Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 177 96.4 2178490 87.4% 1904000 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,795 A→C 21.4% V454G (GTG→GGG)  speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,7950AC21.4% 84.8 / 4.0 54V454G (GTG→GGG) speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (20/19);  new base C (3/11);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 6.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACAGAATGGAGTTGAGAT‑CGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGA  >  NC_000913/3107557‑3108008
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                        
aaaCAGAAGGGGGTTGGGATGCGC‑TTTCTTCGGGGCAATGCCGTTCTGCCGGCGGTGGTGCGCCAGAATCGGCGGCGGAAGGCCAAAGTCGCTATATTCGCCGGGTTCGGCATCGATACCTGGTGTAGTGAGTAACTGCTTGCACGGATCAACAAAATACTGATCCGCGGCATGTCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAaca                                                                                                                                                                                                                          <  2:79985/239‑1 (MQ=255)
      aaTGGAGTTGAGAT‑CGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAacaccgga                                                                                                                                                                                                                     >  1:766398/1‑234 (MQ=255)
                  aT‑CGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGCCGGTCGCT‑GGGTAACACTGATGTGGGGTAATccc                                                                                                                                                                                                        >  1:397981/1‑238 (MQ=255)
                      gCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AACAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGGTAATCCTGc                                                                                                                                                                                                      >  1:767621/1‑238 (MQ=255)
                      gCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑TGCGAACACTGATGTCGGATAATCCTGc                                                                                                                                                                                                      >  1:294041/1‑238 (MQ=255)
                                       tGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAACGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGCACGTCCCT‑TGCTAACACGTAAGTCCGTTATTCCTGCCCCACTTTGCCATcaaca                                                                                                                                                                                    >  1:497940/1‑239 (MQ=255)
                                       tGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCCCCCCCGGCCCAAAACTG‑AAAAAACGGCGGGCCCC‑GGCCAAACCCTCTGTCGGGTAATCCTGCCACAAATTTCCacccaaa                                                                                                                                                                                    >  2:1072318/1‑233 (MQ=255)
                                             tcccACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAAcggg                                                                                                                                                                               <  1:745743/235‑1 (MQ=255)
                                              ctcACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGCCGGTCGCT‑TGCTTACACTGATGTCGGATAATCCTGCCCCAATTTGCCATCAACAAcggggc                                                                                                                                                                             >  1:1082218/1‑237 (MQ=255)
                                                         agggcgcAAGAATCGGCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACggg                                                                                                                                                                  <  1:790283/236‑1 (MQ=255)
                                                         aGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGCCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCCCACTTTGCAATCAACAACGGGCGGGGTAAACcgg                                                                                                                                                                  >  1:950915/1‑239 (MQ=255)
                                                               ccAGAATGCTCGCCGGAACGCCAAAGTCGTTATTTTCCCCGGTTTCGGCATCGATCCCTGGTGTAGTGAGTAACAGCTTGCCCGGACCAACAAAATACTGACCCGCGGCATAGCCTTCAAAGCCGGGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAAc                                                                                                                                                            <  2:511701/239‑1 (MQ=255)
                                                                     tcgtcgCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGCACCG‑GGCCAACACCGAGGTCGGGTAAACCTTCCCCAATTTTCCCCCAACAACCGCCGGGAGAAACGGGGGGaaaaactga                                                                                                                                                      >  2:934242/1‑232 (MQ=255)
                                                                          gCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGAGAATCCTGCCCCAAATTGCCATCAACAACGGGCGGGGTAAACGGGCGGGAACGCTTAAAgc                                                                                                                                                  >  1:892711/1‑238 (MQ=255)
                                                                                           gtttttttCGCCGGTTTCGGCAGTGGTCCCTGGTGTAGGGATTAACAGCTTGCCGGGATCAACAAAATACTGCTCCGCGGCATACCCGTCAACGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgc                                                                                                                                <  2:791281/233‑1 (MQ=255)
                                                                                             tataTTCGCCGGTTTCGGCATCGATACCTGGTGTAGGGAGTACCAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                              <  1:106632/239‑1 (MQ=255)
                                                                                             tagatTCGCCGGTTTCGGCATCGATCCCTGGTGTAGGGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                               <  1:641629/235‑1 (MQ=255)
                                                                                                      ggttttCGGAGTCGTTTCCTGGGTTAGTGAGTAACGTGTTGCAGGGAACAACAAAATACTGGTCCGCGGCAAATCCTTAAAAGCCGGGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATc                                                                                                                     <  2:294041/236‑1 (MQ=255)
                                                                                                           tCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAACTTACATCACGCAAAGAAAGCCTTTCGCGCTTCCAACCCTa                                                                                                                 >  1:908171/1‑238 (MQ=255)
