Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F9 I0 R1 74 77.5 1840812 85.2% 1568371 224.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,877,287 A→C 21.5% G82G (GGT→GGG)  yoaF ← DUF333 family outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,877,2870AC21.5% 89.1 / 2.1 58G82G (GGT→GGG) yoaFDUF333 family outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (20/22);  new base C (5/10);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGG  >  NC_000913/1877049‑1877508
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
aaCGGAACTGCGCAAAGCCTTTCCCGGCGAAGGCGGCGATTATGCAACCTTGCGCGAGCAATATCTTGCAGAACTGGCGCAACCCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCa                                                                                                                                                                                                                                   <  1:849149/239‑1 (MQ=255)
 ccGGAACTGCGAAAACGCTTTCCCGGCGAAGTCGCCCATTAGGAAACCTTTCGCGAGCAATATCTTGCAGAACGGGCGCAACAGGAGAAAGAAGGAAAGCGGCGGGCGGACACCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCGGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCAc                                                                                                                                                                                                                                  <  2:254527/238‑1 (MQ=255)
          cgcAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTTCCCAGCCGGAt                                                                                                                                                                                                                         >  1:650962/1‑239 (MQ=255)
             aaaCCCTTTCCCGGGGAATGCGTCGATGTTGCAACCTTTCGCGGGCAATATCTTGCAGAACGGGCGAAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGt                                                                                                                                                                                                                      <  2:785968/239‑1 (MQ=255)
               ctcttttCGCGGCGAATTCGTCAATTATGCACCCTTTGCCGAAAAATTTCTTGCGGAACGGGCCCAACGCGGGAAGGAAGGAAAGGGGCTGGCGGACATCGCCAAAAAACAGCCCAGGCCCCTGCACCACTCAGCAAAAAACACCACGCAGACCCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCCCCAGCCGGATGGTCa                                                                                                                                                                                                                    <  2:219033/235‑1 (MQ=255)
               aGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTCCGTAGCTCGTGATTTTTGAACAGCATCCGGCGGTTTATTTTTGACC‑GCCGGATGGTc                                                                                                                                                                                                                     >  1:216042/1‑238 (MQ=255)
                                 gggttgttATGGAACTTTTCGCGCGAAAATTCTGGCCAAACGGGGCAAACCCGGGCAAGAAGGAAAGCGGCTGGCGAAAATCGCCAAAAACCAGCCGTGGACCCGGCCTTACTCAGCAAAAAACACCCCGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCa                                                                                                                                                                                                   <  2:521330/232‑1 (MQ=255)
                                       ttATGCAACTTGGCGCGAGCAATACCTTGCAGAACTGGCGCAACACGGACAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATtcatc                                                                                                                                                                                             <  1:539147/238‑1 (MQ=255)
                                                 tttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAACCGGATGGTCACGCCGCCACACTGCCCCTTCATCAAACGtttaa                                                                                                                                                                                   >  1:125666/1‑236 (MQ=255)
                                                  ttCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGGGCTGGCCGACTGGCTACGTAACTTGTGATTTTAGTACAGCATCCGGCGG‑TTTTTTTTTCACACCCGGATGGTGAAGCCCCCCAAATGCCCCTTCATCAAATGtttacc                                                                                                                                                                                  >  2:310186/1‑235 (MQ=255)
                                                         gCAATATCTTGCAGAACTGGCGAAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACCCCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCgg                                                                                                                                                                           <  1:319524/238‑1 (MQ=255)
                                                             tatCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAGGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGcc                                                                                                                                                                              <  1:171076/231‑1 (MQ=255)
                                                                     aGAACGGGCGCAACCCGGGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCAGGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAAtt                                                                                                                                                               <  1:911398/238‑1 (MQ=255)
                                                                        aCTGGCGCACACCGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAACCAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAAttgttg                                                                                                                                                           <  1:310186/239‑1 (MQ=255)
                                                                         cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑GTATTTTTTACCACCCGGCTGGGCACCCCGCCCCAACGCCCCTTCCTCATTCTCTTTTCCCCCCGCGACTTTTCCATTGTTGc                                                                                                                                                          >  2:591156/1‑239 (MQ=255)
                                                                           tgcgcAACCCGCGCAAGAAGGAAAGCGGCTGGCGGAAATCGCCAAAAAACAGCCGCTGACCCTGCTCTCCTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTg                                                                                                                                                        <  2:283121/238‑1 (MQ=255)
                                                                           ggCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGAACCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCCTTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTg                                                                                                                                                        >  1:133189/1‑239 (MQ=255)
                                                                              gcAACACGAGCAAGAAGGAAAGCGGCTAGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTCCAGCATCCGGCGG‑TTATTTTTCACCCGCCGGATGGTCACGCCGCCCCAATGCCCATTCATCAAACGGTTCCCCGCCCGGTAATTTGCAAATTTTGCTTA‑c                                                                                                                                                     >  1:382435/1‑238 (MQ=255)
                                                                              ccAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCAAAAAAACAGCCGCTGCCCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑c                                                                                                                                                     <  1:535697/237‑1 (MQ=255)
