Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F9 I0 R1
|
58 |
62.8 |
1514203 |
84.7% |
1282529 |
220.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,909,229 |
A→C |
32.3% |
G146G (GGT→GGG) |
pyrG ← |
CTP synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,909,229 | 0 | A | C | 32.3%
| 28.7
/ 2.9
| 29 | G146G (GGT→GGG) | pyrG | CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base C (3/7); total (13/16) |
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGG > NC_000913/2909002‑2909457
|
gCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCa > 1:548225/1‑228 (MQ=255)
caggtgcgttcgCCGGAACAGCGGGATGTGAACGAAAAAGCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGGGTGGTGAGTCGGTTTGGTTTTGACTGACCCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCAcc < 2:273681/223‑1 (MQ=255)
gAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGATTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAACCAGAGTGGGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCCCTTCGAGGCAAGGCAAGGATTCGAAATCCCCTACTGTActcgcatt > 2:249159/1‑222 (MQ=255)
accAGCGCGATCTGAACGACAAACCAAGATGT‑CAGGCTTGATCACTATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTGAACTTCCCCAGCCGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGGGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGAAAGGATTCGATATCCCCTACTGTACCGCCGAtt < 1:683323/227‑1 (MQ=255)
tgctgtatGAACGATAAATCGGGATGT‑GAGGCTGGAGACCGATGGAGACCAGCTCTTTTACAGAGTGCTGAGTCGGTGTGGTTTTGACCTCCCCAGACGCTGCCATGGACGGCCCTCGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTCGCAAATCTCTTCAAGGAACGGCAAGGATTCTATATCCCCTACTGTACCGCCGATTTCTAc < 2:250850/222‑1 (MQ=255)
gtggatcgaaaCGACAAAGCAGGGTGT‑CAGGCTGGAACCCGATGGAGGGCAGCCTTTTTACAGAGTTCTGAGTGGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGTGGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTCCTGTACCGCCGATTTCTAc < 1:308604/220‑1 (MQ=255)
ggttgtCTTAACGACAAATCA‑GAGGTACGGGTTGGATACCGTTGGGGGGCAGCCTTTTTACAGAGTGCTGAGGCGGTTTGGTTTTGCTTACACCAGCCGTGGCCATTGACGGCACCAGCGTCAGGTGCTAAAACAGGGTGTGCTCACGGCCAATTTCAACAGCCATCGGGCGAATCGCTTCGAGGAACGGCAAGAATTCGATATCACCTACTGTACCGCCGATTTCTAc < 2:638557/224‑1 (MQ=255)
ggcgATCTGAACGACAAATCCGGATGG‑CAGGCTGGATTCCGATGGAGAGCAGCTCTTTTACAGAGTGCTGGGGCGGTTTGGTTTGGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTAc < 1:733796/228‑1 (MQ=255)
cgcgATCGGAACGAAAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTAc < 1:564212/229‑1 (MQ=255)
gAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCCGGTGCATAAACAGAGTGGGCTCACGGCCAATTTCAAAAGCCATCTGGCGAATCGCCTCGGGGAACGGCCAGGATTCGAATTACCCCACTTTACCGCCGCtttttccccatac > 2:184990/1‑217 (MQ=255)
cAAATCAGGATTG‑CAGGCTGGTACCCGATGGAGAGCCGCTTTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCCCCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCa < 1:229881/228‑1 (MQ=255)
aCCGATGGAGAGCAGTCTTTTTCCAGATTGCTTAGTCGGTTTGGTTTTGCCTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACGGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCTCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg < 1:2303/229‑1 (MQ=255)
agcagcTCTTTTACAGAGTTCTGGTTCGGTTTGTTTTGGACTTCACCAGACGCTGCCATTTACGCAACCAGCGTCAGTTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAAGCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGa < 1:581675/229‑1 (MQ=255)
agTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGGGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCACCACGCGCTCTTTGATTGCGCTAGTGCtgt > 1:640213/1‑228 (MQ=255)
tGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTCAGTCATGACCTTCCCCCCCTTCCAGCCCCGCCTCTTTCATTGCGTTAGGAAAGTGCg > 2:425672/1‑229 (MQ=255)
gtttggtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg < 1:364850/191‑1 (MQ=255)
gtttggtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg > 2:364850/1‑191 (MQ=255)
gtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCAATCTGGCGAATCGCTTCGAGGAACGGCAAGGCTTCGATATCACCTACTGTACCGCCGATTTCTACCAGTCCTACGTCATGAACTTCCCCCCCTTCCCGCACGCGCTCTTTTATTTCGTTTGTCATGTTCGGAAACAACTGCaacg > 2:487911/1‑226 (MQ=255)
cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTCCTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTCCGTTATTGATGTGCGGAATAACCTGCACGGTTCGCCCCAGGCAGTCACCGCGGCGTTCttt > 2:231018/1‑228 (MQ=255)
cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTCGGGATGTGCGGAATAACCTGCACGGTTGCGCCGTGGTAGTCACCGCGGCGGTCgtt > 1:588823/1‑228 (MQ=255)
cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCttt > 1:161828/1‑228 (MQ=255)
cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACAACGTCATGACCTTCGCCACCTTCCACCCCGCGCTCTTTGATTCCGTTAGGGACTGGCGGACTACCCTCCACGCTGCCGCCCTGGTAGTTCACGCGGCGGTAttt > 1:354108/1‑228 (MQ=255)
gcgcCAGGGCCATAAACAGAGTGGGCTCCCGGCCAATTTCAACAGCCATCGGGCGAATCGCTTCGAGAAACGGCAAGGATTCGATATCACCTACTGTCCCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAg < 1:700517/229‑1 (MQ=255)
gCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGGGGCGTTCTTTACGCAg > 1:577277/1‑229 (MQ=255)
gggTGGAAAAAAAGAGTGTGCCACCCGCCCATTTCACCCTCCTTGTGGTGAATGCTTTAGGTGAACGGCCTGGGTTCGTTCTCACCTACGTTACCGCCGATTTCTACCAGTTCTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTATTTACGCAGAACGt < 2:640212/228‑1 (MQ=255)
ggTGCAAAAAAAGAGTGTGCCCTCGGCCAATTCCAACAGCCATCTGGCGAACCGCTTCGAGGAACGGCAAGGATTCGATATCACCTCCTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTc < 1:249159/229‑1 (MQ=255)
gTGCATAAACAGATTGTGCCCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTCCTGTTCCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTc < 1:585380/228‑1 (MQ=255)
gagTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGCATTCGATATCACCGACTGTAACGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGCGATGTGCGGACTAACCTGCACGGTTGGGCCGCGGTAGACACCGCCGCGTTCGTTACGCAAACAGTCAGGGTAGATAca > 2:636658/1‑228 (MQ=255)
agTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCCCGCGCTCTTTGATTGCGTTAGTGATGTGGGGAATAACCTGCACGGTTGCGCCGGGGTAGTCACCGCGGCGTTCTTTACGCAAAACGCCAGGGTAGATAcc > 2:585379/1‑227 (MQ=255)
cGAGGAACGGCAAGGATTCGATATCCCCTACTGTTCCGCCGATTTCTGACAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGa < 1:363388/228‑1 (MQ=255)
aCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCTGGGTGAAGTCGTTGCGGCGGCGCCATTTGGTCAGAAGGGAACGCTCGTGGTGCCcccgggaccgc > 1:689371/1‑223 (MQ=255)
aCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCAATTTGGGACCAAAGAAACGCGCGAAGTGGCccatgtcccg > 1:444381/1‑226 (MQ=255)
|
GCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGG > NC_000913/2909002‑2909457
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
