        >  1:416619/1‑141 (MQ=255)

                                                tAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTAAGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGGATCGAAACAAACAGGTTATg                                                                                        >  2:445818/1‑141 (MQ=255)

                                                 aaagaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATgg                                                                                       >  1:456913/1‑141 (MQ=255)

                                                  aagaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGt                                                                                      >  1:365648/1‑141 (MQ=255)

                                                   agaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTa                                                                                     <  1:974463/141‑1 (MQ=255)

                                                    gaaaagtCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGCGAATGCCATCGTTGGATTTGCTAACTTTTTTTACGGTTAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACATGTTATGGTAc                                                                                    <  2:425178/141‑1 (MQ=255)

                                                      aaataCTTCAAGAACATTTATTCTTAGTCGTTTAAGAAAGTAATGACATCGTTGTCTTTGCTAATTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGTAACATACAGGTTATGGTACAg                                                                                  <  2:474338/141‑1 (MQ=255)

                                                       aagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGc                                                                                 >  1:881244/1‑141 (MQ=255)

                                                       aagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGc                                                                                 >  1:899478/1‑141 (MQ=255)

                                                        agaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCa                                                                                <  2:683005/141‑1 (MQ=255)

                                                         gaCTTCAAGAACAATTATTCTTAGTCGTTGTAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCAt                                                                               <  2:672758/141‑1 (MQ=255)

                                                           cTTCAAGAACATGTTTGCTTAGTCGGTGAAGCAGGTAATGACAGCGTTGGCTTTGCTAACTTTATGTACGGTGAAGACTGATATTTATCAGCTCAGTATGTTAAATCAGAATGGTAACATACAGGTTATGGTACAGCATTg                                                                             <  2:317685/141‑1 (MQ=255)

                                                           cTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTg                                                                             <  2:800785/141‑1 (MQ=255)

                                                           cTTCAAGAACAATTATGCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATGATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTg                                                                             <  2:1030877/141‑1 (MQ=255)

                                                                aGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaaa                                                                        <  1:875793/141‑1 (MQ=255)

                                                                 gAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAAt                                                                       >  2:871092/1‑141 (MQ=255)

                                                                       ttattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCCACTCATTGTGTTAAACCAGATTCGCAGCATACAGGTTATGGTACAGCTTTATTAAATGAAGGa                                                                 >  1:772111/1‑141 (MQ=255)

                                                                       ttattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGa                                                                 >  1:847149/1‑141 (MQ=255)

                                                                         attCTTAGTGGTTGAGGAAGGTAATGACATCATTGGCTTTGCTAACTTTATTTCCGTTGAAGACTTATATTTATCAGATCATTATGTTAAACAAGAATCGCACAATAGATGTGAATGTGCAGACTTTTTAAATGAAGGagt                                                               >  2:318398/1‑139 (MQ=255)

                                                                         attCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCAGTGTTAAATGAAGGAtt                                                               >  2:644224/1‑141 (MQ=255)

                                                                         attCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGGTATGGTACAGCATTGTTAAATGAAGGagt                                                               >  1:323633/1‑139 (MQ=255)

                                                                          ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTa                                                              >  2:1046986/1‑141 (MQ=255)

                                                                           tCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACGTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTAt                                                             <  2:134532/141‑1 (MQ=255)

                                                                            cTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATc                                                            <  1:523454/141‑1 (MQ=255)

                                                                            cTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGTGACAGCATTGTTAAATGACGGATTATc                                                            >  2:909418/1‑141 (MQ=255)

                                                                             ttAGTCGTTGTAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCCCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCa                                                           <  1:7214/141‑1 (MQ=255)

                                                                             ttAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCa                                                           <  2:546565/141‑1 (MQ=255)

                                                                                gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTAtaccgt                                                        >  1:62331/1‑136 (MQ=255)

                                                                                gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGt                                                        >  1:870908/1‑141 (MQ=255)

                                                                                gTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGt                                                        <  1:566250/141‑1 (MQ=255)

                                                                                       aagaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGATGAATTATATTTATCAGCTCATTATGTTAAATCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAg                                                 <  2:257165/141‑1 (MQ=255)

                                                                                       aagaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAg                                                 <  1:820927/141‑1 (MQ=255)

                                                                                        agaagGTAATGACATCATTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATGGTTAAATGAAGGATTATCACGTTTTGAAGa                                                >  1:632186/1‑141 (MQ=255)

                                                                                         gaagGTAATGACATCGTTGGCTTTGCTAACTTTGTTTACGGTGAAGAATGATGTTTATCAGCTCGTTATGTTAAACCAGAATCGCAACATACAGGTTATGGTTCAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                               >  1:1015690/1‑141 (MQ=255)

                                                                                         gaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTGTGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                               >  1:603968/1‑141 (MQ=255)

                                                                                         gaagGTAATGACATCGTTGGCTGTGCTCACTTTATTTACGGTGAAGAATGATATTTATAAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGGATTGTTAAATGAAGGATTATCACGTTTTGAAGAt                                               >  2:440031/1‑141 (MQ=255)

                                                                                             gTAATGTCATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAAAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAt                                           <  2:254082/141‑1 (MQ=255)

                                                                                                 tGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGa                                       <  2:858891/141‑1 (MQ=255)

                                                                                                  gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                      >  2:106429/1‑141 (MQ=255)

                                                                                                     aTCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGgt                                   >  2:316939/1‑141 (MQ=255)

                                                                                                      tCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGgtg                                  <  1:891774/141‑1 (MQ=255)

                                                                                                       cGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTTCAGCTGTTGGAAATGAAGGTTTATCACGTTTTGAAGATAAATTTGAAGgtgt                                 >  1:241336/1‑141 (MQ=255)

                                                                                                       cGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGgtgt                                 <  1:257759/141‑1 (MQ=255)

                                                                                                         ttGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTGTTGAAGATAAATTTGAAGGTGttt                               >  1:94891/1‑141 (MQ=255)

                                                                                                           ggCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTTAAGGTGTTTaa                             >  2:787983/1‑140 (MQ=255)

                                                                                                           ggCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTAc                             >  2:916294/1‑141 (MQ=255)

                                                                                                                  cTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGGAGATAAATTTGAAGGTGTTTACTTAGAAg                      >  1:937065/1‑141 (MQ=255)

                                                                                                                     aCTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAg                   <  1:297156/141‑1 (MQ=255)

                                                                                                                     aCTTTATATACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAGATGATGGATGAGCTCGTTTTGAAGATAAATATGAAGGGGTTTACTTAGAAATAg                   >  2:746785/1‑141 (MQ=255)

                                                                                                                             taCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaa           <  1:759896/141‑1 (MQ=255)

                                                                                                                               cGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaaaa         >  1:904802/1‑141 (MQ=255)

                                                                                                                                ggTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAAt        <  1:300898/141‑1 (MQ=255)

                                                                                                                                 gTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATg       <  1:492479/141‑1 (MQ=255)

                                                                                                                                     agaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaaga   >  1:609495/1‑141 (MQ=255)

                                                                                                                                      gaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaagaa  <  2:319335/141‑1 (MQ=255)

CGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGAA  >  USA300TCH1516_ALE/665254‑665528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A