                                                                                     gtcaaagaagGAAAGCGGCGGTGGGACCTCCGCAAAAACCACCCGGGGACCCTGTACTACCCAGCAAAAAACACCCCGCAGAACCATGCGCGGGTGCTGGCCGACTGGCAACGTAGCTTGGGATTTGAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTgcg                                                                                                                                             <  2:560969/235‑1 (MQ=255)
                                                                                      aGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCTCCCGCTAATTTGCCATT‑TTGTTCA‑ccccctttc                                                                                                                                             >  1:914657/1‑232 (MQ=255)
                                                                                      aGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTAACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGGAATTTGCAATTGTTGCTTA‑CCCCTTgc                                                                                                                                              >  1:123403/1‑237 (MQ=255)
                                                                                                tcaGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCCCCACAATGCCCCTTCCTCAATCGGTTTACCCCCCGGGAATTTGCAATTGTTTCTGACCCCCTTGCG‑TGTCTG‑c                                                                                                                                    >  1:857622/3‑236 (MQ=255)
                                                                                                aaaggcgCTGGCGGCAATCGCAAAAAACCAGCCGCTGACCCTGTTCTACTAAACAAAAAACACCCCGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCCTCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑Ca                                                                                                                                   <  1:82583/231‑1 (MQ=255)
                                                                                                 aaGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑GTATTTTTTTCCCACCGGCTGGGCACGCCGCAACAAAGCCCCTTCATCAATCGTTTCTCCCCCCCGGAATTTTCAATTGTTGCTCA‑CCCCTCGCCCTGTCtctcctg                                                                                                                                 >  2:528836/1‑233 (MQ=255)
                                                                                                   gCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGCCTGGCTACGTAGCTTGGGATTTTAGTACAGCATCCGGGGG‑TTTTTTTTCCACAACCGGATGGTTACGCCGCCACAATGCCCATTCCTCAAACGTTTCACCGCCCGGCAATTTTCCATTGTTGCTTA‑CCCCTTCCCCTGCCtcccccgga                                                                                                                               >  2:528861/1‑231 (MQ=255)
                                                                                                       cTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTTA‑CCCCTTGCGCTGTCTG‑CACTGGAATg                                                                                                                           >  1:728330/1‑238 (MQ=255)
                                                                                                         ggCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCCCAATTCCCATTCATCAATCGTTTCACCCCCCGGGAATTTGCAAtt                                                                                                                                                               >  1:700604/1‑202 (MQ=255)
                                                                                                             gcaacgccaaaaaaaCCGCCGGGGACCCTGCTTTCGTCAGAAAAAACCACCACGCAGAACCATGGGCTGGTGCTGGCCGACTGGCTAGGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGAGGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTc                                                                                                                     <  2:799427/229‑1 (MQ=255)
                                                                                                             cacacccccAAAAAACCCCCGCTGCCCCTGCTCTCCCCCGCAAAAAACCCCCCGAAAAACCATGCGCTGGTGGTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCACCCGGCGG‑TTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTcc                                                                                                                    <  2:268738/232‑1 (MQ=255)
                                                                                                                        aaacCAGCCGCTGCCCCTGCTCTACTAAGCAAAAAACCCCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGGTATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTctgc                                                                                                         <  1:591156/239‑1 (MQ=255)
                                                                                                                                        ctcttctACCCACAAAAAACCACCCCGCAGAACCAGGGCGTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACg                                                                                         <  2:650962/235‑1 (MQ=255)
                                                                                                                                             ccaCTGAGGAAAAACCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGGACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCgta                                                                                    <  1:528836/237‑3 (MQ=255)
                                                                                                                                                cTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCCCCCGCCGGATGGTCACCCCCCCCCAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTTCAATTGTTGCCGC‑CCCCTTTCCCTGTCTG‑CCCTGGAATGGGCGTCCCCCCCCTTTCCTGGCAAAAAACACCGGCCGGAttt                                                                                 >  2:5054/1‑239 (MQ=255)
                                                                                                                                                   aGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTTA‑CCCCTTGCGCTGCATGCCACT‑GACTGAACGTCCCGCCCTCCGTCTGGCCATAAGCCGCGCCCGGGTTTGc                                                                               >  2:243509/1‑238 (MQ=255)
                                                                                                                                                     cAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCCATTTTTGCTGA‑CCCCTTTCGCTGTCTT‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCAGGGATTGCCAt                                                                            >  2:202619/1‑239 (MQ=255)
                                                                                                                                                     cAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTTTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGGATTAGCGTCCCGCCCTTTTGCCGGCAAATAACCCACCCCGGCTTTGCCaa                                                                            >  2:324737/1‑238 (MQ=255)
                                                                                                                                                      aaaaacCCCCCCCCAAAACCATGCCCTGGTTCGGGCCGACTGGCTACGGAGCTTGTGATTTTAGTACAGCACCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATa                                                                           <  2:857622/239‑1 (MQ=255)
