Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R1
|
43 |
55.2 |
1410130 |
81.3% |
1146435 |
215.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,398,941 |
A→G |
28.3% |
S346S (TCT→TCC) |
mreC ← |
cell wall structural complex MreBCD transmembrane component MreC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,398,941 | 0 | A | G | 28.3%
| 49.0
/ 2.1
| 32 | S346S (TCT→TCC) | mreC | cell wall structural complex MreBCD transmembrane component MreC |
| Reads supporting (aligned to +/- strand): ref base A (3/19); new base G (6/4); total (9/23) |
| Fisher's exact test for biased strand distribution p-value = 1.26e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGAT > NC_000913/3398719‑3399161
|
gATGGGGCAAGGCAAGGAGCCAATACAACAAGAGGGGTAACACCCAGTTGGGCCGGAAAACAATGAGGTTATCCGGGCAGGTCAGGACTTGCAGCAGCAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCTTCCCTGGCTACGATAGCTCGCCTCTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCTCTTTGTGGAGAGCg < 2:451240/228‑1 (MQ=255)
tGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTTGGCACCACTTTGCGGCGGCGTA‑GCCCCTTGTGGGGAGCggg > 1:175955/1‑226 (MQ=255)
tGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGCTGAGCACCCCTTTGCGGCGGCCGA‑CCCCCTTGTGGagaggggg > 2:31004/1‑224 (MQ=255)
ggcgCCAGAGTCCAAGACAAAAAGAGGTGCAACACCTGGTGCGGCCGGAAAACAAGCAGGTTACCCGGCCAGGGCACGATTTGCCGCAACCGCGCAATGAGGAAAGAGAGCCGGATTACCCAGCGTCCCTGGCTGCGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTagcag < 2:313209/225‑1 (MQ=255)
ccAGGTTGCAAGACACAAAGATGTGTGAAACCCTGTTTGTCCGGAAAAAAATCAGGTTGCCCGGCCAGGGCATGATTGGCAGCAACAGAGCAATGAGGAAAGAGGGGCAGATTACCCAGCGGCCCTGGCTACGATAGCTCGCCACTAGTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAgg < 2:344914/228‑1 (MQ=255)
ccAGGAACCAATACAACAAGAGGAGTAACCCCCATTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAgg < 2:410387/228‑1 (MQ=255)
aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGAAAGCTCGCCACTATTGCCCTCCCGGCGCACCCGCAGGCGGGTGAGCGCCCCTCTTCGGCGGCGCACGCCCCTCGCGGGGA‑CGGTTAgcagcaccgcggggcgg > 2:507467/1‑217 (MQ=255)
caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTTTGGGGACGGGTTAGCAGCCGGCGGGGTCGgggcgcca > 1:3018/1‑224 (MQ=255)
caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCCGCCCA‑GGCCCTCCTGGAGAGGGCGCAGCAGCAGGCCGTGTCTGCgccgca > 2:657055/1‑228 (MQ=255)
gATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGGTGAGGACCACTTTGCGGCGGCGTA‑GCCCCTTGTGGGGAGCGGTTAGCCGCAGGCTTGGGCGGCGCCGCAgg > 1:400019/1‑227 (MQ=255)
gCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCCGCGGCCTA‑GCCCCTTGTTGGGAGCGGGTAGCAGACAGCTGTGTCGGGCCAGCCGGCGCCGTAGC‑CGCATTTcc > 1:497150/1‑225 (MQ=255)
tCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCCCCTTTAGGCGCCGGA‑GGCCCTTGTGGGGGGCGGGTAGCACCAGGCCGTGTCGGCGCAGCAGGCGCCGGAGCGGGCTTTTCCTG‑CGCCCGGTGcggcgggggc > 2:158895/1‑223 (MQ=255)
cGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGc < 1:31004/228‑1 (MQ=255)
gcgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTAGTGCCCCCCCGGCGCACGCGCAGGCGGTTGAGCTCCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAgg < 1:470593/228‑1 (MQ=255)
cgcAAGGGGGAAAGAGAGCCAGATTCCCCGGCGTCCCTGGCTACGATAGCCCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGt < 2:3018/228‑1 (MQ=255)
ccaTTCCCCCGCGTCCCCGGGTCCGATAGCTCCCCACTATTGCCCCCCCGGCGCCCGGGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGt < 2:79321/225‑1 (MQ=255)
aGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGt < 1:158895/227‑1 (MQ=255)
ccccGGCGCCCCTGGCTGCGATAGCTCGCCATTATTGGCCGCCCGGGGCACGCGCAGGCGGTTGAGCCCCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCg < 2:577729/227‑1 (MQ=255)
cccAGCGTCCATGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCg < 1:494912/227‑1 (MQ=255)
tcgtgtccccGCCCCTGTCGCCCCCCCGGCGCATGCGGAGGCGGTTGGGCCCCAGTTTGCGGTGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc < 2:599780/219‑1 (MQ=255)
cGATAGCTCGCCACTATTGCCCTCCCGGCGCTCGCGCAGGCGGTTGGGCACCCCTTTGGGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc < 1:523275/227‑1 (MQ=255)
ccGGCGCACGCGGGGGCGGTTGAGCCCCCCTTTGCGGCGGGGGG‑GCCGTTTGGGGAGAGGGAGTAGCAGCAGGCTGTGGCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAg < 2:497150/228‑1 (MQ=255)
gcatggtctggCGGTTGACGCCCTATTTTCGGTGGCGTA‑GCCGTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGTAGGCGCAGGAGC‑GGCATTTCCTGTCGCCGGTTGCTGCGGCGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAaca < 2:124569/219‑1 (MQ=255)
gcACGGGCAGGCGGTTGGGCACCACTTTGGGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGGGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAaca < 1:386881/228‑1 (MQ=255)
tgccccccttttGGGGCGGCGTA‑GCCCTTTGGGGGGGGCGATTCGCAGGAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATCCCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCg < 2:175955/219‑1 (MQ=255)
gggctcccgtttGGGGCGGCGTA‑GGCCTTTGTGGGAGGCGCTTTGCAGCCGGCTGGTGCGGCGGGGCCGGCGCAGGTGC‑TGCAGTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCg < 2:235173/219‑1 (MQ=255)
cttgggggcggccGA‑GGCCTTTGGGGAGGGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCCCAGTAGC‑TGCCTTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt < 1:652501/221‑1 (MQ=255)
atttggcgcggcgGA‑ACCCTTTGTGGGAGGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt < 1:154185/220‑1 (MQ=255)
cggcggcgTA‑GCCCTTTGTGGAGAGGGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGt < 1:696466/227‑1 (MQ=255)
gggcgTG‑GCCCGTTGTGGAGAGACGTTACCAGGAGGCTGGGTCGGCGCAGCAGGCGCAGTAGC‑TTCATTTCCTTTCGACGGTTTCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAg < 1:376678/226‑1 (MQ=255)
cggcgTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGTAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCCATACCTTCGGCATCTTCTGCCTCAGACGTCCCTTAGCAACACGCTGCACCTCTTCCGGCGTCATCGGGtttg > 1:106478/1‑225 (MQ=255)
cgaggaggccTTTTGGGGGAGCGATTAGCCGGAGGCTGTGGCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGGGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAgcg < 1:507467/220‑1 (MQ=255)
ttGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCCGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACCTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCCCCGttcacat > 1:380243/1‑222 (MQ=255)
|
GATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTA‑GCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGC‑TGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGAT > NC_000913/3398719‑3399161
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