                                                                                                                  cGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGACGCT‑GGCCAACACTGATGTCCGGAAATCCTGCCCCAAATTGCCCTCCAAAACGGGCGGGGTAAAAGGGCGGAACAACCTAACGCGCCCCAAAAAAACCTTTCGCGCTTCCATCCCAAttccacaa                                                                                                         >  2:819351/1‑232 (MQ=255)
                                                                                                                   gATGCCGGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGGTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑Aacac                                                                                                       <  1:696236/239‑1 (MQ=255)
                                                                                                                      aCCTGGTGTAGTAAGTAACAGTTTGCACGGATCAACAAAATACTGGTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCACGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACt                                                                                                    <  1:209816/239‑1 (MQ=255)
                                                                                                                              tAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGAGCCGCGGATTATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTAAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCca                                                                                             <  1:230829/238‑1 (MQ=255)
                                                                                                                                 tGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGTGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTTCGCGCTTCAATCCCTATCTC‑CACACACCCGACCCACAg                                                                                          >  1:731461/1‑238 (MQ=255)
                                                                                                                                  gAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGGCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑CACACACTCAGCCCACAccc                                                                                        >  1:258966/1‑239 (MQ=255)
                                                                                                                                   aGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAAACCTGTTGTCGGATAATCCTGCCACAATTTGCCCTCAACAACCGGCGGGATAAAAGGGCGGCACAGCTTAAAGCGCGCAAGAATAGCCCTGCCGCCCTCAATCCCCATCTC‑AAAACACCCAACCcaccacc                                                                                        >  2:662358/1‑234 (MQ=255)
                                                                                                                                   aGTAACAGCTTGCACGGAAAAACAAAATCCTGACCCGCGGCATACCCTTCAAAGCCGGGCCCCTTCGCCCCCGGCTCAAAACGG‑AAAAAACGGCGGTCGCT‑GGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                 <  2:523852/214‑1 (MQ=255)
                                                                                                                                     tAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGCGGGGGTAAACGGGCGGAACAGCTTACCACGCGCACGAAAAGCCCTGTGCGCCTCAACCCCCACCTCAAACACACCCACCCCACACCCg                                                                                       >  2:725771/1‑238 (MQ=255)
                                                                                                                                     tAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑Aac                                                                                                         <  1:1025641/219‑1 (MQ=255)
                                                                                                                                       aCAGCTTGCCCGGAACAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGTGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGc                                                                                   <  1:217448/239‑1 (MQ=255)
                                                                                                                                               cACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑CACACACTCAGCCCCCAGCCGCCCCCCCcttc                                                                            >  1:974858/1‑236 (MQ=255)
                                                                                                                                                aCGGATCAACAAAATACTGATCCGCGGCATATCCCTCAAAGCCGTGCCACTTCGCCCCCGGCTAAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTcc                                                                          <  1:387315/239‑1 (MQ=255)
                                                                                                                                                       aacaaAAAACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                   <  1:1072318/239‑1 (MQ=255)
                                                                                                                                                         caaAATACTGATCCGCGGGATATCCTTCAAAGCCGTGCCACTTCCCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACCCGGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGcc                                                                                  <  1:977797/222‑1 (MQ=255)
                                                                                                                                                              tACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGGTAAACGGGCGGAACAGCTTAAAGCGCGCAAGAAAAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACCCCCCCCCCCCCCCTCCTCAA‑tttt                                                           >  1:133068/1‑239 (MQ=255)
                                                                                                                                                              tACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGAAAATCCTGCCACAATTTGGCATCAACAACGGGCGGGATAAACAGGCGGAACAGCTTACAGCCCGCCAGAATAGCCCTGCGCGCTTCAATCCCTCACCC‑AACACACCCAACCCCCAACCCACACCCCCCCCCCCCCTCCTGaaaatt                                                            >  2:629831/1‑235 (MQ=255)
                                                                                                                                                                 tGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACCGATGTCGGGTAATCCTGCCACCATTTGCCCTCAACAACGGGCCGGCTAAACGGGCGGAACACCCTACAACCCGCAAGAACACCCTTTCACGCTCCAACCCCTCTCTC‑AACCCACTCACCACCCACCCCGCGCCCCCCCCCCCCCTCATCAA‑tttttgg                                                        >  2:374554/1‑236 (MQ=255)
                                                                                                                                                                  gctCCGCGGCATATCCTGCAAAGCGGGGCCCCTTCCCCCCCGGCTAAAAACGG‑AAAAAACGGGGGGCGAT‑GGCTAAATCTGAGGGCGGATAAACCTGCCACAAGTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTGGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCg                                                       <  2:951100/237‑1 (MQ=255)