                                                                                                                                                        aaaaCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATAcc                                                                         >  1:575010/1‑239 (MQ=255)
                                                                                                                                                          aaCACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCCCCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTTCCATTCCaa                                                                       >  1:214289/1‑239 (MQ=255)
                                                                                                                                                               cacGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATTAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCttc                                                                  >  1:28631/1‑237 (MQ=255)
                                                                                                                                                                 cgcgGAACCAGGCGCGGGTGCTGGCCGACTGGCTACGTAGGTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTgcgg                                                                <  1:727722/239‑1 (MQ=255)
                                                                                                                                                                 cGCAGAACCAGGCGCGGGGGCTGGCCGACGGGCGACGTAGCTTGTGATTTTAGTACAGCAACCGGCGG‑TTATTTTTCACCAGCCGGATGGGCACGCCGACACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTGGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTgcgg                                                                <  1:614830/239‑1 (MQ=255)
                                                                                                                                                                  gCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTgcgg                                                                <  1:649710/238‑1 (MQ=255)
                                                                                                                                                                      acccATGCGCGGGTGCTGGCCCACGGGCTACGTAGCTTGTGATTTTAGTCCAGCACCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGt                                                           <  1:210317/236‑1 (MQ=255)
                                                                                                                                                                           tGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCCATTGTTGCTGA‑CCCCTTTCTCTGTCTG‑CCCTGGAATGAGCGTCCCGCCCTTCTGCTGGCCCACGACCGACGCCGGGTTTCCCCTACCAAACTTcggg‑ggggttagg                                                     >  2:649710/1‑232 (MQ=255)
                                                                                                                                                                           tGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGAc                                                                                              >  2:47036/1‑199 (MQ=255)
                                                                                                                                                                           tGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGAc                                                                                              <  1:47036/199‑1 (MQ=255)
                                                                                                                                                                             cgcTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTCCGCC‑GGTTTTggggg                                                   >  1:350484/1‑236 (MQ=255)
                                                                                                                                                                                tGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTc                                                <  1:202619/239‑1 (MQ=255)
                                                                                                                                                                                tGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCCCCCGGTAATTTTCAATTTTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCCCCCTTCTGCTTGCAATCAACCGCAGCCGGATTTGCCCTACCAACCTCCGCCGGGTTTAgtgtacc                                                 >  2:560853/1‑233 (MQ=255)
                                                                                                                                                                                          ccaCTGGCTCCGTGTGTTGTATTTTTATTCCCGCCCCCGGGGT‑TTTTTTTCCCCCACCCGGATGTGCCCGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTgag‑a                                      <  2:568139/236‑1 (MQ=255)
                                                                                                                                                                                                                   ttgtccAGCCTCCGGGTG‑TTCTTTTTCCCCAGCCGGAGGGTCACGCCGCCACAATGCCCCTTCACCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCa            <  2:728330/234‑1 (MQ=255)
                                                                                                                                                                                                                   tggTACCGCCTCGGGGGT‑TTTTTTTTCCCCAGCCGGATGGTCACCCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCa            <  2:186625/237‑1 (MQ=255)
                                                                                                                                                                                                                   atgtcccgagaccGGTGG‑TTATTTTCCCCGACCGGGATGGCCACGCCGCCACCATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGCCCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCa            <  1:362162/228‑1 (MQ=255)
                                                                                                                                                                                                                        cAGCATCCGGCGG‑TTATTTTCCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCagcaag       <  1:428626/239‑1 (MQ=255)
                                                                                                                                                                                                                           cATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTATGGGGTTTCGGCTGCGAAAGGGGGTGAAAAACC‑CCCCGGACCCgcaaacaa    >  1:186625/1‑239 (MQ=255)
                                                                                                                                                                                                                             cccGGTGT‑TTTTTTTTCCCCAGTCGGTGTGTCCCGCCGCCACAATGCCCTTTCATCAATCGTTTCCCCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAgg  <  2:123403/238‑1 (MQ=255)
                                                                                                                                                                                                                             cccGGCGT‑TTTTTTTCCCCCCGCCGGATGGTCACCCCGCCACAATGCCCATTCCTCCATCGTTTCCCCGCCCGTTAATTTGCAATTGTTGCTTA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAgg  <  2:912761/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
AACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGG‑TTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCAATTGTTGCTGA‑CCCCTTGCGCTGTCTG‑CACTGGAATGAGCGTCCCGCCCTTCTGCTGGCAATAGACCGACGCCGGATTTGCCATACCAATCTGCGGC‑GGTTTAGGTGCTTCTGGCTGAG‑AAGGGGTTGAACAACCAGCCAGGACCAGCAAGCAAGG  >  NC_000913/1877049‑1877508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A