Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F104 I0 R1	54	57.6	1336546	96.4%	1288430	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	665,392	T→G	100%	L79* (TTA→TGA)	paiA_1 →	Spermidine/spermine N(1)‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	665,392	0	T	G	100.0%	293.5 / ‑6.5	84	L79* (TTA→TGA)	paiA_1	Spermidine/spermine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (42/41); total (42/42)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGA  >  USA300TCH1516_ALE/665254‑665527

cGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgat                                                                                                                                       >  1:107256/1‑141 (MQ=255)

cGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgat                                                                                                                                       >  2:444746/1‑141 (MQ=255)

  acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                     <  1:45716/141‑1 (MQ=255)

  acaacaGTTAAGCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTACTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                     <  1:647771/141‑1 (MQ=255)

   caacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                    <  1:600415/141‑1 (MQ=255)

    aacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAttt                                                                                                                                   >  2:531027/1‑141 (MQ=255)

     acaGGTAATCACGTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAAAATGTTATGCCTAAGTTACCACTGCTAACTTTATTTACGGTGAAGAATGATATTTa                                                                                                                                  <  1:319486/141‑1 (MQ=255)

        attAACCACTTGTTAGAAGCCTCATACAATGAACATCAATTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCCTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGACATTTATCa                                                                                                                               <  2:85560/140‑1 (MQ=255)

          tAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTAATTCAGGGGGAAGAATGATATTTATCAGc                                                                                                                             >  2:329014/1‑141 (MQ=255)

            aTCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTc                                                                                                                           >  1:408993/1‑141 (MQ=255)

            aTCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTc                                                                                                                           >  2:107866/1‑141 (MQ=255)

             tCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCa                                                                                                                          >  2:446574/1‑141 (MQ=255)

             tCACTTGCTAGACGCTGCATATAATGAACATCATTTAAAGAAAAGACTTAAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTAGCTTTGCTAACTTTATTTACGGTGAAGAATGATCGTTATCAGCTCa                                                                                                                          >  2:44812/1‑141 (MQ=255)

               aCTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCAtt                                                                                                                        >  2:366951/1‑141 (MQ=255)

                     tAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTa                                                                                                                  >  2:111064/1‑141 (MQ=255)

                      aGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTaa                                                                                                                 <  2:649347/141‑1 (MQ=255)

                      aGAAGCGTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTaa                                                                                                                 <  2:311436/141‑1 (MQ=255)

                         aGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAcc                                                                                                              <  1:522944/141‑1 (MQ=255)

                         aGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAcc                                                                                                              <  1:366951/141‑1 (MQ=255)

                             tCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGaa                                                                                                          >  1:659102/1‑141 (MQ=255)

                               atatAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTCGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATGTACGGTGAAGGATGATATTTATCACCTCATTATGTTAAACCAGAATc                                                                                                        >  1:386020/1‑141 (MQ=255)

                                tataATGAACATCATTTAAAGACAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCg                                                                                                       <  1:129157/141‑1 (MQ=255)

                                tataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCg                                                                                                       >  2:111873/1‑141 (MQ=255)

                                tataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACAGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCg                                                                                                       >  2:341231/1‑141 (MQ=255)

                                aataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCg                                                                                                       <  2:384355/140‑1 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTGGTCGTTGAAGAAGGTCATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTAAGTTAACCCAGAATCGc                                                                                                      >  1:244084/1‑141 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGCAGGTAATGACATCGTTGGCTTTGCTCCCTTTATTTACGGTGAAGAATGATATTTATCACCTGATTATGTTAAACAAAAATCGc                                                                                                      >  1:245669/1‑141 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCgg                                                                                                      >  2:634306/1‑140 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCga                                                                                                      >  2:518737/1‑140 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                      >  2:360417/1‑141 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                      >  1:221167/1‑141 (MQ=255)

                                 ataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                      <  1:280855/141‑1 (MQ=255)

                                      gAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACAt                                                                                                 <  2:255344/141‑1 (MQ=255)

                                       aaCATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATa                                                                                                <  1:107866/141‑1 (MQ=255)

                                       aaCATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATa                                                                                                <  2:408993/141‑1 (MQ=255)

                                          atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                             >  2:333951/1‑141 (MQ=255)

                                          atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGATAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGGATCCCAAAATACAg                                                                                             >  2:96942/1‑141 (MQ=255)

                                           tcatTTAAAGAGAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                            <  1:104176/141‑1 (MQ=255)

                                               ttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTAt                                                                                        >  1:350718/1‑141 (MQ=255)

                                                tAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATAAAGGTTATg                                                                                       >  2:43749/1‑141 (MQ=255)

                                                       aagaCTTCAAGAATAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCTTTATCTTGTCTAACTTTACTTACGGTGAAGAATTATATTTATTAGTTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGc                                                                                <  2:1645/141‑1 (MQ=255)

                                                            ttCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGt                                                                           <  2:403041/141‑1 (MQ=255)

                                                            ttCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGt                                                                           <  2:403044/141‑1 (MQ=255)