                                                                                                                                                                  gATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCCCAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAACTTACAGCGCGCAAAAAAAGCCCTGCGCGCTTCAATCCCTATCTC‑AACACACCCCGCCCCCACCCGCCCCCCCCTCCCCCCCTCACTAA‑TTTTgcgg                                                       >  2:747028/1‑236 (MQ=255)
                                                                                                                                                                   aTCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGCTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGGTAAACGGGCGGAAAAGCTTACAGCCCGCCAGAAAAGCCTTGCGCGCTTCAAACCCCATCTC‑AACACACCCACCCCCCAGCCCACCCCCCCCCCCCCCCTCATAAA‑TTTTGGGGt                                                      >  2:543009/1‑239 (MQ=255)
                                                                                                                                                                        cgGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCACGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCCTGAA‑TTTTTGCGTTAAc                                                  >  1:592863/1‑238 (MQ=255)
                                                                                                                                                                           caattCCTTCAAACCCGTGCCGCTTCGCCCCCGGCTCAAAACTA‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATc                                               <  1:819351/234‑1 (MQ=255)
                                                                                                                                                                           cATATCCCTCAAAGCCGTGCCACTCCCCCCCCGGCCAAAAACTG‑AAAAACGGGCGGGCGCT‑GGCTAACACTGATGTCGGAAAACCCTGCCACAATTTGCCATCAAAAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATc                                               <  2:1082218/238‑1 (MQ=255)
                                                                                                                                                                                ccTTCAAAGCCGTGCCACTTCGACCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGGT‑GGCTAACACTGAGGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTgc                                        <  1:374554/239‑1 (MQ=255)
                                                                                                                                                                                 cTTCCAACCCGGCCCCCTTCCGCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GTCTAACCTTGATGTCGGAAAACCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTgca                                       <  2:767621/239‑1 (MQ=255)
                                                                                                                                                                                 cTTCAAATCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTgca                                       <  1:320414/239‑1 (MQ=255)
                                                                                                                                                                                          cgggCCACTTCGCCCCCGGCTAAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGGCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACa                                <  1:975320/234‑1 (MQ=255)
                                                                                                                                                                                             gCCACTTCGCCCCCGGCTCAAAACTG‑AAAAACCGGCGGTCGCT‑GGCTAACCCTGATGCCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCgg                            <  1:158314/238‑1 (MQ=255)
                                                                                                                                                                                                    cGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTTACACTGATGTCGGATAATCCTGCCACAATTTGCCCTCAACAACGGGCGGGATAAACGGGCGGAACAGCCTAAAGCCCGCCAAGATAGCCTTGCGCGCATCCATCCCCTTCTC‑CACACACTCAGCCCACAGCCTACCCCCACCGTTCCCCTCATTAATTTTTCGCGTT‑ACATACAGGTGCAGAAAACcgcgaaaaaaaa                     >  2:365524/1‑231 (MQ=255)
                                                                                                                                                                                                      ccccgGGCT‑AAAACTGAAAAAAACGGCGGCGGTG‑GGATAACCCGGAGGCCGGATAACCCTGCCAAAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAAACCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAggg                  <  2:133068/239‑1 (MQ=255)
                                                                                                                                                                                                      cccccGGTTCAAAACGA‑AAAAAACGGGGGT‑GCTGGGATAAACCGGATGGCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGATTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAggg                  <  2:1045789/239‑1 (MQ=255)
                                                                                                                                                                                                      cccccGGCTCAAAACTA‑AAAAACCGGCGGTCGCG‑GGCTAACCCGGAGTTCGGATAACCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAggg                  <  2:908171/239‑1 (MQ=255)
                                                                                                                                                                                                       ccccGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAGGGc                 <  1:662358/239‑1 (MQ=255)
                                                                                                                                                                                                                  aaCTG‑AAAAACCGGGGCGCGCT‑GGCAAACACGGATGTGGGGTAACCCTGCCACAATTTGCCATAAACAACGGGCGGGAAAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa      <  2:974858/239‑1 (MQ=255)
                                                                                                                                                                                                                   accgcaaaaaaCGGCCGTCGCT‑GGTTAACCCGTTTGTAGGAAAGTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTTGCTC‑AACCCACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa      <  2:497940/234‑1 (MQ=255)
                                                                                                                                                                                                                      g‑AAAAAACGGCGGTCGCT‑GGCTAACCATGATGTCGGATAATCCTGCCACAATTTGCCATCAACCACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa  <  1:629831/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                        
AAACAGAATGGAGTTGAGAT‑CGCACTTCTCCGGCACAATGCCGTTCTCACGCAGATAGTGCGCCAGAATCGTCGCCGGAACGCCAAAGTCGCTATATTCGCCGGTTTCGGCATCGATACCTGGTGTAGTGAGTAACAGCTTGCACGGATCAACAAAATACTGATCCGCGGCATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTG‑AAAAAACGGCGGTCGCT‑GGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTC‑AACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAA‑TTTTGGCGTTAACATCCA‑GTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGA  >  NC_000913/3107557‑3108008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A