                                                              cAAGAACAATTATTCTTAGTCGTTGACGAAGGTAATGACATCGTTGGCTTTGCTAACTTTAGTTAAGGTGAAGAATGATATGTATCAGCTCATTCTGTTAAACCAGACTGTCAACATTGAGGTTATGGTACAGCCTTGTTa                                                                         >  2:184547/1‑141 (MQ=255)

                                                                aGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaaa                                                                       <  1:444746/141‑1 (MQ=255)

                                                                aGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaaa                                                                       >  1:435733/1‑141 (MQ=255)

                                                                     aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTCACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                  <  1:126122/141‑1 (MQ=255)

                                                                     aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                  <  2:107256/141‑1 (MQ=255)

                                                                     aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                                  >  1:158633/1‑141 (MQ=255)

                                                                       ttattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGa                                                                <  1:329014/141‑1 (MQ=255)

                                                                         attCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGTTCATTATGTTAAACCAGAATCGCAACTTACAGGTTATGGTAAAGCATTGTTAAATGAAGGAtt                                                              <  1:111873/141‑1 (MQ=255)

                                                                         attCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTATAGCATTGTTAAATGAAGGAtt                                                              <  1:341231/141‑1 (MQ=255)

                                                                          ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTa                                                             >  2:553908/1‑141 (MQ=255)

                                                                          ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTa                                                             >  2:270030/1‑141 (MQ=255)

                                                                          ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTa                                                             >  2:310937/1‑141 (MQ=255)

                                                                          ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTa                                                             >  2:311031/1‑141 (MQ=255)

                                                                          ttCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGAATTGTTAAATGAAGGAttt                                                             >  2:297776/1‑140 (MQ=255)

                                                                           tCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTCTTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTaa                                                            >  2:427075/1‑140 (MQ=255)

                                                                           tCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACACGGTACGGTACAGCATTGTTAAATGAAGGATTAt                                                            >  2:578458/1‑141 (MQ=255)

                                                                           tCTTAGTCGTTGAAGAAGGGAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTAt                                                            >  2:5966/1‑141 (MQ=255)

                                                                            cTTAGTCTTTGAAAAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATCGTACAGCATTGTTAAATGAAGGATTATc                                                           <  2:201248/141‑1 (MQ=255)

                                                                            cTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATc                                                           <  1:65948/141‑1 (MQ=255)

                                                                            cTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCCCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATc                                                           <  2:355725/141‑1 (MQ=255)

                                                                              tAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTAGCTAACTTTATTTACGGGGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGAAACATACAGGTTATCGTACAGCATTGTGAAAGGAAGGATTATccc                                                         >  2:346545/1‑139 (MQ=255)

                                                                                    ttGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGGTAATAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTg                                                   <  2:106753/141‑1 (MQ=255)

                                                                                     tGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGa                                                  <  2:212131/141‑1 (MQ=255)

                                                                                      gaagaaGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTACACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGaa                                                 <  2:16282/141‑1 (MQ=255)

                                                                                      gaagaaGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGaa                                                 >  1:86188/1‑141 (MQ=255)

                                                                                               aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                        >  1:649908/1‑141 (MQ=255)

                                                                                               aaTGACATCGTTGGCTTTGCTAACGTTAGTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                        >  1:423102/1‑141 (MQ=255)

                                                                                                    aaTCGTGTGCTTTTCTAACTTTATTTACAGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGAAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAgg                                   <  2:31974/140‑1 (MQ=255)

                                                                                                        gTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGtt                               <  2:647192/141‑1 (MQ=255)

                                                                                                        gTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGtt                               <  1:578722/141‑1 (MQ=255)

                                                                                                               ttGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGTATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAg                        <  2:520762/141‑1 (MQ=255)

                                                                                                                  cTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGagg                     >  1:233261/1‑139 (MQ=255)

                                                                                                                       tttatttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                <  1:407143/141‑1 (MQ=255)

                                                                                                                       tttatttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                <  2:659102/141‑1 (MQ=255)

                                                                                                                       tttatttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                <  1:416450/141‑1 (MQ=255)

                                                                                                                       gttatttaCGGTGTAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATTGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGat                <  1:166948/140‑1 (MQ=255)

                                                                                                                           tttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCATCATACTGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGACAAATTTGAAGGTGTTTACGTAGAAGTAGataata            >  1:404197/1‑141 (MQ=255)

                                                                                                                           tttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGATGGTGTTTACTTAGAAGTAGataata            <  1:333951/141‑1 (MQ=255)

                                                                                                                               cGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATAGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaaaa        >  2:570604/1‑141 (MQ=255)

                                                                                                                                ggTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAAt       <  1:111064/141‑1 (MQ=255)

                                                                                                                                   gaagaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaa    >  1:209998/1‑141 (MQ=255)

                                                                                                                                     agaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaaga  >  2:482171/1‑141 (MQ=255)

                                                                                                                                     agaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaaga  <  1:563590/141‑1 (MQ=255)

CGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGA  >  USA300TCH1516_ALE/665254‑665527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A